BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0157
(537 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
DQ667184-1|ABG75736.1| 489|Apis mellifera GABA-gated ion channe... 23 1.5
DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 23 1.5
DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 23 1.5
AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic ac... 23 1.5
AB167961-1|BAD51404.1| 554|Apis mellifera E74 protein. 23 2.0
DQ667195-1|ABG75747.1| 469|Apis mellifera cys-loop ligand-gated... 22 3.5
DQ667182-1|ABG75734.1| 445|Apis mellifera GABA-gated chloride c... 22 3.5
DQ667181-1|ABG75733.1| 445|Apis mellifera GABA-gated chloride c... 22 3.5
DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 22 3.5
DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 22 3.5
AF144379-1|AAD34586.1| 543|Apis mellifera glutamate transporter... 22 3.5
AF094822-1|AAC63381.1| 365|Apis mellifera GABA receptor Rdl sub... 22 3.5
DQ325105-1|ABD14119.1| 180|Apis mellifera complementary sex det... 21 6.0
AF388659-4|AAK71996.1| 1308|Apis mellifera NFRKB-like protein pr... 21 6.0
DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channe... 21 8.0
DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. 21 8.0
AY352277-1|AAQ67418.1| 418|Apis mellifera complementary sex det... 21 8.0
AF084556-1|AAC71015.1| 652|Apis mellifera pipsqueak protein. 21 8.0
>DQ667184-1|ABG75736.1| 489|Apis mellifera GABA-gated ion channel
protein.
Length = 489
Score = 23.4 bits (48), Expect = 1.5
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = -2
Query: 407 YFSTSSLVIPVNCRLLASSSIFASPFLWSPTTYFASDMST 288
Y+ L +L S FA +W P T+FA+D ++
Sbjct: 91 YWKDERLAFSQEEEVLTLSGDFAEK-IWVPDTFFANDKNS 129
>DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 23.4 bits (48), Expect = 1.5
Identities = 8/24 (33%), Positives = 14/24 (58%)
Frame = +2
Query: 452 VLFWVAWLCMLAGAIAVIVRAPKL 523
+ W+ L +L G +I++AP L
Sbjct: 521 LFLWIFTLAVLVGTAGIILQAPTL 544
>DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 23.4 bits (48), Expect = 1.5
Identities = 8/24 (33%), Positives = 14/24 (58%)
Frame = +2
Query: 452 VLFWVAWLCMLAGAIAVIVRAPKL 523
+ W+ L +L G +I++AP L
Sbjct: 521 LFLWIFTLAVLVGTAGIILQAPTL 544
>AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha-3 protein.
Length = 537
Score = 23.4 bits (48), Expect = 1.5
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = +2
Query: 452 VLFWVAWLCMLAGAIAVIVRAPKL 523
+ WV L AG + +I +AP L
Sbjct: 484 LFLWVFTLACTAGTLGIIFQAPSL 507
>AB167961-1|BAD51404.1| 554|Apis mellifera E74 protein.
Length = 554
Score = 23.0 bits (47), Expect = 2.0
Identities = 13/52 (25%), Positives = 20/52 (38%)
Frame = -3
Query: 490 PGEHAQPGHPEQHETATNASSPRRDRQHTSVPPLSSSQ*IAVY*HRVQSSRH 335
P H HP Q+ +S + HT P + + H+ QS +H
Sbjct: 314 PSYHPHQHHPSQYHPHRGSSPHHQHGNHTMGPTMGPPH--HHHHHQTQSLQH 363
>DQ667195-1|ABG75747.1| 469|Apis mellifera cys-loop ligand-gated
ion channel subunit protein.
Length = 469
Score = 22.2 bits (45), Expect = 3.5
Identities = 6/21 (28%), Positives = 13/21 (61%)
Frame = -2
Query: 344 FASPFLWSPTTYFASDMSTRV 282
F +W+PT Y +++ S+ +
Sbjct: 92 FVRDLIWTPTVYVSNEPSSAI 112
>DQ667182-1|ABG75734.1| 445|Apis mellifera GABA-gated chloride
channel protein.
Length = 445
Score = 22.2 bits (45), Expect = 3.5
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = -2
Query: 329 LWSPTTYFASDMSTRVMILASSSFFI 252
+W P T+F ++ + I +S+ FI
Sbjct: 93 IWVPDTFFVNEKQSYFHIATTSNEFI 118
>DQ667181-1|ABG75733.1| 445|Apis mellifera GABA-gated chloride
channel protein.
Length = 445
Score = 22.2 bits (45), Expect = 3.5
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = -2
Query: 329 LWSPTTYFASDMSTRVMILASSSFFI 252
+W P T+F ++ + I +S+ FI
Sbjct: 93 IWVPDTFFVNEKQSYFHIATTSNEFI 118
>DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine
receptor alpha3subunit protein.
