Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0023
(765 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 146 1e-35
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 146 1e-35
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 146 1e-35
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.84
At1g29760.1 68414.m03639 expressed protein 31 0.84
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 3.4
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 146 bits (355), Expect = 1e-35
Identities = 66/85 (77%), Positives = 75/85 (88%)
Frame = +1
Query: 1 HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 180
HLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Q
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQ 67
Query: 181 RLIKVDGKVRTDPTYPAGFMDVVSM 255
R I+VDGKVRTD TYPAGFMDVVS+
Sbjct: 68 RHIQVDGKVRTDKTYPAGFMDVVSI 92
Score = 126 bits (305), Expect = 1e-29
Identities = 56/88 (63%), Positives = 69/88 (78%)
Frame = +3
Query: 258 KTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPL 437
KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPL
Sbjct: 94 KTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPL 153
Query: 438 IKVNDSIQLDIATTKIMDFIKFESGTCV 521
IK ND+I+LD+ KI++FIKF+ G V
Sbjct: 154 IKPNDTIKLDLEANKIVEFIKFDVGNVV 181
Score = 102 bits (244), Expect = 3e-22
Identities = 48/86 (55%), Positives = 62/86 (72%)
Frame = +2
Query: 506 VGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKARRRNI 685
VGN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK + +
Sbjct: 177 VGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWV 236
Query: 686 SLPRGKGXXXXXXXXERDKRIRSQRS 763
SLP+GKG R KR+ SQ++
Sbjct: 237 SLPKGKGIKLTIIEEAR-KRLASQQA 261
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 146 bits (355), Expect = 1e-35
Identities = 66/85 (77%), Positives = 75/85 (88%)
Frame = +1
Query: 1 HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 180
HLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Q
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQ 67
Query: 181 RLIKVDGKVRTDPTYPAGFMDVVSM 255
R I+VDGKVRTD TYPAGFMDVVS+
Sbjct: 68 RHIQVDGKVRTDKTYPAGFMDVVSI 92
Score = 126 bits (304), Expect = 2e-29
Identities = 56/88 (63%), Positives = 69/88 (78%)
Frame = +3
Query: 258 KTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPL 437
KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPL
Sbjct: 94 KTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPL 153
Query: 438 IKVNDSIQLDIATTKIMDFIKFESGTCV 521
IK ND+I+LD+ KI++FIKF+ G V
Sbjct: 154 IKPNDTIKLDLEENKIVEFIKFDVGNVV 181
Score = 102 bits (244), Expect = 3e-22
Identities = 48/86 (55%), Positives = 62/86 (72%)
Frame = +2
Query: 506 VGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKARRRNI 685
VGN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK + +
Sbjct: 177 VGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWV 236
Query: 686 SLPRGKGXXXXXXXXERDKRIRSQRS 763
SLP+GKG R KR+ SQ++
Sbjct: 237 SLPKGKGIKLTIIEEAR-KRLASQQA 261
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 146 bits (355), Expect = 1e-35
Identities = 66/85 (77%), Positives = 75/85 (88%)
Frame = +1
Query: 1 HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 180
HLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Q
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQ 67
Query: 181 RLIKVDGKVRTDPTYPAGFMDVVSM 255
R I+VDGKVRTD TYPAGFMDVVS+
Sbjct: 68 RHIQVDGKVRTDKTYPAGFMDVVSI 92
Score = 126 bits (304), Expect = 2e-29
Identities = 56/88 (63%), Positives = 69/88 (78%)
Frame = +3
Query: 258 KTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPL 437
KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPL
Sbjct: 94 KTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPL 153
Query: 438 IKVNDSIQLDIATTKIMDFIKFESGTCV 521
IK ND+I+LD+ KI++FIKF+ G V
Sbjct: 154 IKPNDTIKLDLEENKIVEFIKFDVGNVV 181
Score = 101 bits (241), Expect = 7e-22
Identities = 47/86 (54%), Positives = 62/86 (72%)
Frame = +2
Query: 506 VGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKARRRNI 685
VGN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK + +
Sbjct: 177 VGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWV 236
Query: 686 SLPRGKGXXXXXXXXERDKRIRSQRS 763
SLP+GKG R KR+ +Q++
Sbjct: 237 SLPKGKGIKLTIIEEAR-KRLSAQQA 261
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.84
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 104 KHSRNLWGPVDGLGAYTPPSLSNIHAL 24
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.84
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -2
Query: 215 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 123
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 3.4
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -2
Query: 194 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 78
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,591,455
Number of Sequences: 28952
Number of extensions: 390545
Number of successful extensions: 1173
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1172
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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