Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0140
(717 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_6508| Best HMM Match : AMP-binding (HMM E-Value=2.8026e-45) 110 1e-24
SB_30263| Best HMM Match : No HMM Matches (HMM E-Value=.) 79 4e-15
SB_13214| Best HMM Match : No HMM Matches (HMM E-Value=.) 79 5e-15
SB_37281| Best HMM Match : AMP-binding (HMM E-Value=3.78351e-44) 77 1e-14
SB_54338| Best HMM Match : Ligase_CoA (HMM E-Value=0) 58 7e-09
SB_31957| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.13
SB_42547| Best HMM Match : AMP-binding (HMM E-Value=1.1e-05) 31 1.2
SB_8167| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.2
SB_18399| Best HMM Match : AMP-binding (HMM E-Value=0) 30 2.2
SB_58684| Best HMM Match : AMP-binding (HMM E-Value=0.36) 29 2.8
SB_55147| Best HMM Match : TPR_2 (HMM E-Value=1.8e-10) 28 6.6
SB_6669| Best HMM Match : zf-C4_Topoisom (HMM E-Value=1.2) 28 6.6
SB_48304| Best HMM Match : AMP-binding (HMM E-Value=0) 28 8.7
>SB_6508| Best HMM Match : AMP-binding (HMM E-Value=2.8026e-45)
Length = 1038
Score = 110 bits (265), Expect = 1e-24
Identities = 48/78 (61%), Positives = 59/78 (75%)
Frame = +3
Query: 264 VWWAASDLGWVVGHSYICYGPLLAGMTSVLYEGKPDRTPDPGQYFRIIEQHRVNALFTIP 443
VWWAASDLGWVVGHSYI Y PL G T+VL+EGKP TPD G +FR+IEQH+V ++FT P
Sbjct: 329 VWWAASDLGWVVGHSYIVYAPLFNGCTTVLFEGKPVGTPDAGAFFRVIEQHKVISMFTAP 388
Query: 444 TAFRVLKRADTNAKYARR 497
TA R+++ + K A R
Sbjct: 389 TAIRIIRTERYDIKTAIR 406
Score = 87.8 bits (208), Expect = 8e-18
Identities = 47/89 (52%), Positives = 61/89 (68%), Gaps = 4/89 (4%)
Frame = +1
Query: 4 SSHQPRSCIIYQRRRVLECPLEI--GRDISWDEGLE-ADPVPCESVEANEPLYILYTSGT 174
SS +P + ++YQR +C +I GRDI+WD+ +E A+P C V A +PLYILYTSGT
Sbjct: 240 SSFKPSTVVLYQRD---QCTGDIIPGRDITWDQVMERAEPHDCVPVLATDPLYILYTSGT 296
Query: 175 TDAPKG-VQRPCGHAATLCWSMKKVYGLN 258
T PKG V+R GHA L WSMK +YG+N
Sbjct: 297 TGDPKGIVRRNGGHAVALNWSMKNIYGVN 325
>SB_30263| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 976
Score = 79.0 bits (186), Expect = 4e-15
Identities = 35/84 (41%), Positives = 54/84 (64%), Gaps = 2/84 (2%)
Frame = +3
Query: 249 WSQRXVWWAASDLGWVVGHSYICYGPLLAGMTSVLYEGKPDRTPDPGQYFRIIEQHRVNA 428
+ Q V+ +D+GW+ GHSY+ YGPL G T+VL+E P PDPG+Y+ ++E+ R+N
Sbjct: 303 YKQGDVFGCVADIGWITGHSYVVYGPLANGATTVLFESTP-TYPDPGRYWEMVERLRINQ 361
Query: 429 LFTIPTAFRVLKRADTN--AKYAR 494
+ PTA R+L ++ + KY R
Sbjct: 362 FYGAPTAIRLLLKSGNSWVEKYDR 385
Score = 40.3 bits (90), Expect = 0.002
Identities = 17/41 (41%), Positives = 27/41 (65%), Gaps = 2/41 (4%)
Frame = +1
Query: 79 DISWDEGLEADPVPC--ESVEANEPLYILYTSGTTDAPKGV 195
DI +E + P C E +++ +PL++LYTSG+T PKG+
Sbjct: 242 DIPLEEAMAEQPDECPPEPMDSEDPLFLLYTSGSTGQPKGI 282
>SB_13214| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 84
Score = 78.6 bits (185), Expect = 5e-15
Identities = 32/74 (43%), Positives = 50/74 (67%)
Frame = +3
Query: 249 WSQRXVWWAASDLGWVVGHSYICYGPLLAGMTSVLYEGKPDRTPDPGQYFRIIEQHRVNA 428
+ Q V+ +D+GW+ GHSY+ YGPL G T+VL+E P PDPG+Y+ ++E+ R+N
Sbjct: 5 YKQGDVFGCVADIGWITGHSYVVYGPLANGATTVLFESTP-TYPDPGRYWEMVERLRINQ 63
Query: 429 LFTIPTAFRVLKRA 470
+ PTA R+L ++
Sbjct: 64 FYGAPTAIRLLLKS 77
>SB_37281| Best HMM Match : AMP-binding (HMM E-Value=3.78351e-44)
Length = 393
Score = 77.0 bits (181), Expect = 1e-14
Identities = 31/68 (45%), Positives = 48/68 (70%)
Frame = +3
Query: 264 VWWAASDLGWVVGHSYICYGPLLAGMTSVLYEGKPDRTPDPGQYFRIIEQHRVNALFTIP 443
V+W +D+GW+ GHSYI YGP+ G SVL+EG P PD G+++ I+++++V+ +T P
Sbjct: 60 VYWCTADIGWITGHSYITYGPMANGAISVLFEGTP-FYPDCGRFWDIVDKYKVSKFYTAP 118
Query: 444 TAFRVLKR 467
TA R L +
