BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0083
(743 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative / i... 107 6e-24
At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)... 38 0.009
At1g73360.1 68414.m08491 homeobox-leucine zipper family protein ... 29 4.3
At5g16350.1 68418.m01911 expressed protein 28 7.5
At5g23720.1 68418.m02780 dual specificity protein phosphatase fa... 27 9.9
>At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative /
isoleucine--tRNA ligase, putative similar to SP|P41252
Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5)
(Isoleucine--tRNA ligase) (IleRS) (IRS) {Homo sapiens};
contains Pfam profile PF00133: tRNA synthetases class I
(I, L, M and V)
Length = 1190
Score = 107 bits (258), Expect = 6e-24
Identities = 52/83 (62%), Positives = 60/83 (72%)
Frame = +1
Query: 253 MYTVVPRLTKFIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPF 432
+YTVVPRL KF+D+LTN YVR NRKRLKG G DC AL TLF VL +VMAPFTPF
Sbjct: 748 LYTVVPRLLKFLDNLTNIYVRFNRKRLKGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPF 807
Query: 433 LTEFMYKTLRKLLPGDSLDSVHF 501
TE +Y+ LRK G S +SVH+
Sbjct: 808 FTETLYQNLRKACKG-SEESVHY 829
Score = 103 bits (247), Expect = 1e-22
Identities = 50/86 (58%), Positives = 66/86 (76%), Gaps = 2/86 (2%)
Frame = +2
Query: 2 SPVVKAENLRFKEEGVRDIVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRE-- 175
SPVV+AE LRFK+EGV +VKDVFLPWYNA+RFL+QN +RL E V + + A +
Sbjct: 662 SPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDLATIQSA 721
Query: 176 NVMDKWITSFTQSLIQFVKKEMAAYR 253
N++D+WI S TQSL++FV++EM AYR
Sbjct: 722 NILDQWIHSATQSLVRFVREEMDAYR 747
Score = 57.6 bits (133), Expect = 8e-09
Identities = 28/64 (43%), Positives = 41/64 (64%), Gaps = 1/64 (1%)
Frame = +3
Query: 516 PRIELVDTNIERAVQRMQSVIELGRVLRDRKTIPIKYPLPEMIVIHRDTXYLDDVKS-LG 692
PR + IE +V RM +I+L R +R+R +P+K PL EMIV+H D +L+D+ L
Sbjct: 834 PREGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLR 893
Query: 693 KYVL 704
+YVL
Sbjct: 894 EYVL 897
>At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)
family protein similar to SP|P11931 Valyl-tRNA
synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (VALRS)
{Bacillus stearothermophilus}; contains Pfam profile
PF00133: tRNA synthetases class I (I, L, M and V)
Length = 970
Score = 37.5 bits (83), Expect = 0.009
Identities = 16/59 (27%), Positives = 31/59 (52%)
Frame = +1
Query: 283 FIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPFLTEFMYKTL 459
F +WY+ ++ RL G G + L V ++++++ PF PF+TE +++ L
Sbjct: 722 FWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTEDLWQAL 780
>At1g73360.1 68414.m08491 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein
protodermal factor2 (GI:14276060) [Arabidopsis
thaliana]; similar to homeobox protein GI:1173621 from [
Phalaenopsis sp.]
Length = 722
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 4/40 (10%)
Frame = -2
Query: 616 GIVFLSLRTLPNSITDCILWTAL--SILVS--TSSILGSG 509
GIVF++ L + DC+ WT L SI+ + T +++ SG
Sbjct: 296 GIVFMNAMALVDMFMDCVKWTELFPSIIAASKTLAVISSG 335
>At5g16350.1 68418.m01911 expressed protein
Length = 488
Score = 27.9 bits (59), Expect = 7.5
Identities = 18/70 (25%), Positives = 30/70 (42%)
Frame = +2
Query: 56 IVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRENVMDKWITSFTQSLIQFVKK 235
+VKD W N F F++ + ++ D + Y KA+ + + T LI+ K
Sbjct: 321 MVKDSKCRWGNYFSFIILPLSISLETDPLVYLNKSKAMMARTKHSYQAALTYFLIKISLK 380
Query: 236 EMAAYRCTQL 265
+ A T L
Sbjct: 381 VLGAKATTSL 390
>At5g23720.1 68418.m02780 dual specificity protein phosphatase
family protein contains Pfam profile: PF00782 dual
specificity phosphatase, catalytic domain; contains TG
acceptor splice site at intron 9
Length = 929
Score = 27.5 bits (58), Expect = 9.9
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 644 YHFGQWIFYRNRFSISQNSSQFYNRLHSLD 555
Y F QW+ + S + SS F +RLH +D
Sbjct: 54 YRFTQWLDLVRKRSATYGSSGFPHRLHRID 83
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,446,353
Number of Sequences: 28952
Number of extensions: 315056
Number of successful extensions: 774
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 760
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 773
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -