BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0159 (617 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 114 8e-27 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 114 8e-27 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 102 5e-23 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 100 2e-22 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 76 4e-15 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 54 2e-08 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 43 3e-05 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 29 0.41 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.71 SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 27 2.9 SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 3.8 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 6.6 SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 25 8.8 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 8.8 SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co... 25 8.8 SPCC965.03 |vma8||V-type ATPase subunit D |Schizosaccharomyces p... 25 8.8 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 114 bits (275), Expect = 8e-27 Identities = 52/61 (85%), Positives = 57/61 (93%) Frame = +1 Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNN 252 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDT RLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 253 T 255 T Sbjct: 64 T 64 Score = 97.9 bits (233), Expect = 1e-21 Identities = 47/76 (61%), Positives = 59/76 (77%) Frame = +2 Query: 329 FXGCQXGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFN 508 F + GKP I+V +KGE KTF PEE+SSMVL KM+E+AEA+LG V +AV+TVPAYFN Sbjct: 90 FKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVPAYFN 149 Query: 509 TLKDKATKDAGTISGL 556 + +ATKDAG I+GL Sbjct: 150 DSQRQATKDAGLIAGL 165 Score = 61.7 bits (143), Expect = 8e-11 Identities = 27/56 (48%), Positives = 36/56 (64%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFXVVXLEANLRSR*HIRVKTKPFSPRK 410 P IFDAKRLIGR+F D VQ+DMKHWPF V+ + + + +TK F+P + Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEE 116 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 114 bits (275), Expect = 8e-27 Identities = 52/61 (85%), Positives = 57/61 (93%) Frame = +1 Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNN 252 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDT RLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 253 T 255 T Sbjct: 64 T 64 Score = 101 bits (242), Expect = 8e-23 Identities = 48/69 (69%), Positives = 57/69 (82%) Frame = +2 Query: 350 GKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKAT 529 GKP ++V YKGE KTF PEE+SSMVL KM+ETAEAYLG V +AV+TVPAYFN + +AT Sbjct: 97 GKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQAT 156 Query: 530 KDAGTISGL 556 KDAG I+GL Sbjct: 157 KDAGLIAGL 165 Score = 62.9 bits (146), Expect = 4e-11 Identities = 28/56 (50%), Positives = 37/56 (66%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFXVVXLEANLRSR*HIRVKTKPFSPRK 410 P IFDAKRLIGRKF+D VQ+DMKHWPF V+ + + + +TK F+P + Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEE 116 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 102 bits (244), Expect = 5e-23 Identities = 46/61 (75%), Positives = 52/61 (85%) Frame = +1 Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNN 252 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+T RL+GDAAKNQ AMNP N Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67 Query: 253 T 255 T Sbjct: 68 T 68 Score = 83.8 bits (198), Expect = 2e-17 Identities = 42/69 (60%), Positives = 51/69 (73%) Frame = +2 Query: 350 GKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKAT 529 G P I+V Y GE K F +E+S+MVLTKMKE +EA L K V+ AVITVPAYF+ + AT Sbjct: 101 GIPTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAAT 160 Query: 530 KDAGTISGL 556 KDAG I+GL Sbjct: 161 KDAGAIAGL 169 Score = 53.2 bits (122), Expect = 3e-08 Identities = 20/33 (60%), Positives = 26/33 (78%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFXVV 341 P+ +FDAKRLIGR++ED Q D+KHWPF V+ Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVI 97 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 100 bits (239), Expect = 2e-22 Identities = 44/60 (73%), Positives = 51/60 (85%) Frame = +1 Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNNT 255 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ RL+G+AAKNQ NP NT Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97 Score = 92.7 bits (220), Expect = 4e-20 Identities = 42/68 (61%), Positives = 56/68 (82%) Frame = +2 Query: 353 KPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKATK 532 +P ++V G+ K F PEE+S+M+L+KMK+TAEAYLGK V +AV+TVPAYFN + +ATK Sbjct: 131 RPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190 Query: 533 DAGTISGL 556 DAGTI+GL Sbjct: 191 DAGTIAGL 198 Score = 46.4 bits (105), Expect = 3e-06 Identities = 22/56 (39%), Positives = 34/56 (60%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFXVVXLEANLRSR*HIRVKTKPFSPRK 410 P+ IFD KRLIGRKF++ T+ D+K +PF +V + ++ K K F+P + Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEE 149 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 76.2 bits (179), Expect = 4e-15 Identities = 35/66 (53%), Positives = 46/66 (69%), Gaps = 1/66 (1%) Frame = +1 Query: 64 