BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0250 (715 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|... 150 2e-37 SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 30 0.38 SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase Trp4|S... 26 4.7 SPBC1709.16c |||aromatic ring-opening dioxygenase |Schizosacchar... 26 6.1 SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 25 8.1 >SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 150 bits (363), Expect = 2e-37 Identities = 72/148 (48%), Positives = 100/148 (67%) Frame = +2 Query: 242 CGLFFPMLKIADFLKAGCEVTILFADLHAYLDNMKAPWELLALRTQYYEAAIKAMLTSIG 421 CG F PM+K+ADFLKAG VT+L AD+HA+LDNMKAP EL+ R +YYE +K++L S+ Sbjct: 48 CGYFVPMMKLADFLKAGVHVTVLLADVHAFLDNMKAPMELVQHRVRYYEIIVKSILKSLN 107 Query: 422 VPLEKLKFVRGTEYQLSKEYTLDVYRLSSL*QNMMLRKPEQKL*NK*NIHYLVDFSIQTS 601 +P+EKL+FV G+ YQL ++YTLD +RL++ R+ ++ + L Sbjct: 108 IPIEKLRFVIGSSYQLKEDYTLDNFRLAASTTEHTARRAGAEVVKQVASPLLSSLIYPGM 167 Query: 602 KLWDEEYLKVDAQFGGVDPRKIFTMSEK 685 + DE+YL VD QFGGVD RKIF M+E+ Sbjct: 168 QALDEQYLGVDVQFGGVDQRKIFVMAEE 195 Score = 68.1 bits (159), Expect = 1e-12 Identities = 30/48 (62%), Positives = 35/48 (72%) Frame = +3 Query: 111 TWQEKKHLITRNLQEVLGDDKLTEILKQRDLKIYWGTATTGRPHVAYF 254 T EK LITRNLQEVLG + EIL +RD+ +YWG+A TGRPH YF Sbjct: 4 TPDEKYELITRNLQEVLGAKMIREILNERDISLYWGSAPTGRPHCGYF 51 >SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 787 Score = 29.9 bits (64), Expect = 0.38 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = +2 Query: 356 ELLALRTQYYEAAIKAMLTSIGVPLEKLKFVRGTEYQ 466 ELL L T+ E + A T G+P ++ ++G Y+ Sbjct: 161 ELLTLATELAERLLPAFRTPTGIPFARINLMKGVAYR 197 >SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase Trp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 354 Score = 26.2 bits (55), Expect = 4.7 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +2 Query: 365 ALRTQYYEAAIKAMLTSIGVPLEKLKFVRGTEYQLSKE 478 A+ + YEAA++A+LT P+E F+ ++E Sbjct: 22 AIPLENYEAALRAILTGKASPVETASFLASLHLTKAEE 59 >SPBC1709.16c |||aromatic ring-opening dioxygenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 285 Score = 25.8 bits (54), Expect = 6.1 Identities = 19/75 (25%), Positives = 28/75 (37%) Frame = -2 Query: 621 YSSSQSLEVWIEKSTK*WMFYLFYNFCSGFLSIMFCYNDDNL*TSNVYSLLSWYSVPRTN 442 Y S S+EV+ + + +FY +Y F I F + S L Y +P Sbjct: 71 YESRGSVEVY-SRDDENPLFYDYYGFPDYLYQIKFHSKGSKRIADQIISALKEYQIPAKT 129 Query: 441 FSFSSGTPIDVSIAF 397 S G V + F Sbjct: 130 VSGDRGLDHGVFVPF 144 >SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1191 Score = 25.4 bits (53), Expect = 8.1 Identities = 9/26 (34%), Positives = 19/26 (73%) Frame = -3 Query: 530 LASCSVTTMIIYRHLMYTLY*AGTLY 453 +++C +T +++Y++ M+TL G LY Sbjct: 546 VSNCPITYVMVYKNEMWTLDDMGKLY 571 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,840,280 Number of Sequences: 5004 Number of extensions: 58264 Number of successful extensions: 152 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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