Length = 566
Score = 22.2 bits (45), Expect = 3.5
Identities = 7/24 (29%), Positives = 14/24 (58%)
Frame = +2
Query: 452 VLFWVAWLCMLAGAIAVIVRAPKL 523
+ W+ L ++ G +I++AP L
Sbjct: 516 LFLWIFTLAVVVGTAGIILQAPTL 539
>DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine
receptor alpha1subunit protein.
Length = 601
Score = 22.2 bits (45), Expect = 3.5
Identities = 6/24 (25%), Positives = 14/24 (58%)
Frame = +2
Query: 452 VLFWVAWLCMLAGAIAVIVRAPKL 523
+ W+ + + G + +I++AP L
Sbjct: 549 IFLWIFTVACVLGTVLIILQAPSL 572
>AF144379-1|AAD34586.1| 543|Apis mellifera glutamate transporter
Am-EAAT protein.
Length = 543
Score = 22.2 bits (45), Expect = 3.5
Identities = 14/45 (31%), Positives = 19/45 (42%)
Frame = -3
Query: 175 IRFWYSLVGCNVFILFHLPSINRETVFPWLRHR*LCTTLLKPVFH 41
I WYS G I + SIN T + + T +L +FH
Sbjct: 282 IIMWYSPFGIMCLIAGKIMSINNLTATAQMLGLYMVTVILGLLFH 326
>AF094822-1|AAC63381.1| 365|Apis mellifera GABA receptor Rdl
subunit protein.
Length = 365
Score = 22.2 bits (45), Expect = 3.5
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = -2
Query: 329 LWSPTTYFASDMSTRVMILASSSFFI 252
+W P T+F ++ + I +S+ FI
Sbjct: 32 IWVPDTFFVNEKQSYFHIATTSNEFI 57
>DQ325105-1|ABD14119.1| 180|Apis mellifera complementary sex
determiner protein.
Length = 180
Score = 21.4 bits (43), Expect = 6.0
Identities = 15/67 (22%), Positives = 25/67 (37%)
Frame = +3
Query: 84 RSHGKTVSRLMEGK*KRMNTLQPTRLYQNLIQNSGRPKRTSANRRRRYLPTAPKRNYEEG 263
R+ K SR E + + R Y+ + R KR + R + ++ NY
Sbjct: 33 RTSRKRYSRSREREQNSYKNEKEYRKYRERSKERSRDKRERERSKERKIISSLSNNYISN 92
Query: 264 RRGQNHN 284
N+N
Sbjct: 93 ISNYNNN 99
>AF388659-4|AAK71996.1| 1308|Apis mellifera NFRKB-like protein
protein.
Length = 1308
Score = 21.4 bits (43), Expect = 6.0
Identities = 12/56 (21%), Positives = 19/56 (33%)
Frame = -3
Query: 484 EHAQPGHPEQHETATNASSPRRDRQHTSVPPLSSSQ*IAVY*HRVQSSRHHSCGPR 317
+H H T N S D H + P+ S A H++ + P+
Sbjct: 225 KHESDNSDYSHTTDENRHSSTLDIDHKMLTPIKSEPIDAYEMHQISKKKLSPATPK 280
>DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channel
protein.
Length = 463
Score = 21.0 bits (42), Expect = 8.0
Identities = 7/26 (26%), Positives = 14/26 (53%)
Frame = -2
Query: 329 LWSPTTYFASDMSTRVMILASSSFFI 252
+W P TYF + + + + S + F+
Sbjct: 85 IWKPDTYFYNGKHSYLHTITSPNKFV 110
>DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein.
Length = 828
Score = 21.0 bits (42), Expect = 8.0
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2
Query: 107 SIDGGQVKEDEHVATYK 157
+I GGQ + DE+V T K
Sbjct: 292 AIAGGQQRPDENVVTDK 308
>AY352277-1|AAQ67418.1| 418|Apis mellifera complementary sex
determiner protein.
Length = 418
Score = 21.0 bits (42), Expect = 8.0
Identities = 15/67 (22%), Positives = 24/67 (35%)
Frame = +3
Query: 84 RSHGKTVSRLMEGK*KRMNTLQPTRLYQNLIQNSGRPKRTSANRRRRYLPTAPKRNYEEG 263
R+ K SR E + K R Y+ + R K + R + ++ NY
Sbjct: 271 RTSRKRYSRSREREQKSYKNENSYRKYRETSKERSRDKTERERSKERKIISSLSNNYISN 330
Query: 264 RRGQNHN 284
N+N
Sbjct: 331 ISNYNNN 337
>AF084556-1|AAC71015.1| 652|Apis mellifera pipsqueak protein.
Length = 652
Score = 21.0 bits (42), Expect = 8.0
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = -2
Query: 341 ASPFLWSPTTYFASDMSTRVMILAS 267
A+PF SPTT+ +D+ + + S
Sbjct: 510 AAPFNASPTTWSPADLDRALEAIRS 534
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 169,611
Number of Sequences: 438
Number of extensions: 3965
Number of successful extensions: 18
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18
length of database: 146,343
effective HSP length: 54
effective length of database: 122,691
effective search space used: 15213684
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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