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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0251
         (596 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g23890.2 68414.m03013 NHL repeat-containing protein contains ...    35   0.036
At1g23890.1 68414.m03014 NHL repeat-containing protein contains ...    35   0.036
At1g56500.1 68414.m06497 haloacid dehalogenase-like hydrolase fa...    32   0.33 
At5g65130.1 68418.m08193 AP2 domain-containing transcription fac...    30   1.3  
At1g23880.1 68414.m03012 NHL repeat-containing protein contains ...    27   2.2  
At5g14890.1 68418.m01746 NHL repeat-containing protein contains ...    29   2.4  
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ...    29   3.1  
At5g01840.1 68418.m00103 ovate family protein 59% similar to ova...    28   4.1  
At3g56690.1 68416.m06306 calmodulin-binding protein identical to...    28   4.1  
At3g50810.1 68416.m05564 integral membrane protein, putative con...    28   5.4  
At1g64500.1 68414.m07312 glutaredoxin family protein                   27   7.2  
At1g10390.1 68414.m01171 nucleoporin family protein contains Pfa...    27   7.2  
At4g24610.1 68417.m03525 expressed protein                             27   9.5  
At4g00200.1 68417.m00021 DNA-binding family protein contains a A...    27   9.5  
At1g70140.1 68414.m08071 formin homology 2 domain-containing pro...    27   9.5  

>At1g23890.2 68414.m03013 NHL repeat-containing protein contains
           Pfam profile PF01436: NHL repeat
          Length = 400

 Score = 35.1 bits (77), Expect = 0.036
 Identities = 17/55 (30%), Positives = 23/55 (41%)
 Frame = +2

Query: 323 GSGEFDLPAGITTDTYGRIIVIDKDNHRVQIFTSSGNFILKFGSFGKECGQFQYP 487
           G+  F  P G   D  G + V DK N  ++  +SSG+     G   K  G    P
Sbjct: 96  GNSRFSKPRGFAVDAKGNVYVADKSNKAIRKISSSGSVTTIAGGISKAFGHRDGP 150


>At1g23890.1 68414.m03014 NHL repeat-containing protein contains
           Pfam profile PF01436: NHL repeat
          Length = 261

 Score = 35.1 bits (77), Expect = 0.036
 Identities = 17/55 (30%), Positives = 23/55 (41%)
 Frame = +2

Query: 323 GSGEFDLPAGITTDTYGRIIVIDKDNHRVQIFTSSGNFILKFGSFGKECGQFQYP 487
           G+  F  P G   D  G + V DK N  ++  +SSG+     G   K  G    P
Sbjct: 96  GNSRFSKPRGFAVDAKGNVYVADKSNKAIRKISSSGSVTTIAGGISKAFGHRDGP 150


>At1g56500.1 68414.m06497 haloacid dehalogenase-like hydrolase
           family protein low similarity to SP|P95649 CbbY protein
           {Rhodobacter sphaeroides}; contains InterPro accession
           IPR005834: Haloacid dehalogenase-like hydrolase
          Length = 1055

 Score = 31.9 bits (69), Expect = 0.33
 Identities = 14/42 (33%), Positives = 25/42 (59%), Gaps = 1/42 (2%)
 Frame = +2

Query: 344 PAGITTDTYG-RIIVIDKDNHRVQIFTSSGNFILKFGSFGKE 466
           P  +  DT   R+ + D +++R+ +    GNFI++ GS G+E
Sbjct: 569 PGKLAIDTLNNRLFISDSNHNRIIVTDLEGNFIVQIGSSGEE 610


>At5g65130.1 68418.m08193 AP2 domain-containing transcription
           factor, putative similar to AP2 domain transcription
           factor
          Length = 277

 Score = 29.9 bits (64), Expect = 1.3
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -3

Query: 378 MRPYVSVVMPAGRSNSPEPVPLDPNIILNS 289
           + P++ V   +  SNS  P PL PN I N+
Sbjct: 16  LEPFMKVTSSSSTSNSSNPKPLTPNFIPNN 45


>At1g23880.1 68414.m03012 NHL repeat-containing protein contains
           Pfam profile PF01436: NHL repeat
          Length = 545

 Score = 26.6 bits (56), Expect(2) = 2.2
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +3

Query: 186 EGQVSRPWGLCVDKEGNIIVAD 251
           + +++ P GL VD  GNI VAD
Sbjct: 201 DARLNNPKGLTVDDRGNIYVAD 222



 Score = 21.0 bits (42), Expect(2) = 2.2
 Identities = 9/32 (28%), Positives = 17/32 (53%)
 Frame = +3

Query: 9   TRSTNYYDHSISSSMALEPNVSRGIGQAIPGY 104
           ++++N Y  S S S+   P +  G  +  PG+
Sbjct: 163 SQNSNIYQISSSLSLYSRPRLVTGSPEGYPGH 194


>At5g14890.1 68418.m01746 NHL repeat-containing protein contains
           Pfam profile PF01436: NHL repeat
          Length = 754

 Score = 29.1 bits (62), Expect = 2.4
 Identities = 12/22 (54%), Positives = 16/22 (72%)
 Frame = +3

Query: 186 EGQVSRPWGLCVDKEGNIIVAD 251
           E +++RP GL +D  GNI VAD
Sbjct: 148 EARMNRPRGLAMDDRGNIYVAD 169


>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
           SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
           (NR2) {Arabidopsis thaliana}
          Length = 917

