BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0252 (630 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 99 6e-22 SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 71 2e-13 SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 68 1e-12 SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr... 58 1e-09 SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||... 55 7e-09 SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 54 2e-08 SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 54 2e-08 SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr... 49 6e-07 SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|ch... 33 0.034 SPAC16.01 |rho2||Rho family GTPase Rho2|Schizosaccharomyces pomb... 31 0.18 SPAC23C4.08 |rho3||Rho family GTPase Rho3|Schizosaccharomyces po... 30 0.24 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 29 0.42 SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch... 29 0.42 SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch... 27 1.7 SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces p... 27 1.7 SPAC1F7.04 |rho1||Rho family GTPase Rho1|Schizosaccharomyces pom... 27 2.2 SPBC8D2.11 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.2 SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 25 9.0 SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 25 9.0 SPAC1783.03 |fta2|sma2|Sim4 and Mal2 associated |Schizosaccharom... 25 9.0 SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 25 9.0 >SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1|||Manual Length = 214 Score = 98.7 bits (235), Expect = 6e-22 Identities = 47/56 (83%), Positives = 51/56 (91%) Frame = +3 Query: 252 VRFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYYRGA 419 +RFTRNEFN+ESKSTIGVEFATR+I +D K IKAQI TAGQERYRAITSAYYRGA Sbjct: 28 MRFTRNEFNIESKSTIGVEFATRNIVLDNKKIKAQIWDTAGQERYRAITSAYYRGA 83 Score = 81.8 bits (193), Expect = 7e-17 Identities = 41/70 (58%), Positives = 48/70 (68%) Frame = +2 Query: 419 VGALLVYDIAKHLSYENVXXXXXXXXXXXXQNILIMLVGNKSDLRHLRSIXTEXAQAFAE 598 VGAL+VYDI K S++NV NI+IMLVGNK+DL HLR++ TE AQAFA Sbjct: 84 VGALIVYDITKQSSFDNVGRWLKELREHADSNIVIMLVGNKTDLLHLRAVSTEEAQAFAA 143 Query: 599 ANGLSFIETS 628 N LSFIETS Sbjct: 144 ENNLSFIETS 153 Score = 34.7 bits (76), Expect = 0.011 Identities = 14/16 (87%), Positives = 15/16 (93%) Frame = +1 Query: 178 REDEYDYLFKVVLIGD 225 +EDEYDYLFK VLIGD Sbjct: 3 QEDEYDYLFKTVLIGD 18 >SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 70.5 bits (165), Expect = 2e-13 Identities = 32/56 (57%), Positives = 42/56 (75%) Frame = +3 Query: 252 VRFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYYRGA 419 +RF+ + F +TIG++F R+IE+DGK IK QI TAGQER+R IT+AYYRGA Sbjct: 27 LRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGA 82 Score = 48.8 bits (111), Expect = 6e-07 Identities = 23/70 (32%), Positives = 36/70 (51%) Frame = +2 Query: 419 VGALLVYDIAKHLSYENVXXXXXXXXXXXXQNILIMLVGNKSDLRHLRSIXTEXAQAFAE 598 +G LL+YD+ S++NV +N+ +L+GNK D R + E QA A+ Sbjct: 83 