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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0201
         (809 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    61   2e-10
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    51   2e-07
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    44   3e-05
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ...    42   8e-05
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    40   4e-04
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    36   0.005
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    36   0.007
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    29   0.78 
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    28   1.8  
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb...    26   7.3  
SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    25   9.6  

>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 60.9 bits (141), Expect = 2e-10
 Identities = 32/87 (36%), Positives = 50/87 (57%), Gaps = 1/87 (1%)
 Frame = +2

Query: 284 EESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTG 460
           E+  I L +VD T+E DL   Y +RGYPTL  F+NG  I  YSG R+ D ++ +++K+  
Sbjct: 69  EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL- 127

Query: 461 PPAVEVTSAEQAKELIMPILLLYLVSF 541
            P V+  S +  +  +     L +V+F
Sbjct: 128 LPTVKPISKDTLENFVEKADDLAVVAF 154



 Score = 55.2 bits (127), Expect = 1e-08
 Identities = 22/41 (53%), Positives = 33/41 (80%), Gaps = 1/41 (2%)
 Frame = +3

Query: 132 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAP 251
           ++E+++VL   NF+ ++   T+ +LVEFYAPWCGHCK+LAP
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAP 393



 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 18/34 (52%), Positives = 26/34 (76%)
 Frame = +3

Query: 153 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPE 254
           ++K     +IT  + ++V+FYAPWCGHCK+LAPE
Sbjct: 27  VNKEGLNELITADKVLMVKFYAPWCGHCKALAPE 60



 Score = 41.9 bits (94), Expect = 1e-04
 Identities = 25/66 (37%), Positives = 42/66 (63%), Gaps = 6/66 (9%)
 Frame = +2

Query: 272 KLAEE---ESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDI 433
           KLAEE   +S + +AK+DAT E D++ S  + G+PT+ FF+     +P+ Y G R  +D+
Sbjct: 397 KLAEEYSDDSNVVVAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDL 453

Query: 434 ISWLKK 451
            +++ K
Sbjct: 454 SAFIDK 459


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 23/64 (35%), Positives = 39/64 (60%), Gaps = 2/64 (3%)
 Frame = +2

Query: 275 LAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQADDIISWLK 448
           L E+ + + + K+DA    D+A+ Y + G+PTL +F  +GS P+ YS  R  D +  ++ 
Sbjct: 67  LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVS 126

Query: 449 KKTG 460
           +KTG
Sbjct: 127 EKTG 130



 Score = 46.4 bits (105), Expect = 5e-06
 Identities = 20/38 (52%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
 Frame = +3

Query: 141 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAP 251
           NV+ L   NF+ V+   +  +LVEFYA WCG+CK LAP
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAP 178



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 23/63 (36%), Positives = 33/63 (52%)
 Frame = +3

Query: 63  IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 242
           + + +L F   AL  L     V   +++  L      T+  + +  L+EFYA WCGHCKS
Sbjct: 1   MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56

Query: 243 LAP 251
           LAP
Sbjct: 57  LAP 59



 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 19/66 (28%), Positives = 35/66 (53%), Gaps = 3/66 (4%)
 Frame = +2

Query: 272 KLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISW 442
           K+ + E  +++ K++A    D+   + V  +PT+KFF       P  Y G R  + +I +
Sbjct: 185 KVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEY 244

Query: 443 LKKKTG 460
           + KK+G
Sbjct: 245 INKKSG 250


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 20/58 (34%), Positives = 26/58 (44%)
 Frame = +3

Query: 78  LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 251
           L     +L+    G       N + L+  NF   +      LV FYAPWCG+CK L P
Sbjct: 11  LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVP 68



 Score = 30.3 bits (65), Expect = 0.34
 Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 6/72 (8%)
 Frame = +2

Query: 293 PIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGRQADDIISWLKKK 454
           P+     DA Q + +   Y V+G+PT+K       GS +   DY+G R    +  ++   
Sbjct: 82  PVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDS 141

Query: 455 TGPPAVEVTSAE 490
             P  V++ ++E
Sbjct: 142 I-PSKVKILTSE 152


>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 632

 Score = 42.3 bits (95), Expect = 8e-05
 Identities = 18/88 (20%), Positives = 47/88 (53%), Gaps = 1/88 (1%)
 Frame = +2

