Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0174
(708 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 151 3e-37
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 151 3e-37
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 151 3e-37
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 151 3e-37
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 98 6e-21
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 92 4e-19
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 69 2e-12
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 66 2e-11
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 36 0.026
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 36 0.026
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 36 0.026
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 36 0.026
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 36 0.026
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 35 0.061
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 35 0.061
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 35 0.061
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 33 0.19
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.19
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.25
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.25
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 31 0.99
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.7
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.7
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.7
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.3
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.3
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.3
At1g78850.1 68414.m09191 curculin-like (mannose-binding) lectin ... 28 5.3
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 28 7.0
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 7.0
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 28 7.0
At1g78860.1 68414.m09192 curculin-like (mannose-binding) lectin ... 28 7.0
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 7.0
At5g62170.1 68418.m07804 expressed protein various predicted pro... 27 9.2
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 9.2
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 9.2
At3g05470.1 68416.m00599 formin homology 2 domain-containing pro... 27 9.2
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 151 bits (367), Expect = 3e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 438 GQTREHALLAFTLGVKQLIVGVNK 509
GQTREHALLAFTLGVKQ+I NK
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNK 154
Score = 130 bits (313), Expect = 1e-30
Identities = 61/68 (89%), Positives = 62/68 (91%)
Frame = +1
Query: 49 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 228
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 229 DKLKAERE 252
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 74.9 bits (176), Expect = 5e-14
Identities = 31/49 (63%), Positives = 41/49 (83%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML 652
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMI 202
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 151 bits (367), Expect = 3e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 438 GQTREHALLAFTLGVKQLIVGVNK 509
GQTREHALLAFTLGVKQ+I NK
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNK 154
Score = 130 bits (313), Expect = 1e-30
Identities = 61/68 (89%), Positives = 62/68 (91%)
Frame = +1
Query: 49 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 228
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 229 DKLKAERE 252
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 74.9 bits (176), Expect = 5e-14
Identities = 31/49 (63%), Positives = 41/49 (83%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML 652
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMI 202
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 151 bits (367), Expect = 3e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 438 GQTREHALLAFTLGVKQLIVGVNK 509
GQTREHALLAFTLGVKQ+I NK
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNK 154
Score = 130 bits (313), Expect = 1e-30
Identities = 61/68 (89%), Positives = 62/68 (91%)
Frame = +1
Query: 49 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 228
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 229 DKLKAERE 252
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 74.9 bits (176), Expect = 5e-14
Identities = 31/49 (63%), Positives = 41/49 (83%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML 652
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMI 202
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 151 bits (367), Expect = 3e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 438 GQTREHALLAFTLGVKQLIVGVNK 509
GQTREHALLAFTLGVKQ+I NK
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNK 154
Score = 130 bits (313), Expect = 1e-30
Identities = 61/68 (89%), Positives = 62/68 (91%)
Frame = +1
Query: 49 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 228
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 229 DKLKAERE 252
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 74.9 bits (176), Expect = 5e-14
Identities = 31/49 (63%), Positives = 41/49 (83%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML 652
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMI 202
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 97.9 bits (233), Expect = 6e-21
Identities = 45/83 (54%), Positives = 58/83 (69%)
Frame = +3
Query: 261 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 440
T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G + G
Sbjct: 166 TVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERGG 225
Query: 441 QTREHALLAFTLGVKQLIVGVNK 509
QTREH LA TLGV +LIV VNK
Sbjct: 226 QTREHVQLAKTLGVSKLIVVVNK 248
Score = 68.1 bits (159), Expect = 5e-12
Identities = 28/64 (43%), Positives = 46/64 (71%)
Frame = +1
Query: 58 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 237
