Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0163
(710 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 165 3e-41
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 162 3e-40
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 140 1e-33
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 132 2e-31
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 85 4e-17
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 81 9e-16
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 79 5e-15
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 42 4e-04
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 7e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.92
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.8
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 4.9
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 165 bits (401), Expect = 3e-41
Identities = 103/210 (49%), Positives = 120/210 (57%), Gaps = 4/210 (1%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 239 MNPNNKYSMPNVSSDVSSKMLLCKPT*STGLSR--LSVXEANLRSR*HIRVK--TKPFSP 406
MNP N ++ + + K P + ++ V R + + K K F P
Sbjct: 61 MNPEN--TVFDAKRLIGRK--FDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFP 116
Query: 407 RKSVPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNVLRIINEP 586
+ + P YF + + K GLNVLRIINEP
Sbjct: 117 EEISSMVLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLRIINEP 176
Query: 587 TAAAIAYGLGQKGYWENEMYFIFDFGGGTF 676
TAAAIAYGL +K E + I+D GGGTF
Sbjct: 177 TAAAIAYGLDKKVGVERNV-LIYDLGGGTF 205
Score = 165 bits (401), Expect = 3e-41
Identities = 75/105 (71%), Positives = 87/105 (82%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGRKF+D V DM HWPF V+ G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558
VLTKMKETAEAYLG V +AV+TVPA HDSQRQATKDAGTI+GL
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGL 167
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 162 bits (393), Expect = 3e-40
Identities = 76/105 (72%), Positives = 90/105 (85%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ IFDAKRLIGR+++DATVQ+DMK WPF+++S KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558
VL KMKETAEAYLG+ V +AVITVPA +DSQRQATKDAGTI+GL
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGL 167
Score = 159 bits (385), Expect = 3e-39
Identities = 94/208 (45%), Positives = 116/208 (55%), Gaps = 2/208 (0%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 239 MNPNNKYSMPNVSSDVSSKMLLCKPT*STGLSRLSVXEANLRSR*HIRVK--TKPFSPRK 412
+NP N ++ + + + L + N + + + K K F+ +
Sbjct: 61 LNPEN--TIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEE 118
Query: 413 SVPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNVLRIINEPTA 592
+ P YF + + K GLNVLR+INEPTA
Sbjct: 119 ISSMVLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLRVINEPTA 178
Query: 593 AAIAYGLGQKGYWENEMYFIFDFGGGTF 676
AA+AYGL + E + IFD GGGTF
Sbjct: 179 AALAYGLDKSLSGEKNV-LIFDLGGGTF 205
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 140 bits (338), Expect = 1e-33
Identities = 66/105 (62%), Positives = 83/105 (79%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558
VLTKMKETAEAYLGK V +AV+TVPA +D+QRQATKDAG I+GL
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGL 865
Score = 132 bits (318), Expect = 4e-31
Identities = 86/209 (41%), Positives = 111/209 (53%), Gaps = 9/209 (4%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 253
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
Query: 254 KYSMPNVSSDVSSKMLLCKPT*STGLSR------LSVXEANLRSR*HIRVK--TKPFSPR 409
+ +K L+ + T + V + N + ++VK K F+
Sbjct: 764 --------TVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAE 815
Query: 410 KSVPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNVLRIINEPT 589
+ + P YF + + K GL V+RIINEPT
Sbjct: 816 EISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGLTVMRIINEPT 875
Query: 590 AAAIAYGLGQKGYWENEMYFIFDFGGGTF 676
AAAIAYG+ +K +N +FD GGGTF
Sbjct: 876 AAAIAYGMDKKEGEKN--ILVFDLGGGTF 902
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 132 bits (320), Expect = 2e-31
Identities = 60/65 (92%), Positives = 62/65 (95%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 239 MNPNN 253
MNP N
Sbjct: 61 MNPTN 65
Score = 130 bits (314), Expect = 1e-30
Identities = 73/125 (58%), Positives = 82/125 (65%), Gaps = 25/125 (20%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEV--- 414
P IFDAKRLIGR+F+D V+ DMKHW FEVV+ G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 415 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQA 528
SSMVL KMKETAEAYLG V +AV+TVPA +DSQRQA
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQA 182
Query: 529 TKDAG 543
TKDAG
Sbjct: 183 TKDAG 187
Score = 78.6 bits (185), Expect = 5e-15
Identities = 38/50 (76%), Positives = 43/50 (86%)
Frame = +1
Query: 409 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558
++SSMVL KMKETAEAYLG V +AV+TVPA +DSQRQATKDAG ISGL
