BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0075 (702 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g35020.1 68415.m04296 UTP--glucose-1-phosphate uridylyltransf... 76 2e-14 At1g31070.2 68414.m03804 UDP-N-acetylglucosamine pyrophosphoryla... 72 3e-13 At1g31070.1 68414.m03803 UDP-N-acetylglucosamine pyrophosphoryla... 47 1e-05 At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 30 1.7 At1g10770.1 68414.m01233 invertase/pectin methylesterase inhibit... 27 9.1 >At2g35020.1 68415.m04296 UTP--glucose-1-phosphate uridylyltransferase family protein similar to SP|Q16222 UDP-N-acetylhexosamine pyrophosphorylase (Antigen X) {Homo sapiens}; contains Pfam profile PF01704: UTP--glucose-1-phosphate uridylyltransferase Length = 502 Score = 76.2 bits (179), Expect = 2e-14 Identities = 45/120 (37%), Positives = 62/120 (51%), Gaps = 5/120 (4%) Frame = +3 Query: 357 SVPNLTPDKIEEYENIGFKEICNGKVGVLLLAGGQATRLGFGHPKGMYDVGCHQEKPCSR 536 +V T + E++ +G K I GK+GV+LL+GGQ TRLG PKG Y++G K + Sbjct: 100 TVEERTKEDREKWWKMGLKAIYEGKLGVVLLSGGQGTRLGSSDPKGCYNIGLPSGKSLFQ 159 Query: 537 YKPRGSWRVQQMAAEKYGNEG-----KITWYIMTSETHESPYCKIISKSHSYFGLNENDV 701 + VQ++A++ I WYIMTS P K KSH YFGL + V Sbjct: 160 IQAERILCVQRLASQAMSEASPTRPVTIQWYIMTSPFTHEPTQKFF-KSHKYFGLEPDQV 218 Score = 37.9 bits (84), Expect = 0.006 Identities = 19/52 (36%), Positives = 29/52 (55%) Frame = +1 Query: 136 YETLLRNLKDHGQEHLIKYWSVLSEEQRKQLSDEILKLDLRKCMQHFRGQLN 291 ++ L+ LKD+GQE + W LS E+R L +I LDL + + R L+ Sbjct: 30 HQALVERLKDYGQEDVFSLWDELSPEERDLLLRDIENLDLPRIDRIIRCSLH 81 >At1g31070.2 68414.m03804 UDP-N-acetylglucosamine pyrophosphorylase-related low similarity to SP|P43123 UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) {Saccharomyces cerevisiae} Length = 505 Score = 72.1 bits (169), Expect = 3e-13 Identities = 45/120 (37%), Positives = 65/120 (54%), Gaps = 5/120 (4%) Frame = +3 Query: 357 SVPNLTPDKIEEYENIGFKEICNGKVGVLLLAGGQATRLGFGHPKGMYDVGCHQEKPCSR 536 +V T + E++ +G K I GK+GV+LL+GGQ TRLG PKG +++G K + Sbjct: 104 TVDGRTMEDREKWWKMGLKTIYEGKLGVVLLSGGQGTRLGSSDPKGCFNIGLPSGKSLFQ 163 Query: 537 YKPRGSWRVQQMAAEKYGNEGKIT-----WYIMTSETHESPYCKIISKSHSYFGLNENDV 701 + VQ++AA+ +EG I WYIMTS + K S SH YFGL + + Sbjct: 164 IQAERILCVQRLAAQVV-SEGPIRPVTIHWYIMTSPFTDEATRKYFS-SHKYFGLEPDQI 221 Score = 34.7 bits (76), Expect = 0.060 Identities = 17/51 (33%), Positives = 28/51 (54%) Frame = +1 Query: 139 ETLLRNLKDHGQEHLIKYWSVLSEEQRKQLSDEILKLDLRKCMQHFRGQLN 291 + L+ LKD+GQE + W LS +++ L +I LDL + + R L+ Sbjct: 35 QALVERLKDYGQEDIFSLWDELSPDEKDFLVRDIENLDLPRIDRIIRCSLH 85 >At1g31070.1 68414.m03803 UDP-N-acetylglucosamine pyrophosphorylase-related low similarity to SP|P43123 UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) {Saccharomyces cerevisiae} Length = 153 Score = 47.2 bits (107), Expect = 1e-05 Identities = 22/48 (45%), Positives = 31/48 (64%) Frame = +3 Query: 357 SVPNLTPDKIEEYENIGFKEICNGKVGVLLLAGGQATRLGFGHPKGMY 500 +V T + E++ +G K I GK+GV+LL+GGQ TRLG PKG + Sbjct: 104 TVDGRTMEDREKWWKMGLKTIYEGKLGVVLLSGGQGTRLGSSDPKGCF 151 Score = 34.7 bits (76), Expect = 0.060 Identities = 17/51 (33%), Positives = 28/51 (54%) Frame = +1 Query: 139 ETLLRNLKDHGQEHLIKYWSVLSEEQRKQLSDEILKLDLRKCMQHFRGQLN 291 + L+ LKD+GQE + W LS +++ L +I LDL + + R L+ Sbjct: 35 QALVERLKDYGQEDIFSLWDELSPDEKDFLVRDIENLDLPRIDRIIRCSLH 85 >At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) identical to GB:X95295. Based on identical cDNA hits, the translation is now 40 AAs longer at the N-terminal, and start of exon2 is also corrected. Length = 263 Score = 29.9 bits (64), Expect = 1.7 Identities = 17/66 (25%), Positives = 30/66 (45%) Frame = +1 Query: 61 HAF*SSYTVDEHLNLFVNKSRSEKMYETLLRNLKDHGQEHLIKYWSVLSEEQRKQLSDEI 240 H F S + + + + NKS S ++ ++K HG VL+ K L E+ Sbjct: 20 HTFLHSSSSNSNFKIRSNKSSSSSSSSIIMASIKVHGVPMSTATMRVLATLYEKDLQFEL 79 Query: 241 LKLDLR 258 + +D+R Sbjct: 80 IPVDMR 85 >At1g10770.1 68414.m01233 invertase/pectin methylesterase inhibitor family protein contains Pfam profile PF04043: Plant invertase/pectin methylesterase inhibitor Length = 167 Score = 27.5 bits (58), Expect = 9.1 Identities = 21/82 (25%), Positives = 39/82 (47%) Frame = +3 Query: 42 AGQWASSRFLIVVYGRRAFKFVCKQIAE*KNV*NIITKPKRSWSRTLN*ILVGIK*GTAE 221 AGQ A SR +I + A+ +C+ + + +T P+++ RT+ + K AE Sbjct: 26 AGQVAESR-MINICSHTAYPSLCRPLVK------RVTSPRKATHRTIQALEAKTKLALAE 78 Query: 222 TAVGRNSKT*LTEVHATFSRAI 287 TA +N ++ + T A+ Sbjct: 79 TARFKNGNQAVSTCYETLGDAV 100 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,517,191 Number of Sequences: 28952 Number of extensions: 331197 Number of successful extensions: 851 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 809 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 846 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1506636208 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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