BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0125 (720 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 142 3e-35 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 135 7e-33 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 121 9e-29 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 120 2e-28 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 86 4e-18 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 51 1e-07 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 1e-04 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 30 0.29 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.38 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.88 SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subuni... 28 1.5 SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 27 3.6 SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 3.6 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 6.2 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 8.2 SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co... 25 8.2 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 142 bits (345), Expect = 3e-35 Identities = 67/100 (67%), Positives = 79/100 (79%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P +TIFDAKRLIGRKF+D VQ+DMKHWP V+S GKP ++V YKG KTF PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 VL KM+ETAEAYLG V +AV+TVPAY + +ATKDAG Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAG 160 Score = 112 bits (270), Expect = 4e-26 Identities = 51/59 (86%), Positives = 56/59 (94%) Frame = +3 Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPN 254 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+ Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPH 62 Score = 38.7 bits (86), Expect = 8e-04 Identities = 28/56 (50%), Positives = 30/56 (53%) Frame = +1 Query: 511 NDSQRQGHKRCR*PSLA*TFLRIINEPTAAAIAYGL*TKKGTWRTKLYLSFDLRAG 678 NDSQRQ K + LRIINEPTAAAIAYGL + L FDL G Sbjct: 149 NDSQRQATKDAGLIA-GLNVLRIINEPTAAAIAYGL--DRSNQGESNVLIFDLGGG 201 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 135 bits (326), Expect = 7e-33 Identities = 63/100 (63%), Positives = 77/100 (77%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P +TIFDAKRLIGR+F D VQ+DMKHWP V+ GKP I+V +KG KTF PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 VL KM+E+AEA+LG V +AV+TVPAY + +ATKDAG Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAG 160 Score = 112 bits (270), Expect = 4e-26 Identities = 51/59 (86%), Positives = 56/59 (94%) Frame = +3 Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPN 254 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+ Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPH 62 Score = 39.9 bits (89), Expect = 4e-04 Identities = 29/56 (51%), Positives = 32/56 (57%) Frame = +1 Query: 511 NDSQRQGHKRCR*PSLA*TFLRIINEPTAAAIAYGL*TKKGTWRTKLYLSFDLRAG 678 NDSQRQ K + LRIINEPTAAAIAYGL + T + L FDL G Sbjct: 149 NDSQRQATKDAGLIA-GLNVLRIINEPTAAAIAYGL-DRSNQHETNV-LIFDLGGG 201 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 121 bits (292), Expect = 9e-29 Identities = 57/100 (57%), Positives = 72/100 (72%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P++T+FDAKRLIGR++ED Q D+KHWP V+ + G P I+V Y G K F +E+S+M Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 VLTKMKE +EA L K V+ AVITVPAY + ATKDAG Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAG 164 Score = 100 bits (240), Expect = 2e-22 Identities = 47/63 (74%), Positives = 54/63 (85%), Gaps = 1/63 (1%) Frame = +3 Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP-N 254 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67 Query: 255 TQY 263 T + Sbjct: 68 TVF 70 Score = 39.5 bits (88), Expect = 5e-04 Identities = 23/36 (63%), Positives = 24/36 (66%) Frame = +1 Query: 571 LRIINEPTAAAIAYGL*TKKGTWRTKLYLSFDLRAG 678 LRIINEPTAAAIAYGL K + K L FDL G Sbjct: 172 LRIINEPTAAAIAYGLDAKSD--KPKNVLIFDLGGG 205 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 120 bits (289), Expect = 2e-28 Identities = 55/100 (55%), Positives = 76/100 (76%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P++TIFD KRLIGRKF++ T+ D+K +P +V+D +P ++V G K F PEE+S+M Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 +L+KMK+TAEAYLGK V +AV+TVPAY + +ATKDAG Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAG 193 Score = 98.7 bits (235), Expect = 7e-22 Identities = 45/64 (70%), Positives = 54/64 (84%), Gaps = 1/64 (1%) Frame = +3 Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP-NT 257 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP NT Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97 Query: 258 QYSM 269 + + Sbjct: 98 IFDI 101 Score = 36.7 bits (81), Expect = 0.003 Identities = 20/36 (55%), Positives = 23/36 (63%) Frame = +1 Query: 511 NDSQRQGHKRCR*PSLA*TFLRIINEPTAAAIAYGL 618 ND+QRQ K +RI+NEPTAAAIAYGL Sbjct: 182 NDAQRQATKDAG-TIAGLNVIRIVNEPTAAAIAYGL 216 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 86.2 bits (204), Expect = 4e-18 Identities = 45/100 (45%), Positives = 63/100 (63%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P++T F KRLIGR+F++ VQ D+K P +V A +G KT+ P ++ Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGDAWLEA-RG--KTYSPSQIGGF 166 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 +L+KM+ETA YLGK V+NAV+TVPAY + +ATK AG Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAG 206 Score = 73.7 bits (173), Expect = 2e-14 Identities = 34/66 (51%), Positives = 45/66 (68%), Gaps = 1/66 (1%) Frame = +3 Query: 69 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 245 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q + Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 246 NPNTQY 263 NP + Sbjct: 109 NPENTF 114 Score = 37.9 bits (84), Expect = 0.001 Identities = 20/36 (55%), Positives = 23/36 (63%) Frame = +1 Query: 511 NDSQRQGHKRCR*PSLA*TFLRIINEPTAAAIAYGL 618 NDSQRQ K LR++NEPTAAA+AYGL Sbjct: 195 NDSQRQATKAAG-AIAGLNVLRVVNEPTAAALAYGL 229 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 51.2 bits (117), Expect = 1e-07 Identities = 21/61 (34%), Positives = 39/61 (63%) Frame = +3 Query: 66 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 245 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62 Query: 246 N 248 N Sbjct: 63 N 63 Score = 43.2 bits (97), Expect = 4e-05 Identities = 27/101 (26%), Positives = 53/101 (52%), Gaps = 1/101 (0%) Frame = +2 Query: 251 QHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSD-GGKPKIKVAYKGXDKTFFPEEVSSM 427 ++T+ KRL GR ++D ++ ++ +++ G KV Y + F ++ + Sbjct: 65 RNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAA 124 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAGN 550 TK+K AEA L +V + VI++PA+ ++ +A +A N Sbjct: 125 YFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAAN 165 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/56 (30%), Positives = 31/56 (55%) Frame = +3 Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 248 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRN 82 Score = 35.5 bits (78), Expect = 0.008 Identities = 19/57 (33%), Positives = 28/57 (49%) Frame = +2 Query: 392 DKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAGNHLWL 562 +K E S L ++ E+AE +LG V V++VP Y + KA + A N L Sbjct: 135 EKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGL 191 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 30.3 bits (65), Expect = 0.29 Identities = 34/138 (24%), Positives = 60/138 (43%) Frame = -1 Query: 648 FRSPSTLFCSKTVSNRSSSRFIDDSEKRSSQRWLPASFVALSLRVIEYAGTVITAFCTVL 469 F S S++ S T S S+S I S S+ F + S + I++ T+L Sbjct: 573 FSSVSSILSSSTSSPSSTSLSISSSSTSST-------FSSASTSSPSSISSSISSSSTIL 625 Query: 468 PR*ASAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTXKGQCFMSACTVASSNL 289 + S + +S+ + S +LSS + + + SL+T S+ T+ SS+ Sbjct: 626 SSPTPSTSSLMISSSSIISGSSSILSSSIST---IPISSSLSTYSSSVIPSSSTLVSSSS 682 Query: 288 RPMRRLASNIVCWGSSPP 235 + ++S+ V SS P Sbjct: 683 SLI--VSSSPVASSSSSP 698 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 29.9 bits (64), Expect = 0.38 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%) Frame = +3 Query: 81 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNT 257 + ID GT ++ + + G +EI+ R S VAF ER+ G A N P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82 Query: 258 QYSMPNV 278 +S+ NV Sbjct: 83 AHSIRNV 89 Score = 27.1 bits (57), Expect = 2.7 Identities = 22/105 (20%), Positives = 44/105 (41%), Gaps = 2/105 (1%) Frame = +2 Query: 248 PQHTIFDAKRLIGRK-FEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFP-EEVS 421 P H+I + K L+ E VQ +P + + + +++ D+ + EE+ Sbjct: 82 PAHSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVISDEENYSLEEII 141 Query: 422 SMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAGNHL 556 +M + AE + + + V+TVP + L+ +A L Sbjct: 142 AMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARIL 186 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.88 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +1 Query: 73 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 168 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subunit Cwg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 355 Score = 27.9 bits (59), Expect = 1.5 Identities = 13/49 (26%), Positives = 25/49 (51%) Frame = -1 Query: 645 RSPSTLFCSKTVSNRSSSRFIDDSEKRSSQRWLPASFVALSLRVIEYAG 499 R+ FC + ++ IDD +K+S W+ ++V + I+Y+G Sbjct: 30 RTVLAFFCLLGLDLLNALNTIDDDDKKSWIEWIYKNYVTKESKGIKYSG 78 >SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 26.6 bits (56), Expect = 3.6 Identities = 13/40 (32%), Positives = 19/40 (47%), Gaps = 2/40 (5%) Frame = +1 Query: 430 AYENEGNCRSLSWQNCAECSYHGSRV--LNDSQRQGHKRC 543 +Y N R L W C + GSR+ + ++ Q KRC Sbjct: 266 SYRMSSNVRPLIWVECESIVHSGSRIEFMVKAKAQFKKRC 305 >SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 2244 Score = 26.6 bits (56), Expect = 3.6 Identities = 14/45 (31%), Positives = 24/45 (53%) Frame = +3 Query: 177 PSYVAFTDTERLIGDAAKNQVAMNPNTQYSMPNVSSDVSSKMLLC 311 P +A T ER++ AAK A+N Y++ ++ D K++ C Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.8 bits (54), Expect = 6.2 Identities = 16/53 (30%), Positives = 26/53 (49%) Frame = -1 Query: 159 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 1 G W SP S + H ++++ +F +RVL C+ L K +K+A L Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 25.4 bits (53), Expect = 8.2 Identities = 14/35 (40%), Positives = 18/35 (51%) Frame = +1 Query: 427 GAYENEGNCRSLSWQNCAECSYHGSRVLNDSQRQG 531 GAY N C +S N A CS S ++ND + G Sbjct: 1689 GAYSNA--CVDISLSNLALCSILNSALINDIEGIG 1721 >SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 25.4 bits (53), Expect = 8.2 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = +3 Query: 222 AAKNQVAMNPNTQYSMPNVSSDVSSKML 305 A K Q+ +NP + +PNV D + +L Sbjct: 323 ARKRQIQLNPFPPWDVPNVLEDQMNSLL 350 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,049,803 Number of Sequences: 5004 Number of extensions: 64375 Number of successful extensions: 217 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 191 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 215 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 337208592 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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