Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0083
         (743 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|...    98   1e-21
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos...    38   0.002
SPAC1B1.03c |kap95||karyopherin Kap95|Schizosaccharomyces pombe|...    26   4.9  
SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi...    26   6.5  
SPBC3H7.01 |spo14|stl1, SPBP16F5.01c|WD repeat protein Spo14|Sch...    25   8.6  

>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1064

 Score = 98.3 bits (234), Expect = 1e-21
 Identities = 47/87 (54%), Positives = 63/87 (72%), Gaps = 4/87 (4%)
 Frame = +1

Query: 253 MYTVVPRLTKFIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPF 432
           +YTVVP+L   I+ +TNWY+R NR+RLKG++G  +   AL+ LF VLF +VR+M PFTPF
Sbjct: 712 LYTVVPQLLGLIEEMTNWYIRFNRRRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTPF 771

Query: 433 LTEFMYKTLRKLLPGD----SLDSVHF 501
           +TE +Y+ LR  +P D    SL SVHF
Sbjct: 772 ITENIYQHLRNYMPIDKNEISLRSVHF 798



 Score = 79.8 bits (188), Expect = 4e-16
 Identities = 38/85 (44%), Positives = 56/85 (65%), Gaps = 1/85 (1%)
 Frame = +2

Query: 2   SPVVKAENLRFKEEGVRDIVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKA-IREN 178
           SPVV+AE L+FKE+GVR++V  V +PW+N+++F         +    D+ F++ A +  N
Sbjct: 627 SPVVRAEILKFKEDGVREVVTRVLIPWWNSYKFFEAQAALYKKVTGKDFVFDDAATLSSN 686

Query: 179 VMDKWITSFTQSLIQFVKKEMAAYR 253
           VMD+WI +  QSLI FV +EM  YR
Sbjct: 687 VMDRWILARCQSLIGFVDEEMKQYR 711



 Score = 56.4 bits (130), Expect = 4e-09
 Identities = 27/64 (42%), Positives = 41/64 (64%)
 Frame = +3

Query: 510 PDPRIELVDTNIERAVQRMQSVIELGRVLRDRKTIPIKYPLPEMIVIHRDTXYLDDVKSL 689
           P  + EL D  + R V+RMQ++IEL R +R++  I +K PL  +IVI  +  YL+D K L
Sbjct: 802 PTYKSELDDETVLRRVKRMQTIIELARYVREQNNISLKTPLKTLIVILTNEEYLEDAKLL 861

Query: 690 GKYV 701
            +Y+
Sbjct: 862 ERYI 865


>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 980

 Score = 37.9 bits (84), Expect = 0.002
 Identities = 21/59 (35%), Positives = 33/59 (55%)
 Frame = +1

Query: 319 NRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPFLTEFMYKTLRKLLPGDSLDSV 495
           N K L  D      + A  TL+ VL + +R+M PF P++TE M++ L +  PGD   ++
Sbjct: 819 NSKYLLSDGTEVQQESAKQTLYTVLDNALRLMHPFMPYVTEEMWQRLPR-RPGDKTQTI 876


>SPAC1B1.03c |kap95||karyopherin Kap95|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 863

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 11/35 (31%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
 Frame = -1

Query: 476 PGSNFRKVLYINSVRKG-VNGAITLTMSNKTPNNV 375
           PG+NF  + Y++++R G V   + +T + +T N +
Sbjct: 740 PGANFSMIDYVDALRLGIVEAYVGITQAVRTDNRL 774


>SPAC20G8.04c |||mitochondrial electron transfer
           flavoprotein-ubiquinone
           oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 632

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 9/21 (42%), Positives = 12/21 (57%)
 Frame = -3

Query: 417 CHNSDHVKQDPKQCVESYLAI 355
           CH  DH  +D K C E+Y  +
Sbjct: 550 CHLFDHRPKDRKSCFETYKGV 570


>SPBC3H7.01 |spo14|stl1, SPBP16F5.01c|WD repeat protein
           Spo14|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 395

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 9/24 (37%), Positives = 15/24 (62%)
 Frame = +1

Query: 427 PFLTEFMYKTLRKLLPGDSLDSVH 498
           PF+   +Y  L+ L P + LD++H
Sbjct: 352 PFVLAILYFYLQLLFPDEKLDAIH 375


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,058,490
Number of Sequences: 5004
Number of extensions: 64730
Number of successful extensions: 184
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 353266144
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)