BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0102 (691 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC776.02c |dis2|sds1, bws1|serine/threonine protein phosphatas... 219 2e-58 SPCC31H12.05c |sds21||serine/threonine protein phosphatase Sds21... 208 8e-55 SPAC57A7.08 |pzh1||serine/threonine protein phosphatase Pzh1|Sch... 181 1e-46 SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa... 129 4e-31 SPBC16H5.07c |ppa2||serine/threonine protein phosphatase Ppa2|Sc... 127 2e-30 SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 120 3e-28 SPBC26H8.05c |||serine/threonine protein phosphatase |Schizosacc... 119 5e-28 SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatas... 117 2e-27 SPAC22H10.04 |||protein phosphatase type 2A|Schizosaccharomyces ... 116 4e-27 SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosacch... 101 1e-22 SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 28 1.5 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 27 1.9 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 27 3.4 SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 26 4.5 SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2... 26 4.5 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 5.9 SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 26 5.9 SPAC1296.06 |||NADPH cytochrome reductase|Schizosaccharomyces po... 25 7.8 >SPBC776.02c |dis2|sds1, bws1|serine/threonine protein phosphatase PP1|Schizosaccharomyces pombe|chr 2|||Manual Length = 327 Score = 219 bits (536), Expect = 2e-58 Identities = 104/141 (73%), Positives = 116/141 (82%) Frame = +1 Query: 256 KSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS 435 K+REIF+SQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPE+NYLFLGDYVDRGKQS Sbjct: 40 KAREIFISQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPEANYLFLGDYVDRGKQS 99 Query: 436 LETICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCL 615 LE ICLLLAYKIKYPENFF+LRGN + NRIYGFYDECKR + + + FNCL Sbjct: 100 LEVICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRY-NIKLWKTFTDCFNCL 158 Query: 616 PVAAIVERGKSSGWSTGGLSP 678 P+AAI++ + GGLSP Sbjct: 159 PIAAIID--EKIFTMHGGLSP 177 Score = 42.7 bits (96), Expect = 5e-05 Identities = 17/34 (50%), Positives = 28/34 (82%) Frame = +2 Query: 152 ELNIDNVIKKLLKVRGEKPGMNVQLTEIEIKGLC 253 ++++D++I +LL+VRG +PG VQL+E EI+ LC Sbjct: 5 DVDLDSIIDRLLEVRGSRPGRQVQLSEDEIRFLC 38 >SPCC31H12.05c |sds21||serine/threonine protein phosphatase Sds21|Schizosaccharomyces pombe|chr 3|||Manual Length = 322 Score = 208 bits (507), Expect = 8e-55 Identities = 99/140 (70%), Positives = 111/140 (79%) Frame = +1 Query: 259 SREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSL 438 SR IFLSQP+LLELEAPLKICGDIHGQY DLLRLFEYGG+PP++NYLFLGDYVDRGKQSL Sbjct: 38 SRSIFLSQPMLLELEAPLKICGDIHGQYSDLLRLFEYGGYPPDANYLFLGDYVDRGKQSL 97 Query: 439 ETICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCLP 618 E ICLL AYKIKYPENFFLLRGN + NRIYGFYDECKR + + FNC+P Sbjct: 98 EVICLLFAYKIKYPENFFLLRGNHEFASINRIYGFYDECKRRY-SIKLWKTFTDCFNCMP 156 Query: 619 VAAIVERGKSSGWSTGGLSP 678 VAA+++ + GGLSP Sbjct: 157 VAAVID--EKIFCMHGGLSP 174 Score = 37.5 bits (83), Expect = 0.002 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 152 ELNIDNVIKKLLKVRGEKPGMNVQLTEIEIKGLC 253 + +ID +I+KL+K R KP VQL++ EI+ LC Sbjct: 2 DYDIDAIIEKLVKARNGKPSKQVQLSDAEIRYLC 35 >SPAC57A7.08 |pzh1||serine/threonine protein phosphatase Pzh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 515 Score = 181 bits (440), Expect = 1e-46 Identities = 91/139 (65%), Positives = 102/139 (73%) Frame = +1 Query: 262 REIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLE 441 REIFLSQP LLEL P+KI GD+HGQY DL+RLFE GFPP SNYLFLGDYVDRGKQSLE Sbjct: 227 REIFLSQPTLLELTPPVKIVGDVHGQYSDLIRLFEMCGFPPSSNYLFLGDYVDRGKQSLE 286 Query: 442 TICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCLPV 621 TI LL YKI+YPENFFLLRGN R+YGFYDECKR + + + FNCLP+ Sbjct: 