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGL 260
Score = 59.7 bits (138), Expect = 2e-09
Identities = 34/60 (56%), Positives = 38/60 (63%)
Frame = +2
Query: 497 PRYFMTLKDKPQKMQVPSLGLNVLRIINEPTAAAIAYGLGQKGYWENEMYFIFDFGGGTF 676
P YF + + K GLNVLRIINEPTAAAIAYGL +K E + IFD GGGTF
Sbjct: 240 PAYFNDSQRQATKDAGVISGLNVLRIINEPTAAAIAYGLDKKVGQERNV-LIFDLGGGTF 298
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 85.4 bits (202), Expect = 4e-17
Identities = 64/207 (30%), Positives = 95/207 (45%), Gaps = 4/207 (1%)
Frame = +2
Query: 65 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 239 MNP-NNKYSMPNVSSDVSSKMLLCKPT*STGLSRLSVXEANLRSR*HIRVKTKPFSPRKS 415
+NP N Y +K L + + N + +P +
Sbjct: 90 INPKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEI 149
Query: 416 VPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSL-GLNVLRIINEPTA 592
+ + P F +K + ++ SL G+ VLR+INEPTA
Sbjct: 150 GGYVIKKLRTMAERNLTTTVTKAVMSVPAEF-DIKQRNATVKAASLAGITVLRVINEPTA 208
Query: 593 AAIAYGLGQKGYWENEMYFIFDFGGGT 673
AA+AYGL +K N M + D GGGT
Sbjct: 209 AALAYGLHKKEGVANVM--VVDLGGGT 233
Score = 62.1 bits (144), Expect = 4e-10
Identities = 37/106 (34%), Positives = 53/106 (50%), Gaps = 1/106 (0%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXG-GKPKIKVAYKGEDKTFFPEEVSS 420
P+ I+DAKR IG+ F + K + F V + G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 421 MVLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558
V+ K++ AE L TV AV++VPA QR AT A +++G+
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPAEFDIKQRNATVKAASLAGI 197
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 81.0 bits (191), Expect = 9e-16
Identities = 38/66 (57%), Positives = 53/66 (80%)
Frame = +1
Query: 364 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAG 543
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA +D+QRQATKDAG
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAG 61
Query: 544 TISGLE 561
TI+GL+
Sbjct: 62 TIAGLD 67
Score = 36.7 bits (81), Expect = 0.019
Identities = 17/17 (100%), Positives = 17/17 (100%)
Frame = +2
Query: 563 VLRIINEPTAAAIAYGL 613
VLRIINEPTAAAIAYGL
Sbjct: 92 VLRIINEPTAAAIAYGL 108
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 78.6 bits (185), Expect = 5e-15
Identities = 41/98 (41%), Positives = 58/98 (59%), Gaps = 1/98 (1%)
Frame = +1
Query: 268 KRLIGRKFEDATVQADMKHWPFEVVSXGGKP-KIKVAYKGEDKTFFPEEVSSMVLTKMKE 444
KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M+ T++K
Sbjct: 127 KRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAMLFTRLKT 186
Query: 445 TAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558
TAE L V + VI+VP+ D QR+ DA +GL
Sbjct: 187 TAEIALKTKVTDCVISVPSYYTDRQRRCMLDASATAGL 224
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 232
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 30.7 bits (66), Expect = 1.2
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +2
Query: 497 PRYFMTLKDKPQKMQVPSLGLNVLRIINEPTAAAIAYGL 613
P Y+ + + + GLN LR++N+ TA ++AYG+
Sbjct: 204 PSYYTDRQRRCMLDASATAGLNCLRLMNDTTAVSLAYGI 242
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 42.3 bits (95), Expect = 4e-04
Identities = 21/67 (31%), Positives = 36/67 (53%)
Frame = +1
Query: 358 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKD 537
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP + ++R+A
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLR 126
Query: 538 AGTISGL 558
A + GL
Sbjct: 127 AAELVGL 133
Score = 34.3 bits (75), Expect = 0.099
Identities = 21/62 (33%), Positives = 34/62 (54%), Gaps = 3/62 (4%)
Frame = +2
Query: 497 PRYFMTLKDKPQKMQVPSLGLNVLRIINEPTAAAIAYGLGQKGYWEN--EMYFIF-DFGG 667
P +F + + +GLNVL+I+N TA A+ YGL Q+ + + E +F+F D G
Sbjct: 113 PPFFNQAERRALLRAAELVGLNVLQIMNSNTAVALNYGLFQQKSFNDTLEKHFMFYDMGA 172
Query: 668 GT 673
+
Sbjct: 173 SS 174
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 7e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +2
Query: 128 GKVEIIANDQGNRTTPSYVAFTDTERLI 211
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.92
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +2
Query: 137 EIIANDQGNRTTPSYVAFT-DTERL 208
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.8
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -1
Query: 245 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 147
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 4.9
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 5 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 91
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,887,241
Number of Sequences: 59808
Number of extensions: 536502
Number of successful extensions: 1493
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1378
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1482
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1877743452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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