Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0033
(690 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_46721| Best HMM Match : Tetraspannin (HMM E-Value=4e-35) 53 2e-07
SB_1751| Best HMM Match : No HMM Matches (HMM E-Value=.) 51 8e-07
SB_15887| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06
SB_23554| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06
SB_26670| Best HMM Match : Tetraspannin (HMM E-Value=1.09301e-43) 40 0.002
SB_41644| Best HMM Match : Tetraspannin (HMM E-Value=0) 40 0.003
SB_23671| Best HMM Match : Tetraspannin (HMM E-Value=6.6e-38) 39 0.003
SB_31429| Best HMM Match : Tetraspannin (HMM E-Value=5.9e-35) 37 0.013
SB_22249| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.071
SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0) 34 0.12
SB_31427| Best HMM Match : Tetraspannin (HMM E-Value=0) 34 0.12
SB_22250| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.17
SB_2984| Best HMM Match : Tetraspannin (HMM E-Value=9e-06) 32 0.38
SB_47590| Best HMM Match : TSP_3 (HMM E-Value=7.6e-09) 30 1.5
SB_32096| Best HMM Match : Tetraspannin (HMM E-Value=1.1e-13) 30 2.0
SB_58401| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.7
SB_57558| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.2
SB_38027| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.2
SB_15543| Best HMM Match : DUF1311 (HMM E-Value=6.2) 28 8.2
>SB_46721| Best HMM Match : Tetraspannin (HMM E-Value=4e-35)
Length = 642
Score = 53.2 bits (122), Expect = 2e-07
Identities = 21/56 (37%), Positives = 34/56 (60%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYP 481
CCGA KEN CM+I F + LLI+ E+A + GY+ ++ + ++ +I +YP
Sbjct: 425 CCGAWKENKCMLILFFILLLIVLGLEIAAAVLGYINKDKIQSDLTDDIIRNIKEYP 480
Score = 32.7 bits (71), Expect = 0.29
Identities = 16/58 (27%), Positives = 24/58 (41%), Gaps = 4/58 (6%)
Frame = +1
Query: 514 IQTDLQCCGINSPADWADHG----LPIPSTCCSAQEINDGVVAACTENSTNFHSKGWS 675
+Q D +CCG P+DW +G P +CC ND + + + WS
Sbjct: 490 MQMDFECCGAKGPSDWTFYGQMKYKAGPGSCCGKPN-NDACLPGTRNSKLPRSTHTWS 546
>SB_1751| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 341
Score = 51.2 bits (117), Expect = 8e-07
Identities = 21/56 (37%), Positives = 33/56 (58%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYP 481
CCGA KEN CM+I F FLL++ + E+ G Y +E+ + +++ +I YP
Sbjct: 72 CCGAWKENKCMLICFFAFLLLLLILEIVGGALAYNNKDKIENRLDKDILKAIENYP 127
Score = 38.3 bits (85), Expect = 0.006
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = +3
Query: 99 MALEGGMSCVKYLMFCFNLLFAITGLIILIVGIRAEINSYPYMNFTDENF 248
MAL+G + VK L+ FN +F + GLI+ VGI A Y+ N+
Sbjct: 1 MALQGSSNIVKILVIIFNFIFFLFGLILFGVGIWASTKLGAYVEIASVNY 50
Score = 31.1 bits (67), Expect = 0.88
Identities = 12/42 (28%), Positives = 22/42 (52%), Gaps = 5/42 (11%)
Frame = +1
Query: 514 IQTDLQCCGINSPADWAD-----HGLPIPSTCCSAQEINDGV 624
+QT +CCG ++ DW + +P +CC +++ GV
Sbjct: 137 MQTKFKCCGADNYTDWQSNIKMKNSSSVPDSCCKSEKAGCGV 178
Score = 29.5 bits (63), Expect = 2.7
Identities = 15/42 (35%), Positives = 26/42 (61%), Gaps = 5/42 (11%)
Frame = +1