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 238 KAER 249
+ ER
Sbjct: 158 EEER 161
Score = 39.5 bits (88), Expect = 0.002
Identities = 19/49 (38%), Positives = 31/49 (63%), Gaps = 1/49 (2%)
Frame = +2
Query: 506 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNM 649
+MD +S+ R++EI++++ ++K GYN V F+PISG G NM
Sbjct: 248 KMDDPTVNWSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNM 296
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 91.9 bits (218), Expect = 4e-19
Identities = 40/85 (47%), Positives = 60/85 (70%), Gaps = 1/85 (1%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK- 434
IT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG
Sbjct: 303 ITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDNL 362
Query: 435 NGQTREHALLAFTLGVKQLIVGVNK 509
GQTREHA + GV+Q+IV +NK
Sbjct: 363 KGQTREHARVLRGFGVEQVIVAINK 387
Score = 77.4 bits (182), Expect = 9e-15
Identities = 32/63 (50%), Positives = 46/63 (73%)
Frame = +1
Query: 64 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 243
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 244 ERE 252
ERE
Sbjct: 298 ERE 300
Score = 34.7 bits (76), Expect = 0.061
Identities = 12/43 (27%), Positives = 27/43 (62%)
Frame = +2
Query: 530 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLGA 658
YS+ RF+ IK+ V S+++ + +++ ++P+S N++ A
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAA 435
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 69.3 bits (162), Expect = 2e-12
Identities = 35/84 (41%), Positives = 51/84 (60%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G
Sbjct: 128 ITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP------ 181
Query: 438 GQTREHALLAFTLGVKQLIVGVNK 509
QT+EH LLA +GV ++V +NK
Sbjct: 182 -QTKEHILLAKQVGVPDMVVFLNK 204
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 46 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 180
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 66.1 bits (154), Expect = 2e-11
Identities = 35/84 (41%), Positives = 50/84 (59%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 116 ITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP------ 169
Query: 438 GQTREHALLAFTLGVKQLIVGVNK 509
QT+EH LLA +GV L+ +NK
Sbjct: 170 -QTKEHILLARQVGVPSLVCFLNK 192
Score = 32.7 bits (71), Expect = 0.25
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +1
Query: 55 KEKTHINIVVIGHVDSGKSTTT 120
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 35.9 bits (79), Expect = 0.026
Identities = 24/93 (25%), Positives = 45/93 (48%)
Frame = +3
Query: 291 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 470
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 471 TLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKY 569
+ +K +I+ NK I L + + + ++ +K+
Sbjct: 178 MMRLKHIIILQNK-IDLINEKAATEQHEAIQKF 209
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 35.9 bits (79), Expect = 0.026
Identities = 24/93 (25%), Positives = 45/93 (48%)
Frame = +3
Query: 291 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 470
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 471 TLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKY 569
+ +K +I+ NK I L + + + ++ +K+
Sbjct: 178 MMRLKHIIILQNK-IDLINEKAATEQHEAIQKF 209
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 35.9 bits (79), Expect = 0.026
Identities = 26/89 (29%), Positives = 37/89 (41%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITI A Y V IID PGH DF + D A+L++ + G I+ +
Sbjct: 119 ITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVD 178
Query: 438 GQTREHALLAFTLGVKQLIVGVNKWIPLN 524
Q R + + K +G + W LN
Sbjct: 179 RQMRRYEVPRVAFINKLDRMGADPWKVLN 207
Score = 27.9 bits (59), Expect = 7.0
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 73 NIVVIGHVDSGKSTTTGHLIYKCGGI 150
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 35.9 bits (79), Expect = 0.026
Identities = 26/89 (29%), Positives = 37/89 (41%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 437
ITI A Y V IID PGH DF + D A+L++ + G I+ +
Sbjct: 119 ITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVD 178
Query: 438 GQTREHALLAFTLGVKQLIVGVNKWIPLN 524
Q R + + K +G + W LN
Sbjct: 179 RQMRRYEVPRVAFINKLDRMGADPWKVLN 207
Score = 27.9 bits (59), Expect = 7.0
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 73 NIVVIGHVDSGKSTTTGHLIYKCGGI 150
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 35.9 bits (79), Expect = 0.026
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 4/99 (4%)
Frame = +3
Query: 285 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 452
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 453 HALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKY 569
H + +K +I+ NK I L V+ + ++ +K+
Sbjct: 166 HLAAVEIMQLKHIIILQNK-IDLIQENVAINQHEAIQKF 203
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 34.7 bits (76), Expect = 0.061
Identities = 21/69 (30%), Positives = 33/69 (47%)
Frame = +3
Query: 303 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 482
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 483 KQLIVGVNK 509
K +I+ NK
Sbjct: 174 KDIIIIQNK 182
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 34.7 bits (76), Expect = 0.061
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 410
+ + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 195 VPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 34.7 bits (76), Expect = 0.061
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 410
+ + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 195 VPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 33.1 bits (72), Expect = 0.19
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +3
Query: 288 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 410
E S Y + +ID PGH DF + S A+L+V A G
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG 171
Score = 27.9 bits (59), Expect = 7.0
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +1
Query: 37 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 156
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 33.1 bits (72), Expect = 0.19
