Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0142
         (501 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   126   2e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    66   2e-12
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    65   6e-12
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    60   2e-10
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   1.6  
SPBC3H7.08c |||conserved fungal protein|Schizosaccharomyces pomb...    27   2.1  
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch...    26   3.7  
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy...    25   4.8  
SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizo...    25   6.4  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  126 bits (303), Expect = 2e-30
 Identities = 66/126 (52%), Positives = 78/126 (61%), Gaps = 4/126 (3%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 246
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 247 PRASSSTWSPAPWTMSLWTFRTDLPSGQLRXPTV----RAPATTGAKGHYTEGAELVDSV 414
           PRA      P   TM     ++       R   +           AKGHYTEGAEL D+V
Sbjct: 61  PRAVLVDLEPG--TMD--AVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116

Query: 415 *HVVSK 432
             VV +
Sbjct: 117 LDVVRR 122



 Score = 50.0 bits (114), Expect = 2e-07
 Identities = 26/63 (41%), Positives = 34/63 (53%)
 Frame = +2

Query: 293 RSGPFGQIYRPDNFVXRQYGRRQQLGPRDTTRRVLNSSIQXNTSFRKEAESCDCLQGFQL 472
           +SG FG ++RPDN +  Q G          T     +    +   R+EAE+CD LQGFQL
Sbjct: 77  KSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDV-VRREAEACDALQGFQL 135

Query: 473 THS 481
           THS
Sbjct: 136 THS 138



 Score = 33.1 bits (72), Expect = 0.024
 Identities = 19/44 (43%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDYVALDLSDRSTVRTTSYXDSTGAGNNWGQG 377
           PR +LVDLEPGTMD V                  +GAGN W +G
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG 104


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 66.5 bits (155), Expect = 2e-12
 Identities = 27/62 (43%), Positives = 43/62 (69%)
 Frame = +1

Query: 70  REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 249
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 250 RA 255
           RA
Sbjct: 63  RA 64



 Score = 29.1 bits (62), Expect = 0.39
 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 5/51 (9%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDYVALD----LSDRSTVRTTSYXDSTGAGNNWGQGTLH 386
           PR IL+DLEP  ++ +  D    L +   +  T   +  GAGNNW  G  H
Sbjct: 62  PRAILIDLEPRVVNNILSDTYGSLYNPENILITK--NGGGAGNNWANGYSH 110


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 64.9 bits (151), Expect = 6e-12
 Identities = 41/118 (34%), Positives = 54/118 (45%), Gaps = 2/118 (1%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 240
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 241 YVPRASSSTWSPAPWTMSLWTFRTDLPSGQLRXPTVRAPATTGAKGHYTEGAELVDSV 414
           YVPR+      P            DL   +         +   A+GHYT G ELVD V
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEV 118



 Score = 26.6 bits (56), Expect = 2.1
 Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = +2

Query: 293 RSGPFGQIYRPDNFVX-RQYGRRQQLGPRDTTRRVLNSSIQXNTSFRKEAESCDCLQGFQ 469
           R+GP+  ++ P+  +  ++           T  + L   +      R+ A++C  LQGF 
Sbjct: 79  RTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEV--TDKIRRIADNCSGLQGFL 136

Query: 470 LTHS 481
           + HS
Sbjct: 137 VFHS 140


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 59.7 bits (138), Expect = 2e-10
 Identities = 37/122 (30%), Positives = 54/122 (44%), Gaps = 6/122 (4%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 228
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 229 SGGKYVPRASSSTWSPAPWTMSLWTFRTDLPSGQLRXPTVRAPATTGAKGHYTEGAELVD 408
             GK+VPR+      P            DL   +         +   A+GHYT G E++D
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 409 SV 414
           SV
Sbjct: 121 SV 122



 Score = 28.7 bits (61), Expect = 0.52
 Identities = 16/65 (24%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
 Frame = +2

Query: 293 RSGPFGQIYRPDNFVX--RQYGRRQQLGPRDTTRRVLNSSIQXNTSFRKEAESCDCLQGF 466
           R+GP+  ++ P+  V            G     + +++S ++     R+ A++C  LQGF
Sbjct: 83  RTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLER---IRRMADNCSGLQGF 139

Query: 467 QLTHS 481
            + HS
Sbjct: 140 LVFHS 144


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +1

Query: 106 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 207
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPBC3H7.08c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 120

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 15/41 (36%), Positives = 20/41 (48%)
 Frame = -1

Query: 300 PERHSPWCRAPSRRGCAGHVLAAGGFIVVYIDALQLQVRVP 178
           PE    WC      G AG++L   G +V + D    QVR+P
Sbjct: 20  PELFITWCVMTYTFGVAGYMLGQRGLLVQHED----QVRIP 56


>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 867

 Score = 25.8 bits (54), Expect = 3.7
 Identities = 13/36 (36%), Positives = 20/36 (55%)
 Frame = +1

Query: 256 SSSTWSPAPWTMSLWTFRTDLPSGQLRXPTVRAPAT 363
           SS T  P+    +L ++ T +PSG  R P +  P+T
Sbjct: 347 SSVTTVPSNSPATLNSYTTSVPSGMSRHPMLMNPST 382


>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 436

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 10/24 (41%), Positives = 14/24 (58%)
 Frame = -3

Query: 403 RVQHPPCSVPWPQLLPAPVLSXYE 332
           +V  P  S+ W    PAP+L+ YE
Sbjct: 365 QVACPRLSIDWGYAFPAPLLTPYE 388


>SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 601

 Score = 25.0 bits (52), Expect = 6.4
 Identities = 11/41 (26%), Positives = 18/41 (43%)
 Frame = +1

Query: 271 SPAPWTMSLWTFRTDLPSGQLRXPTVRAPATTGAKGHYTEG 393
           +P PW  ++  +        L+ P V  P  TGA+  +  G
Sbjct: 350 APPPWLFAMQRYGPPPSYPDLKIPGVNCPIPTGAQWGFHPG 390


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,999,213
Number of Sequences: 5004
Number of extensions: 38836
Number of successful extensions: 124
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)