Query: 514 IQTDLQCCGINSPADW--ADH-GLP--IPSTCCSAQEINDGV 624
+Q ++CCGI + DW +D+ GL +P +CC+ E + G+
Sbjct: 245 MQKTVKCCGITTYEDWKSSDNLGLTDNVPDSCCTTVEKDCGM 286
>SB_15887| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 333
Score = 49.6 bits (113), Expect = 2e-06
Identities = 23/70 (32%), Positives = 42/70 (60%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 493
C GA++EN C++ FSV L IIF +LA+G+ ++ +E ++ L ++T+Y + +
Sbjct: 65 CIGALRENICLLKFFSVVLAIIFFLQLALGVFVFVFQDKVEAVIVEKLQTAVTKYRDNAD 124
Query: 494 VQKTIISYKQ 523
+Q I +Q
Sbjct: 125 LQNLIDGVQQ 134
>SB_23554| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 514
Score = 49.6 bits (113), Expect = 2e-06
Identities = 19/55 (34%), Positives = 32/55 (58%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY 478
CCGA+KE+ C++ TF L II E+ GY+ +E+ + +++N +I Y
Sbjct: 344 CCGAIKEHRCLLATFFALLFIILAVEITGTALGYVYRNKVEEQLGKDMNDTIVSY 398
Score = 31.9 bits (69), Expect = 0.50
Identities = 17/59 (28%), Positives = 26/59 (44%), Gaps = 7/59 (11%)
Frame = +1
Query: 469 YSISGRQERPEDYYIIQTDLQCCGINSPADW-------ADHGLPIPSTCCSAQEINDGV 624
Y G++ + Y IQ +CCGIN DW +H + +P +CC G+
Sbjct: 398 YGEKGKEGITDAYDAIQKKEKCCGINGFKDWQRSYYTKGNHSI-VPDSCCKTVSKGCGI 455
>SB_26670| Best HMM Match : Tetraspannin (HMM E-Value=1.09301e-43)
Length = 211
Score = 39.9 bits (89), Expect = 0.002
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGY 415
C GA KEN CM+ TF V LLI+ E+A + Y
Sbjct: 74 CLGAWKENRCMLGTFFVLLLILLTLEIAAAVLAY 107
Score = 28.7 bits (61), Expect = 4.7
Identities = 13/48 (27%), Positives = 22/48 (45%), Gaps = 5/48 (10%)
Frame = +1
Query: 469 YSISGRQERPEDYYIIQTDLQCCGINSPADWA-----DHGLPIPSTCC 597
Y SG+ + + +Q +CCG+N DW + +P +CC
Sbjct: 138 YGSSGQDGVTKAFDALQEKQKCCGVNGYLDWKASKKYNGTSTVPDSCC 185
Score = 27.9 bits (59), Expect = 8.2
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +3
Query: 111 GGMSCVKYLMFCFNLLFAITGLIILIVGIRAEI 209
G +K L+ FN+LF I G+ ++ VG AE+
Sbjct: 7 GAGRILKILVVFFNVLFFIFGIALIAVGAWAEV 39
>SB_41644| Best HMM Match : Tetraspannin (HMM E-Value=0)
Length = 252
Score = 39.5 bits (88), Expect = 0.003
Identities = 15/55 (27%), Positives = 32/55 (58%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY 478
CCGA+KE+ CM+ T+ + IF E+ + Y+ +E ++ ++ +++ +Y
Sbjct: 77 CCGALKESQCMLGTYFGLVTAIFCLEVTAIVLAYVYRERIEKNLHGDIRSTMNEY 131
Score = 37.9 bits (84), Expect = 0.008
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = +3
Query: 99 MALEGGMSCVKYLMFCFNLLFAITGLIILIVGI 197
M+ + G C+K+++F FNLLF + G I +G+
Sbjct: 1 MSTDWGFKCIKFMVFFFNLLFLLAGCAIFAIGV 33
>SB_23671| Best HMM Match : Tetraspannin (HMM E-Value=6.6e-38)
Length = 919
Score = 39.1 bits (87), Expect = 0.003
Identities = 14/65 (21%), Positives = 34/65 (52%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 493
C +KEN +++++ + L++IF+ E+A G+ + +++ + + + +Y
Sbjct: 78 CVAVIKENRFILVSYLIMLVLIFILEIATGVVAVIYRSEISYELEVGIRGKMNRYGFTDA 137
Query: 494 VQKTI 508
V K I
Sbjct: 138 VTKAI 142
>SB_31429| Best HMM Match : Tetraspannin (HMM E-Value=5.9e-35)
Length = 286
Score = 37.1 bits (82), Expect = 0.013
