BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0083 (743 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative / i... 107 6e-24 At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)... 38 0.009 At1g73360.1 68414.m08491 homeobox-leucine zipper family protein ... 29 4.3 At5g16350.1 68418.m01911 expressed protein 28 7.5 At5g23720.1 68418.m02780 dual specificity protein phosphatase fa... 27 9.9 >At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative / isoleucine--tRNA ligase, putative similar to SP|P41252 Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5) (Isoleucine--tRNA ligase) (IleRS) (IRS) {Homo sapiens}; contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and V) Length = 1190 Score = 107 bits (258), Expect = 6e-24 Identities = 52/83 (62%), Positives = 60/83 (72%) Frame = +1 Query: 253 MYTVVPRLTKFIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPF 432 +YTVVPRL KF+D+LTN YVR NRKRLKG G DC AL TLF VL +VMAPFTPF Sbjct: 748 LYTVVPRLLKFLDNLTNIYVRFNRKRLKGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPF 807 Query: 433 LTEFMYKTLRKLLPGDSLDSVHF 501 TE +Y+ LRK G S +SVH+ Sbjct: 808 FTETLYQNLRKACKG-SEESVHY 829 Score = 103 bits (247), Expect = 1e-22 Identities = 50/86 (58%), Positives = 66/86 (76%), Gaps = 2/86 (2%) Frame = +2 Query: 2 SPVVKAENLRFKEEGVRDIVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRE-- 175 SPVV+AE LRFK+EGV +VKDVFLPWYNA+RFL+QN +RL E V + + A + Sbjct: 662 SPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDLATIQSA 721 Query: 176 NVMDKWITSFTQSLIQFVKKEMAAYR 253 N++D+WI S TQSL++FV++EM AYR Sbjct: 722 NILDQWIHSATQSLVRFVREEMDAYR 747 Score = 57.6 bits (133), Expect = 8e-09 Identities = 28/64 (43%), Positives = 41/64 (64%), Gaps = 1/64 (1%) Frame = +3 Query: 516 PRIELVDTNIERAVQRMQSVIELGRVLRDRKTIPIKYPLPEMIVIHRDTXYLDDVKS-LG 692 PR + IE +V RM +I+L R +R+R +P+K PL EMIV+H D +L+D+ L Sbjct: 834 PREGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLR 893 Query: 693 KYVL 704 +YVL Sbjct: 894 EYVL 897 >At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V) family protein similar to SP|P11931 Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (VALRS) {Bacillus stearothermophilus}; contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and V) Length = 970 Score = 37.5 bits (83), Expect = 0.009 Identities = 16/59 (27%), Positives = 31/59 (52%) Frame = +1 Query: 283 FIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPFLTEFMYKTL 459 F +WY+ ++ RL G G + L V ++++++ PF PF+TE +++ L Sbjct: 722 FWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTEDLWQAL 780 >At1g73360.1 68414.m08491 homeobox-leucine zipper family protein / lipid-binding START domain-containing protein protodermal factor2 (GI:14276060) [Arabidopsis thaliana]; similar to homeobox protein GI:1173621 from [ Phalaenopsis sp.] Length = 722 Score = 28.7 bits (61), Expect = 4.3 Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 4/40 (10%) Frame = -2 Query: 616 GIVFLSLRTLPNSITDCILWTAL--SILVS--TSSILGSG 509 GIVF++ L + DC+ WT L SI+ + T +++ SG Sbjct: 296 GIVFMNAMALVDMFMDCVKWTELFPSIIAASKTLAVISSG 335 >At5g16350.1 68418.m01911 expressed protein Length = 488 Score = 27.9 bits (59), Expect = 7.5 Identities = 18/70 (25%), Positives = 30/70 (42%) Frame = +2 Query: 56 IVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRENVMDKWITSFTQSLIQFVKK 235 +VKD W N F F++ + ++ D + Y KA+ + + T LI+ K Sbjct: 321 MVKDSKCRWGNYFSFIILPLSISLETDPLVYLNKSKAMMARTKHSYQAALTYFLIKISLK 380 Query: 236 EMAAYRCTQL 265 + A T L Sbjct: 381 VLGAKATTSL 390 >At5g23720.1 68418.m02780 dual specificity protein phosphatase family protein contains Pfam profile: PF00782 dual specificity phosphatase, catalytic domain; contains TG acceptor splice site at intron 9 Length = 929 Score = 27.5 bits (58), Expect = 9.9 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -1 Query: 644 YHFGQWIFYRNRFSISQNSSQFYNRLHSLD 555 Y F QW+ + S + SS F +RLH +D Sbjct: 54 YRFTQWLDLVRKRSATYGSSGFPHRLHRID 83 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,446,353 Number of Sequences: 28952 Number of extensions: 315056 Number of successful extensions: 774 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 760 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 773 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1643603136 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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