Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0048
(805 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q0IEN7 Cluster: Glutathione synthetase; n=7; Endopteryg... 68 2e-10
UniRef50_A3FP33 Cluster: Glutathione synthetase; n=11; Diptera|R... 64 3e-09
UniRef50_UPI00015B422A Cluster: PREDICTED: similar to glutathion... 49 1e-04
UniRef50_A6XGV2 Cluster: Glutathione synthetase; n=1; Polytomell... 48 2e-04
UniRef50_P46416 Cluster: Glutathione synthetase, chloroplast pre... 48 3e-04
UniRef50_UPI0000587A6B Cluster: PREDICTED: hypothetical protein;... 47 6e-04
UniRef50_Q017J0 Cluster: Glutathione synthetase; n=2; Ostreococc... 46 0.001
UniRef50_Q2QPI2 Cluster: Glutathione synthetase, chloroplast, pu... 45 0.002
UniRef50_Q5DCB1 Cluster: SJCHGC05152 protein; n=1; Schistosoma j... 45 0.002
UniRef50_P48637 Cluster: Glutathione synthetase; n=31; Euteleost... 45 0.003
UniRef50_Q54E83 Cluster: Glutathione synthase; n=1; Dictyosteliu... 44 0.006
UniRef50_Q8X1E5 Cluster: Glutathione synthetase; n=3; Saccharomy... 43 0.008
UniRef50_Q5KAQ7 Cluster: Glutathione synthase, putative; n=1; Fi... 42 0.024
UniRef50_A3LSH4 Cluster: Glutathione synthetase; n=2; Saccharomy... 41 0.032
UniRef50_Q4P309 Cluster: Putative uncharacterized protein; n=1; ... 41 0.042
UniRef50_Q4T6E7 Cluster: Chromosome undetermined SCAF8803, whole... 40 0.073
UniRef50_A4R8J8 Cluster: Putative uncharacterized protein; n=5; ... 39 0.13
UniRef50_A2Y1U6 Cluster: Putative uncharacterized protein; n=2; ... 38 0.22
UniRef50_Q4QFP4 Cluster: Glutathione synthetase, putative; n=3; ... 38 0.30
UniRef50_A5K9N5 Cluster: Glutathione synthetase, putative; n=6; ... 37 0.52
UniRef50_Q08220 Cluster: Glutathione synthetase; n=9; Saccharomy... 37 0.52
UniRef50_UPI0000E4866A Cluster: PREDICTED: similar to glutathion... 37 0.68
UniRef50_A1CZA9 Cluster: Glutathione synthetase, putative; n=9; ... 36 0.90
UniRef50_Q57UN0 Cluster: Glutathione synthetase, putative; n=3; ... 36 1.2
UniRef50_Q080F9 Cluster: Glutathione synthase; n=7; Gammaproteob... 36 1.6
UniRef50_Q21549 Cluster: Putative uncharacterized protein; n=2; ... 36 1.6
UniRef50_Q9HEY0 Cluster: Glutathione synthetase Gsh1; n=1; Pneum... 34 4.8
UniRef50_Q8MTQ0 Cluster: Putative uncharacterized protein; n=1; ... 33 6.4
>UniRef50_Q0IEN7 Cluster: Glutathione synthetase; n=7;
Endopterygota|Rep: Glutathione synthetase - Aedes
aegypti (Yellowfever mosquito)
Length = 495
Score = 68.1 bits (159), Expect = 2e-10
Identities = 30/34 (88%), Positives = 33/34 (97%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLLD 106
N+QVGHMLRTKL+ ANEGGVAAGLGALDSPYL+D
Sbjct: 462 NKQVGHMLRTKLSSANEGGVAAGLGALDSPYLID 495
>UniRef50_A3FP33 Cluster: Glutathione synthetase; n=11; Diptera|Rep:
Glutathione synthetase - Drosophila melanogaster (Fruit
fly)
Length = 562
Score = 64.5 bits (150), Expect = 3e-09
Identities = 29/34 (85%), Positives = 31/34 (91%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLLD 106
N Q GHMLRTKL+ ANEGGVAAGLGALDSPYL+D
Sbjct: 521 NYQAGHMLRTKLSTANEGGVAAGLGALDSPYLID 554
>UniRef50_UPI00015B422A Cluster: PREDICTED: similar to glutathione
synthetase; n=1; Nasonia vitripennis|Rep: PREDICTED:
similar to glutathione synthetase - Nasonia vitripennis
Length = 483
Score = 49.2 bits (112), Expect = 1e-04