Query: 296 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVE 475
           +K+A+V+  +E+++   + ++ +PT + F+    I Y+G  +   ++S+  +     A++
Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289

Query: 476 VTSAE-QAKELIMPILLLYLVSFRTRAQ 556
           +   + ++ E   P+  L L  F T ++
Sbjct: 290 IEEGDIESIENSHPVFFLVLYDFATTSE 317



 Score = 28.3 bits (60), Expect = 1.4
 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
 Frame = +2

Query: 335 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 481
           LA  YG +  P++   RNG PI Y     R+  D   I  W+   + P   E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 12/35 (34%), Positives = 25/35 (71%)
 Frame = +3

Query: 150 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPE 254
           V   + F++++   + ++V+F+A WCG CK++AP+
Sbjct: 5   VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPK 39



 Score = 27.1 bits (57), Expect = 3.2
 Identities = 11/32 (34%), Positives = 17/32 (53%)
 Frame = +2

Query: 308 KVDATQEQDLAESYGVRGYPTLKFFRNGSPID 403
           KVD  Q  ++A   GV   P+   ++NG  I+
Sbjct: 54  KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 36.3 bits (80), Expect = 0.005
 Identities = 15/34 (44%), Positives = 21/34 (61%)
 Frame = +3

Query: 150 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 251
           V S  ++ T I+  +  +V+FYA WCG CK L P
Sbjct: 22  VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP 55



 Score = 36.3 bits (80), Expect = 0.005
 Identities = 19/60 (31%), Positives = 28/60 (46%)
 Frame = +2

Query: 272 KLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 451
           KL+E+        V+A +  D+A+  GV   PT+  FR G  +D   G     + S L K
Sbjct: 59  KLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 35.9 bits (79), Expect = 0.007
 Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 4/69 (5%)
 Frame = +2

Query: 305 AKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAV 472
           AKV+  +++ +A   GV+  PT  FF NG  ID   G   QA  + +     K TG  A+
Sbjct: 56  AKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGAL 115

Query: 473 EVTSAEQAK 499
             +S+   K
Sbjct: 116 ASSSSAPVK 124



 Score = 33.9 bits (74), Expect = 0.027
 Identities = 12/34 (35%), Positives = 22/34 (64%)
 Frame = +3

Query: 150 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 251
           + S  ++ + I  + Y+ V+ YA WCG CK+++P
Sbjct: 6   IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISP 39


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 29.1 bits (62), Expect = 0.78
 Identities = 10/52 (19%), Positives = 28/52 (53%)
 Frame = +2

Query: 296 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 451
           + +A ++    +   + Y ++ +PT  FF+  + ++Y G     D++S+ ++
Sbjct: 331 LNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382



 Score = 25.8 bits (54), Expect = 7.3
 Identities = 8/16 (50%), Positives = 11/16 (68%)
 Frame = +3

Query: 204 VEFYAPWCGHCKSLAP 251
           +++Y P CG CK L P
Sbjct: 47  IKYYLPSCGACKRLGP 62


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 9/18 (50%), Positives = 12/18 (66%)
 Frame = +3

Query: 192 EYILVEFYAPWCGHCKSL 245
           + IL+ FYAPW   CK +
Sbjct: 21  QIILLNFYAPWAAPCKQM 38


>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 262

 Score = 25.8 bits (54), Expect = 7.3
 Identities = 16/38 (42%), Positives = 19/38 (50%)
 Frame = -2

Query: 793 SFSENSTISRLLWTTPKHXADPPGISSSSYLTRFSSKV 680
           S S NSTIS    +T    +   G SSSS  T +S  V
Sbjct: 203 SASSNSTISGFSTSTTSASSSAAGNSSSSSYTSYSGAV 240


>SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 977

 Score = 25.4 bits (53), Expect = 9.6
 Identities = 17/59 (28%), Positives = 27/59 (45%)
 Frame = +2

Query: 221 MVRPLQISGTGNAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSP 397
           + R    SG  +      L+++      +K D+T  Q+L ES G   YP++ FF    P
Sbjct: 635 LARASLTSGLKSNNTLRALSQDLKNCAKSKDDSTT-QNLTESLGSVCYPSMPFFNQYVP 692


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,021,012
Number of Sequences: 5004
Number of extensions: 57348
Number of successful extensions: 198
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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