Sbjct: 119 TAIRSLMK 126
Score = 35.9 bits (79), Expect = 0.033
Identities = 14/22 (63%), Positives = 19/22 (86%)
Frame = +1
Query: 130 VEANEPLYILYTSGTTDAPKGV 195
V+A +PL++LYTSG+T PKGV
Sbjct: 13 VDAEDPLFMLYTSGSTGKPKGV 34
>SB_54338| Best HMM Match : Ligase_CoA (HMM E-Value=0)
Length = 445
Score = 58.0 bits (134), Expect = 7e-09
Identities = 22/47 (46%), Positives = 35/47 (74%)
Frame = +3
Query: 360 GKPDRTPDPGQYFRIIEQHRVNALFTIPTAFRVLKRADTNAKYARRY 500
GKP RTPD G ++R+ E+++VNA+F PTAFR +++ D + A++Y
Sbjct: 185 GKPVRTPDAGAFWRMCEEYKVNAIFAAPTAFRAIRKEDPQCELAKQY 231
>SB_31957| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 538
Score = 33.9 bits (74), Expect = 0.13
Identities = 13/26 (50%), Positives = 20/26 (76%)
Frame = +1
Query: 130 VEANEPLYILYTSGTTDAPKGVQRPC 207
V+ ++P+ I+YTSG+T +PK VQ C
Sbjct: 164 VQIDDPIVIVYTSGSTGSPKLVQHTC 189
>SB_42547| Best HMM Match : AMP-binding (HMM E-Value=1.1e-05)
Length = 529
Score = 30.7 bits (66), Expect = 1.2
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = +1
Query: 139 NEPLYILYTSGTTDAPKGVQRPCGHAATL 225
N PL+I+Y+SGTT PK + G + L
Sbjct: 274 NHPLFIMYSSGTTGPPKCMVHSAGDSNRL 302
>SB_8167| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 84
Score = 29.9 bits (64), Expect = 2.2
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +1
Query: 130 VEANEPLYILYTSGTTDAPKGVQ 198
V ++ YI+YTSG+T PKGV+
Sbjct: 28 VPSSHLCYIIYTSGSTGQPKGVE 50
>SB_18399| Best HMM Match : AMP-binding (HMM E-Value=0)
Length = 1381
Score = 29.9 bits (64), Expect = 2.2
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +1
Query: 130 VEANEPLYILYTSGTTDAPKGVQ 198
V ++ YI+YTSG+T PKGV+
Sbjct: 176 VPSSHLCYIIYTSGSTGQPKGVE 198
>SB_58684| Best HMM Match : AMP-binding (HMM E-Value=0.36)
Length = 237
Score = 29.5 bits (63), Expect = 2.8
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +1
Query: 151 YILYTSGTTDAPKGV 195
YILY+SGTT PKGV
Sbjct: 145 YILYSSGTTGLPKGV 159
>SB_55147| Best HMM Match : TPR_2 (HMM E-Value=1.8e-10)
Length = 559
Score = 28.3 bits (60), Expect = 6.6
Identities = 21/63 (33%), Positives = 27/63 (42%), Gaps = 1/63 (1%)
Frame = -3
Query: 388 PGSGVRSGFPSYRTEVIPAKSGP*QMYE*PTTQPRSDAAHHTXR*DHTPFSWTSRAWL-R 212
PG G G P P P Y T+ R D++H T R T S+ S + + R
Sbjct: 284 PGQGFPPGAPPGSGGFPPGSGFPPVEYPKNYTRRRDDSSHSTLRRSSTDHSFGSYSTVTR 343
Query: 211 GRR 203
GRR
Sbjct: 344 GRR 346
>SB_6669| Best HMM Match : zf-C4_Topoisom (HMM E-Value=1.2)
Length = 523
Score = 28.3 bits (60), Expect = 6.6
Identities = 24/75 (32%), Positives = 34/75 (45%), Gaps = 10/75 (13%)
Frame = -2
Query: 710 SSRXLGAGHGNLNARKPKARVASDRG------TPDSRFAHQ*IE----VLARRRHVQPHC 561
S+R AG G +RK + V+ RG TP++ AH + A R H
Sbjct: 96 SARMYLAGQGG--SRKAEWAVSMFRGRNVVVLTPENDLAHDHRDNPRLAYAGRAQTYHHY 153
Query: 560 PCIPGHNALQQ*RPS 516
PCIP +++ RPS
Sbjct: 154 PCIPAEKPIEEWRPS 168
>SB_48304| Best HMM Match : AMP-binding (HMM E-Value=0)
Length = 374
Score = 27.9 bits (59), Expect = 8.7
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +1
Query: 67 EIGRDISWDEGLEADPVPCESVEANEPLYILYTSGTTDAPKGVQR 201
E +D+ D+G + C S + E + Y+SGTT KGV +
Sbjct: 111 EFFKDVLEDDGSALTGIYCPS-DTTETACLPYSSGTTGLSKGVMQ 154
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 26,192,527
Number of Sequences: 59808
Number of extensions: 627224
Number of successful extensions: 1778
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1576
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1773
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1901817086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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