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTXRLIGDAAKNQVAM 240 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D RL+G +AK Q + Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 241 NPNNTY 258 NP NT+ Sbjct: 109 NPENTF 114 Score = 73.7 bits (173), Expect = 2e-14 Identities = 32/56 (57%), Positives = 43/56 (76%) Frame = +2 Query: 389 KTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKATKDAGTISGL 556 KT+ P ++ +L+KM+ETA YLGK V+NAV+TVPAYFN + +ATK AG I+GL Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGL 211 Score = 37.1 bits (82), Expect = 0.002 Identities = 15/33 (45%), Positives = 22/33 (66%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFXVV 341 P+ F KRLIGR+F++ VQ D+K P+ +V Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIV 142 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 53.6 bits (123), Expect = 2e-08 Identities = 23/65 (35%), Positives = 41/65 (63%) Frame = +1 Query: 61 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAM 240 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62 Query: 241 NPNNT 255 N NT Sbjct: 63 NFRNT 67 Score = 45.2 bits (102), Expect = 8e-06 Identities = 23/64 (35%), Positives = 38/64 (59%) Frame = +2 Query: 365 KVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKATKDAGT 544 KV Y E+ F ++ + TK+K AEA L +V + VI++PA+F ++ +A +A Sbjct: 106 KVQYLNEETAFSNIQLIAAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAAN 165 Query: 545 ISGL 556 I+GL Sbjct: 166 IAGL 169 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 43.2 bits (97), Expect = 3e-05 Identities = 18/60 (30%), Positives = 33/60 (55%) Frame = +1 Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNNT 255 +GI G S + + GK +++AN++GNR PS +++ G A+ Q+ N +N+ Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86 Score = 39.9 bits (89), Expect = 3e-04 Identities = 20/58 (34%), Positives = 31/58 (53%) Frame = +2 Query: 383 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKATKDAGTISGL 556 ++K E S L ++ E+AE +LG V V++VP YF + KA + A +GL Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGL 191 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 29.5 bits (63), Expect = 0.41 Identities = 14/55 (25%), Positives = 28/55 (50%) Frame = +2 Query: 383 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKATKDAGTI 547 +++ + EE+ +M + AE + + + V+TVP +FN L+ +A I Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185 Score = 27.9 bits (59), Expect = 1.2 Identities = 20/67 (29%), Positives = 30/67 (44%), Gaps = 1/67 (1%) Frame = +1 Query: 76 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNN 252 + ID GT ++ + + G +EI+ R S VAF R+ G A N P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82 Query: 253 TYSMPNV 273 +S+ NV Sbjct: 83 AHSIRNV 89 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.71 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +2 Query: 68 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 163 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 26.6 bits (56), Expect = 2.9 Identities = 13/40 (32%), Positives = 19/40 (47%), Gaps = 2/40 (5%) Frame = +1 Query: 424 AYENEGNCRSLSWQNCAECSYHGSRV--LQYSQRQGHKRC 537 +Y N R L W C + GSR+ + ++ Q KRC Sbjct: 266 SYRMSSNVRPLIWVECESIVHSGSRIEFMVKAKAQFKKRC 305 >SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 600 Score = 26.2 bits (55), Expect = 3.8 Identities = 14/35 (40%), Positives = 19/35 (54%) Frame = +1 Query: 157 GNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNNTYS 261 GNR+T ++ L DAAKN +A + TYS Sbjct: 349 GNRSTTNFSIINSGLYLKKDAAKNLLAKQFDATYS 383 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.4 bits (53), Expect = 6.6 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = -2 Query: 154 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 50 G W SP S + H ++++ +F +RVL C+ Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310 >SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 2244 Score = 25.0 bits (52), Expect = 8.8 Identities = 13/45 (28%), Positives = 23/45 (51%) Frame = +1 Query: 172 PSYVAFTDTXRLIGDAAKNQVAMNPNNTYSMPNVSSDVSSKMLLC 306 P +A T R++ AAK A+N Y++ ++ D K++ C Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 25.0 bits (52), Expect = 8.8 Identities = 9/26 (34%), Positives = 15/26 (57%) Frame = +3 Query: 54 QNGKSTRSRNRSGYHVLLRWCLPARE 131 +NGK T +R +S + W P++E Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE 502 >SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 25.0 bits (52), Expect = 8.8 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = +1 Query: 217 AAKNQVAMNPNNTYSMPNVSSDVSSKML 300 A K Q+ +NP + +PNV D + +L Sbjct: 323 ARKRQIQLNPFPPWDVPNVLEDQMNSLL 350 >SPCC965.03 |vma8||V-type ATPase subunit D |Schizosaccharomyces pombe|chr 3|||Manual Length = 285 Score = 25.0 bits (52), Expect = 8.8 Identities = 9/25 (36%), Positives = 17/25 (68%) Frame = +1 Query: 208 IGDAAKNQVAMNPNNTYSMPNVSSD 282 +G N+V + ++TYS+P+ S+D Sbjct: 248 VGKEVINEVENSKDDTYSLPSTSTD 272 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,567,762 Number of Sequences: 5004 Number of extensions: 51851 Number of successful extensions: 179 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 178 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 271646730 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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