Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0011
(689 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 88 1e-18
SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces pom... 56 4e-09
SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 51 2e-07
SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual 47 3e-06
SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 38 0.002
SPBC1709.07 |erg27||3-keto sterol reductase |Schizosaccharomyces... 35 0.010
SPAC4G9.15 |||ketoreductase |Schizosaccharomyces pombe|chr 1|||M... 33 0.039
SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces p... 32 0.068
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 31 0.12
SPBC2F12.15c |||palmitoyltransferase|Schizosaccharomyces pombe|c... 29 0.63
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 28 1.5
SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces pomb... 27 2.6
SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription te... 25 7.8
SPAC4G9.08c |rpc2||DNA-directed RNA polymerase III complex subun... 25 7.8
>SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 339
Score = 88.2 bits (209), Expect = 1e-18
Identities = 47/129 (36%), Positives = 73/129 (56%)
Frame = +1
Query: 244 WTMLSTVKLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCN 423
WT L GK AL+TG + GIG TAL+ ++GA+V +A R+ E+ ++ I
Sbjct: 32 WTFNDIPDLTGKVALVTGSSGGIGYVTALELARKGAKVYLAGRNEEKYQKVMKQIHDEVR 91
Query: 424 NINETGHLVIAKCDLTSLKSVREFAQNIWIPNHKFNILVNNAGVMMCPKGETEDGFEIQF 603
+ + + DL +SV + A++ K +ILVNNAG+M P T+DG+E+Q
Sbjct: 92 H----SKIRFLRLDLLDFESVYQAAESFIAKEEKLHILVNNAGIMNPPFELTKDGYELQI 147
Query: 604 ATNHLAHFL 630
TN+L+H+L
Sbjct: 148 QTNYLSHYL 156
>SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 397
Score = 56.4 bits (130), Expect = 4e-09
Identities = 43/144 (29%), Positives = 72/144 (50%), Gaps = 10/144 (6%)
Frame = +1
Query: 232 AFI*WTMLSTV----KLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAK 399
AF WT T K+ G ++TG ++GIG+ GA+V++ R+ ++
Sbjct: 57 AFKFWTSGRTTTWERKMNGMVVMVTGGSSGIGQVVVEKLASLGAQVVILLRT--EPDQFT 114
Query: 400 ADIEKSCNNINETGHLVIAKCDLTSLKSVREFAQNIWI---PNHKFNILVNNAGVMMCP- 567
D + + CDL+S+ SVR+FA WI P + +++V +GV++ P
Sbjct: 115 VDYIMDLRKRTKNQLIYTEVCDLSSMLSVRKFATK-WIDCTPIRRLDMIVLCSGVLLPPF 173
Query: 568 --KGETEDGFEIQFATNHLAHFLL 633
+ TE+G E+Q+ATN L + L
Sbjct: 174 MDRQTTEEGVELQWATNFLGPYQL 197
>SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 259
Score = 50.8 bits (116), Expect = 2e-07
Identities = 33/100 (33%), Positives = 54/100 (54%), Gaps = 1/100 (1%)
Frame = +1
Query: 265 KLVGKTALITGCNTGIGKETALDFYK-RGARVIMACRSLERAEEAKADIEKSCNNINETG 441
+L GKT LITG ++GIGK TA + K ++I+A R EE ++E E
Sbjct: 3 RLDGKTILITGASSGIGKSTAFEIAKVAKVKLILAARRFSTVEEIAKELESK----YEVS 58
Query: 442 HLVIAKCDLTSLKSVREFAQNIWIPNHKFNILVNNAGVMM 561
L + K D++ LKS+ +++ ++L+NNAG+ +
Sbjct: 59 VLPL-KLDVSDLKSIPGVIESLPKEFADIDVLINNAGLAL 97
>SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual
Length = 503
Score = 46.8 bits (106), Expect = 3e-06
Identities = 38/116 (32%), Positives = 60/116 (51%), Gaps = 4/116 (3%)
Frame = +1
Query: 286 LITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGHLVIAKCD 465
L+TG + G+GK A + RGA V + R++ + +EA A++ S I+E + D
Sbjct: 26 LVTGGSQGLGKAIAKELVLRGANVTIVARTVTKLQEAVAELSDS--KIHEDQQVSFESVD 83
Query: 466 LTSLKSVREFAQNI-WIPNHKFNILVNNAGVMMCPKGETE---DGFEIQFATNHLA 621
LTS +SV + + + P+H +V+ AG + P TE FE Q N+LA
Sbjct: 84 LTSYESVHSMIERLPFCPDH----VVHCAGSCI-PGFFTELDPSVFEKQMRQNYLA 134
>SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 286
Score = 37.5 bits (83), Expect = 0.002
Identities = 21/56 (37%), Positives = 33/56 (58%), Gaps = 2/56 (3%)
Frame = +1
Query: 265 KLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLER--AEEAKADIEKSCNN 426
KL K L+TG ++GIGK A+ F + G+ ++++C ER AE + IE+ N
Sbjct: 39 KLAEKKTLLTGGDSGIGKAAAVMFAREGSDLVISCLPEERDDAEVTRDLIEREGRN 94
>SPBC1709.07 |erg27||3-keto sterol reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 338
Score = 35.1 bits (77), Expect = 0.010
Identities = 33/102 (32%), Positives = 50/102 (49%), Gaps = 10/102 (9%)
Frame = +1
Query: 277 KTALITGCNTGIGKETA---LDFYKRGAR-------VIMACRSLERAEEAKADIEKSCNN 426
K ALITG N+G+G A L FY+ + VI+ CRS E+AE+A +++
Sbjct: 5 KYALITGSNSGLGFGIATRLLQFYQPRLQDEPEVFTVILTCRSREKAEDACRRLKEFFP- 63
Query: 427 INETGHLVIAKCDLTSLKSVREFAQNIWIPNHKFNILVNNAG 552
+ L DL+++ SV Q+I K + + NAG
Sbjct: 64 -DRKIRLEYVLLDLSNMASVEAAVQDIATRFPKLDFVYLNAG 104
>SPAC4G9.15 |||ketoreductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 33.1 bits (72), Expect = 0.039
Identities = 23/93 (24%), Positives = 41/93 (44%)
Frame = +1
Query: 274 GKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGHLVI 453
G A++TG GIGKE A G V++ R+ E+ + ++E T +
Sbjct: 57 GYWAVVTGATDGIGKEYATQLAMSGFNVVLISRTQEKLDALAKELETVAKVKTRTIAIDY 116
Query: 454 AKCDLTSLKSVREFAQNIWIPNHKFNILVNNAG 552
K T+ ++ + Q+ + +L+NN G
Sbjct: 117 TK---TTAETFEKLHQD--LVGTPITVLINNVG 144
>SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 254
Score = 32.3 bits (70), Expect = 0.068
Identities = 28/95 (29%), Positives = 44/95 (46%), Gaps = 1/95 (1%)
Frame = +1
Query: 274 GKTALITGCNTGIGKETALDFY-KRGARVIMACRSLERAEEAKADIEKSCNNINETGHLV 450
G +I G N GIG + K G V + R A E K D K+ +N++
Sbjct: 6 GIVYVIVGGNRGIGLSLVKELSNKEGVTVFASARGPGSASELK-DWSKTHSNVH------ 58
Query: 451 IAKCDLTSLKSVREFAQNIWIPNHKFNILVNNAGV 555
I K D+TSL+S ++ A + ++L N+G+
Sbjct: 59 IIKLDVTSLRSAKDAAMQVEKVVKCIDVLWVNSGI 93
>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 255
Score = 31.5 bits (68), Expect = 0.12
Identities = 30/113 (26%), Positives = 52/113 (46%), Gaps = 1/113 (0%)
Frame = +1
Query: 250 MLSTVKLVGKTALITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNI 429
M S L GKT LITG + GIG A F G+ V + ++A E A++ + + +
Sbjct: 1 MTSMFSLKGKTTLITGGSGGIGFSIAKAFAAAGSNVGLLYGRNKKALEYAAEL-RDKHGV 59
Query: 430 NETGHLVIAKCDLTSLKSVREFA-QNIWIPNHKFNILVNNAGVMMCPKGETED 585
+ C + + +V E Q + + ++++ NAG+ + P ED
Sbjct: 60 QAKAY----SCPIENRSAVIETTNQAVEELGGRLDVMIANAGIAI-PHLSLED 107
>SPBC2F12.15c |||palmitoyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 329
Score = 29.1 bits (62), Expect = 0.63
Identities = 15/61 (24%), Positives = 27/61 (44%)
Frame = +3
Query: 486 TRICTKHLDTEPQIQYPC*QCRSDDVSQGRN*RWFRNTVCYKPFSSFSFKHCCFYLEFET 665
+R C K + + + C QC + + WF+N V ++ F + CFYL +
Sbjct: 96 SRFCEKCQEYKCDRSHHCSQCNKCILRMDHHCMWFKNCVGFRNHKFFFLE--CFYLNLYS 153
Query: 666 V 668
+
Sbjct: 154 I 154
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/72 (20%), Positives = 36/72 (50%)
Frame = +1
Query: 286 LITGCNTGIGKETALDFYKRGARVIMACRSLERAEEAKADIEKSCNNINETGHLVIAKCD 465
L+ + GI +T +FY ++ ++ L + + AD++++ + +T + ++K
Sbjct: 153 LLVDASQGIQAQTLSNFYMAFSQNLVIIPVLNKVDLPTADVDRTLIQVQQTFDIPMSKPI 212
Query: 466 LTSLKSVREFAQ 501
L S K+ + Q
Sbjct: 213 LVSSKTGKNVEQ 224
>SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 247
Score = 27.1 bits (57), Expect = 2.6
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = +1
Query: 277 KTALITGCNTGIGKETALDFYKRGARVIMACRSL 378
K L+TG + GIG TA K+ A+VI RSL
Sbjct: 7 KVILLTGSSKGIGLATAEALQKK-AKVIAVSRSL 39
>SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription
termination factor Reb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 504
Score = 25.4 bits (53), Expect = 7.8
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +1
Query: 466 LTSLKSVREFAQNIWIPNHKFNILVNNAG 552
LT S E +N+W+ ++ L+NN G
Sbjct: 417 LTKAASKFELQENVWLLERIYDSLLNNGG 445
>SPAC4G9.08c |rpc2||DNA-directed RNA polymerase III complex subunit
Rpc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1165
Score = 25.4 bits (53), Expect = 7.8
Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Frame = +3
Query: 222 YLKCLYLVDDVKHSKVG--R*NCTDHWLQHRNREGN-RSGFLQKRCSCYYGMSQL 377
Y K ++V D H++ R T + R+R+G R G +++ C YG SQL
Sbjct: 1040 YQKLKHMVMDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIAYGASQL 1094
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,793,520
Number of Sequences: 5004
Number of extensions: 56155
Number of successful extensions: 146
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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