BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0248 (647 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 80 2e-16 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 79 5e-16 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 75 7e-15 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 74 2e-14 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 70 3e-13 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 67 2e-12 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 62 5e-11 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 62 9e-11 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 61 2e-10 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 60 2e-10 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 54 2e-08 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 53 4e-08 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 44 3e-05 SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 33 0.027 SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 28 1.3 SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 27 3.1 SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|... 26 4.1 SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|ch... 26 4.1 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 26 4.1 SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|... 26 5.4 SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pomb... 25 7.1 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 80.2 bits (189), Expect = 2e-16 Identities = 37/90 (41%), Positives = 53/90 (58%), Gaps = 1/90 (1%) Frame = +1 Query: 1 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFK 177 RL KE +D+R+ + R VD+ N+ W + P + Y G F + FP +YPF+ Sbjct: 11 RLTKEYSDLREHPIPDIRVNLVDD-NLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQ 69 Query: 178 PPKISFKTKIYHPNIDEKGQVCLPIIVQKI 267 PP I F T+IYHPN D +G VCL I+ Q++ Sbjct: 70 PPTIEFTTRIYHPNFDSEGNVCLAILKQQV 99 Score = 45.6 bits (103), Expect = 6e-06 Identities = 24/88 (27%), Positives = 46/88 (52%) Frame = +3 Query: 168 PIQTPEN*FQNKDLPP*H**KGSGVPAHHSAENWKPATKTDQVIQALVALVNDPEPEHPL 347 P Q P F + P +G+ A + +KP+ K V++ ++ L+ +P P+ PL Sbjct: 67 PFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRSVLEQILQLLREPNPDDPL 126 Query: 348 RAELAEEFLKDRKKFTKNAEEFTKKHSE 431 A +AE++ DR F K A ++ ++ ++ Sbjct: 127 VASIAEQYRNDRPSFDKIARDYVEQFAK 154 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 79.0 bits (186), Expect = 5e-16 Identities = 36/86 (41%), Positives = 54/86 (62%), Gaps = 1/86 (1%) Frame = +1 Query: 1 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFK 177 R+ +EL D+ + S V + ++ WQ I+ P + PY G F + I+FP +YPFK Sbjct: 5 RINRELADLGKDPPSSCSAGPVGD-DLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFK 63 Query: 178 PPKISFKTKIYHPNIDEKGQVCLPII 255 PPK++F T+IYHPNI+ G +CL I+ Sbjct: 64 PPKVNFTTRIYHPNINSNGSICLDIL 89 Score = 48.4 bits (110), Expect = 9e-07 Identities = 18/56 (32%), Positives = 35/56 (62%) Frame = +3 Query: 261 ENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEEFTKKHS 428 + W PA +V+ ++ +L+ DP P+ PL E+A + DR ++ +A E+T+K++ Sbjct: 91 DQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHVYKTDRSRYELSAREWTRKYA 146 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 75.4 bits (177), Expect = 7e-15 Identities = 31/85 (36%), Positives = 55/85 (64%), Gaps = 1/85 (1%) Frame = +1 Query: 4 LQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFKP 180 L+K+L +I+++ + F VD+ +I W+ +I+ P++ Y G F ++FP +YP P Sbjct: 10 LRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMP 69 Query: 181 PKISFKTKIYHPNIDEKGQVCLPII 255 PK+ F T+I+HPN+ G+VC+ I+ Sbjct: 70 PKMKFTTEIWHPNVHPNGEVCISIL 94 Score = 36.3 bits (80), Expect = 0.004 Identities = 12/59 (20%), Positives = 31/59 (52%) Frame = +3 Query: 255 SAENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEEFTKKHSE 431 + E W P + ++ +++++++ P E P + A+EF ++ ++F K ++ E Sbjct: 107 AGERWLPVHSPETILISVISMLSSPNDESPANIDAAKEFRENPQEFKKRVRRLVRRSIE 165 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 73.7 bits (173), Expect = 2e-14 Identities = 33/89 (37%), Positives = 49/89 (55%), Gaps = 1/89 (1%) Frame = +1 Query: 1 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIVPDNPPYNKGA-FRIEINFPAEYPFK 177 RL+ EL + S +SN+L W G