Identities = 19/71 (26%), Positives = 35/71 (49%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 493
C GA+KEN + ++ +F+ + +A G+ +++ L D R L + Y D +
Sbjct: 91 CVGALKENTLFLASYGIFIFCCLIPIIAAGVLAFLQREKLVDPKAR-LEDGLKSYQQDSS 149
Query: 494 VQKTIISYKQT 526
Q+ I + QT
Sbjct: 150 KQE-FIDFIQT 159
Score = 29.1 bits (62), Expect = 3.6
Identities = 13/32 (40%), Positives = 17/32 (53%), Gaps = 5/32 (15%)
Frame = +1
Query: 514 IQTDLQCCGINSPADWADH-----GLPIPSTC 594
IQT +CCGI S DW + P+P+ C
Sbjct: 157 IQTRFKCCGIESYKDWQANRYFSCSSPVPTAC 188
>SB_22249| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 296
Score = 34.7 bits (76), Expect = 0.071
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 514 IQTDLQCCGINSPADWADHGLPIPSTCCSAQEINDGVVAACTENS 648
+QT+L+CCG+NS DW + I C S + GV +C + S
Sbjct: 165 LQTNLKCCGVNSYTDWQTN---IYFNCSSPGSESCGVPFSCCKES 206
Score = 33.5 bits (73), Expect = 0.17
Identities = 10/25 (40%), Positives = 21/25 (84%)
Frame = +3
Query: 120 SCVKYLMFCFNLLFAITGLIILIVG 194
+C+KY++F N++F + G++IL++G
Sbjct: 37 TCIKYILFFVNVVFWVFGVLILVIG 61
Score = 28.3 bits (60), Expect = 6.2
Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY-PVDK 490
C GA++EN ++ + + + + ++ GIA YM +D V+ L + Y DK
Sbjct: 100 CIGALRENTILLAIYGGAVTLCLIFLVSAGIAVYMH----QDKVVEKLKDDLKHYRDPDK 155
Query: 491 NVQKTIISYKQT 526
+ +I QT
Sbjct: 156 ENFQFLIDRLQT 167
>SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0)
Length = 280
Score = 33.9 bits (74), Expect = 0.12
Identities = 16/71 (22%), Positives = 34/71 (47%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 493
C GA++EN C + + + + + +A G Y + D ++ L + +Y +D
Sbjct: 96 CVGALRENTCFLTVYGTSVFLCLILLIACGAVAYTQ-KDKVQALKERLKQDLKKY-LDDP 153
Query: 494 VQKTIISYKQT 526
+ + +I + QT
Sbjct: 154 LNQDLIDFMQT 164
Score = 32.7 bits (71), Expect = 0.29
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +3
Query: 123 CVKYLMFCFNLLFAITGLIILIVGI 197
C KY +F N++F I GL+++ VG+
Sbjct: 27 CTKYTLFFINVIFFILGLLVMTVGV 51
Score = 31.5 bits (68), Expect = 0.67
Identities = 16/49 (32%), Positives = 25/49 (51%)
Frame = +1
Query: 514 IQTDLQCCGINSPADWADHGLPIPSTCCSAQEINDGVVAACTENSTNFH 660
+QT L+CCG+ S DW + C S ++ GV +C + S F+
Sbjct: 162 MQTLLKCCGVESYKDWQANPY---FNCSSPGRMSCGVPFSCCKESIQFN 207
>SB_31427| Best HMM Match : Tetraspannin (HMM E-Value=0)
Length = 299
Score = 33.9 bits (74), Expect = 0.12
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 514 IQTDLQCCGINSPADWADHGLPIPSTCCSAQEINDGVVAACTENS 648
+QT+L+CCGINS DW + + C S + GV +C + S
Sbjct: 165 LQTNLKCCGINSYNDWQTN---VYFNCSSPGSESCGVPFSCCKES 206
Score = 33.5 bits (73), Expect = 0.17
Identities = 10/25 (40%), Positives = 21/25 (84%)
Frame = +3
Query: 120 SCVKYLMFCFNLLFAITGLIILIVG 194
+C+KY++F N++F + G++IL++G
Sbjct: 37 TCIKYILFFVNVVFWVFGVLILVIG 61
>SB_22250| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 313
Score = 33.5 bits (73), Expect = 0.17
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3
Query: 123 CVKYLMFCFNLLFAITGLIILIVGI 197
CVKY +F N++F + G +IL VGI
Sbjct: 59 CVKYSLFTINVIFWLAGAVILAVGI 83
Score = 31.5 bits (68), Expect = 0.67
