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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0002
         (431 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q24524 Cluster: Protein singed; n=14; Endopterygota|Rep...    91   7e-18
UniRef50_A7RUD3 Cluster: Predicted protein; n=1; Nematostella ve...    50   2e-05
UniRef50_Q4RZ67 Cluster: Chromosome undetermined SCAF14961, whol...    50   3e-05
UniRef50_Q4RZ66 Cluster: Chromosome undetermined SCAF14961, whol...    50   3e-05
UniRef50_Q6PBS5 Cluster: Fascin homolog 1-like, actin-bundling p...    49   5e-05
UniRef50_Q16658 Cluster: Fascin; n=49; Euteleostomi|Rep: Fascin ...    48   1e-04
UniRef50_UPI0000E5925D Cluster: fascin 2 isoform 2; n=1; Homo sa...    47   1e-04
UniRef50_Q05634 Cluster: Fascin; n=1; Strongylocentrotus purpura...    44   0.001
UniRef50_Q7NI81 Cluster: Glr2302 protein; n=1; Gloeobacter viola...    32   4.5  
UniRef50_Q1IJA0 Cluster: Type IV pilus assembly PilZ; n=2; Acido...    32   5.9  
UniRef50_A2EE67 Cluster: Surface antigen BspA-like; n=1; Trichom...    31   7.8  
UniRef50_Q04893 Cluster: Uncharacterized protein YMR317W; n=1; S...    31   7.8  

>UniRef50_Q24524 Cluster: Protein singed; n=14; Endopterygota|Rep:
           Protein singed - Drosophila melanogaster (Fruit fly)
          Length = 512

 Score = 91.5 bits (217), Expect = 7e-18
 Identities = 40/57 (70%), Positives = 48/57 (84%)
 Frame = +3

Query: 261 MNGHSNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQ 431
           MNG      +S GDI++QNQQKGWWTIGLIN +++Y+TAETFGFK+NANG SLKKKQ
Sbjct: 1   MNGQGCELGHSNGDIISQNQQKGWWTIGLINGQHKYMTAETFGFKLNANGASLKKKQ 57


>UniRef50_A7RUD3 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 488

 Score = 50.4 bits (115), Expect = 2e-05
 Identities = 22/34 (64%), Positives = 28/34 (82%), Gaps = 1/34 (2%)
 Frame = +3

Query: 333 WTIGLINSRY-RYLTAETFGFKINANGTSLKKKQ 431
           W IGL+N    +YLTAETFGFK+N++G SL+KKQ
Sbjct: 7   WNIGLMNDNSSKYLTAETFGFKLNSDGVSLRKKQ 40


>UniRef50_Q4RZ67 Cluster: Chromosome undetermined SCAF14961, whole
           genome shotgun sequence; n=1; Tetraodon
           nigroviridis|Rep: Chromosome undetermined SCAF14961,
           whole genome shotgun sequence - Tetraodon nigroviridis
           (Green puffer)
          Length = 325

 Score = 49.6 bits (113), Expect = 3e-05
 Identities = 29/53 (54%), Positives = 35/53 (66%)
 Frame = +3

Query: 273 SNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQ 431
           +NG  N   D+L Q Q       GLIN   +YLTAETFGFKINA+ +S+KKKQ
Sbjct: 3   TNGASNGTSDML-QIQ------FGLINCGNKYLTAETFGFKINASASSMKKKQ 48


>UniRef50_Q4RZ66 Cluster: Chromosome undetermined SCAF14961, whole
           genome shotgun sequence; n=1; Tetraodon
           nigroviridis|Rep: Chromosome undetermined SCAF14961,
           whole genome shotgun sequence - Tetraodon nigroviridis
           (Green puffer)
          Length = 353

 Score = 49.6 bits (113), Expect = 3e-05
 Identities = 29/53 (54%), Positives = 35/53 (66%)
 Frame = +3

Query: 273 SNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQ 431
           +NG  N   D+L Q Q       GLIN   +YLTAETFGFKINA+ +S+KKKQ
Sbjct: 3   TNGASNGTSDML-QIQ------FGLINCGNKYLTAETFGFKINASASSMKKKQ 48


>UniRef50_Q6PBS5 Cluster: Fascin homolog 1-like, actin-bundling
           protein; n=10; Gnathostomata|Rep: Fascin homolog 1-like,
           actin-bundling protein - Danio rerio (Zebrafish)
           (Brachydanio rerio)
          Length = 290

 Score = 48.8 bits (111), Expect = 5e-05
 Identities = 22/30 (73%), Positives = 24/30 (80%)
 Frame = +3

Query: 342 GLINSRYRYLTAETFGFKINANGTSLKKKQ 431
           GLIN   RYLTAE FGFK+NA+  SLKKKQ
Sbjct: 14  GLINHENRYLTAEAFGFKVNASAPSLKKKQ 43