287 TILLLFLYKIRYPENFFLLRGNHECANITRVYGFYDECKRRC-NIKIWKTFINTFNCLPI 345 Query: 622 AAIVERGKSSGWSTGGLSP 678 A++V GK GGLSP Sbjct: 346 ASVV-AGKIF-CVHGGLSP 362 >SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 129 bits (311), Expect = 4e-31 Identities = 59/140 (42%), Positives = 90/140 (64%) Frame = +1 Query: 259 SREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSL 438 ++E+ + + + P+ +CGDIHGQ++DL+ LF GG P++NYLF+GDYVDRG S+ Sbjct: 35 AKEVLSVESNVQSVRCPVTVCGDIHGQFHDLMELFNIGGPSPDTNYLFMGDYVDRGYHSV 94 Query: 439 ETICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCLP 618 ET+ LL+A+KI+YP+ +LRGN ++YGFYDEC R+ + + + F+ LP Sbjct: 95 ETVSLLIAFKIRYPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWQYFTDLFDYLP 154 Query: 619 VAAIVERGKSSGWSTGGLSP 678 + A++E GGLSP Sbjct: 155 LTALIE--DRIFCLHGGLSP 172 >SPBC16H5.07c |ppa2||serine/threonine protein phosphatase Ppa2|Schizosaccharomyces pombe|chr 2|||Manual Length = 322 Score = 127 bits (306), Expect = 2e-30 Identities = 59/141 (41%), Positives = 87/141 (61%) Frame = +1 Query: 256 KSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS 435 K+RE+ + + + P+ +CGDIHGQ++DL+ LF+ GG P+ NYLF+GDYVDRG S Sbjct: 47 KAREVLCQENNVQPVRNPVTVCGDIHGQFHDLMELFKIGGDVPDMNYLFMGDYVDRGYHS 106 Query: 436 LETICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCL 615 +ET+ LL+A K++YP +LRGN ++YGFYDEC R+ + + F+ Sbjct: 107 VETVSLLVAMKLRYPNRITILRGNHESRQITQVYGFYDECLRKYGSANVWKHFTNLFDYF 166 Query: 616 PVAAIVERGKSSGWSTGGLSP 678 P+ A++E GGLSP Sbjct: 167 PLTALIE--DRIFCLHGGLSP 185 >SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosaccharomyces pombe|chr 2|||Manual Length = 554 Score = 120 bits (288), Expect = 3e-28 Identities = 65/141 (46%), Positives = 85/141 (60%) Frame = +1 Query: 256 KSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS 435 K+ EI S+ L+E++AP+ +CGDIHGQYYDL++LFE GG P + YLFLGDYVDRG S Sbjct: 96 KATEILKSEDNLIEVDAPVTVCGDIHGQYYDLMKLFEVGGNPANTQYLFLGDYVDRGYFS 155 Query: 436 LETICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCL 615 +E + L A KI YP+ +LLRGN + F EC + + + FN L Sbjct: 156 IECLLYLWALKIWYPKTLWLLRGNHECAHLTDYFTFKLECTHKY-NIKVYEACLQSFNAL 214 Query: 616 PVAAIVERGKSSGWSTGGLSP 678 P+AAI+ K GGLSP Sbjct: 215 PLAAIM--NKQFLCVHGGLSP 233 >SPBC26H8.05c |||serine/threonine protein phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 348 Score = 119 bits (286), Expect = 5e-28 Identities = 63/139 (45%), Positives = 84/139 (60%) Frame = +1 Query: 262 REIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLE 441 R+I + + + + +P+ ICGDIHGQ +DLL LF GG P + YLFLGD+VDRG S+E Sbjct: 32 RDILIEESNIQWISSPVTICGDIHGQLHDLLELFRIGGSCPGTKYLFLGDFVDRGFYSVE 91 Query: 442 TICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCLPV 621 T LLL K KYP+ L+RGN ++YGFYDEC R+ + R F+ L + Sbjct: 92 TFLLLLTLKCKYPKEMTLIRGNHESRQITQVYGFYDECVRKYGSANVWRYCCEIFDYLSL 151 Query: 622 AAIVERGKSSGWSTGGLSP 678 A+V+ GK GGLSP Sbjct: 152 GALVD-GKVF-CVHGGLSP 168 >SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatase Ppe1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 305 Score = 117 bits (281), Expect = 2e-27 Identities = 56/137 (40%), Positives = 82/137 (59%) Frame = +1 Query: 268 IFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETI 447 I + + + + P+ +CGDIHGQ+YDLL LF GG P +NY+F+GD+VDRG SLET Sbjct: 32 ILMEESNIQPVRTPVTVCGDIHGQFYDLLELFRVGGELPSTNYIFMGDFVDRGYFSLETF 91 Query: 448 CLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCLPVAA 627 L + K +YP+ LLRGN ++YGFYDEC+ + + + + F+ L +AA Sbjct: 92 TLFMLLKARYPDKITLLRGNHESRQITQVYGFYDECQTKYGNANVWKYCCQVFDFLTLAA 151 Query: 628 IVERGKSSGWSTGGLSP 678 +++ GGLSP Sbjct: 152 VID--NKILCVHGGLSP 166 >SPAC22H10.04 |||protein phosphatase type 2A|Schizosaccharomyces pombe|chr 1|||Manual Length = 307 Score = 116 bits (278), Expect = 4e-27 Identities = 57/140 (40%), Positives = 85/140 (60%) Frame = +1 Query: 259 SREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSL 438 ++E+ + + ++ L P+ + GDIHGQ+ DLL +F GG P +NYLFLGDYVDRG S+ Sbjct: 29 AKEVLMQESNVVRLSTPITVVGDIHGQFDDLLEIFRIGGPCPYTNYLFLGDYVDRGYYSI 88 Query: 439 ETICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXFNCLP 618 ETI LL+ K++YP+ LLRGN + YGFY EC R+ + + + F+ L Sbjct: 89 ETITLLICLKLRYPKRITLLRGNHESRGITQTYGFYSECLRKYGNANVWKYFTDIFDFLT 148 Query: 619 VAAIVERGKSSGWSTGGLSP 678 ++A ++ + GGLSP Sbjct: 149 LSATID--DTIFCVHGGLSP 166 >SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 473 Score = 101 bits (241), Expect = 1e-22 Identities = 52/129 (40%), Positives = 76/129 (58%), Gaps = 5/129 (3%) Frame = +1 Query: 262 REIFLSQPILLEL----EAPLKICGDIHGQYYDLLRLFEYGGFP-PESNYLFLGDYVDRG 426 +E+ P L+++ + L ICGD HGQY+DLL +F+ G P P + YLF GD+VDRG Sbjct: 192 KELLEKTPSLIDIPVKGDETLVICGDTHGQYFDLLNIFKLHGPPSPTNKYLFNGDFVDRG 251 Query: 427 KQSLETICLLLAYKIKYPENFFLLRGNPSVPAFNRIYGFYDECKREV*H*TMGRRSQTXF 606 S E L AYK+ YP+ F+ RGN N++YGF EC+ + T S+T F Sbjct: 252 SWSTEVAFTLYAYKLLYPDAVFINRGNHETDDMNKVYGFEGECRSKYNERTFNIFSET-F 310 Query: 607 NCLPVAAIV 633 + LP+ +++ Sbjct: 311 SLLPLGSLI 319 >SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 27.9 bits (59), Expect = 1.5 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = -3 Query: 329 MSPQIFKGASSSSSIGCDKNISLDFNIA 246 +S Q+FK SS S + CD+ ++D +A Sbjct: 567 VSVQVFKDGSSDSEVRCDEYAAVDVMVA 594 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 27.5 bits (58), Expect = 1.9 Identities = 15/47 (31%), Positives = 26/47 (55%) Frame = -3 Query: 188 LVIFLLRYRCLTHLCLCLPSDILYVYEIWLNRIGKRREMFKIFADYE 48 ++ +LL Y+C + +P D+LY +E + IG R K++ YE Sbjct: 820 MLTYLLAYQCYFLIQPEMPLDMLYEFE---DVIGYVRPSMKVYMHYE 863 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 26.6 bits (56), Expect = 3.4 Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 6/75 (8%) Frame = +3 Query: 441 NNMPAPRLQD*ISREFLSFTRKPECASIQ*DLWVLR*VQKGGITL------NYGKTFTDX 602 +N+PAP + I S TR E + + + + + KG I+L NY K F+ Sbjct: 656 HNIPAPTNFNDIISFIPSITRLNELSQVSFNHFYF--LCKGDISLENILPYNYNKWFSFL 713 Query: 603 LQLSSRCGDS*TRKI 647 LQL C DS KI Sbjct: 714 LQLRKVCNDSEALKI 728 >SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1522 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = -2 Query: 477 IFNLVGEEQAYCFQRLLATVHIVSEE 400 ++NL G YCF RL T++ EE Sbjct: 1001 VYNLFGNMSLYCFFRLFHTLYSRLEE 1026 >SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 202 Score = 26.2 bits (55), Expect = 4.5 Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 6/73 (8%) Frame = +1 Query: 253 LKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRL------FEYGGFPPESNYLFLGDY 414 + ++ +FL+ P + +LE L+ G + L RL EY P + L + D Sbjct: 129 IDAQYVFLAPPSIEQLEVRLRGRGT-ENESAILQRLERARAEIEYSEKPGNFDALIVNDD 187 Query: 415 VDRGKQSLETICL 453 V++ + LE ICL Sbjct: 188 VEKAYKQLEAICL 200 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 25.8 bits (54), Expect = 5.9 Identities = 9/33 (27%), Positives = 17/33 (51%) Frame = +2 Query: 380 LRSPTISSSETMWTVASSRWKQYACSSPTRLNI 478 +R+ I S+ +W+ A W Y+ P R+ + Sbjct: 2097 VRACFIQGSQQVWSAALWYWGSYSTDKPNRMGV 2129 >SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +1 Query: 373 GFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKY 477 GF + + L LG Y RGKQS + L ++ Y Sbjct: 482 GFGEDLDCLILGGYFGRGKQSGKINSFLCGLRMDY 516 >SPAC1296.06 |||NADPH cytochrome reductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 558 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 1/37 (2%) Frame = +2 Query: 497 YAETRVCQHSIGFMGFTMSAKGRY-NIKLWEDVHRLA 604 + + + +HS G + T+ R NIK W+DV LA Sbjct: 163 FEDNNIVRHSRGKIEATLVHNKRISNIKHWQDVRHLA 199 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,983,380 Number of Sequences: 5004 Number of extensions: 64461 Number of successful extensions: 187 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 179 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 319939482 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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