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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0083
         (743 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative / i...   107   6e-24
At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)...    38   0.009
At1g73360.1 68414.m08491 homeobox-leucine zipper family protein ...    29   4.3  
At5g16350.1 68418.m01911 expressed protein                             28   7.5  
At5g23720.1 68418.m02780 dual specificity protein phosphatase fa...    27   9.9  

>At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative /
           isoleucine--tRNA ligase, putative similar to SP|P41252
           Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5)
           (Isoleucine--tRNA ligase) (IleRS) (IRS) {Homo sapiens};
           contains Pfam profile PF00133: tRNA synthetases class I
           (I, L, M and V)
          Length = 1190

 Score =  107 bits (258), Expect = 6e-24
 Identities = 52/83 (62%), Positives = 60/83 (72%)
 Frame = +1

Query: 253 MYTVVPRLTKFIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPF 432
           +YTVVPRL KF+D+LTN YVR NRKRLKG  G  DC  AL TLF VL    +VMAPFTPF
Sbjct: 748 LYTVVPRLLKFLDNLTNIYVRFNRKRLKGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPF 807

Query: 433 LTEFMYKTLRKLLPGDSLDSVHF 501
            TE +Y+ LRK   G S +SVH+
Sbjct: 808 FTETLYQNLRKACKG-SEESVHY 829



 Score =  103 bits (247), Expect = 1e-22
 Identities = 50/86 (58%), Positives = 66/86 (76%), Gaps = 2/86 (2%)
 Frame = +2

Query: 2   SPVVKAENLRFKEEGVRDIVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRE-- 175
           SPVV+AE LRFK+EGV  +VKDVFLPWYNA+RFL+QN +RL  E  V +   + A  +  
Sbjct: 662 SPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDLATIQSA 721

Query: 176 NVMDKWITSFTQSLIQFVKKEMAAYR 253
           N++D+WI S TQSL++FV++EM AYR
Sbjct: 722 NILDQWIHSATQSLVRFVREEMDAYR 747



 Score = 57.6 bits (133), Expect = 8e-09
 Identities = 28/64 (43%), Positives = 41/64 (64%), Gaps = 1/64 (1%)
 Frame = +3

Query: 516  PRIELVDTNIERAVQRMQSVIELGRVLRDRKTIPIKYPLPEMIVIHRDTXYLDDVKS-LG 692
            PR  +    IE +V RM  +I+L R +R+R  +P+K PL EMIV+H D  +L+D+   L 
Sbjct: 834  PREGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLR 893

Query: 693  KYVL 704
            +YVL
Sbjct: 894  EYVL 897


>At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)
           family protein similar to SP|P11931 Valyl-tRNA
           synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (VALRS)
           {Bacillus stearothermophilus}; contains Pfam profile
           PF00133: tRNA synthetases class I (I, L, M and V)
          Length = 970

 Score = 37.5 bits (83), Expect = 0.009
 Identities = 16/59 (27%), Positives = 31/59 (52%)
 Frame = +1

Query: 283 FIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPFLTEFMYKTL 459
           F     +WY+  ++ RL G  G      +   L  V  ++++++ PF PF+TE +++ L
Sbjct: 722 FWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTEDLWQAL 780


>At1g73360.1 68414.m08491 homeobox-leucine zipper family protein /
           lipid-binding START domain-containing protein
           protodermal factor2 (GI:14276060) [Arabidopsis
           thaliana]; similar to homeobox protein GI:1173621 from [
           Phalaenopsis sp.]
          Length = 722

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 4/40 (10%)
 Frame = -2

Query: 616 GIVFLSLRTLPNSITDCILWTAL--SILVS--TSSILGSG 509
           GIVF++   L +   DC+ WT L  SI+ +  T +++ SG
Sbjct: 296 GIVFMNAMALVDMFMDCVKWTELFPSIIAASKTLAVISSG 335


>At5g16350.1 68418.m01911 expressed protein
          Length = 488

 Score = 27.9 bits (59), Expect = 7.5
 Identities = 18/70 (25%), Positives = 30/70 (42%)
 Frame = +2

Query: 56  IVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRENVMDKWITSFTQSLIQFVKK 235
           +VKD    W N F F++  +   ++ D + Y    KA+       +  + T  LI+   K
Sbjct: 321 MVKDSKCRWGNYFSFIILPLSISLETDPLVYLNKSKAMMARTKHSYQAALTYFLIKISLK 380

Query: 236 EMAAYRCTQL 265
            + A   T L
Sbjct: 381 VLGAKATTSL 390


>At5g23720.1 68418.m02780 dual specificity protein phosphatase
           family protein contains Pfam profile: PF00782 dual
           specificity phosphatase, catalytic domain; contains TG
           acceptor splice site at intron 9
          Length = 929

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -1

Query: 644 YHFGQWIFYRNRFSISQNSSQFYNRLHSLD 555
           Y F QW+    + S +  SS F +RLH +D
Sbjct: 54  YRFTQWLDLVRKRSATYGSSGFPHRLHRID 83


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,446,353
Number of Sequences: 28952
Number of extensions: 315056
Number of successful extensions: 774
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 760
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 773
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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