Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0165
(713 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 62 6e-11
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 51 2e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 49 8e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 41 2e-04
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 5e-04
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 36 0.004
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.004
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 31 0.22
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce... 28 1.5
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 1.5
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 2.0
SPBC4B4.11 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.5
SPAC2G11.15c |tgs1|SPAC521.01c|RNA methyltransferase Tgs1 |Schiz... 26 4.7
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.1
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 25 8.1
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 62.5 bits (145), Expect = 6e-11
Identities = 32/84 (38%), Positives = 50/84 (59%), Gaps = 2/84 (2%)
Frame = +3
Query: 261 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTGPPAV 437
I L +VD T+E DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ P V
Sbjct: 73 ISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL-LPTV 131
Query: 438 EVTSAEQAKELID-ANTVIVFGFF 506
+ S + + ++ A+ + V FF
Sbjct: 132 KPISKDTLENFVEKADDLAVVAFF 155
Score = 62.1 bits (144), Expect = 8e-11
Identities = 25/53 (47%), Positives = 40/53 (75%), Gaps = 1/53 (1%)
Frame = +1
Query: 97 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAPEYAKAATKLAEE 252
++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + +++
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDD 405
Score = 59.3 bits (137), Expect = 5e-10
Identities = 22/47 (46%), Positives = 34/47 (72%)
Frame = +1
Query: 118 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEDL 258
++K +IT + ++V+FYAPWCGHCK+LAPEY AA +L ++ +
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGI 73
Score = 37.9 bits (84), Expect = 0.001
Identities = 20/57 (35%), Positives = 36/57 (63%), Gaps = 3/57 (5%)
Frame = +3
Query: 255 SPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDIISWLKK 416
S + +AK+DAT E D++ S + G+PT+ FF+ +P+ Y G R +D+ +++ K
Sbjct: 406 SNVVVAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 50.8 bits (116), Expect = 2e-07
Identities = 24/67 (35%), Positives = 31/67 (46%)
Frame = +1
Query: 43 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 222
L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 223 AKAATKL 243
K A+ L
Sbjct: 71 QKLASNL 77
Score = 30.3 bits (65), Expect = 0.29
Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 6/72 (8%)
Frame = +3
Query: 258 PIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGRQADDIISWLKKK 419
P+ DA Q + + Y V+G+PT+K GS + DY+G R + ++
Sbjct: 82 PVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDS 141
Query: 420 TGPPAVEVTSAE 455
P V++ ++E
Sbjct: 142 I-PSKVKILTSE 152
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 48.8 bits (111), Expect = 8e-07
Identities = 21/40 (52%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
Frame = +1
Query: 106 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAPEY 222
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180
Score = 48.0 bits (109), Expect = 1e-06
Identities = 24/76 (31%), Positives = 40/76 (52%), Gaps = 2/76 (2%)
Frame = +3
Query: 261 IKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQADDIISWLKKKTGPPA 434
+ + K+DA D+A+ Y + G+PTL +F +GS P+ YS R D + ++ +KTG
Sbjct: 74 VLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKK 133
Query: 435 VEVTSAEQAKELIDAN 482
++ EL N
Sbjct: 134 RKIVLPSNVVELDSLN 149
Score = 46.4 bits (105), Expect = 4e-06
Identities = 24/65 (36%), Positives = 34/65 (52%)
Frame = +1
Query: 28 IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 207
+ + +L F AL L V +++ L T+ + + L+EFYA WCGHCKS
Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56
Query: 208 LAPEY 222
LAP Y
Sbjct: 57 LAPVY 61
Score = 37.1 bits (82), Expect = 0.002
Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
Frame = +3
Query: 261 IKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISWLKKKTG 425
+++ K++A D+ + V +PT+KFF P Y G R + +I ++ KK+G
Sbjct: 193 VEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 41.1 bits (92), Expect = 2e-04
Identities = 12/36 (33%), Positives = 26/36 (72%)
Frame = +1
Query: 115 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 222
V + F++++ + ++V+F+A WCG CK++AP++
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40
Score = 27.1 bits (57), Expect = 2.7
