Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0031
(821 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10850.1 68416.m01307 hydroxyacylglutathione hydrolase, cytop... 81 1e-15
At2g31350.2 68415.m03830 hydroxyacylglutathione hydrolase, putat... 52 5e-07
At2g31350.1 68415.m03829 hydroxyacylglutathione hydrolase, putat... 52 5e-07
At1g06130.2 68414.m00643 hydroxyacylglutathione hydrolase, putat... 50 2e-06
At1g06130.1 68414.m00642 hydroxyacylglutathione hydrolase, putat... 50 2e-06
At2g43430.2 68415.m05397 hydroxyacylglutathione hydrolase, mitoc... 49 3e-06
At2g43430.1 68415.m05398 hydroxyacylglutathione hydrolase, mitoc... 49 3e-06
At5g27700.1 68418.m03322 40S ribosomal protein S21 (RPS21C) ribo... 29 2.8
At4g04530.1 68417.m00660 Ulp1 protease family protein (snoR29) c... 29 2.8
At3g53890.1 68416.m05953 40S ribosomal protein S21 (RPS21B) ribo... 29 2.8
>At3g10850.1 68416.m01307 hydroxyacylglutathione hydrolase,
cytoplasmic / glyoxalase II (GLX2-2) identical to
SP|O24496 Hydroxyacylglutathione hydrolase cytoplasmic
(EC 3.1.2.6) (Glyoxalase II) (Glx II) {Arabidopsis
thaliana}
Length = 258
Score = 80.6 bits (190), Expect = 1e-15
Identities = 54/181 (29%), Positives = 87/181 (48%), Gaps = 2/181 (1%)
Frame = +3
Query: 261 MDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXXA 440
M + +P LQDNY YLI+D++T +AA+VDPV+P+ V+ + E+ + A
Sbjct: 1 MKIFHVPCLQDNYSYLIIDESTGDAAVVDPVDPEKVIASAEKHQAKIKFVLTTHHHWDHA 60
Query: 441 GGNEDLIKERPGLIVYGGD-DRIGPSQKKLSTIQSLKSVT*MYSVFLHPVIQLGIYVIS* 617
GGNE + + P + VYGG D++ + L + + LH ++
Sbjct: 61 GGNEKIKQLVPDIKVYGGSLDKVKGCTDAVDNGDKLTLGQDINILALHTPCHTKGHISYY 120
Query: 618 LLQKKVTIQLSSPGDTLFLGGCGQVL-*RARQIKCTKH*QF*AAA*PH*MFCGHEYTLAG 794
+ K+ GDTLF+ GCG+ A Q+ + A P ++CGHEYT+
Sbjct: 121 VNGKEGENPAVFTGDTLFVAGCGKFFEGTAEQMYQSLCVTLAALPKPTQVYCGHEYTVKN 180
Query: 795 I 797
+
Sbjct: 181 L 181
>At2g31350.2 68415.m03830 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme [Arabidopsis thaliana] gi|2570338|gb|AAC49865
Length = 323
Score = 52.0 bits (119), Expect = 5e-07
Identities = 25/80 (31%), Positives = 44/80 (55%)
Frame = +3
Query: 252 VKNMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXX 431
V ++ ++++P L+DNY Y++ D+ T +VDP E + ++ +++ G NL
Sbjct: 67 VSSLQIELVPCLKDNYAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHY 126
Query: 432 XXAGGNEDLIKERPGLIVYG 491
GGN +L K+R G V G
Sbjct: 127 DHTGGNLEL-KDRYGAKVIG 145
>At2g31350.1 68415.m03829 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme [Arabidopsis thaliana] gi|2570338|gb|AAC49865
Length = 324
Score = 52.0 bits (119), Expect = 5e-07
Identities = 25/80 (31%), Positives = 44/80 (55%)
Frame = +3
Query: 252 VKNMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXX 431
V ++ ++++P L+DNY Y++ D+ T +VDP E + ++ +++ G NL
Sbjct: 68 VSSLQIELVPCLKDNYAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHY 127
Query: 432 XXAGGNEDLIKERPGLIVYG 491
GGN +L K+R G V G
Sbjct: 128 DHTGGNLEL-KDRYGAKVIG 146
>At1g06130.2 68414.m00643 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme GB:AAC49865 GI:2570338 from [Arabidopsis
thaliana]
Length = 330