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = +2
Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY 478
C GA++EN C++ + II + G G+ LE V L +I Y
Sbjct: 122 CIGALRENRCLLTAYIWMCGIILAGMIVCGGLGFYYKDVLETKVTAQLKDAIVLY 176
Score = 29.5 bits (63), Expect = 2.7
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = +1
Query: 514 IQTDLQCCGINSPADW 561
+QT+LQCCG+ DW
Sbjct: 191 VQTELQCCGVQGLNDW 206
>SB_2984| Best HMM Match : Tetraspannin (HMM E-Value=9e-06)
Length = 187
Score = 32.3 bits (70), Expect = 0.38
Identities = 12/44 (27%), Positives = 25/44 (56%)
Frame = +2
Query: 350 ITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYP 481
+ F V L ++F+ EL I GY+ ++++ + L+ ++ YP
Sbjct: 130 LQFGVILGLLFLVELGTAITGYIFRSEIKTGLSDGLDTALKDYP 173
>SB_47590| Best HMM Match : TSP_3 (HMM E-Value=7.6e-09)
Length = 669
Score = 30.3 bits (65), Expect = 1.5
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +3
Query: 114 GMSCVKYLMFCFNLLFAITGLI 179
GM C+KYL+F FN +F + +I
Sbjct: 76 GMQCMKYLLFAFNFVFWCSKII 97
Score = 29.1 bits (62), Expect = 3.6
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +2
Query: 314 CCGAVKENHCMIITFS 361
CCGAVKEN C ++ S
Sbjct: 217 CCGAVKENKCFLLLVS 232
>SB_32096| Best HMM Match : Tetraspannin (HMM E-Value=1.1e-13)
Length = 174
Score = 29.9 bits (64), Expect = 2.0
Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 6/37 (16%)
Frame = +1
Query: 514 IQTDLQCCGINS-PADWADH-----GLPIPSTCCSAQ 606
IQ LQCCG +S P DW ++ +P +CC +
Sbjct: 90 IQERLQCCGADSGPNDWMNNKHFLQSASVPDSCCKQE 126
>SB_58401| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 426
Score = 29.5 bits (63), Expect = 2.7
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +2
Query: 314 CCGAVKENHCMIIT 355
CCGAVKE+ CM+ T
Sbjct: 332 CCGAVKESRCMLAT 345
>SB_57558| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 733
Score = 28.3 bits (60), Expect = 6.2
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -3
Query: 211 FISALMPTIRMINPVMANRRLKQNMRYF 128
FI+ALM I +I +M + L++ M YF
Sbjct: 39 FITALMGNIILITVIMKTKSLRRTMNYF 66
>SB_38027| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 316
Score = 28.3 bits (60), Expect = 6.2
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +2
Query: 317 CGAVKENHCMIITFSVFLLIIFVAELAVGI 406
C + N C++ T+ F+LI+ VAE+ + +
Sbjct: 38 CALLYMNPCLLKTYFSFMLILVVAEVTIAV 67
>SB_15543| Best HMM Match : DUF1311 (HMM E-Value=6.2)
Length = 163
Score = 27.9 bits (59), Expect = 8.2
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = -1
Query: 531 LQVCLYDIIVFWTFLSTGY*VMEAFRLRITESSKSVCF 418
+QVCLY +FW S+G V + + + SV F
Sbjct: 4 IQVCLYFTFIFWNGFSSGEDVQQNIAGVVYHKTDSVLF 41
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,039,404
Number of Sequences: 59808
Number of extensions: 428847
Number of successful extensions: 1090
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1027
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1090
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1793485733
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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