Identities = 22/33 (66%), Positives = 26/33 (78%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLL 103
N VGH LRTKLA +NEGGV++G GA DSP L+
Sbjct: 444 NLAVGHTLRTKLAKSNEGGVSSGFGACDSPLLV 476
>UniRef50_A6XGV2 Cluster: Glutathione synthetase; n=1; Polytomella
parva|Rep: Glutathione synthetase - Polytomella parva
Length = 105
Score = 48.4 bits (110), Expect = 2e-04
Identities = 20/32 (62%), Positives = 25/32 (78%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYL 100
N GH++RTK A++NEGGVAAG L+SPYL
Sbjct: 74 NAMAGHLVRTKAANSNEGGVAAGFAVLNSPYL 105
>UniRef50_P46416 Cluster: Glutathione synthetase, chloroplast
precursor; n=41; Magnoliophyta|Rep: Glutathione
synthetase, chloroplast precursor - Arabidopsis thaliana
(Mouse-ear cress)
Length = 539
Score = 48.0 bits (109), Expect = 3e-04
Identities = 20/33 (60%), Positives = 27/33 (81%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLL 103
N Q G+++RTK+A ++EGGVAAG G LDS YL+
Sbjct: 507 NEQSGYLMRTKIASSDEGGVAAGFGVLDSIYLI 539
>UniRef50_UPI0000587A6B Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 429
Score = 46.8 bits (106), Expect = 6e-04
Identities = 20/32 (62%), Positives = 23/32 (71%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYL 100
N GH+LRTK D NEGGVAAG +DSP+L
Sbjct: 397 NESEGHLLRTKHTDENEGGVAAGASCVDSPFL 428
>UniRef50_Q017J0 Cluster: Glutathione synthetase; n=2;
Ostreococcus|Rep: Glutathione synthetase - Ostreococcus
tauri
Length = 469
Score = 46.0 bits (104), Expect = 0.001
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLL 103
N+ GH+LRTK A ++EGGVAAG LDSP L+
Sbjct: 437 NKHGGHLLRTKAASSDEGGVAAGYAVLDSPLLV 469
>UniRef50_Q2QPI2 Cluster: Glutathione synthetase, chloroplast,
putative, expressed; n=4; Oryza sativa|Rep: Glutathione
synthetase, chloroplast, putative, expressed - Oryza
sativa subsp. japonica (Rice)
Length = 488
Score = 45.2 bits (102), Expect = 0.002
Identities = 19/34 (55%), Positives = 26/34 (76%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLLD 106
N Q G+++RTK++ ++EGGVAAG LDS YL D
Sbjct: 452 NEQSGYLMRTKVSSSDEGGVAAGFAVLDSLYLTD 485
>UniRef50_Q5DCB1 Cluster: SJCHGC05152 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC05152 protein - Schistosoma
japonicum (Blood fluke)
Length = 139
Score = 45.2 bits (102), Expect = 0.002
Identities = 18/33 (54%), Positives = 25/33 (75%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLL 103
N GH+LR+K ++NEGG+ AG G LDSP+L+
Sbjct: 107 NECSGHLLRSKPLESNEGGIVAGFGCLDSPFLV 139
>UniRef50_P48637 Cluster: Glutathione synthetase; n=31;
Euteleostomi|Rep: Glutathione synthetase - Homo sapiens
(Human)
Length = 474
Score = 44.8 bits (101), Expect = 0.003
Identities = 18/31 (58%), Positives = 24/31 (77%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPY 97
N+ VGH+LRTK + +GGVAAG+ LD+PY
Sbjct: 442 NKHVGHLLRTKAIEHADGGVAAGVAVLDNPY 472
>UniRef50_Q54E83 Cluster: Glutathione synthase; n=1; Dictyostelium
discoideum AX4|Rep: Glutathione synthase - Dictyostelium
discoideum AX4
Length = 476
Score = 43.6 bits (98), Expect = 0.006
Identities = 20/32 (62%), Positives = 25/32 (78%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYL 100
N+Q G +LRTK A+++E GVAAG G LDSP L
Sbjct: 444 NKQAGILLRTKTANSDEVGVAAGFGLLDSPIL 475
>UniRef50_Q8X1E5 Cluster: Glutathione synthetase; n=3;
Saccharomycetales|Rep: Glutathione synthetase - Pichia
angusta (Yeast) (Hansenula polymorpha)