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +3
Query: 273 KVDATQEQDLAESYGVRGYPTLKFFRNGSPID 368
KVD Q ++A GV P+ ++NG I+
Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.5 bits (88), Expect = 5e-04
Identities = 15/44 (34%), Positives = 29/44 (65%)
Frame = +1
Query: 115 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLA 246
+ S ++ + I + Y+ V+ YA WCG CK+++P +++ A+K A
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYA 49
Score = 35.9 bits (79), Expect = 0.006
Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 4/69 (5%)
Frame = +3
Query: 270 AKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAV 437
AKV+ +++ +A GV+ PT FF NG ID G QA + + K TG A+
Sbjct: 56 AKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGAL 115
Query: 438 EVTSAEQAK 464
+S+ K
Sbjct: 116 ASSSSAPVK 124
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 36.3 bits (80), Expect = 0.004
Identities = 12/65 (18%), Positives = 36/65 (55%)
Frame = +3
Query: 261 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVE 440
+K+A+V+ +E+++ + ++ +PT + F+ I Y+G + ++S+ + A++
Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289
Query: 441 VTSAE 455
+ +
Sbjct: 290 IEEGD 294
Score = 28.3 bits (60), Expect = 1.2
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
Frame = +3
Query: 300 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 446
LA YG + P++ RNG PI Y R+ D I W+ + P E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 36.3 bits (80), Expect = 0.004
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = +1
Query: 115 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 216
V S ++ T I+ + +V+FYA WCG CK L P
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP 55
Score = 31.1 bits (67), Expect = 0.16
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +3
Query: 276 VDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 416
V+A + D+A+ GV PT+ FR G +D G + S L K
Sbjct: 72 VNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 30.7 bits (66), Expect = 0.22
Identities = 11/55 (20%), Positives = 29/55 (52%)
Frame = +3
Query: 252 RSPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 416
R + +A ++ + + Y ++ +PT FF+ + ++Y G D++S+ ++
Sbjct: 328 RGKLNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382
Score = 28.3 bits (60), Expect = 1.2
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = +1
Query: 169 VEFYAPWCGHCKSLAPEYAKAATKLAEE 252
+++Y P CG CK L P + K E+
Sbjct: 47 IKYYLPSCGACKRLGPMWDNMVEKAKEQ 74
>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1210
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -3
Query: 708 SKNPQVNFFXPLKFDTFLFEVLEKHNI 628
S N ++N F PLKFD L + E N+
Sbjct: 319 SYNDRLNAFGPLKFDALLNFITEMRNV 345
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 27.9 bits (59), Expect = 1.5
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 157 EYILVEFYAPWCGHCKSL 210
+ IL+ FYAPW CK +
Sbjct: 21 QIILLNFYAPWAAPCKQM 38
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 27.5 bits (58), Expect = 2.0
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +2
Query: 104 KMCSF*VKLTLKL*LQPRSTF*LNSMLHGAATANLWHRNTP 226
K +F L LQP T N +L+ + NLW R+ P
Sbjct: 620 KSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLP 660
>SPBC4B4.11 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 113
Score = 26.6 bits (56), Expect = 3.5
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -1
Query: 569 LGHQQLEQLKGKFWLWLSSGRKETKYNNSIGID 471
+G E++ GKF W RK TK GID
Sbjct: 43 IGRDTAEKVNGKFESWDGLFRKSTKTMKKEGID 75
>SPAC2G11.15c |tgs1|SPAC521.01c|RNA methyltransferase Tgs1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 239
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/46 (26%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = -3
Query: 645 LEKHNIFIFSLPTPLITFSSLTIANTWSSTT*AVE-RKVLALAELW 511
+ KHN+ I+ +P+ +TF + +T+ S A + R ++ ++ W
Sbjct: 108 MAKHNLEIYGIPSSRVTFIQGDVLDTFKSLQFAKDYRSLVFMSPPW 153
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1379
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = +3
Query: 429 PAVEVTSAEQAKELIDANTVIVFGFFSTRAQPEPK 533
PAVE +K+ +A ++ F FF+ +P K
Sbjct: 219 PAVEKLKTSASKQAAEAQSLREFDFFTPTPEPSDK 253
>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1367
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +2
Query: 200 ANLWHRNTPRQQQSWLKKISYQTSES*RNSRTGSRRELRCTRIPD 334
AN + + QSW +++ + +E+ S S R + T IP+
Sbjct: 74 ANSFEGSCNNSDQSWTSRVTSKKNEAGTESGDASVRRIYVTSIPE 118
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,687,911
Number of Sequences: 5004
Number of extensions: 52505
Number of successful extensions: 182
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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