MGILLLYDVTDKKSFDNVRTWFSNVEQHASENVYKILIGNKCDCEDQRQVSFEQGQALAD 142 Query: 599 ANGLSFIETS 628 G+ F+E S Sbjct: 143 ELGVKFLEAS 152 >SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 68.1 bits (159), Expect = 1e-12 Identities = 31/56 (55%), Positives = 41/56 (73%) Frame = +3 Query: 252 VRFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYYRGA 419 +RF + + STIGV+F R+ E++GKT+K QI TAGQER+R ITS+YYRGA Sbjct: 26 LRFADDTYTESYISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSYYRGA 81 Score = 43.2 bits (97), Expect = 3e-05 Identities = 21/69 (30%), Positives = 36/69 (52%) Frame = +2 Query: 422 GALLVYDIAKHLSYENVXXXXXXXXXXXXQNILIMLVGNKSDLRHLRSIXTEXAQAFAEA 601 G ++VYD+ S+ NV + + +LVGNKSD+ + + A+ FA++ Sbjct: 83 GIIIVYDVTDQDSFNNVKQWLQEIDRYAVEGVNRLLVGNKSDMVDKKVVEYSVAKEFADS 142 Query: 602 NGLSFIETS 628 + F+ETS Sbjct: 143 LNIPFLETS 151 Score = 29.1 bits (62), Expect = 0.55 Identities = 11/13 (84%), Positives = 13/13 (100%) Frame = +1 Query: 187 EYDYLFKVVLIGD 225 EYDYLFK++LIGD Sbjct: 4 EYDYLFKLLLIGD 16 >SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr 1|||Manual Length = 208 Score = 58.0 bits (134), Expect = 1e-09 Identities = 24/55 (43%), Positives = 38/55 (69%) Frame = +3 Query: 255 RFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYYRGA 419 +F +F+ E K+TIG +F T+ + VD K + Q+ TAGQER++++ A+YRGA Sbjct: 27 QFVNQKFSREYKATIGADFLTKDVVVDDKLVTLQLWDTAGQERFQSLGMAFYRGA 81 >SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2|||Manual Length = 205 Score = 55.2 bits (127), Expect = 7e-09 Identities = 22/55 (40%), Positives = 38/55 (69%) Frame = +3 Query: 255 RFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYYRGA 419 ++ +F+ + K+TIG +F T+ + VD K + Q+ TAGQER++++ A+YRGA Sbjct: 27 QYVNRKFSKDYKATIGADFLTKEVLVDDKVVTLQLWDTAGQERFQSLGVAFYRGA 81 >SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 211 Score = 54.0 bits (124), Expect = 2e-08 Identities = 24/57 (42%), Positives = 39/57 (68%), Gaps = 1/57 (1%) Frame = +3 Query: 252 VRFTRNEFNLESKSTIGVEFATRSIEVDGKT-IKAQIXXTAGQERYRAITSAYYRGA 419 +RF +++F+ +STIG F T+++ +D T +K +I TAGQERY+++ YYR A Sbjct: 32 LRFVKDQFDDYRESTIGAAFLTQTLPIDENTSVKLEIWDTAGQERYKSLAPMYYRNA 88 Score = 39.9 bits (89), Expect = 3e-04 Identities = 24/69 (34%), Positives = 34/69 (49%), Gaps = 1/69 (1%) Frame = +2 Query: 425 ALLVYDIAKHLSYENVXXXXXXXXXXXXQNILIMLVGNKSDL-RHLRSIXTEXAQAFAEA 601 A++VYDI + S E + I+I L GNK DL + R++ A+A+A Sbjct: 91 AIVVYDITQAASLEKAKSWIKELQRQAPEGIVIALAGNKLDLAQERRAVEKADAEAYAAE 150 Query: 602 NGLSFIETS 628 L F ETS Sbjct: 151 ANLLFFETS 159 >SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 54.0 bits (124), Expect = 2e-08 Identities = 26/59 (44%), Positives = 39/59 (66%), Gaps = 4/59 (6%) Frame = +3 Query: 255 RFTRNEFNLESKSTIGVEFATRSIEV----DGKTIKAQIXXTAGQERYRAITSAYYRGA 419 RF +N+++ + T+G++FA+R I V K IK QI TAGQE++R++ YYRGA Sbjct: 28 RFVKNQWDDQVSHTVGIDFASRIISVGMGNQQKRIKLQIWDTAGQEKFRSVARNYYRGA 86 Score = 42.3 bits (95), Expect = 6e-05 Identities = 21/64 (32%), Positives = 32/64 (50%) Frame = +2 Query: 422 