Length = 473
Score = 43.2 bits (97), Expect = 0.008
Identities = 19/34 (55%), Positives = 25/34 (73%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLLD 106
N G++LR+K + +NEGGVAAG G +DS YL D
Sbjct: 440 NDYAGYLLRSKTSTSNEGGVAAGFGCVDSLYLHD 473
>UniRef50_Q5KAQ7 Cluster: Glutathione synthase, putative; n=1;
Filobasidiella neoformans|Rep: Glutathione synthase,
putative - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 516
Score = 41.5 bits (93), Expect = 0.024
Identities = 17/34 (50%), Positives = 26/34 (76%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLLD 106
N + G +LRTK +++EGGVA G+ ++DSP L+D
Sbjct: 483 NERAGTLLRTKGRESDEGGVAIGISSIDSPLLVD 516
>UniRef50_A3LSH4 Cluster: Glutathione synthetase; n=2;
Saccharomycetaceae|Rep: Glutathione synthetase - Pichia
stipitis (Yeast)
Length = 491
Score = 41.1 bits (92), Expect = 0.032
Identities = 17/32 (53%), Positives = 24/32 (75%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYL 100
N+ G +LR+K + +NEGGVAAG G +D+ YL
Sbjct: 459 NKTSGWLLRSKFSSSNEGGVAAGFGCVDNVYL 490
>UniRef50_Q4P309 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 542
Score = 40.7 bits (91), Expect = 0.042
Identities = 16/29 (55%), Positives = 23/29 (79%)
Frame = +2
Query: 17 GHMLRTKLADANEGGVAAGLGALDSPYLL 103
G++LRTK D++EGGVA G +DSP+L+
Sbjct: 514 GYLLRTKGRDSDEGGVAVGFSVIDSPFLI 542
>UniRef50_Q4T6E7 Cluster: Chromosome undetermined SCAF8803, whole
genome shotgun sequence; n=3; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF8803,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 525
Score = 39.9 bits (89), Expect = 0.073
Identities = 17/30 (56%), Positives = 22/30 (73%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSP 94
N VGH+LRTK ++ +GGVAAG+ LD P
Sbjct: 495 NECVGHLLRTKSSEHADGGVAAGVAVLDDP 524
>UniRef50_A4R8J8 Cluster: Putative uncharacterized protein; n=5;
Pezizomycotina|Rep: Putative uncharacterized protein -
Magnaporthe grisea (Rice blast fungus) (Pyricularia
grisea)
Length = 523
Score = 39.1 bits (87), Expect = 0.13
Identities = 18/33 (54%), Positives = 23/33 (69%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLL 103
N Q G++LRTK + EGGVAAG G +DS L+
Sbjct: 491 NEQEGYLLRTKGDQSEEGGVAAGFGCMDSVALV 523
>UniRef50_A2Y1U6 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 548
Score = 38.3 bits (85), Expect = 0.22
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYL 100
N Q G+++RTK A NEGGV AG L+S +L
Sbjct: 516 NDQCGYLMRTKAASLNEGGVVAGYAFLNSVFL 547
>UniRef50_Q4QFP4 Cluster: Glutathione synthetase, putative; n=3;
Leishmania|Rep: Glutathione synthetase, putative -
Leishmania major
Length = 606
Score = 37.9 bits (84), Expect = 0.30
Identities = 17/33 (51%), Positives = 24/33 (72%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLL 103
N+ G ++RTK ADA +GGV AG+ ALDS ++
Sbjct: 573 NQAAGTLVRTKPADAADGGVIAGVAALDSVQII 605
>UniRef50_A5K9N5 Cluster: Glutathione synthetase, putative; n=6;
Plasmodium|Rep: Glutathione synthetase, putative -
Plasmodium vivax
Length = 659
Score = 37.1 bits (82), Expect = 0.52
Identities = 16/33 (48%), Positives = 23/33 (69%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLL 103
N Q G+++RTK A NEGG G+ +LDS +L+
Sbjct: 627 NEQKGYLVRTKNARENEGGAICGISSLDSFFLV 659
>UniRef50_Q08220 Cluster: Glutathione synthetase; n=9;
Saccharomycetales|Rep: Glutathione synthetase -
