Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0249
(813 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g13700.1 68417.m02128 serine/threonine protein phosphatase fa... 57 2e-08
At3g07130.1 68416.m00849 serine/threonine protein phosphatase fa... 56 4e-08
At3g20500.1 68416.m02596 calcineurin-like phosphoesterase family... 48 6e-06
At3g52820.1 68416.m05820 purple acid phosphatase (PAP22) identic... 47 2e-05
At3g52810.1 68416.m05819 purple acid phosphatase (PAP21) identic... 46 4e-05
At2g32770.3 68415.m04011 purple acid phosphatase (PAP13) identic... 45 7e-05
At2g32770.2 68415.m04012 purple acid phosphatase (PAP13) identic... 45 7e-05
At1g13900.1 68414.m01631 calcineurin-like phosphoesterase family... 45 7e-05
At2g03450.1 68415.m00303 purple acid phosphatase (PAP9) identica... 44 9e-05
At5g50400.1 68418.m06242 calcineurin-like phosphoesterase family... 40 0.002
At3g52780.2 68416.m05816 purple acid phosphatase (PAP20) identic... 39 0.003
At3g52780.1 68416.m05815 purple acid phosphatase (PAP20) identic... 39 0.003
At4g24890.1 68417.m03562 calcineurin-like phosphoesterase family... 35 0.074
At1g13750.1 68414.m01614 calcineurin-like phosphoesterase family... 33 0.23
At2g16430.2 68415.m01882 purple acid phosphatase (PAP10) identic... 31 0.69
At2g16430.1 68415.m01881 purple acid phosphatase (PAP10) identic... 31 0.69
At2g09970.1 68415.m01036 hypothetical protein 28 6.4
At2g27190.1 68415.m03268 iron(III)-zinc(II) purple acid phosphat... 28 8.5
>At4g13700.1 68417.m02128 serine/threonine protein phosphatase
family protein contains Pfam domain PF00149: Ser/Thr
protein phosphatase
Length = 474
Score = 56.8 bits (131), Expect = 2e-08
Identities = 37/89 (41%), Positives = 49/89 (55%), Gaps = 13/89 (14%)
Frame = +2
Query: 263 VPYMTCPGNHEEK-----YNFSNYVNRFSMP----GPDSNLYYSFDLGPVHFVSVSTEVY 415
VP M GNHE + F +Y RF++P G +SNLYYSFD G VHFV + V
Sbjct: 276 VPTMVIEGNHEIEPQASGITFKSYSERFAVPASESGSNSNLYYSFDAGGVHFVMLGAYVD 335
Query: 416 Y----FTEYGLKLIVNQYDWLKEDLAEAN 490
Y + L++ QY WLKEDL++ +
Sbjct: 336 YNNTGKSMDTLEVSWLQYAWLKEDLSKVD 364
>At3g07130.1 68416.m00849 serine/threonine protein phosphatase
family protein contains similarity to purple acid
phosphatase [Arabidopsis thaliana]
gi|20257489|gb|AAM15914
Length = 532
Score = 55.6 bits (128), Expect = 4e-08
Identities = 33/85 (38%), Positives = 43/85 (50%), Gaps = 9/85 (10%)
Frame = +2
Query: 263 VPYMTCPGNHE-----EKYNFSNYVNRFSMP----GPDSNLYYSFDLGPVHFVSVSTEVY 415
VP M GNHE E F Y +RF+ P G S LYYSF+ G +HFV + +
Sbjct: 269 VPLMVIEGNHEIELQAENKTFEAYSSRFAFPFNESGSSSTLYYSFNAGGIHFVMLGAYIA 328
Query: 416 YFTEYGLKLIVNQYDWLKEDLAEAN 490
Y QY+WLK+DLA+ +
Sbjct: 329 YDKS------AEQYEWLKKDLAKVD 347
>At3g20500.1 68416.m02596 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 437
Score = 48.4 bits (110), Expect = 6e-06