GALLVYDIAKHLSYENVXXXXXXXXXXXXQNILIMLVGNKSDLRHLRSIXTEXAQAFAEA 601 GA+LVYD+ S+E + I ++L G+KSDL++ R + TE A F Sbjct: 88 GAVLVYDVTNKDSFEELSSWLSDIRAMAPSTICVVLAGSKSDLQNQRQVSTEEAAEFCSE 147 Query: 602 NGLS 613 +S Sbjct: 148 KHIS 151 >SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 201 Score = 48.8 bits (111), Expect = 6e-07 Identities = 18/53 (33%), Positives = 39/53 (73%) Frame = +3 Query: 255 RFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYYR 413 RF ++F+ ++TIG++F ++++ ++ +T++ Q+ TAGQER+R++ +Y R Sbjct: 30 RFMYDQFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIR 82 Score = 43.6 bits (98), Expect = 2e-05 Identities = 22/68 (32%), Positives = 35/68 (51%) Frame = +2 Query: 425 ALLVYDIAKHLSYENVXXXXXXXXXXXXQNILIMLVGNKSDLRHLRSIXTEXAQAFAEAN 604 A++VYDI H S+ N +++I+LVGNK+DL R + E + A+ Sbjct: 87 AIIVYDITNHNSFVNTEKWIEDVRAERGDDVIIVLVGNKTDLADKRQVTQEEGEKKAKEL 146 Query: 605 GLSFIETS 628 + +ETS Sbjct: 147 KIMHMETS 154 >SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|chr 2|||Manual Length = 185 Score = 33.1 bits (72), Expect = 0.034 Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 1/81 (1%) Frame = +2 Query: 389 RHHVGVLPRRVGALLVYDIAKHLSYENVXXXXXXXXXXXXQN-ILIMLVGNKSDLRHLRS 565 +H +G+ G +LVY I S+E V + I++VGNKSDL R+ Sbjct: 73 KHSIGIH----GYVLVYSITSKSSFEMVKIVRDKILNHTGTEWVPIVVVGNKSDLHMQRA 128 Query: 566 IXTEXAQAFAEANGLSFIETS 628 + E +A A ++ E S Sbjct: 129 VTAEEGKALANEWKCAWTEAS 149 >SPAC16.01 |rho2||Rho family GTPase Rho2|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 30.7 bits (66), Expect = 0.18 Identities = 19/63 (30%), Positives = 27/63 (42%) Frame = +3 Query: 231 CG*KQSSVRFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYY 410 CG FT F E T+ + + VDGK+++ + TAGQE Y + Y Sbjct: 19 CGKTSLLSVFTLGYFPTEYVPTVFENYVS-DCRVDGKSVQLALWDTAGQEEYERLRPMSY 77 Query: 411 RGA 419 A Sbjct: 78 AKA 80 >SPAC23C4.08 |rho3||Rho family GTPase Rho3|Schizosaccharomyces pombe|chr 1|||Manual Length = 205 Score = 30.3 bits (65), Expect = 0.24 Identities = 19/51 (37%), Positives = 25/51 (49%) Frame = +3 Query: 258 FTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYY 410 FT+ F + TI E I VDG +I+ + TAGQE Y + S Y Sbjct: 33 FTKGYFPQVYEPTI-FENYIHDIFVDGNSIELSLWDTAGQEEYDQLRSLSY 82 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 29.5 bits (63), Expect = 0.42 Identities = 14/37 (37%), Positives = 23/37 (62%) Frame = -2 Query: 263 SETDRRLLLPTPESPMSTTLNK*SYSSSLVPILVYPE 153 ++T R L+ P+P S +STT+NK + S+ + PE Sbjct: 2 TDTPRILITPSPLSDISTTINKPNVHPSMSLLKALPE 38 >SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr 1|||Manual Length = 219 Score = 29.5 bits (63), Expect = 0.42 Identities = 16/55 (29%), Positives = 27/55 (49%) Frame = +3 Query: 249 SVRFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYYR 413 +++ ++ F E TI + + E+DG+ + TAGQE Y A+ Y R Sbjct: 25 TIQLIQSHFVDEYDPTIEDSYR-KKCEIDGEGALLDVLDTAGQEEYSAMREQYMR 78 >SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr 2|||Manual Length = 216 Score = 27.5 bits (58), Expect = 1.7 Identities = 17/67 (25%), Positives = 33/67 (49%) Frame = +2 Query: 428 LLVYDIAKHLSYENVXXXXXXXXXXXXQNILIMLVGNKSDLRHLRSIXTEXAQAFAEANG 607 ++++D+ ++Y+NV +NI I+L GNK D++ R + + A F Sbjct: 86 IIMFDVTSRITYKNVPHWWRDLVRVC-ENIPIVLCGNKVDVKE-RKVKAK-AITFHRKKN 142 Query: 608 LSFIETS 628 L + + S Sbjct: 143 LQYYDIS 149 Score = 27.1 bits (57), Expect = 2.2 Identities = 15/52 (28%), Positives = 22/52 (42%) Frame = +3 Query: 255 RFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIXXTAGQERYRAITSAYY 410 R EF + +T+GVE + I + TAGQE+ + YY Sbjct: 28 RHLTGEFEKKYIATLGVEVHPLHFHTNFGEICFNVWDTAGQEKLGGLRDGYY 79 >SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 27.5 bits (58), Expect = 1.7 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 324 IEVDGKTIKAQIXXTAGQERYRAITSAYY 410 +EVDG+ I+ + TAGQE Y + Y Sbjct: 47 VEVDGRHIELALWDTAGQEDYDRLRPLSY 75 >SPAC1F7.04 |rho1||Rho family GTPase Rho1|Schizosaccharomyces pombe|chr 1|||Manual Length = 202 Score = 27.1 bits (57), Expect = 2.2 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +3 Query: 324 IEVDGKTIKAQIXXTAGQERYRAITSAYY 410 +EVDG+ ++ + TAGQE Y + Y Sbjct: 47 VEVDGRHVELALWDTAGQEDYDRLRPLSY 75 >SPBC8D2.11 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 122 Score = 25.8 bits (54), Expect = 5.2 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = -3 Query: 469 VLVRQVLGDIVHEQRAHAPR 410 VLVR+V G +RAH PR Sbjct: 65 VLVREVAGSFRQRKRAHMPR 84 >SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccharomyces pombe|chr 3|||Manual Length = 721 Score = 25.0 bits (52), Expect = 9.0 Identities = 17/51 (33%), Positives = 21/51 (41%), Gaps = 2/51 (3%) Frame = +3 Query: 195 LFVQSCTHWRFWCG*KQSSVRFTRNE--FNLESKSTIGVEFATRSIEVDGK 341 +FV+ TH W G K RF E N + VE A + DGK Sbjct: 274 IFVERKTHREDWTGEKSVKARFPLKEKYVNAFLRGDYTVEEAFAKMRKDGK 324 >SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1639 Score = 25.0 bits (52), Expect = 9.0 Identities = 12/45 (26%), Positives = 19/45 (42%) Frame = +3 Query: 369 AGQERYRAITSAYYRGAWARCSCTISPSTCRTRTWSGGCASCAXH 503 A + R+R+ T +YR + C + + T W G C H Sbjct: 930 AAEIRFRS-TDGHYRWHLVKSVCVNNSADTSTNLWLGTCTDIHDH 973 >SPAC1783.03 |fta2|sma2|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 1|||Manual Length = 351 Score = 25.0 bits (52), Expect = 9.0 Identities = 10/18 (55%), Positives = 14/18 (77%) Frame = +3 Query: 246 SSVRFTRNEFNLESKSTI 299 SS+RFT++EF ES+ I Sbjct: 155 SSIRFTQHEFEAESEKII 172 >SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1375 Score = 25.0 bits (52), Expect = 9.0 Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 3/54 (5%) Frame = -2 Query: 263 SETDRRLLLPTPESPM-STTLNK*SYSSSLVPILVYPELF--SRRQKSVSFETS 111 S T R++ L P+ S LNK +YS+ P+ ++ +L+ +RR K + TS Sbjct: 76 SFTQRKISLAKNLIPLFSVDLNKDTYSALKAPLKIWSKLWEDNRRLKKIIKYTS 129 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,217,487 Number of Sequences: 5004 Number of extensions: 40871 Number of successful extensions: 139 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 120 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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