Saccharomyces cerevisiae (Baker's yeast)
Length = 491
Score = 37.1 bits (82), Expect = 0.52
Identities = 17/29 (58%), Positives = 20/29 (68%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDS 91
N G +LR+K +NEGGVAAG G LDS
Sbjct: 459 NEFSGSLLRSKFNTSNEGGVAAGFGCLDS 487
>UniRef50_UPI0000E4866A Cluster: PREDICTED: similar to glutathione
synthetase type A1; n=1; Strongylocentrotus
purpuratus|Rep: PREDICTED: similar to glutathione
synthetase type A1 - Strongylocentrotus purpuratus
Length = 222
Score = 36.7 bits (81), Expect = 0.68
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYL 100
N+ G++ RTK +EGG+ G G LD+PYL
Sbjct: 175 NKTTGYLYRTKDIQLDEGGLMVGTGFLDTPYL 206
>UniRef50_A1CZA9 Cluster: Glutathione synthetase, putative; n=9;
Pezizomycotina|Rep: Glutathione synthetase, putative -
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL
181)(Aspergillus fischerianus (strain ATCC 1020 / DSM
3700 / NRRL 181))
Length = 516
Score = 36.3 bits (80), Expect = 0.90
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDS 91
N Q G ++RTK NEGGVA G +LDS
Sbjct: 484 NEQGGWLMRTKAKGVNEGGVATGFSSLDS 512
>UniRef50_Q57UN0 Cluster: Glutathione synthetase, putative; n=3;
Trypanosoma|Rep: Glutathione synthetase, putative -
Trypanosoma brucei
Length = 555
Score = 35.9 bits (79), Expect = 1.2
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = +2
Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDS 91
N G+ +R+K ADA++GGV AG+ ALDS
Sbjct: 522 NTFAGYTVRSKPADADDGGVMAGVAALDS 550
>UniRef50_Q080F9 Cluster: Glutathione synthase; n=7;
Gammaproteobacteria|Rep: Glutathione synthase -
Shewanella frigidimarina (strain NCIMB 400)
Length = 495
Score = 35.5 bits (78), Expect = 1.6
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = +2
Query: 17 GHMLRTKLADANEGGVAAGLGALDSPYLLD 106
G+++R+K A NEGG+ +G G LDS L+D
Sbjct: 466 GYLIRSKPASENEGGIHSGKGILDSLTLID 495
>UniRef50_Q21549 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 490
Score = 35.5 bits (78), Expect = 1.6
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +2
Query: 8 RQVGHMLRTKLADANEGGVAAGLGALDSPYLLD 106
+ GH+LRTK GGVAAG +D+P+L +
Sbjct: 456 KTAGHLLRTKPESTAMGGVAAGHAVVDTPFLYE 488
>UniRef50_Q9HEY0 Cluster: Glutathione synthetase Gsh1; n=1;
Pneumocystis carinii|Rep: Glutathione synthetase Gsh1 -
Pneumocystis carinii
Length = 235
Score = 33.9 bits (74), Expect = 4.8
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +2
Query: 17 GHMLRTKLADANEGGVAAGLGALDSPYLL 103
G++LRTK D NEGGV G ++DS L+
Sbjct: 206 GYLLRTKDKDKNEGGVMIGCSSIDSCVLI 234
>UniRef50_Q8MTQ0 Cluster: Putative uncharacterized protein; n=1;
Bombyx mori|Rep: Putative uncharacterized protein -
Bombyx mori (Silk moth)
Length = 44
Score = 33.5 bits (73), Expect = 6.4
Identities = 15/18 (83%), Positives = 18/18 (100%)
Frame = -1
Query: 193 MIMLILNAQRSGRVQLVF 140
+I+LILNAQRSGRVQLV+
Sbjct: 10 IIILILNAQRSGRVQLVY 27
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 726,056,999
Number of Sequences: 1657284
Number of extensions: 14125851
Number of successful extensions: 23784
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 23170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23778
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 69143070360
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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