 Score = 28.7 bits (61), Expect = 3.1
 Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 6/39 (15%)
 Frame = -2

Query: 541 GYACPSQLYYLTVHGHVP------WILELPAFLPKRTEF 443
           G+  P  L+Y+  HGHVP      W +E+  F+ +  +F
Sbjct: 133 GFITPVPLHYVRNHGHVPKAQWAEWTVEVTGFVKRPMKF 171


>At5g01840.1 68418.m00103 ovate family protein 59% similar to ovate
           protein (GI:23429649) [Lycopersicon esculentum];
           contains TIGRFAM TIGR01568 : uncharacterized
           plant-specific domain TIGR01568
          Length = 270

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = +3

Query: 312 PRAPARVSSICPPASPPTRTDASSLSTKITTEFRSS 419
           PR P+  SS  PP+ PP ++  + L  + T + +SS
Sbjct: 62  PRRPSH-SSKAPPSHPPRKSSGNRLRHRATVDSKSS 96


>At3g56690.1 68416.m06306 calmodulin-binding protein identical to
           calmodulin-binding protein GI:6760428 from [Arabidopsis
           thaliana]
          Length = 1022

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 15/29 (51%), Positives = 21/29 (72%)
 Frame = +3

Query: 315 RAPARVSSICPPASPPTRTDASSLSTKIT 401
           R P+R+S+  PPASP  RT ASS +++ T
Sbjct: 9   RTPSRLSNSEPPASP--RTPASSTTSRDT 35


>At3g50810.1 68416.m05564 integral membrane protein, putative
           contains 4 transmembrane domains; contains Pfam PF04535
           : Domain of unknown function (DUF588);  similar to
           putative ethylene responsive element binding protein
           (GI:22135858) [Arabidopsis thaliana]
          Length = 235

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = +2

Query: 359 TDTYGRIIVIDKDNHRVQIFTSSGNFILKFGSFGKEC 469
           TDTY  ++ +     RV     +G+F+L F S G  C
Sbjct: 63  TDTYSVLVKLLPQELRVLSIVFAGDFVLSFLSLGGAC 99


>At1g64500.1 68414.m07312 glutaredoxin family protein
          Length = 368

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 3/58 (5%)
 Frame = +3

Query: 321 PARVSSICPPASPPTRTDASSLS--TKITTEFRSSRRLVTSY*NS-VRLGRNAGSSSI 485
           P  V S+ PPA+PP R    S S  T    E R+ R +      + V+L R    S +
Sbjct: 93  PPEVDSVKPPATPPRRLPRKSASFHTLDELEVRAKRSIAAQIPTTMVKLKRTESMSKL 150


>At1g10390.1 68414.m01171 nucleoporin family protein contains Pfam
           profiles: PF04096 nucleoporin autopeptidase, PF03093
           nucleoporin FG repeat family
          Length = 1041

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 25/68 (36%), Positives = 31/68 (45%), Gaps = 5/68 (7%)
 Frame = +3

Query: 306 SDPRAPARVSSICPPASPPTRTDASSLSTKITTEFRSSRRLVTSY*NSVRLGRNA----- 470
           ++P  P   S+   P +P T T ASS     T+ F SS   VTS  N    G +      
Sbjct: 432 ANPTNPFSSSTSTNPFAPQTPTIASSSFGTATSNFGSSPFGVTSSSNLFGSGSSTTTSVF 491

Query: 471 GSSSIHGT 494
           GSSS  GT
Sbjct: 492 GSSSAFGT 499


>At4g24610.1 68417.m03525 expressed protein 
          Length = 1145

 Score = 27.1 bits (57), Expect = 9.5
 Identities = 13/40 (32%), Positives = 21/40 (52%)
 Frame = -3

Query: 594  ENELRDVSSVTREQLDAVVTRVRHNYIT*LFTATSHGYWN 475
            E+++R   +  +EQL   V  +     T +F A S GYW+
Sbjct: 1028 ESDIRSKMNNLKEQLTNTVNHLHSVCETHVFIALSRGYWD 1067


>At4g00200.1 68417.m00021 DNA-binding family protein contains a AT
           hook motif (DNA binding motifs with a preference for A/T
           rich regions), Pfam:PF02178
          Length = 345

 Score = 27.1 bits (57), Expect = 9.5
 Identities = 17/53 (32%), Positives = 25/53 (47%)
 Frame = +3

Query: 318 APARVSSICPPASPPTRTDASSLSTKITTEFRSSRRLVTSY*NSVRLGRNAGS 476
           APA V S+ PP SPP    +    T    E   S   ++S+ N   + RN+ +
Sbjct: 284 APAAVMSVPPPPSPPPPAASVFSPTNPDREQPPSSFGISSWTNGQDMPRNSAT 336


>At1g70140.1 68414.m08071 formin homology 2 domain-containing
           protein / FH2 domain-containing protein contains formin
           homology 2 domain, Pfam:PF02181
          Length = 760

 Score = 27.1 bits (57), Expect = 9.5
 Identities = 12/33 (36%), Positives = 16/33 (48%)
 Frame = +3

Query: 309 DPRAPARVSSICPPASPPTRTDASSLSTKITTE 407
           D   P RV +  PP  PP  T A+  +T +  E
Sbjct: 108 DRVGPVRVENTLPPYPPPPMTSAAVTTTTLARE 140


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,888,810
Number of Sequences: 28952
Number of extensions: 303825
Number of successful extensions: 1067
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1016
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1067
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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