I + Y +G F+I ++FPA YP+ Sbjct: 32 RLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYS 91 Query: 178 PPKISFKTKIYHPNIDEKGQVCLPIIVQK 264 PP I F + ++HPN+D G +CL I+ K Sbjct: 92 PPTIIFTSPMWHPNVDMSGNICLDILKDK 120 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 70.1 bits (164), Expect = 3e-13 Identities = 33/87 (37%), Positives = 54/87 (62%), Gaps = 2/87 (2%) Frame = +1 Query: 1 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFK 177 R+ KEL D++Q + +++ +I +G+ P+ PY G F ++I P +YPF+ Sbjct: 8 RIAKELADVQQDKQAGIQVWTIND-DISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFR 66 Query: 178 PPKISFKTKIYHPNI-DEKGQVCLPII 255 PPK++F TKIYHPN+ + G +CL I+ Sbjct: 67 PPKMNFDTKIYHPNVSSQTGAICLDIL 93 Score = 39.5 bits (88), Expect = 4e-04 Identities = 15/56 (26%), Positives = 33/56 (58%) Frame = +3 Query: 261 ENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEEFTKKHS 428 + W P + +L +L+ PEP +P A++A+ +L++ ++F + A E+T ++ Sbjct: 95 DQWSPVYTMKSALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQFVRTAREWTSSYA 150 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 66.9 bits (156), Expect = 2e-12 Identities = 28/86 (32%), Positives = 47/86 (54%), Gaps = 1/86 (1%) Frame = +1 Query: 1 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFK 177 RL KE ++ ++G +E + TW LI PD P+ G + + FP++YP Sbjct: 9 RLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLG 68 Query: 178 PPKISFKTKIYHPNIDEKGQVCLPII 255 PP + F+ + +HPN+ + G VC+ I+ Sbjct: 69 PPTLKFECEFFHPNVYKDGTVCISIL 94 Score = 31.1 bits (67), Expect = 0.14 Identities = 9/56 (16%), Positives = 30/56 (53%) Frame = +3 Query: 255 SAENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEEFTKK 422 S+E W P ++++ ++++++ +P E + + + +DR+++ + +K Sbjct: 107 SSERWSPVQSVEKILLSVMSMLAEPNDESGANIDACKMWREDREEYCRVVRRLARK 162 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 62.5 bits (145), Expect = 5e-11 Identities = 25/53 (47%), Positives = 35/53 (66%), Gaps = 1/53 (1%) Frame = +1 Query: 106 PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDE-KGQVCLPIIVQ 261 P PY+ G +++ + P+EYP+K P I F +I+HPNIDE G VCL +I Q Sbjct: 39 PSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQ 91 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 61.7 bits (143), Expect = 9e-11 Identities = 23/47 (48%), Positives = 30/47 (63%) Frame = +1 Query: 106 PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDEKGQVCL 246 P Y G F +E+ P EYP PP + F TKIYHPN+D+ G++CL Sbjct: 41 PQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICL 87 Score = 40.3 bits (90), Expect = 2e-04 Identities = 17/56 (30%), Positives = 35/56 (62%) Frame = +3 Query: 261 ENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEEFTKKHS 428 ++W PA + V+ ++ AL+ P P+ PL ++A+ + ++ + NA E+TKK++ Sbjct: 92 KDWSPALQIRTVLLSIQALMGAPNPDDPLDNDVAKIWKENEPQAIANAREWTKKYA 147 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 60.9 bits (141), Expect = 2e-10 Identities = 21/61 (34%), Positives = 40/61 (65%), Gaps = 1/61 (1%) Frame = +1 Query: 76 NILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDEKGQVCLPI 252 N++ W +I+ P + P+ G F++ ++F +YP KPP + F + ++HPN+ G++CL I Sbjct: 32 NVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDI 91 Query: 253 I 255 + Sbjct: 92 L 92 Score = 31.5 bits (68), Expect = 0.11 Identities = 11/48 (22%), Positives = 25/48 (52%) Frame = +3 Query: 267 WKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEE 410 W P ++ ++ +L+NDP P AE A+ +++K++ + + Sbjct: 96 WSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRK 143 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 60.5 bits (140), Expect = 2e-10 Identities = 33/87 (37%), Positives = 44/87 (50%) Frame = +1 Query: 1 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIVPDNPPYNKGAFRIEINFPAEYPFKP 180 R+QK++ D+ S + N+L + I PD Y G F+ I YP P Sbjct: 27 RIQKDVTDLEIPSTMSTSWPDPIKLNVLHLE--IRPDEGYYKGGKFKFRIQIDDNYPHDP 84 Query: 181 PKISFKTKIYHPNIDEKGQVCLPIIVQ 261 PK+ KIYHPNID +G VCL I+ Q Sbjct: 85 PKVKCLNKIYHPNIDIEGNVCLNILRQ 111 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 53.6 bits (123), Expect = 2e-08 Identities = 28/88 (31%), Positives = 46/88 (52%), Gaps = 3/88 (3%) Frame = +1 Query: 1 RLQKELNDIRQSGLKSFRDIQ-VDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPF 174 