SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0250
         (715 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|...   150   2e-37
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar...    30   0.38 
SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase Trp4|S...    26   4.7  
SPBC1709.16c |||aromatic ring-opening dioxygenase |Schizosacchar...    26   6.1  
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar...    25   8.1  

>SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 401

 Score =  150 bits (363), Expect = 2e-37
 Identities = 72/148 (48%), Positives = 100/148 (67%)
 Frame = +2

Query: 242 CGLFFPMLKIADFLKAGCEVTILFADLHAYLDNMKAPWELLALRTQYYEAAIKAMLTSIG 421
           CG F PM+K+ADFLKAG  VT+L AD+HA+LDNMKAP EL+  R +YYE  +K++L S+ 
Sbjct: 48  CGYFVPMMKLADFLKAGVHVTVLLADVHAFLDNMKAPMELVQHRVRYYEIIVKSILKSLN 107

Query: 422 VPLEKLKFVRGTEYQLSKEYTLDVYRLSSL*QNMMLRKPEQKL*NK*NIHYLVDFSIQTS 601
           +P+EKL+FV G+ YQL ++YTLD +RL++       R+   ++  +     L        
Sbjct: 108 IPIEKLRFVIGSSYQLKEDYTLDNFRLAASTTEHTARRAGAEVVKQVASPLLSSLIYPGM 167

Query: 602 KLWDEEYLKVDAQFGGVDPRKIFTMSEK 685
           +  DE+YL VD QFGGVD RKIF M+E+
Sbjct: 168 QALDEQYLGVDVQFGGVDQRKIFVMAEE 195



 Score = 68.1 bits (159), Expect = 1e-12
 Identities = 30/48 (62%), Positives = 35/48 (72%)
 Frame = +3

Query: 111 TWQEKKHLITRNLQEVLGDDKLTEILKQRDLKIYWGTATTGRPHVAYF 254
           T  EK  LITRNLQEVLG   + EIL +RD+ +YWG+A TGRPH  YF
Sbjct: 4   TPDEKYELITRNLQEVLGAKMIREILNERDISLYWGSAPTGRPHCGYF 51


>SPAC23A1.04c |mnl1||alpha mannosidase-like
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score = 29.9 bits (64), Expect = 0.38
 Identities = 12/37 (32%), Positives = 20/37 (54%)
 Frame = +2

Query: 356 ELLALRTQYYEAAIKAMLTSIGVPLEKLKFVRGTEYQ 466
           ELL L T+  E  + A  T  G+P  ++  ++G  Y+
Sbjct: 161 ELLTLATELAERLLPAFRTPTGIPFARINLMKGVAYR 197


>SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase
           Trp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 354

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +2

Query: 365 ALRTQYYEAAIKAMLTSIGVPLEKLKFVRGTEYQLSKE 478
           A+  + YEAA++A+LT    P+E   F+       ++E
Sbjct: 22  AIPLENYEAALRAILTGKASPVETASFLASLHLTKAEE 59


>SPBC1709.16c |||aromatic ring-opening dioxygenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 285

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 19/75 (25%), Positives = 28/75 (37%)
 Frame = -2

Query: 621 YSSSQSLEVWIEKSTK*WMFYLFYNFCSGFLSIMFCYNDDNL*TSNVYSLLSWYSVPRTN 442
           Y S  S+EV+  +  +  +FY +Y F      I F           + S L  Y +P   
Sbjct: 71  YESRGSVEVY-SRDDENPLFYDYYGFPDYLYQIKFHSKGSKRIADQIISALKEYQIPAKT 129

Query: 441 FSFSSGTPIDVSIAF 397
            S   G    V + F
Sbjct: 130 VSGDRGLDHGVFVPF 144


>SPAC9G1.10c |||inositol polyphosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1191

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 9/26 (34%), Positives = 19/26 (73%)
 Frame = -3

Query: 530 LASCSVTTMIIYRHLMYTLY*AGTLY 453
           +++C +T +++Y++ M+TL   G LY
Sbjct: 546 VSNCPITYVMVYKNEMWTLDDMGKLY 571


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,840,280
Number of Sequences: 5004
Number of extensions: 58264
Number of successful extensions: 152
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -