BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0201 (809 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 61 2e-10 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 51 2e-07 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 44 3e-05 SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 42 8e-05 SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 40 4e-04 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.005 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.007 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.78 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 1.8 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 7.3 SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.6 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 60.9 bits (141), Expect = 2e-10 Identities = 32/87 (36%), Positives = 50/87 (57%), Gaps = 1/87 (1%) Frame = +2 Query: 284 EESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTG 460 E+ I L +VD T+E DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ Sbjct: 69 EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL- 127 Query: 461 PPAVEVTSAEQAKELIMPILLLYLVSF 541 P V+ S + + + L +V+F Sbjct: 128 LPTVKPISKDTLENFVEKADDLAVVAF 154 Score = 55.2 bits (127), Expect = 1e-08 Identities = 22/41 (53%), Positives = 33/41 (80%), Gaps = 1/41 (2%) Frame = +3 Query: 132 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAP 251 ++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAP 393 Score = 50.8 bits (116), Expect = 2e-07 Identities = 18/34 (52%), Positives = 26/34 (76%) Frame = +3 Query: 153 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPE 254 ++K +IT + ++V+FYAPWCGHCK+LAPE Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPE 60 Score = 41.9 bits (94), Expect = 1e-04 Identities = 25/66 (37%), Positives = 42/66 (63%), Gaps = 6/66 (9%) Frame = +2 Query: 272 KLAEE---ESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDI 433 KLAEE +S + +AK+DAT E D++ S + G+PT+ FF+ +P+ Y G R +D+ Sbjct: 397 KLAEEYSDDSNVVVAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDL 453 Query: 434 ISWLKK 451 +++ K Sbjct: 454 SAFIDK 459 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 50.8 bits (116), Expect = 2e-07 Identities = 23/64 (35%), Positives = 39/64 (60%), Gaps = 2/64 (3%) Frame = +2 Query: 275 LAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQADDIISWLK 448 L E+ + + + K+DA D+A+ Y + G+PTL +F +GS P+ YS R D + ++ Sbjct: 67 LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVS 126 Query: 449 KKTG 460 +KTG Sbjct: 127 EKTG 130 Score = 46.4 bits (105), Expect = 5e-06 Identities = 20/38 (52%), Positives = 26/38 (68%), Gaps = 1/38 (2%) Frame = +3 Query: 141 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAP 251 NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAP 178 Score = 44.4 bits (100), Expect = 2e-05 Identities = 23/63 (36%), Positives = 33/63 (52%) Frame = +3 Query: 63 IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 242 + + +L F AL L V +++ L T+ + + L+EFYA WCGHCKS Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56 Query: 243 LAP 251 LAP Sbjct: 57 LAP 59 Score = 39.9 bits (89), Expect = 4e-04 Identities = 19/66 (28%), Positives = 35/66 (53%), Gaps = 3/66 (4%) Frame = +2 Query: 272 KLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISW 442 K+ + E +++ K++A D+ + V +PT+KFF P Y G R + +I + Sbjct: 185 KVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEY 244 Query: 443 LKKKTG 460 + KK+G Sbjct: 245 INKKSG 250 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 43.6 bits (98), Expect = 3e-05 Identities = 20/58 (34%), Positives = 26/58 (44%) Frame = +3 Query: 78 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 251 L +L+ G N + L+ NF + LV FYAPWCG+CK L P Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVP 68 Score = 30.3 bits (65), Expect = 0.34 Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 6/72 (8%) Frame = +2 Query: 293 PIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGRQADDIISWLKKK 454 P+ DA Q + + Y V+G+PT+K GS + DY+G R + ++ Sbjct: 82 PVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDS 141 Query: 455 TGPPAVEVTSAE 490 P V++ ++E Sbjct: 142 I-PSKVKILTSE 152 >SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 42.3 bits (95), Expect = 8e-05 Identities = 18/88 (20%), Positives = 47/88 (53%), Gaps = 1/88 (1%) Frame = +2 Query: 296 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVE 475 +K+A+V+ +E+++ + ++ +PT + F+ I Y+G + ++S+ + A++ Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289 Query: 476 VTSAE-QAKELIMPILLLYLVSFRTRAQ 556 + + ++ E P+ L L F T ++ Sbjct: 290 IEEGDIESIENSHPVFFLVLYDFATTSE 317 Score = 28.3 bits (60), Expect = 1.4 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%) Frame = +2 Query: 335 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 481 LA YG + P++ RNG PI Y R+ D I W+ + P E+T Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 39.9 bits (89), Expect = 4e-04 Identities = 12/35 (34%), Positives = 25/35 (71%) Frame = +3 Query: 150 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPE 254 V + F++++ + ++V+F+A WCG CK++AP+ Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPK 39 Score = 27.1 bits (57), Expect = 3.2 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = +2 Query: 308 KVDATQEQDLAESYGVRGYPTLKFFRNGSPID 403 KVD Q ++A GV P+ ++NG I+ Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 36.3 bits (80), Expect = 0.005 Identities = 15/34 (44%), Positives = 21/34 (61%) Frame = +3 Query: 150 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 251 V S ++ T I+ + +V+FYA WCG CK L P Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP 55 Score = 36.3 bits (80), Expect = 0.005 Identities = 19/60 (31%), Positives = 28/60 (46%) Frame = +2 Query: 272 KLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 451 KL+E+ V+A + D+A+ GV PT+ FR G +D G + S L K Sbjct: 59 KLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 35.9 bits (79), Expect = 0.007 Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 4/69 (5%) Frame = +2 Query: 305 AKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAV 472 AKV+ +++ +A GV+ PT FF NG ID G QA + + K TG A+ Sbjct: 56 AKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGAL 115 Query: 473 EVTSAEQAK 499 +S+ K Sbjct: 116 ASSSSAPVK 124 Score = 33.9 bits (74), Expect = 0.027 Identities = 12/34 (35%), Positives = 22/34 (64%) Frame = +3 Query: 150 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 251 + S ++ + I + Y+ V+ YA WCG CK+++P Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISP 39 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 29.1 bits (62), Expect = 0.78 Identities = 10/52 (19%), Positives = 28/52 (53%) Frame = +2 Query: 296 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 451 + +A ++ + + Y ++ +PT FF+ + ++Y G D++S+ ++ Sbjct: 331 LNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382 Score = 25.8 bits (54), Expect = 7.3 Identities = 8/16 (50%), Positives = 11/16 (68%) Frame = +3 Query: 204 VEFYAPWCGHCKSLAP 251 +++Y P CG CK L P Sbjct: 47 IKYYLPSCGACKRLGP 62 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 27.9 bits (59), Expect = 1.8 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +3 Query: 192 EYILVEFYAPWCGHCKSL 245 + IL+ FYAPW CK + Sbjct: 21 QIILLNFYAPWAAPCKQM 38 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 25.8 bits (54), Expect = 7.3 Identities = 16/38 (42%), Positives = 19/38 (50%) Frame = -2 Query: 793 SFSENSTISRLLWTTPKHXADPPGISSSSYLTRFSSKV 680 S S NSTIS +T + G SSSS T +S V Sbjct: 203 SASSNSTISGFSTSTTSASSSAAGNSSSSSYTSYSGAV 240 >SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 977 Score = 25.4 bits (53), Expect = 9.6 Identities = 17/59 (28%), Positives = 27/59 (45%) Frame = +2 Query: 221 MVRPLQISGTGNAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSP 397 + R SG + L+++ +K D+T Q+L ES G YP++ FF P Sbjct: 635 LARASLTSGLKSNNTLRALSQDLKNCAKSKDDSTT-QNLTESLGSVCYPSMPFFNQYVP 692 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,021,012 Number of Sequences: 5004 Number of extensions: 57348 Number of successful extensions: 198 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 197 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 394431430 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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