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = +3
Query: 258 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 410
IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 60 ITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
Score = 32.7 bits (71), Expect = 0.25
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 73 NIVVIGHVDSGKSTTTGHLIYKCGG 147
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.25
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 306 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 410
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.25
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +1
Query: 61 KTHINIVVIGHVDSGKSTTTGHLIYKCG 144
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 30.7 bits (66), Expect = 0.99
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +3
Query: 285 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 410
+E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 147 YEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
Score = 28.3 bits (60), Expect = 5.3
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +1
Query: 73 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 171
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 312 IIDAPGHRDFIKNMITGTSQADCAVLIV 395
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 312 IIDAPGHRDFIKNMITGTSQADCAVLIV 395
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 312 IIDAPGHRDFIKNMITGTSQADCAVLIV 395
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.3
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 294 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 395
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 3.0
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +1
Query: 73 NIVVIGHVDSGKSTTTGHLIYKCG 144
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.3
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +3
Query: 306 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 395
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.3
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +2
Query: 533 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 622
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At1g78850.1 68414.m09191 curculin-like (mannose-binding) lectin
family protein low similarity to ser/thr protein kinase
from Zea mays [GI:2598067]; contains Pfam lectin
(probable mannose binding) domain PF01453
Length = 441
Score = 28.3 bits (60), Expect = 5.3
Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = +1
Query: 121 GHLIYKCGGIDKRT--IEKFEKEAQEMGKGSFKYAWVLDKLKAERELVSQSILLSGSSKL 294
G L+++ +K I+ E + S K+ W + LV QS+ L+G +KL
Sbjct: 120 GRLVWQTNTANKGAVGIKILENGNMVIYDSSGKFVWQSFDSPTDTLLVGQSLKLNGRTKL 179
Query: 295 ASTMLPSL 318
S + PS+
Sbjct: 180 VSRLSPSV 187
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 27.9 bits (59), Expect = 7.0
Identities = 18/66 (27%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = +2
Query: 476 RCQTAHRRSKQMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISG-WHGDNML 652
R T+H + K ++STE E KEV ++K +GY+ + V ++ + GD+ L
Sbjct: 313 RESTSHWKIKSINSTEQR-EEGLCPLTPKEVGIFLKGLGYSQSTVIYIAAGEIYGGDDRL 371
Query: 653 GAFQPK 670
+ +
Sbjct: 372 SELKSR 377
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 7.0
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 539 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 649
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 27.9 bits (59), Expect = 7.0
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = -2
Query: 398 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCDTSSRSAFSLSN 225
++D+HS +L+ D + D + ++ N+VL F P+ D T +R A L N
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR-AIELPN 108
>At1g78860.1 68414.m09192 curculin-like (mannose-binding) lectin
family protein low similarity to Ser/Thr protein kinase
[Zea mays] GI:2598067; contains Pfam profile PF01453:
Lectin (probable mannose binding)
Length = 443
Score = 27.9 bits (59), Expect = 7.0
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 211 KYAWVLDKLKAERELVSQSILLSGSSKLASTMLPSL 318
K+ W + LV QS+ L+G +KL S + PS+
Sbjct: 152 KFVWQSFDSPTDTLLVGQSLKLNGQNKLVSRLSPSV 187
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 7.0
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +3
Query: 315 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 410
+D PGH F G D A+++VAA G
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG 588
>At5g62170.1 68418.m07804 expressed protein various predicted
proteins, Arabidopsis thaliana
Length = 703
Score = 27.5 bits (58), Expect = 9.2
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = -3
Query: 703 FPPAHPLEPKAFWLKGSQHVVSVPSRNGHES 611
F P+HPL+P F+ V S RN E+
Sbjct: 513 FSPSHPLDPSIFYFLEHFAVTSTRPRNSPEN 543
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 9.2
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 515 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 628
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 9.2
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 515 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 628
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At3g05470.1 68416.m00599 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 884
Score = 27.5 bits (58), Expect = 9.2
Identities = 24/85 (28%), Positives = 35/85 (41%)
Frame = -2
Query: 398 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCDTSSRSAFSLSNTQ 219
S D+ S S+ G + +S + S + V +S E +D SRS F +S +
Sbjct: 308 SDDDESFHSVGGGSQYSNPRLSNASSASGSVNVGSSQRFSEHKLDIPECSRSDFGISVSA 367
Query: 218 AYLKDPLPISWASFSNFSMVRLSIP 144
P P FSN + LS P
Sbjct: 368 PPPPPPPPPPLPQFSNKRIHTLSSP 392
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,426,010
Number of Sequences: 28952
Number of extensions: 353197
Number of successful extensions: 1214
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 1151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1210
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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