BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0125 (720 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 145 3e-35 SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 143 1e-34 SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 130 1e-30 SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 122 2e-28 SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 70 2e-12 SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 67 2e-11 SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 63 3e-10 SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 4e-04 SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 37 0.014 SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.94 SB_13698| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.2 SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.9 SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 5.0 SB_33568| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.0 SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.0 SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.6 SB_25409| Best HMM Match : PSD1 (HMM E-Value=7.2) 28 8.8 SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14) 28 8.8 >SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) Length = 640 Score = 145 bits (352), Expect = 3e-35 Identities = 81/151 (53%), Positives = 99/151 (65%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P++TIFDAKRLIGR+++DATVQ+DMK WP ++SD KPKI+V YKG KTF EE+SSM Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAGNHLWLERFSESSMNRLLLRL 607 VL KMKETAEAYLG+ V +AVITVPAY + +ATKDAG L +N Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVL--RVINEPTAAA 180 Query: 608 LTVFEQKRVLGERNCTYLLTFGRAGYLSDVS 700 L K + GE+N +L F G DVS Sbjct: 181 LAYGLDKSLSGEKN---VLIFDLGGGTFDVS 208 Score = 127 bits (306), Expect = 1e-29 Identities = 57/63 (90%), Positives = 61/63 (96%) Frame = +3 Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242 M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60 Query: 243 MNP 251 +NP Sbjct: 61 LNP 63 >SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1097 Score = 143 bits (346), Expect = 1e-34 Identities = 65/100 (65%), Positives = 80/100 (80%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P++T+FDAKRLIGRKF+D V DM HWP V+ +G +PKI+V +KG K+FFPEE+SSM Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 VLTKMKETAEAYLG V +AV+TVPAY + +ATKDAG Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAG 162 Score = 132 bits (319), Expect = 3e-31 Identities = 63/68 (92%), Positives = 65/68 (95%), Gaps = 1/68 (1%) Frame = +3 Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242 MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60 Query: 243 MNP-NTQY 263 MNP NT + Sbjct: 61 MNPENTVF 68 Score = 41.5 bits (93), Expect = 7e-04 Identities = 29/56 (51%), Positives = 31/56 (55%) Frame = +1 Query: 511 NDSQRQGHKRCR*PSLA*TFLRIINEPTAAAIAYGL*TKKGTWRTKLYLSFDLRAG 678 +DSQRQ K LRIINEPTAAAIAYGL K G R L +DL G Sbjct: 151 HDSQRQATKDAG-TIAGLNVLRIINEPTAAAIAYGLDKKVGVERN--VLIYDLGGG 203 >SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 746 Score = 130 bits (314), Expect = 1e-30 Identities = 59/63 (93%), Positives = 61/63 (96%) Frame = +3 Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242 M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60 Query: 243 MNP 251 MNP Sbjct: 61 MNP 63 Score = 117 bits (281), Expect = 1e-26 Identities = 67/125 (53%), Positives = 79/125 (63%), Gaps = 25/125 (20%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEV--- 418 P +TIFDAKRLIGR+F+D V+ DMKHW VV++ G+PK+KV YKG KTFF EEV Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122 Query: 419 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKA 532 SSMVL KMKETAEAYLG V +AV+TVPAY + +A Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQA 182 Query: 533 TKDAG 547 TKDAG Sbjct: 183 TKDAG 187 Score = 61.3 bits (142), Expect = 8e-10 Identities = 30/45 (66%), Positives = 36/45 (80%) Frame = +2 Query: 413 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 ++SSMVL KMKETAEAYLG V +AV+TVPAY + +ATKDAG Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAG 255 Score = 44.8 bits (101), Expect = 7e-05 Identities = 32/56 (57%), Positives = 32/56 (57%) Frame = +1 Query: 511 NDSQRQGHKRCR*PSLA*TFLRIINEPTAAAIAYGL*TKKGTWRTKLYLSFDLRAG 678 NDSQRQ K S LRIINEPTAAAIAYGL K G R L FDL G Sbjct: 244 NDSQRQATKDAGVIS-GLNVLRIINEPTAAAIAYGLDKKVGQERN--VLIFDLGGG 296 >SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1327 Score = 122 bits (295), Expect = 2e-28 Identities = 59/100 (59%), Positives = 75/100 (75%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P++T+FDAKRLIGR ++D +VQ D+K +P VV KP ++V K KTF EE+S+M Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 VLTKMKETAEAYLGK V +AV+TVPAY + +ATKDAG Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAG 860 Score = 100 bits (239), Expect = 1e-21 Identities = 48/63 (76%), Positives = 55/63 (87%), Gaps = 2/63 (3%) Frame = +3 Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNP-N 254 +GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763 Query: 255 TQY 263 T + Sbjct: 764 TVF 766 Score = 44.0 bits (99), Expect = 1e-04 Identities = 29/56 (51%), Positives = 33/56 (58%) Frame = +1 Query: 511 NDSQRQGHKRCR*PSLA*TFLRIINEPTAAAIAYGL*TKKGTWRTKLYLSFDLRAG 678 ND+QRQ K T +RIINEPTAAAIAYG+ K+G K L FDL G Sbjct: 849 NDAQRQATKDAG-AIAGLTVMRIINEPTAAAIAYGMDKKEG---EKNILVFDLGGG 900 >SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1068 Score = 70.1 bits (164), Expect = 2e-12 Identities = 39/99 (39%), Positives = 55/99 (55%), Gaps = 1/99 (1%) Frame = +2 Query: 251 QHTIFDAKRLIGRKFEDATVQADMKHWPLXVVS-DGGKPKIKVAYKGXDKTFFPEEVSSM 427 ++TI KR IGRKF D VQ ++ H P VV I+V Y G + F PE+V +M Sbjct: 120 KNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAM 179 Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDA 544 + T++K TAE L V + VI+VP+Y + + DA Sbjct: 180 LFTRLKTTAEIALKTKVTDCVISVPSYYTDRQRRCMLDA 218 Score = 49.6 bits (113), Expect = 3e-06 Identities = 22/52 (42%), Positives = 33/52 (63%) Frame = +3 Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 236 VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55 >SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) Length = 437 Score = 66.9 bits (156), Expect = 2e-11 Identities = 32/68 (47%), Positives = 46/68 (67%), Gaps = 3/68 (4%) Frame = +3 Query: 69 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242 K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89 Query: 243 MNP-NTQY 263 +NP NT Y Sbjct: 90 INPKNTIY 97 Score = 54.0 bits (124), Expect = 1e-07 Identities = 32/87 (36%), Positives = 45/87 (51%), Gaps = 1/87 (1%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDG-GKPKIKVAYKGXDKTFFPEEVSS 424 P++TI+DAKR IG+ F + K + V +D G V KG PEE+ Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151 Query: 425 MVLTKMKETAEAYLGKTVQNAVITVPA 505 V+ K++ AE L TV AV++VPA Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPA 178 Score = 38.3 bits (85), Expect = 0.006 Identities = 17/23 (73%), Positives = 20/23 (86%) Frame = +1 Query: 565 TFLRIINEPTAAAIAYGL*TKKG 633 T LR+INEPTAAA+AYGL K+G Sbjct: 198 TVLRVINEPTAAALAYGLHKKEG 220 >SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) Length = 179 Score = 62.9 bits (146), Expect = 3e-10 Identities = 32/75 (42%), Positives = 49/75 (65%) Frame = +2 Query: 368 IKVAYKGXDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAG 547 I V G ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+ + +ATKDAG Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAG 61 Query: 548 NHLWLERFSESSMNR 592 L+ S ++ R Sbjct: 62 TIAGLDVLSRTASER 76 Score = 35.9 bits (79), Expect = 0.033 Identities = 17/21 (80%), Positives = 18/21 (85%) Frame = +1 Query: 571 LRIINEPTAAAIAYGL*TKKG 633 LRIINEPTAAAIAYGL +G Sbjct: 93 LRIINEPTAAAIAYGLDKARG 113 >SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) Length = 592 Score = 42.3 bits (95), Expect = 4e-04 Identities = 27/61 (44%), Positives = 34/61 (55%), Gaps = 3/61 (4%) Frame = +3 Query: 132 GKVEIIANDQGNRTTPSYVAFTDTERLI--GDAAKNQVAMNP-NTQYSMPNVSSDVSSKM 302 G IIAND G+R TP+ VAF+DTE+LI +V N N Q S V + + KM Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLIDKDGLPYYEVESNERNIQVSPKEVINMIYKKM 142 Query: 303 L 305 L Sbjct: 143 L 143 >SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1019 Score = 37.1 bits (82), Expect = 0.014 Identities = 23/89 (25%), Positives = 41/89 (46%) Frame = +2 Query: 362 PKIKVAYKGXDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKD 541 P +K Y+ D TF PEE+ M+L + E + +++ V+TVP + + +A Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLR 126 Query: 542 AGNHLWLERFSESSMNRLLLRLLTVFEQK 628 A + L + N + +F+QK Sbjct: 127 AAELVGLNVLQIMNSNTAVALNYGLFQQK 155 >SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) Length = 212 Score = 31.1 bits (67), Expect = 0.94 Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%) Frame = +3 Query: 141 EIIANDQGNRTTPSYVAFT-DTERL 212 ++I N +G+RTTPS VAFT + ERL Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31 >SB_13698| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 358 Score = 29.9 bits (64), Expect = 2.2 Identities = 31/100 (31%), Positives = 45/100 (45%), Gaps = 11/100 (11%) Frame = -1 Query: 630 LFCSKTVSNRSSSRFIDDSEKRSSQRWLP-ASFVALSLRVIEY-AGTVIT-------AFC 478 + S TV +S+R+I+ S S + P S L+L I Y G + T AF Sbjct: 1 MLISNTVMEANSTRYINTSASDRSYTFTPLTSQTKLTLTAIIYLVGVIATIGNIGIIAFR 60 Query: 477 TVLP--R*ASAVSFIFVSTMELTSSGKKVLSSPLYATLIL 364 L R +F FV ++ + G VLS PLY ++L Sbjct: 61 HALNNRRLQRNRTFYFVQSLAWSDLGASVLSLPLYNAIML 100 >SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) Length = 350 Score = 29.5 bits (63), Expect = 2.9 Identities = 16/33 (48%), Positives = 19/33 (57%) Frame = -1 Query: 249 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 151 GS PPG W R +AR T +T +SC PG W Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236 >SB_9053| Best HMM Match : TIG (HMM E-Value=0) Length = 2990 Score = 28.7 bits (61), Expect = 5.0 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = +3 Query: 9 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 95 HVC +V T K K ++K PA GI+L Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228 >SB_33568| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1555 Score = 28.7 bits (61), Expect = 5.0 Identities = 17/66 (25%), Positives = 32/66 (48%) Frame = +2 Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPLXVVSDGGKPKIKVAYKGXDKTFFPEEVSSM 427 P H I A R++GRK +++++D + V KP + K F P+ ++S+ Sbjct: 572 PSHEIAQAWRILGRKSSRSSLESDTLE--IKVDPKDSKPTKRNILKQVASVFDPQGLASL 629 Query: 428 VLTKMK 445 + + K Sbjct: 630 FVLRAK 635 >SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1506 Score = 28.7 bits (61), Expect = 5.0 Identities = 17/58 (29%), Positives = 27/58 (46%) Frame = -1 Query: 456 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTXKGQCFMSACTVASSNLRP 283 S F+F ST + +S +KV+ SPL + + P+ G S C V + + P Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNVQNLSRCP 1165 >SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 665 Score = 28.3 bits (60), Expect = 6.6 Identities = 20/75 (26%), Positives = 36/75 (48%), Gaps = 1/75 (1%) Frame = +2 Query: 50 LKIQNGKSTRSRN-RSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCR 226 L++ N + ++ + R GY L R C R + +R + H++L R +RC Sbjct: 225 LRLVNTRDDKTHDKRPGYRKLSRVC-GGRRPNEPYERTARV-HTILSTETETRFCAKRCA 282 Query: 227 QEPGGDEPQHTIFDA 271 G +P++T F+A Sbjct: 283 GTASGMDPKNTPFNA 297 >SB_25409| Best HMM Match : PSD1 (HMM E-Value=7.2) Length = 889 Score = 27.9 bits (59), Expect = 8.8 Identities = 15/55 (27%), Positives = 24/55 (43%) Frame = +2 Query: 392 DKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKATKDAGNHL 556 D F + ++ K+KE Y+ K +V ++ TLK K K+A L Sbjct: 705 DNKEFTSRLDPQLIQKLKEKHIRYIHKLPDESVTNYVSWQQTLKTKDKKEAEEFL 759 >SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14) Length = 856 Score = 27.9 bits (59), Expect = 8.8 Identities = 17/60 (28%), Positives = 27/60 (45%) Frame = -3 Query: 259 CVLGFIATWFLAASPMRRSVSVNAT*DGVVLLPWSLAMISTFPCWKTPTQEYVVPRSIPT 80 C+L I WF P+R S + VV+ S+ ++ F C P + P +IP+ Sbjct: 107 CLLLTIERWFAVVQPLRYQASFSKR--RVVVSLISVYCVACFLCCTLPFEVSFSPENIPS 164 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 23,842,649 Number of Sequences: 59808 Number of extensions: 538844 Number of successful extensions: 1469 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 1359 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1461 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1913853903 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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