Identities = 30/89 (33%), Positives = 50/89 (56%), Gaps = 10/89 (11%)
Frame = +2
Query: 254 AATVPYMTCPGNHEEKY------NFSNYVNRFSMP----GPDSNLYYSFDLGPVHFVSVS 403
A+ P+M GNHE++ F ++ +R+ MP G +SNLYYSF++ VH + +
Sbjct: 197 ASVRPWMVTQGNHEKESIPFIVDEFVSFNSRWKMPYEESGSNSNLYYSFEVAGVHAIMLG 256
Query: 404 TEVYYFTEYGLKLIVNQYDWLKEDLAEAN 490
+ +T+Y +QY WLK DL++ +
Sbjct: 257 S----YTDY--DRYSDQYSWLKADLSKVD 279
Score = 30.7 bits (66), Expect = 1.2
Identities = 20/53 (37%), Positives = 26/53 (49%)
Frame = +1
Query: 508 KRPWIILFGHRPMYCSNSNDIDCSVELTRVGIAGMFGLEPLLIEFGVDVVIWG 666
+ PW+I+ H P Y NSN+ G M +EPLL GVD+V G
Sbjct: 282 RTPWLIVLFHVPWY--NSNNAH-----QHEGDEMMAEMEPLLYASGVDIVFTG 327
>At3g52820.1 68416.m05820 purple acid phosphatase (PAP22) identical
to purple acid phosphatase (PAP22)GI:20257494 from
[Arabidopsis thaliana]
Length = 434
Score = 46.8 bits (106), Expect = 2e-05
Identities = 32/90 (35%), Positives = 48/90 (53%), Gaps = 11/90 (12%)
Frame = +2
Query: 254 AATVPYMTCPGNHE-------EKYNFSNYVNRFSMPGPDS----NLYYSFDLGPVHFVSV 400
A+ P+M GNHE E F +Y R+ MP +S NLYYSFD+ VH V +
Sbjct: 197 ASKRPWMVTEGNHEIEFFPIIEHTTFKSYNARWLMPHTESFSTSNLYYSFDVAGVHTVML 256
Query: 401 STEVYYFTEYGLKLIVNQYDWLKEDLAEAN 490
+ +T++ + +QY WL+ DLA+ +
Sbjct: 257 GS----YTDFDCE--SDQYQWLQADLAKVD 280
>At3g52810.1 68416.m05819 purple acid phosphatase (PAP21) identical
to purple acid phosphatase GI:20257492 from [Arabidopsis
thaliana]; contains Pfam profile PF00149: Ser/Thr
protein phosphatase
Length = 437
Score = 45.6 bits (103), Expect = 4e-05
Identities = 32/90 (35%), Positives = 48/90 (53%), Gaps = 11/90 (12%)
Frame = +2
Query: 254 AATVPYMTCPGNHE-------EKYNFSNYVNRFSMPGPDS----NLYYSFDLGPVHFVSV 400
A+T P+M GNHE + +F +Y R+ MP +S NLYYSFD+ VH V +
Sbjct: 201 ASTRPWMVTEGNHEIESFPTNDHISFKSYNARWLMPHAESLSHSNLYYSFDVAGVHTVML 260
Query: 401 STEVYYFTEYGLKLIVNQYDWLKEDLAEAN 490
+ +T Y + +QY WL+ DL + +
Sbjct: 261 GS----YTPY--ESHSDQYHWLQADLRKVD 284
Score = 36.3 bits (80), Expect = 0.024
Identities = 29/83 (34%), Positives = 42/83 (50%)
Frame = +3
Query: 21 GWSELFSFKTPPEGEDWLLRAAIYGDMGNKNAHSLSYLQDEAERDHFDVILHVGDFAYDM 200
G + FSFKTPP + + A+ GD+G + ++ L +RD FDV L GD +Y
Sbjct: 129 GHGDEFSFKTPP--SKFPIEFAVAGDLGQTD-WTVRTLDQIRKRD-FDVFLLPGDLSY-A 183
Query: 201 DTEDARVGDEFMRQIQPLPPLCP 269
DT + D F R ++ L P
Sbjct: 184 DTHQP-LWDSFGRLLETLASTRP 205
>At2g32770.3 68415.m04011 purple acid phosphatase (PAP13) identical
to purple acid phosphatase (PAP13) [Arabidopsis
thaliana] GI:20257489; contains Pfam profile PF00149:
Ser/Thr protein phosphatase
Length = 545
Score = 44.8 bits (101), Expect = 7e-05
Identities = 30/91 (32%), Positives = 42/91 (46%), Gaps = 10/91 (10%)
Frame = +2
Query: 257 ATVPYMTCPGNHE------EKYNFSNYVNRFSMPGPDSN----LYYSFDLGPVHFVSVST 406
A VP M G HE F+ Y +RF+ P +S LYYSF+ G HF+ +++