Score = 50.0 bits (114), Expect = 2e-06
Identities = 52/187 (27%), Positives = 76/187 (40%), Gaps = 3/187 (1%)
Frame = +3
Query: 258 NMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXX 437
++ ++++P L DNY Y++ D+ T +VDP E V+ A+++ NL
Sbjct: 74 SLQIELVPCLTDNYAYILHDEDTGTVGVVDPSEAVPVMDALQKNSRNLTYILNTHHHYDH 133
Query: 438 AGGNEDLIKERPGLIVYGG---DDRIGPSQKKLSTIQSLKSVT*MYSVFLHPVIQLGIYV 608
GGN +L K+R G V G DRI L + P G
Sbjct: 134 TGGNLEL-KDRYGAKVIGSAADRDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRG--H 190
Query: 609 IS*LLQKKVTIQLSSPGDTLFLGGCGQVL*RARQIKCTKH*QF*AAA*PH*MFCGHEYTL 788
IS I GDTLF CG++ + + A ++CGHEYTL
Sbjct: 191 ISFYFPGARAI---FTGDTLFSLSCGKLFEGTPEQMLASLQRIIALPDDTSVYCGHEYTL 247
Query: 789 AGIXKFA 809
+ KFA
Sbjct: 248 SN-SKFA 253
Score = 27.9 bits (59), Expect = 8.6
Identities = 19/64 (29%), Positives = 27/64 (42%)
Frame = +2
Query: 539 KFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSVVFTGGYTLLRRMWAGSLKGTADQM 718
K+ V + TP HT GHI ++ +FTG TL +GT +QM
Sbjct: 170 KWMFAGHEVHIMETPGHTRGHISFYFPGAR-----AIFTGD-TLFSLSCGKLFEGTPEQM 223
Query: 719 YKAL 730
+L
Sbjct: 224 LASL 227
>At1g06130.1 68414.m00642 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme GB:AAC49865 GI:2570338 from [Arabidopsis
thaliana]
Length = 331
Score = 50.0 bits (114), Expect = 2e-06
Identities = 52/187 (27%), Positives = 76/187 (40%), Gaps = 3/187 (1%)
Frame = +3
Query: 258 NMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXX 437
++ ++++P L DNY Y++ D+ T +VDP E V+ A+++ NL
Sbjct: 75 SLQIELVPCLTDNYAYILHDEDTGTVGVVDPSEAVPVMDALQKNSRNLTYILNTHHHYDH 134
Query: 438 AGGNEDLIKERPGLIVYGG---DDRIGPSQKKLSTIQSLKSVT*MYSVFLHPVIQLGIYV 608
GGN +L K+R G V G DRI L + P G
Sbjct: 135 TGGNLEL-KDRYGAKVIGSAADRDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRG--H 191
Query: 609 IS*LLQKKVTIQLSSPGDTLFLGGCGQVL*RARQIKCTKH*QF*AAA*PH*MFCGHEYTL 788
IS I GDTLF CG++ + + A ++CGHEYTL
Sbjct: 192 ISFYFPGARAI---FTGDTLFSLSCGKLFEGTPEQMLASLQRIIALPDDTSVYCGHEYTL 248
Query: 789 AGIXKFA 809
+ KFA
Sbjct: 249 SN-SKFA 254
Score = 27.9 bits (59), Expect = 8.6
Identities = 19/64 (29%), Positives = 27/64 (42%)
Frame = +2
Query: 539 KFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSVVFTGGYTLLRRMWAGSLKGTADQM 718
K+ V + TP HT GHI ++ +FTG TL +GT +QM
Sbjct: 171 KWMFAGHEVHIMETPGHTRGHISFYFPGAR-----AIFTGD-TLFSLSCGKLFEGTPEQM 224
Query: 719 YKAL 730
+L
Sbjct: 225 LASL 228
>At2g43430.2 68415.m05397 hydroxyacylglutathione hydrolase,
mitochondrial / glyoxalase II (GLX2-1) identical to
SP|O24495 Hydroxyacylglutathione hydrolase,
mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II)
(Glx II) {Arabidopsis thaliana}
Length = 313
Score = 49.2 bits (112), Expect = 3e-06
Identities = 26/78 (33%), Positives = 41/78 (52%)
Frame = +3
Query: 258 NMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXX 437
++ ++++P +DNY YL+ D+ T +VDP E V++A+ + NL
Sbjct: 59 SLKIELVPCSKDNYAYLLHDEDTGTVGVVDPSEAAPVIEALSRKNWNLTYILNTHHHDDH 118
Query: 438 AGGNEDLIKERPGLIVYG 491
GGN +L KER G V G
Sbjct: 119 IGGNAEL-KERYGAKVIG 135
Score = 31.9 bits (69), Expect = 0.53
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 1/82 (1%)