RL KE + + ++ V + ++ W+ +I P PY G + ++I+ YP Sbjct: 7 RLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPI 66 Query: 175 KPPKISFKTKIYHPNID-EKGQVCLPII 255 PP + F+TKI HPNI G+VC+ I+ Sbjct: 67 SPPSVYFQTKIVHPNISWTNGEVCMDIL 94 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 52.8 bits (121), Expect = 4e-08 Identities = 22/67 (32%), Positives = 40/67 (59%), Gaps = 2/67 (2%) Frame = +1 Query: 76 NILTWQGLIVPDNPP--YNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDEKGQVCLP 249 +++ W+ + +P P + G +++ + FP EYP +PPK F ++HPN+ G VCL Sbjct: 37 DLMNWK-VGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLS 95 Query: 250 IIVQKIG 270 I+ ++ G Sbjct: 96 ILNEEEG 102 Score = 35.5 bits (78), Expect = 0.007 Identities = 15/56 (26%), Positives = 28/56 (50%) Frame = +3 Query: 261 ENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEEFTKKHS 428 E WKPA Q++ + L++DP P + E F KD+ ++ K ++++ Sbjct: 101 EGWKPAITIKQILLGIQDLLDDPNIASPAQTEAYTMFKKDKVEYEKRVRAQARENA 156 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 43.6 bits (98), Expect = 3e-05 Identities = 24/68 (35%), Positives = 36/68 (52%), Gaps = 1/68 (1%) Frame = +1 Query: 1 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFK 177 RL KE ++++ ++ D + NIL W +I P + PY G + + FP +YPFK Sbjct: 9 RLMKEYLALQKNPVELV-DAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFK 67 Query: 178 PPKISFKT 201 PP I T Sbjct: 68 PPAIRMIT 75 >SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 33.5 bits (73), Expect = 0.027 Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 2/60 (3%) Frame = +1 Query: 67 DESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNID-EKGQV 240 D+ + W I+ P + + + ++I+ A YP PP ++F ++I P +D E G+V Sbjct: 34 DDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKV 93 >SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 128 Score = 27.9 bits (59), Expect = 1.3 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = -3 Query: 231 LFINVRVVNLCFETNFRGFEWVFCW 157 LF+N V +C+ N+ GF + FCW Sbjct: 41 LFLNYVVDIVCYVKNYNGFSF-FCW 64 >SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 2104 Score = 26.6 bits (56), Expect = 3.1 Identities = 14/43 (32%), Positives = 20/43 (46%) Frame = +1 Query: 151 NFPAEYPFKPPKISFKTKIYHPNIDEKGQVCLPIIVQKIGSLL 279 N P+ P P K S K + N D+ + P IV + S+L Sbjct: 1413 NLPSSQPGSPSKRSGKMEALIRNFDQNSSIPDPFIVNQRNSVL 1455 >SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr 3|||Manual Length = 828 Score = 26.2 bits (55), Expect = 4.1 Identities = 15/55 (27%), Positives = 25/55 (45%) Frame = +1 Query: 73 SNILTWQGLIVPDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDEKGQ 237 S++ T + LI P PP K E E F+P + ++ I P +D + + Sbjct: 566 SSMQTIESLIHPRFPPLQKHLSPSEFENTFESRFEPVSLENQSHISPPQVDSQSE 620 >SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 703 Score = 26.2 bits (55), Expect = 4.1 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = +1 Query: 367 NFSKIGRNLQRMQKNLPKNTVKNGLQIKYT 456 NF N+ R K L +T +NG++ KYT Sbjct: 413 NFKTEDTNVLRYWKTLALSTCENGIEDKYT 442 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 26.2 bits (55), Expect = 4.1 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 2/41 (4%) Frame = -1 Query: 206 IFVLKLIFGG--LNGYSAGKLISMRKAPLL*GGLSGTIRPC 90 + VL+++ G LN +S+ KLI PLL G +R C Sbjct: 450 LLVLQMLCGAHVLNKFSSLKLIMTHVIPLLPGSYQDLVRRC 490 >SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 25.8 bits (54), Expect = 5.4 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = +3 Query: 345 LRAELAEEFLKDRKKFTKNAEEFTKKHSE 431 L AEL E++LKD+ K + E K SE Sbjct: 464 LYAELQEQWLKDKSKKARRRAEREKLRSE 492 >SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1389 Score = 25.4 bits (53), Expect = 7.1 Identities = 11/30 (36%), Positives = 21/30 (70%) Frame = +1 Query: 10 KELNDIRQSGLKSFRDIQVDESNILTWQGL 99 K+L++ +++ L + I++DE +L WQGL Sbjct: 50 KQLSESKEAYLDA---IKIDEKAVLAWQGL 76 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,619,856 Number of Sequences: 5004 Number of extensions: 55218 Number of successful extensions: 162 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 159 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 291768710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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