Sbjct: 286 ANVPTMMVAGEHEIEPQTENNLTFAAYSSRFAFPSNESGSFSPLYYSFNAGGAHFIVLNS 345
Query: 407 EVYYFTEYGLKLIVNQYDWLKEDLAEANTPE 499
Y +QY WL+ DL + N E
Sbjct: 346 YTLYDNS------SDQYIWLESDLIKINRSE 370
>At2g32770.2 68415.m04012 purple acid phosphatase (PAP13) identical
to purple acid phosphatase (PAP13) [Arabidopsis
thaliana] GI:20257489; contains Pfam profile PF00149:
Ser/Thr protein phosphatase
Length = 428
Score = 44.8 bits (101), Expect = 7e-05
Identities = 30/91 (32%), Positives = 42/91 (46%), Gaps = 10/91 (10%)
Frame = +2
Query: 257 ATVPYMTCPGNHE------EKYNFSNYVNRFSMPGPDSN----LYYSFDLGPVHFVSVST 406
A VP M G HE F+ Y +RF+ P +S LYYSF+ G HF+ +++
Sbjct: 205 ANVPTMMVAGEHEIEPQTENNLTFAAYSSRFAFPSNESGSFSPLYYSFNAGGAHFIVLNS 264
Query: 407 EVYYFTEYGLKLIVNQYDWLKEDLAEANTPE 499
Y +QY WL+ DL + N E
Sbjct: 265 YTLYDNS------SDQYIWLESDLIKINRSE 289
>At1g13900.1 68414.m01631 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 656
Score = 44.8 bits (101), Expect = 7e-05
Identities = 38/107 (35%), Positives = 52/107 (48%), Gaps = 32/107 (29%)
Frame = +2
Query: 254 AATVPYMTCPGNHEEKYN------------FSN---------YVNRFSMPG--------- 343
A+TVPY C GNHE ++ + N Y +F+MPG
Sbjct: 328 ASTVPYHVCIGNHEYDFSTQPWKPDWAASIYGNDGGGECGVPYSLKFNMPGNSSESTGMK 387
Query: 344 --PDSNLYYSFDLGPVHFVSVSTEVYYFTEYGLKLIVNQYDWLKEDL 478
P NLYYS+D+G VHFV +STE F + G +QY+++K DL
Sbjct: 388 APPTRNLYYSYDMGTVHFVYISTET-NFLKGG-----SQYEFIKRDL 428
Score = 36.7 bits (81), Expect = 0.018
Identities = 21/83 (25%), Positives = 37/83 (44%)
Frame = +1
Query: 508 KRPWIILFGHRPMYCSNSNDIDCSVELTRVGIAGMFGLEPLLIEFGVDVVIWGS*ALLRE 687
K P++++ GHRPMY +++ D + V LEPL ++ V + +WG
Sbjct: 435 KTPFVVVQGHRPMYTTSNEVRDTMIRQKMVE-----HLEPLFVKNNVTLALWGHVHRYER 489
Query: 688 ILALVTIMLCTMVPRGPLHPILG 756
+ T P+H ++G
Sbjct: 490 FCPISNNTCGTQWQGNPVHLVIG 512
>At2g03450.1 68415.m00303 purple acid phosphatase (PAP9) identical
to purple acid phosphatase [Arabidopsis thaliana]
GI:20257481; contains Pfam profile: PF00149
calcineurin-like phosphoesterase; contains
metallo-phosphoesterase motif (PS50185)
Length = 651
Score = 44.4 bits (100), Expect = 9e-05
Identities = 26/51 (50%), Positives = 31/51 (60%), Gaps = 1/51 (1%)
Frame = +2
Query: 341 GPDS-NLYYSFDLGPVHFVSVSTEVYYFTEYGLKLIVNQYDWLKEDLAEAN 490
GP S NLYYS+D+G VHFV +STE F + G QY +LK DL N
Sbjct: 388 GPQSRNLYYSYDMGSVHFVYISTET-DFLKGG-----KQYSFLKSDLESVN 432
Score = 38.3 bits (85), Expect = 0.006
Identities = 22/83 (26%), Positives = 38/83 (45%)
Frame = +1
Query: 508 KRPWIILFGHRPMYCSNSNDIDCSVELTRVGIAGMFGLEPLLIEFGVDVVIWGS*ALLRE 687
K P++++ GHRPMY ++ D ++ + LEPLL++ V V +WG
Sbjct: 435 KTPFVVVQGHRPMYTTSRKIRDAAIREKMIE-----HLEPLLVKNNVTVALWGHVHRYER 489
Query: 688 ILALVTIMLCTMVPRGPLHPILG 756
A+ P+H ++G
Sbjct: 490 FCAISNNTCGERWQGNPVHLVIG 512
>At5g50400.1 68418.m06242 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 611
Score = 39.9 bits (89), Expect = 0.002