Frame = +2
Query: 524 VEHNTKFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSVVFTGGYTLLRRMWAGSL-K 700
++ + K+ V+ L TP HT GHI ++ + +FTG L+ + G+L +
Sbjct: 150 LKDSDKWMFAGHEVRILDTPGHTQGHISFYFP-----GSATIFTG--DLIYSLSCGTLSE 202
Query: 701 GTADQMYKALTILSSCLTTLNV 766
GT +QM +L + S N+
Sbjct: 203 GTPEQMLSSLQKIVSLPDDTNI 224
>At2g43430.1 68415.m05398 hydroxyacylglutathione hydrolase,
mitochondrial / glyoxalase II (GLX2-1) identical to
SP|O24495 Hydroxyacylglutathione hydrolase,
mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II)
(Glx II) {Arabidopsis thaliana}
Length = 331
Score = 49.2 bits (112), Expect = 3e-06
Identities = 26/78 (33%), Positives = 41/78 (52%)
Frame = +3
Query: 258 NMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXX 437
++ ++++P +DNY YL+ D+ T +VDP E V++A+ + NL
Sbjct: 77 SLKIELVPCSKDNYAYLLHDEDTGTVGVVDPSEAAPVIEALSRKNWNLTYILNTHHHDDH 136
Query: 438 AGGNEDLIKERPGLIVYG 491
GGN +L KER G V G
Sbjct: 137 IGGNAEL-KERYGAKVIG 153
Score = 31.9 bits (69), Expect = 0.53
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 1/82 (1%)
Frame = +2
Query: 524 VEHNTKFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSVVFTGGYTLLRRMWAGSL-K 700
++ + K+ V+ L TP HT GHI ++ + +FTG L+ + G+L +
Sbjct: 168 LKDSDKWMFAGHEVRILDTPGHTQGHISFYFP-----GSATIFTG--DLIYSLSCGTLSE 220
Query: 701 GTADQMYKALTILSSCLTTLNV 766
GT +QM +L + S N+
Sbjct: 221 GTPEQMLSSLQKIVSLPDDTNI 242
>At5g27700.1 68418.m03322 40S ribosomal protein S21 (RPS21C)
ribosomal protein S21, Zea mays, PIR:T03945
Length = 85
Score = 29.5 bits (63), Expect = 2.8
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +2
Query: 503 DRALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSV 646
+R +T K + + IG+L+ L+T TT +C FV A + + V
Sbjct: 21 NRLITSKDHASVQLNIGHLDANGLYTGQFTTFALCGFVRAQGDADSGV 68
>At4g04530.1 68417.m00660 Ulp1 protease family protein (snoR29)
contains Pfam profile PF02902: Ulp1 protease family,
C-terminal catalytic domain; snoR29 gene for small
nucleolar RNA GI:15706258
Length = 917
Score = 29.5 bits (63), Expect = 2.8
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = +3
Query: 249 PVKNMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQ 389
P KN+D KI+P Q N I D ++K A + +L A+++Q
Sbjct: 479 PAKNLDPKIVPERQSNMPSRIADSSSKNPAPSGQTHAQ-LLNALQQQ 524
>At3g53890.1 68416.m05953 40S ribosomal protein S21 (RPS21B)
ribosomal protein S21, cytosolic - Oryza sativa,
PIR:S38357
Length = 82
Score = 29.5 bits (63), Expect = 2.8
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +2
Query: 503 DRALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSV 646
+R +T K + + IG+L+ L+T TT +C FV A + + V
Sbjct: 21 NRMITSKDHASVQLNIGHLDANGLYTGQFTTFALCGFVRAQGDADSGV 68
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,563,132
Number of Sequences: 28952
Number of extensions: 326640
Number of successful extensions: 751
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 724
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 743
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1882599200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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