SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0064
         (703 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical...    58   6e-09
At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ...    58   7e-09
At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ...    48   8e-06
At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ...    35   0.045
At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-...    34   0.079
At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ...    32   0.42 
At2g34960.1 68415.m04290 amino acid permease family protein simi...    27   9.1  

>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
           Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
           thaliana]; identical to cDNA SMT3 protein GI:1707371
          Length = 100

 Score = 58.0 bits (134), Expect = 6e-09
 Identities = 28/51 (54%), Positives = 35/51 (68%), Gaps = 1/51 (1%)
 Frame = +2

Query: 104 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVV 253
           D+K G+   HINLKV GQD   V F+IK+ T L+KLMNAYCDR  + M  +
Sbjct: 8   DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNSI 58



 Score = 41.1 bits (92), Expect = 7e-04
 Identities = 19/43 (44%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
 Frame = +1

Query: 232 RSINAGS-AFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 357
           +S++  S AF FDG+ +    TP  L+ME+GD I+    QTGG
Sbjct: 51  QSVDMNSIAFLFDGRRLRAEQTPDELDMEDGDEIDAMLHQTGG 93


>At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852 from
           [Arabidopsis thaliana]; identical to cDNA small
           ubiquitin-like modifier 2 (SUMO)  GI:22652843; contains
           Pfam profile PF00240: Ubiquitin family
          Length = 103

 Score = 57.6 bits (133), Expect = 7e-09
 Identities = 26/50 (52%), Positives = 33/50 (66%)
 Frame = +2

Query: 104 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVV 253
           D+K  +  HINLKV GQD   V F+IK+ T L+KLMNAYCDR  +    +
Sbjct: 8   DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDFNSI 57



 Score = 41.9 bits (94), Expect = 4e-04
 Identities = 18/35 (51%), Positives = 22/35 (62%)
 Frame = +1

Query: 253 AFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 357
           AF FDG+ +    TP  LEME+GD I+    QTGG
Sbjct: 58  AFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGG 92


>At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO)
           similar to SP|O13351 Ubiquitin-like protein smt3/pmt3
           {Schizosaccharomyces pombe}; identical to cDNA small
           ubiquitin-like modifier 3 (SUMO) GI:22652845
          Length = 111

 Score = 47.6 bits (108), Expect = 8e-06
 Identities = 22/42 (52%), Positives = 26/42 (61%)
 Frame = +2

Query: 119 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSM 244
           +  H+ LKV  QD   V FK KK  PL+KLM  YCDR GL +
Sbjct: 14  QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKL 55



 Score = 35.1 bits (77), Expect = 0.045
 Identities = 15/36 (41%), Positives = 23/36 (63%)
 Frame = +1

Query: 253 AFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGGV 360
           AF F+G  I   +TP  L+ME+GD I+  +  +GG+
Sbjct: 59  AFIFNGARIGGLETPDELDMEDGDVIDACRAMSGGL 94


>At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852
           [Arabidopsis thaliana]; contains INTERPRO:IPR000626
           ubiquitin domain; contains Pfam profile PF00240:
           Ubiquitin family; contains Pfam profile PF00240:
           Ubiquitin family;  identical to cDNA small
           ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847
          Length = 108

 Score = 35.1 bits (77), Expect = 0.045
 Identities = 18/56 (32%), Positives = 27/56 (48%)
 Frame = +2

Query: 86  INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVV 253
           ++ K    +   +  + LKV  Q  A   +KI  H  L+KLM+AYC +  L    V
Sbjct: 13  VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNLDYSSV 68



 Score = 28.3 bits (60), Expect = 5.2
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +1

Query: 256 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 357
           F ++G+ I    TP  L MEE D I +  +  GG
Sbjct: 70  FVYNGREIKARQTPAQLHMEEEDEICMVMELGGG 103


>At1g68185.1 68414.m07789 ubiquitin-related similar to
           ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission
           yeast]
          Length = 215

 Score = 34.3 bits (75), Expect = 0.079
 Identities = 15/32 (46%), Positives = 22/32 (68%)
 Frame = +1

Query: 256 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQT 351
           F FDG  I+ + TP+ L ME+ D IEV+ ++T
Sbjct: 184 FIFDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215


>At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351
           Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces
           pombe}
          Length = 114

 Score = 31.9 bits (69), Expect = 0.42
 Identities = 15/34 (44%), Positives = 18/34 (52%)
 Frame = +1

Query: 256 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 357
           F FDG  I E  TP  LE ++GD I+    Q  G
Sbjct: 71  FLFDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104


>At2g34960.1 68415.m04290 amino acid permease family protein similar
           to cationic amino acid transporter 3 [Rattus norvegicus]
           GI:2116552; contains Pfam profile PF00324: Amino acid
           permease
          Length = 569

 Score = 27.5 bits (58), Expect = 9.1
 Identities = 15/37 (40%), Positives = 18/37 (48%)
 Frame = -2

Query: 297 WSVILIYWLPIKSECTTCIDRPALSQ*AFINFLRGVC 187
           W V L+ WLP  S  T      +L   AF+ F  GVC
Sbjct: 507 WGVPLVPWLPCLSIATNIFLMGSLGAMAFVRF--GVC 541


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,490,851
Number of Sequences: 28952
Number of extensions: 261927
Number of successful extensions: 553
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 544
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 553
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -