Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0109
(697 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 151 9e-38
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 62 1e-10
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 60 2e-10
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 59 5e-10
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 38 0.001
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 33 0.039
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 29 0.64
SPBC1271.12 |kes1||oxysterol binding protein |Schizosaccharomyce... 29 0.84
SPCC191.11 |inv1||beta-fructofuranosidase|Schizosaccharomyces po... 28 1.1
SPAC10F6.11c |||kinase activator |Schizosaccharomyces pombe|chr ... 27 3.4
SPCC11E10.04 |||mitochondrial ATPase expression protein homolog|... 26 4.5
SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosa... 26 4.5
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 26 4.5
SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S... 26 5.9
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ... 26 5.9
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 5.9
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 25 7.8
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 151 bits (366), Expect = 9e-38
Identities = 82/159 (51%), Positives = 107/159 (67%), Gaps = 1/159 (0%)
Frame = +2
Query: 182 MEKVGQALVSGT*QCREDNTLTYVEDDRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQQ 361
+ K + L G + L +++DRLA PTLHPTSEEL+IG++RFTTFDLGGHQQ
Sbjct: 17 VNKHAKMLFLGLDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQ 76
Query: 362 ARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCPVLFSATRLINPVL 541
ARR+WRDYFP V+ IV+LVD CD RL ESKAELD+LL E L+ P L ++ P
Sbjct: 77 ARRLWRDYFPEVNGIVYLVDCCDFERLSESKAELDALLAMEELARVPFLILGNKIDAPG- 135
Query: 542 PVKTNYVTFSILYQQTTGKGKVSRSELPG-RPLELFMCS 655
+ + + ++ QTTGKG VS+ +PG RP+E+FMCS
Sbjct: 136 AISEDELKAALGLYQTTGKG-VSK-PVPGIRPIEVFMCS 172
Score = 64.1 bits (149), Expect = 2e-11
Identities = 29/41 (70%), Positives = 32/41 (78%)
Frame = +3
Query: 135 MFILDWFTGVLGFLGLWKKSGKLLFLGLDNAGKTTLLHMLK 257
MFI++WF L LGL K K+LFLGLDNAGKTTLLHMLK
Sbjct: 1 MFIINWFYDALAMLGLVNKHAKMLFLGLDNAGKTTLLHMLK 41
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 61.7 bits (143), Expect = 1e-10
Identities = 24/82 (29%), Positives = 49/82 (59%)
Frame = +2
Query: 278 VPTLHPTSEELSIGSMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKA 457
+PT+ E ++ +++F +D+GG + R +WR YF ++F+VD+ D R+ E++
Sbjct: 50 IPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDSADSNRISEARQ 109
Query: 458 ELDSLLTDETLSNCPVLFSATR 523
EL +++D + +C +L A +
Sbjct: 110 ELHRIISDREMRDCLLLVLANK 131
Score = 32.7 bits (71), Expect = 0.052
Identities = 14/24 (58%), Positives = 19/24 (79%)
Frame = +3
Query: 189 KSGKLLFLGLDNAGKTTLLHMLKM 260
K ++L LGLD AGKTT+L+ LK+
Sbjct: 20 KEMRILMLGLDAAGKTTILYKLKL 43
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 60.5 bits (140), Expect = 2e-10
Identities = 27/82 (32%), Positives = 47/82 (57%)
Frame = +2
Query: 278 VPTLHPTSEELSIGSMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKA 457
+PT+ E + ++ FT +D+GG + R +WR YF I+F+VD+ DR R+ E+
Sbjct: 46 IPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRERISEAHE 105
Query: 458 ELDSLLTDETLSNCPVLFSATR 523
EL +L ++ L + +L A +
Sbjct: 106 ELQRMLNEDELRDALLLVFANK 127
Score = 32.7 bits (71), Expect = 0.052
Identities = 13/29 (44%), Positives = 23/29 (79%)
Frame = +3
Query: 186 KKSGKLLFLGLDNAGKTTLLHMLKMIDLL 272
K+ ++L +GLD AGKTT+L+ LK+ +++
Sbjct: 15 KREMRILMVGLDAAGKTTILYKLKLGEIV 43
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 59.3 bits (137), Expect = 5e-10
Identities = 28/81 (34%), Positives = 45/81 (55%)
Frame = +2
Query: 281 PTLHPTSEELSIGSMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAE 460
PT L + +RFT +D+GG + R W++YF + +AI+++VD+ D RL E +
Sbjct: 46 PTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDLRLEECRNT 105
Query: 461 LDSLLTDETLSNCPVLFSATR 523
L LL +E L +L A +
Sbjct: 106 LQELLVEEKLLFTSILVLANK 126
Score = 31.1 bits (67), Expect = 0.16
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +3
Query: 180 LWKKSGKLLFLGLDNAGKTTLLHML 254
L ++ ++L LGLDNAGKTT+L L
Sbjct: 12 LKEREVRVLLLGLDNAGKTTILKCL 36
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 38.3 bits (85), Expect = 0.001
Identities = 32/129 (24%), Positives = 54/129 (41%), Gaps = 12/129 (9%)
Frame = +2
Query: 257 DDRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVD----- 421
