Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0210
(791 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 96 4e-21
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 96 4e-21
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 76 7e-15
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 54 3e-08
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 44 3e-05
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 39 7e-04
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 38 0.001
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 31 0.14
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 31 0.14
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 31 0.14
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 30 0.33
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 27 3.1
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 27 3.1
SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24 famil... 27 4.1
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 27 4.1
SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 26 5.4
SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 9.4
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 9.4
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 25 9.4
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 96.3 bits (229), Expect = 4e-21
Identities = 67/174 (38%), Positives = 89/174 (51%), Gaps = 1/174 (0%)
Frame = +2
Query: 260 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQXEKSEKGFLINLIDSPGHVD 439
RF DTR DEQ+R +TIKSTAIS+F E+ + D+ + P FL+NLIDSPGHVD
Sbjct: 55 RFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEPADGTD----FLVNLIDSPGHVD 110
Query: 440 FSSEVTAALRVTDGALXXXXXXXXXXYKLKQYCVRLLPSASSLFCS*TKWTVLFLSSNLK 619
FSSEVTAALRVTDGAL + + + L K L +
Sbjct: 111 FSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQIS 170
Query: 620 LKN-YTRRSSVL*KMLTSL*PHITMMVGPMGEVRVDPSKGLWFGFGSGLMGWAF 778
+ Y + V+ + + + + +G+ +V P KG F SGL GWAF
Sbjct: 171 QEELYQNFARVVESVNVVISTYYDKV---LGDCQVFPDKGT-VAFASGLHGWAF 220
Score = 68.1 bits (159), Expect = 1e-12
Identities = 31/42 (73%), Positives = 35/42 (83%)
Frame = +3
Query: 99 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 224
MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV K
Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQK 42
Score = 66.5 bits (155), Expect = 4e-12
Identities = 30/57 (52%), Positives = 40/57 (70%)
Frame = +1
Query: 520 QTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYND 690
QTETVLRQA+ ERI+P++ +NK+DR YQ F R+VE+VNV+I+TY D
Sbjct: 138 QTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYD 194
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 96.3 bits (229), Expect = 4e-21
Identities = 67/174 (38%), Positives = 89/174 (51%), Gaps = 1/174 (0%)
Frame = +2
Query: 260 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQXEKSEKGFLINLIDSPGHVD 439
RF DTR DEQ+R +TIKSTAIS+F E+ + D+ + P FL+NLIDSPGHVD
Sbjct: 55 RFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEPADGTD----FLVNLIDSPGHVD 110
Query: 440 FSSEVTAALRVTDGALXXXXXXXXXXYKLKQYCVRLLPSASSLFCS*TKWTVLFLSSNLK 619
FSSEVTAALRVTDGAL + + + L K L +
Sbjct: 111 FSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQIS 170
Query: 620 LKN-YTRRSSVL*KMLTSL*PHITMMVGPMGEVRVDPSKGLWFGFGSGLMGWAF 778
+ Y + V+ + + + + +G+ +V P KG F SGL GWAF
Sbjct: 171 QEELYQNFARVVESVNVVISTYYDKV---LGDCQVFPDKGT-VAFASGLHGWAF 220
Score = 68.1 bits (159), Expect = 1e-12
Identities = 31/42 (73%), Positives = 35/42 (83%)
Frame = +3
Query: 99 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 224
MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV K
Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQK 42
Score = 66.5 bits (155), Expect = 4e-12
Identities = 30/57 (52%), Positives = 40/57 (70%)
Frame = +1
Query: 520 QTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYND 690
QTETVLRQA+ ERI+P++ +NK+DR YQ F R+VE+VNV+I+TY D
Sbjct: 138 QTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYD 194
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 75.8 bits (178), Expect = 7e-15
Identities = 42/75 (56%), Positives = 56/75 (74%)
Frame = +2
Query: 260 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQXEKSEKGFLINLIDSPGHVD 439
RF D R+DE R IT+KS+AIS+FF++ I+ D+ ++ EK +LINLIDSPGHVD
Sbjct: 55 RFLDFREDEITRGITMKSSAISLFFKV-------ISQNDE-KRVEKDYLINLIDSPGHVD 106
Query: 440 FSSEVTAALRVTDGA 484
FSSEV++A R+ DGA
Sbjct: 107 FSSEVSSASRLCDGA 121
Score = 46.4 bits (105), Expect = 5e-06
Identities = 25/55 (45%), Positives = 31/55 (56%)
Frame = +1
Query: 520 QTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATY 684
QT TVLRQA +RIK IL +NKMDR + R+VE VN +I T+
Sbjct: 134 QTITVLRQAWIDRIKVILVINKMDRLITELKLSPIEAHYHLLRLVEQVNAVIGTF 188
Score = 42.7 bits (96), Expect = 6e-05
Identities = 16/35 (45%), Positives = 27/35 (77%)
Frame = +3
Query: 117 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVS 221
+++ + + NIRN +++AHVDHGK+TL DSL++
Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLA 41
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 53.6 bits (123), Expect = 3e-08
Identities = 29/68 (42%), Positives = 41/68 (60%)
Frame = +2
Query: 284 EQDRCITIKSTAISMFFELEEKDLVFITNPDQXEKSEKGFLINLIDSPGHVDFSSEVTAA 463
E+++ ITI+S A +E + N Q EK + IN+ID+PGH+DF+ EV A
Sbjct: 108 EREKGITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERA 165
Query: 464 LRVTDGAL 487
LRV DGA+
Sbjct: 166 LRVLDGAV 173
Score = 33.9 bits (74), Expect = 0.027
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 2/40 (5%)
Frame = +3
Query: 105 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLV 218
N + E DKKR IRN+ + AH+D GK+T T+ ++
Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVL 80
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 43.6 bits (98), Expect = 3e-05
Identities = 26/79 (32%), Positives = 40/79 (50%)