Identities = 31/100 (31%), Positives = 48/100 (48%), Gaps = 13/100 (13%)
Frame = +3
Query: 9 GSVYGWSELFSFKTPP-EGEDWLLRAAIYGDMG-NKNAHSLSY---------LQDEAERD 155
GS+ WS+ F+FK+ P G+D L R I+GDMG + S Y D+ +D
Sbjct: 264 GSIV-WSKNFTFKSSPYPGQDSLQRVIIFGDMGKGERDGSNEYNDYQPGSLNTTDQLIKD 322
Query: 156 --HFDVILHVGDFAYDMDTEDARVGDEFMRQIQPLPPLCP 269
+ D++ H+GD Y D+F Q++P+ P
Sbjct: 323 LKNIDIVFHIGDITY--ANGYISQWDQFTAQVEPIASTVP 360
>At3g52780.2 68416.m05816 purple acid phosphatase (PAP20) identical
to purple acid phosphatase GI:20257491 from [Arabidopsis
thaliana]
Length = 361
Score = 39.1 bits (87), Expect = 0.003
Identities = 32/86 (37%), Positives = 44/86 (51%), Gaps = 11/86 (12%)
Frame = +2
Query: 254 AATVPYMTCPGNHE-EKYN------FSNYVNRFSMP----GPDSNLYYSFDLGPVHFVSV 400
A+ P+M GNHE EK F+ Y R+ MP G SNLYYSF++ VH + +
Sbjct: 196 ASQRPWMVTHGNHELEKIPILHSNPFTAYNKRWRMPFEESGSSSNLYYSFNVYGVHIIML 255
Query: 401 STEVYYFTEYGLKLIVNQYDWLKEDL 478
+ Y E G + QY WL+ +L
Sbjct: 256 GS--YTDFEPGSE----QYQWLENNL 275
>At3g52780.1 68416.m05815 purple acid phosphatase (PAP20) identical
to purple acid phosphatase GI:20257491 from [Arabidopsis
thaliana]
Length = 427
Score = 39.1 bits (87), Expect = 0.003
Identities = 32/86 (37%), Positives = 44/86 (51%), Gaps = 11/86 (12%)
Frame = +2
Query: 254 AATVPYMTCPGNHE-EKYN------FSNYVNRFSMP----GPDSNLYYSFDLGPVHFVSV 400
A+ P+M GNHE EK F+ Y R+ MP G SNLYYSF++ VH + +
Sbjct: 196 ASQRPWMVTHGNHELEKIPILHSNPFTAYNKRWRMPFEESGSSSNLYYSFNVYGVHIIML 255
Query: 401 STEVYYFTEYGLKLIVNQYDWLKEDL 478
+ Y E G + QY WL+ +L
Sbjct: 256 GS--YTDFEPGSE----QYQWLENNL 275
>At4g24890.1 68417.m03562 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 615
Score = 34.7 bits (76), Expect = 0.074
Identities = 27/95 (28%), Positives = 42/95 (44%), Gaps = 13/95 (13%)
Frame = +3
Query: 24 WSELFSFKTPP-EGEDWLLRAAIYGDMG-NKNAHSLSY---------LQDEAERD--HFD 164
WS+ ++F + P G+D R I+GDMG + S Y D+ +D D
Sbjct: 272 WSKNYTFVSSPYPGQDSKQRVIIFGDMGKGERDGSNEYNDYQPGSLNTTDQVIKDLKDID 331
Query: 165 VILHVGDFAYDMDTEDARVGDEFMRQIQPLPPLCP 269
++ H+GD Y D+F Q+QP+ P
Sbjct: 332 IVFHIGDLTY--SNGYLSQWDQFTAQVQPIASTVP 364
>At1g13750.1 68414.m01614 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 613
Score = 33.1 bits (72), Expect = 0.23
Identities = 25/95 (26%), Positives = 41/95 (43%), Gaps = 13/95 (13%)
Frame = +3
Query: 24 WSELFSFKTPP-EGEDWLLRAAIYGDMGNKNAHSLSYLQD----------EAERD--HFD 164
WS+ + FK+ P G++ + + I+GDMG S D + +D D
Sbjct: 270 WSKEYQFKSSPFPGQNSVQQVVIFGDMGKAEVDGSSEYNDFQRASLNTTKQLIKDLKKTD 329
Query: 165 VILHVGDFAYDMDTEDARVGDEFMRQIQPLPPLCP 269
+ H+GD Y D+F+ QI+P+ P
Sbjct: 330 AVFHIGDICY--ANGYLSQWDQFIAQIEPIASTVP 362
>At2g16430.2 68415.m01882 purple acid phosphatase (PAP10) identical
to purple acid phosphatase (PAP10) GI:20257482 from
[Arabidopsis thaliana]
Length = 468
