BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0126 (692 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 28 5.1 At4g24490.1 68417.m03510 geranylgeranyl transferase alpha subuni... 28 6.8 At3g57660.1 68416.m06424 DNA-directed RNA polymerase family prot... 27 8.9 >At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam profile: PF00225 Kinesin motor domain; contains non-consensus splice site (GC) at intron 12 Length = 2722 Score = 28.3 bits (60), Expect = 5.1 Identities = 15/41 (36%), Positives = 28/41 (68%), Gaps = 2/41 (4%) Frame = +2 Query: 443 KYILLKVYDQEV-DGVEPSTIVLEDIRDGNYHSDIYD-EVQ 559 K L++Y++++ D +EPS+ L+DI+ G Y ++ + EVQ Sbjct: 328 KCSFLEIYNEQITDLLEPSSTNLQDIKSGVYVENLTECEVQ 368 >At4g24490.1 68417.m03510 geranylgeranyl transferase alpha subunit-related / RAB geranylgeranyltransferase alpha subunit-related low similarity to SP|Q08602 [Rattus norvegicus] Length = 683 Score = 27.9 bits (59), Expect = 6.8 Identities = 22/66 (33%), Positives = 29/66 (43%) Frame = +2 Query: 332 TKFWNPNLSGPILQFTTKMSGSLHKVPKVDIDKSGVFKYILLKVYDQEVDGVEPSTIVLE 511 T W + S IL +SGS +SG F IL +DQ V GV ST+ ++ Sbjct: 242 TSSWPSHGSSIILSGAGCLSGSSSMFTTF-CSESGSFPLILY--FDQAVGGVSSSTVTID 298 Query: 512 DIRDGN 529 GN Sbjct: 299 SELKGN 304 >At3g57660.1 68416.m06424 DNA-directed RNA polymerase family protein similar to SP|O35134 DNA-directed RNA polymerase I largest subunit (EC 2.7.7.6) (RNA polymerase I 194 kDa subunit) (RPA194) {Mus musculus}; contains InterPro accession IPR000722: RNA polymerase, alpha subunit Length = 1670 Score = 27.5 bits (58), Expect = 8.9 Identities = 19/69 (27%), Positives = 28/69 (40%) Frame = +2 Query: 347 PNLSGPILQFTTKMSGSLHKVPKVDIDKSGVFKYILLKVYDQEVDGVEPSTIVLEDIRDG 526 P L G + ++ L K+ DI KS + VY+ EV + I L Sbjct: 1214 PLLKGKTKEDANDITDRLRKITVADIIKSMELSVVPYTVYENEVCSIHKLKINLYKPEHY 1273 Query: 527 NYHSDIYDE 553 H+DI +E Sbjct: 1274 PKHTDITEE 1282 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,957,193 Number of Sequences: 28952 Number of extensions: 350342 Number of successful extensions: 832 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 809 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 832 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1477286152 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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