Frame = +2
Query: 251 RKTRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQXEKSEKGFLINLIDSPG 430
R R+TDT E++R ++IKST +++ + K F ID+PG
Sbjct: 174 RSLRYTDTHYLERERVMSIKSTPLTLAVS---------------DMKGKTFAFQCIDTPG 218
Query: 431 HVDFSSEVTAALRVTDGAL 487
HVDF EV A + ++DG +
Sbjct: 219 HVDFVDEVAAPMAISDGVV 237
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/57 (22%), Positives = 25/57 (43%)
Frame = +1
Query: 523 TETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYNDD 693
T +++ AI + +L +NK+DR Y + +++ VN I + D
Sbjct: 250 TTRIIKHAILHDMPIVLVLNKVDRLILELRLPPNDAYHKLRHVIDEVNDNICQISKD 306
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 39.1 bits (87), Expect = 7e-04
Identities = 15/31 (48%), Positives = 23/31 (74%)
Frame = +2
Query: 395 KGFLINLIDSPGHVDFSSEVTAALRVTDGAL 487
+ +L+NLID+PGHVDF +EV +L +G +
Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEGCI 152
Score = 38.7 bits (86), Expect = 0.001
Identities = 16/32 (50%), Positives = 25/32 (78%)
Frame = +3
Query: 123 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLV 218
+RG+ + +RN +VIAH+DHGKSTL+D ++
Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCIL 79
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 38.3 bits (85), Expect = 0.001
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +2
Query: 407 INLIDSPGHVDFSSEVTAALRVTDGAL 487
INLID+PGH DF+ EV ++ V DGA+
Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAV 121
Score = 33.9 bits (74), Expect = 0.027
Identities = 12/23 (52%), Positives = 20/23 (86%)
Frame = +3
Query: 150 NIRNMSVIAHVDHGKSTLTDSLV 218
+IRN+ +IAH+D GK+TLT+ ++
Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKML 49
Score = 28.7 bits (61), Expect = 1.0
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 520 QTETVLRQAIAERIKPILFMNKMDR 594
QT+ V +QA I ++F+NKMDR
Sbjct: 133 QTKVVWKQATKRGIPKVIFVNKMDR 157
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 31.5 bits (68), Expect = 0.14
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +3
Query: 135 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 224
M K++ N+ VI HVD GKST T L+ K
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYK 30
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 31.5 bits (68), Expect = 0.14
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +3
Query: 135 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 224
M K++ N+ VI HVD GKST T L+ K
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYK 30
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 31.5 bits (68), Expect = 0.14
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +3
Query: 135 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 224
M K++ N+ VI HVD GKST T L+ K
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYK 30
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 30.3 bits (65), Expect = 0.33
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +3
Query: 141 KKRNIRNMSVIAHVDHGKSTLTDSL 215
KK ++ N+ I HVDHGK+TLT ++
Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAI 73
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 27.1 bits (57), Expect = 3.1
Identities = 9/17 (52%), Positives = 15/17 (88%)
Frame = +3
Query: 162 MSVIAHVDHGKSTLTDS 212
++++ HVDHGK+TL D+
Sbjct: 174 VTLMGHVDHGKTTLLDA 190
>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 207
Score = 27.1 bits (57), Expect = 3.1
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = -3
Query: 447 EEKSTCPGESIKLIKKPFSLFSXWSGFVMNTKSFSS-SSKNIEMAVDLMVMQRSCSSLRV 271
EE + P + K KK S F+ T +S S++NI+ A+DL+ + S S ++
Sbjct: 66 EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125
>SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 195
Score = 26.6 bits (56), Expect = 4.1
Identities = 14/44 (31%), Positives = 22/44 (50%)
Frame = -1
Query: 155 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQLPYASNGVRFEKG 24
DIPL + P D + RE + RW + +L + Y ++ F G
Sbjct: 102 DIPLRLIQPYDPLSRETVYMRWKELAMLDKTVDYQNHNQSFPFG 145
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 26.6 bits (56), Expect = 4.1
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 3/48 (6%)
Frame = -3
Query: 360 NTKSFSSSSKNIEMAVDLMVMQRSCSSLRVSV---KRVFRLSHQQ*YR 226
+ KSFSS+S NI+ +DL+ + ++S + F+LS ++ YR
Sbjct: 92 SAKSFSSTSSNIDSNLDLLNYDTPLTISKISFLLQQLEFKLSVEEQYR 139
>SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting
endonuclease Cce1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 258
Score = 26.2 bits (55), Expect = 5.4
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -3
Query: 399 PFSLFSXWSGFVMNTK-SFSSSSKNIEMAVDLMVMQR 292
P S +S W+ V+NTK SFS ++M +L+ Q+
Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELIDGQK 203
>SPBC19F5.03 |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 598
Score = 25.4 bits (53), Expect = 9.4
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = -1
Query: 155 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 60
D P + +H D ++E H RW + +LLN++
Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348
>SPCC1235.05c |fft2||fun thirty related protein
Fft2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1284
Score = 25.4 bits (53), Expect = 9.4
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -3
Query: 150 SASCPSSHGSRLP*NSPF*MVYDFCSIK 67
+ASCP SH L + PF + + C IK
Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 25.4 bits (53), Expect = 9.4
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = +2
Query: 518 YKLKQYCVRLLPSASSLFCS*TKWTVLFLSSNLKLKN 628
YKL+ RL +A S+ C WT LF SN+ +N
Sbjct: 356 YKLEDPVHRLFVTAFSVCCECLAWTSLF--SNISPEN 390
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,120,496
Number of Sequences: 5004
Number of extensions: 60823
Number of successful extensions: 174
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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