BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0048 (805 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-conse... 48 7e-06 At2g21070.1 68415.m02501 expressed protein 28 8.3 >At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-consensus AT donor splice site at exon 6, AC acceptor splice site at exon 7; identical to Swiss-Prot:P46416 glutathione synthetase, chloroplast precursor (Glutathione synthase) (GSH synthetase) (GSH-S) [Arabidopsis thaliana] Length = 478 Score = 48.0 bits (109), Expect = 7e-06 Identities = 20/33 (60%), Positives = 27/33 (81%) Frame = +2 Query: 5 NRQVGHMLRTKLADANEGGVAAGLGALDSPYLL 103 N Q G+++RTK+A ++EGGVAAG G LDS YL+ Sbjct: 446 NEQSGYLMRTKIASSDEGGVAAGFGVLDSIYLI 478 >At2g21070.1 68415.m02501 expressed protein Length = 483 Score = 27.9 bits (59), Expect = 8.3 Identities = 12/22 (54%), Positives = 15/22 (68%), Gaps = 1/22 (4%) Frame = -2 Query: 255 GWTPYTSCGGVNDENLC-GGEK 193 G P TSCGG +E +C GGE+ Sbjct: 247 GLNPKTSCGGTPEEMVCNGGEQ 268 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,684,899 Number of Sequences: 28952 Number of extensions: 311941 Number of successful extensions: 511 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 473 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 511 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1824072800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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