>UniRef50_Q16658 Cluster: Fascin; n=49; Euteleostomi|Rep: Fascin -
           Homo sapiens (Human)
          Length = 493

 Score = 47.6 bits (108), Expect = 1e-04
 Identities = 21/30 (70%), Positives = 25/30 (83%)
 Frame = +3

Query: 342 GLINSRYRYLTAETFGFKINANGTSLKKKQ 431
           GLIN   +YLTAE FGFK+NA+ +SLKKKQ
Sbjct: 15  GLINCGNKYLTAEAFGFKVNASASSLKKKQ 44


>UniRef50_UPI0000E5925D Cluster: fascin 2 isoform 2; n=1; Homo
           sapiens|Rep: fascin 2 isoform 2 - Homo sapiens
          Length = 516

 Score = 47.2 bits (107), Expect = 1e-04
 Identities = 20/30 (66%), Positives = 25/30 (83%)
 Frame = +3

Query: 342 GLINSRYRYLTAETFGFKINANGTSLKKKQ 431
           GL+N   RYLTAE+FGFK+NA+  SLK+KQ
Sbjct: 15  GLVNDTDRYLTAESFGFKVNASAPSLKRKQ 44


>UniRef50_Q05634 Cluster: Fascin; n=1; Strongylocentrotus
           purpuratus|Rep: Fascin - Strongylocentrotus purpuratus
           (Purple sea urchin)
          Length = 496

 Score = 44.0 bits (99), Expect = 0.001
 Identities = 19/33 (57%), Positives = 25/33 (75%)
 Frame = +3

Query: 333 WTIGLINSRYRYLTAETFGFKINANGTSLKKKQ 431
           +  GL+NS  RYLTAE FG K+NA+G +LK +Q
Sbjct: 8   YKFGLVNSAGRYLTAEKFGGKVNASGATLKARQ 40


>UniRef50_Q7NI81 Cluster: Glr2302 protein; n=1; Gloeobacter
           violaceus|Rep: Glr2302 protein - Gloeobacter violaceus
          Length = 585

 Score = 32.3 bits (70), Expect = 4.5
 Identities = 13/23 (56%), Positives = 16/23 (69%)
 Frame = -1

Query: 404 SVDLEAEGLGGEVAVPRVYQADG 336
           SVDL A G+ G++ VPR Y  DG
Sbjct: 526 SVDLGANGISGDILVPRDYDGDG 548


>UniRef50_Q1IJA0 Cluster: Type IV pilus assembly PilZ; n=2;
           Acidobacteria bacterium Ellin345|Rep: Type IV pilus
           assembly PilZ - Acidobacteria bacterium (strain
           Ellin345)
          Length = 114

 Score = 31.9 bits (69), Expect = 5.9
 Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 9/53 (16%)
 Frame = +3

Query: 288 NSAGDILTQNQQKGWWTIGLINSRYRYLTAETFG---------FKINANGTSL 419
           N +GD++TQN  + W  +G I++R  YL+  TFG         FKI   GT +
Sbjct: 13  NGSGDLITQNNGRVWGHLGDISTRGFYLS--TFGPWPVNTDVRFKIEVEGTQI 63


>UniRef50_A2EE67 Cluster: Surface antigen BspA-like; n=1;
           Trichomonas vaginalis G3|Rep: Surface antigen BspA-like
           - Trichomonas vaginalis G3
          Length = 993

 Score = 31.5 bits (68), Expect = 7.8
 Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
 Frame = -3

Query: 252 FTLELVYTAEEMQSRDREKTTKCTNYKNIII-IYISLHFSSIRLTLNCRNLS*ITVKKNC 76
           F LE VY++  + + +      CTN K I +   IS+  +S     NC NL  + +  NC
Sbjct: 621 FNLEKVYSSIPVDAVEEYSFYNCTNLKEINLGTVISIKANSFE---NCENLEEVDI-TNC 676

Query: 75  RFILSGEFLKC 43
             I    F KC
Sbjct: 677 ITIGVSAFTKC 687


>UniRef50_Q04893 Cluster: Uncharacterized protein YMR317W; n=1;
           Saccharomyces cerevisiae|Rep: Uncharacterized protein
           YMR317W - Saccharomyces cerevisiae (Baker's yeast)
          Length = 1140

 Score = 31.5 bits (68), Expect = 7.8
 Identities = 15/32 (46%), Positives = 23/32 (71%)
 Frame = +1

Query: 265 TDTAMATSTALETSSPRTSRKDGGPSA**TLG 360
           T+T   +S+A++TSSP+TS  +GG S+   LG
Sbjct: 134 TETDSISSSAIQTSSPQTSSSNGGGSSSEPLG 165


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 380,534,713
Number of Sequences: 1657284
Number of extensions: 6666282
Number of successful extensions: 16109
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 15721
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16105
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 21075479950
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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