Score = 31.5 bits (68), Expect = 0.69
Identities = 24/95 (25%), Positives = 41/95 (43%), Gaps = 12/95 (12%)
Frame = +2
Query: 251 AAATVPYMTCPGNHE--------EKYNFSNYVNRFSMP----GPDSNLYYSFDLGPVHFV 394
+ A P++ GNHE E F + +R+ P G +YS GP + +
Sbjct: 221 STAYQPWIWTTGNHELDFAPEIGENRPFKPFTHRYRTPYRSSGSTEPFWYSIKRGPAYII 280
Query: 395 SVSTEVYYFTEYGLKLIVNQYDWLKEDLAEANTPE 499
+++ ++ YG QY WL+E+ + N E
Sbjct: 281 VLAS----YSAYGK--YTPQYQWLEEEFPKVNRTE 309
Score = 28.7 bits (61), Expect = 4.9
Identities = 17/54 (31%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = +3
Query: 36 FSFKTPPE-GEDWLLRAAIYGDMGNKNAHSLSYLQDEAERDHFDVILHVGDFAY 194
F F TPPE G D + GD+G +++ E +L VGD +Y
Sbjct: 146 FWFFTPPEIGPDVPYTFGLIGDLGQSYDSNITLTHYENNPTKGQAVLFVGDISY 199
>At2g16430.1 68415.m01881 purple acid phosphatase (PAP10) identical
to purple acid phosphatase (PAP10) GI:20257482 from
[Arabidopsis thaliana]
Length = 348
Score = 31.5 bits (68), Expect = 0.69
Identities = 24/95 (25%), Positives = 41/95 (43%), Gaps = 12/95 (12%)
Frame = +2
Query: 251 AAATVPYMTCPGNHE--------EKYNFSNYVNRFSMP----GPDSNLYYSFDLGPVHFV 394
+ A P++ GNHE E F + +R+ P G +YS GP + +
Sbjct: 101 STAYQPWIWTTGNHELDFAPEIGENRPFKPFTHRYRTPYRSSGSTEPFWYSIKRGPAYII 160
Query: 395 SVSTEVYYFTEYGLKLIVNQYDWLKEDLAEANTPE 499
+++ ++ YG QY WL+E+ + N E
Sbjct: 161 VLAS----YSAYGK--YTPQYQWLEEEFPKVNRTE 189
Score = 28.7 bits (61), Expect = 4.9
Identities = 17/54 (31%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = +3
Query: 36 FSFKTPPE-GEDWLLRAAIYGDMGNKNAHSLSYLQDEAERDHFDVILHVGDFAY 194
F F TPPE G D + GD+G +++ E +L VGD +Y
Sbjct: 26 FWFFTPPEIGPDVPYTFGLIGDLGQSYDSNITLTHYENNPTKGQAVLFVGDISY 79
>At2g09970.1 68415.m01036 hypothetical protein
Length = 166
Score = 28.3 bits (60), Expect = 6.4
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = -2
Query: 734 PLGTIVHNIIVTRARISRKSAHEPHMTTSTPNSISNGSRPN 612
P + ++I +I RKS P M S PNS S + N
Sbjct: 106 PPSNLTRHLISAMRQILRKSLDSPRMLRSMPNSPSKDDQDN 146
>At2g27190.1 68415.m03268 iron(III)-zinc(II) purple acid phosphatase
(PAP12) identical to iron(III)-zinc(II) purple acid
phosphatase [precursor] SP:Q38924 from [Arabidopsis
thaliana]
Length = 469
Score = 27.9 bits (59), Expect = 8.5
Identities = 17/58 (29%), Positives = 24/58 (41%), Gaps = 1/58 (1%)
Frame = +3
Query: 24 WSELFSFKTPPE-GEDWLLRAAIYGDMGNKNAHSLSYLQDEAERDHFDVILHVGDFAY 194
WS F F PP+ G D + GD+G + + E +L VGD +Y
Sbjct: 143 WSRRFWFFIPPKSGPDVPYTFGLIGDLGQTYDSNSTLSHYEMNPGKGQAVLFVGDLSY 200
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,724,142
Number of Sequences: 28952
Number of extensions: 357565
Number of successful extensions: 1177
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1162
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1853336000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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