DD L TL + ++ ++ FD+GG + RR W F V++I+F V
Sbjct: 170 DDILRSRNSTLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYD 229
Query: 422 -------ACDRPRLPESKAELDSLLTDETLSNCPVLFSATRLINPVLPVKTNYVTFSILY 580
A +R RL ES + DS++ + + ++ + + K +V F +
Sbjct: 230 KKLYERAAPERNRLVESISLFDSIINSQWFMHSSIILFLNKF--DLFRKKLEHVPFQDYF 287
Query: 581 QQTTGKGKV 607
Q GK V
Sbjct: 288 PQYEGKNSV 296
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 33.1 bits (72), Expect = 0.039
Identities = 17/55 (30%), Positives = 26/55 (47%)
Frame = +2
Query: 254 EDDRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLV 418
+ D L + T + E + + FD+GG + RR W F V A++FLV
Sbjct: 211 DQDILHCRIKTTGISEETFLLNRHHYRFFDVGGQRSERRKWIHCFENVTALLFLV 265
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 29.1 bits (62), Expect = 0.64
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = +1
Query: 52 ILQISAKTRDTLPLGLVINIVQFNYIQKCLSSTGSLECWDFLVYGKSRASSCFW 213
IL+ ++ TR+ L + ++ I++ L + GSL D L+ G S A FW
Sbjct: 633 ILRDASLTRNPLVYPSAKSSIKSIDIKRVLENVGSLNHEDILLIGSSNAKYSFW 686
>SPBC1271.12 |kes1||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 388
Score = 28.7 bits (61), Expect = 0.84
Identities = 11/38 (28%), Positives = 24/38 (63%)
Frame = +2
Query: 350 GHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAEL 463
G ++RRVW+++ A+DA + + ++ ++ E + EL
Sbjct: 306 GEYESRRVWKNFAVALDAGDYAAASQEKSKIEEGQREL 343
>SPCC191.11 |inv1||beta-fructofuranosidase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 581
Score = 28.3 bits (60), Expect = 1.1
Identities = 23/50 (46%), Positives = 30/50 (60%), Gaps = 7/50 (14%)
Frame = +2
Query: 467 SLLTDETLSNCPVLFSATRLI-NPV-LPVKTNY---VTFSILYQQ--TTG 595
SLL DETL PV+ S++ L +P+ LP T + VT SI Y + TTG
Sbjct: 411 SLLRDETLFTAPVINSSSSLSGSPITLPSNTAFEFNVTLSINYTEGCTTG 460
>SPAC10F6.11c |||kinase activator |Schizosaccharomyces pombe|chr
1|||Manual
Length = 481
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +2
Query: 485 TLSNCPVLFSATRLINPVLPVKTNYVTFSIL 577
T S C L S+T LI +L + NY TF+ L
Sbjct: 123 TASQCTRLDSSTALIKQLLDLVQNYPTFNQL 153
>SPCC11E10.04 |||mitochondrial ATPase expression protein
homolog|Schizosaccharomyces pombe|chr 3|||Manual
Length = 443
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +1
Query: 55 LQISAKTRDTLPLGLVINIVQFNYIQKCLSSTGSLECWDFLV 180
LQI +D LG+ NIV + +I + TG+ +++V
Sbjct: 113 LQIKLILQDADRLGIQFNIVDYTHILRVAMYTGNKHILEYIV 154
>SPBC1289.12 |usp109||U1 snRNP-associated protein
Usp109|Schizosaccharomyces pombe|chr 2|||Manual
Length = 352
Score = 26.2 bits (55), Expect = 4.5
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -1
Query: 244 KSVVFPALSSPRNKSLPDFFHRPRNPNTPVN 152
+S +PAL++P S+ D + P P +P+N
Sbjct: 256 QSTYWPALAAPVYPSMKDVPNNPFTPFSPIN 286
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 26.2 bits (55), Expect = 4.5
Identities = 18/66 (27%), Positives = 33/66 (50%)
Frame = +3
Query: 171 FLGLWKKSGKLLFLGLDNAGKTTLLHMLKMIDLLNMYLLYILHPRNCQ*AVCVSRRSTSV 350
FL ++ ++ ++F+G +GKTT + + D L H N Q A RR ++
Sbjct: 84 FLKIYHENQIIVFVGETGSGKTTQIPQFVLYDELP-------HLTNTQIACTQPRRVAAM 136
Query: 351 GISRRV 368
+++RV
Sbjct: 137 SVAKRV 142
>SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1206
Score = 25.8 bits (54), Expect = 5.9
Identities = 9/29 (31%), Positives = 20/29 (68%)
Frame = +1
Query: 43 QRNILQISAKTRDTLPLGLVINIVQFNYI 129
Q N++++ K DT+PL + +NI++ ++
Sbjct: 338 QGNVVELRLKYFDTVPLAVQLNILKTGFL 366
>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 2|||Manual
Length = 827
Score = 25.8 bits (54), Expect = 5.9
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -1
Query: 226 ALSSPRNKSLPDFFHRPRNPNTPVNQS 146
A S N LP F P + ++PVNQS
Sbjct: 73 AEESDPNLKLPSFLAPPNDKDSPVNQS 99
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 25.8 bits (54), Expect = 5.9
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = +3
Query: 141 ILDWFTGVLGFLGLWKKSGKLLFLGLDNAGKTTLLHMLKMIDLLNMYLLYI 293
+L F +LG+ WKK L L N + L L L++YL+++
Sbjct: 1040 LLFHFDSILGYQNYWKKVEPFLNLNSLNLLLKSELFQLDQCYSLSLYLIHL 1090
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/51 (23%), Positives = 27/51 (52%)
Frame = +1
Query: 439 SAGVQG*TRLVAH*RDAQ*LPCAVLGNKIDKPGAASEDELRHFFNSVSADH 591
SAGV+ T++V + +P + NK+D+ G++ +R + ++ +
Sbjct: 127 SAGVEAQTKVVWKQATKRGIPKVIFVNKMDRVGSSLGSTIRSIYTNLDCPY 177
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,780,221
Number of Sequences: 5004
Number of extensions: 55724
Number of successful extensions: 202
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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