BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0002 (431 letters) Database: uniref50 1,657,284 sequences; 575,637,011 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value UniRef50_Q24524 Cluster: Protein singed; n=14; Endopterygota|Rep... 91 7e-18 UniRef50_A7RUD3 Cluster: Predicted protein; n=1; Nematostella ve... 50 2e-05 UniRef50_Q4RZ67 Cluster: Chromosome undetermined SCAF14961, whol... 50 3e-05 UniRef50_Q4RZ66 Cluster: Chromosome undetermined SCAF14961, whol... 50 3e-05 UniRef50_Q6PBS5 Cluster: Fascin homolog 1-like, actin-bundling p... 49 5e-05 UniRef50_Q16658 Cluster: Fascin; n=49; Euteleostomi|Rep: Fascin ... 48 1e-04 UniRef50_UPI0000E5925D Cluster: fascin 2 isoform 2; n=1; Homo sa... 47 1e-04 UniRef50_Q05634 Cluster: Fascin; n=1; Strongylocentrotus purpura... 44 0.001 UniRef50_Q7NI81 Cluster: Glr2302 protein; n=1; Gloeobacter viola... 32 4.5 UniRef50_Q1IJA0 Cluster: Type IV pilus assembly PilZ; n=2; Acido... 32 5.9 UniRef50_A2EE67 Cluster: Surface antigen BspA-like; n=1; Trichom... 31 7.8 UniRef50_Q04893 Cluster: Uncharacterized protein YMR317W; n=1; S... 31 7.8 >UniRef50_Q24524 Cluster: Protein singed; n=14; Endopterygota|Rep: Protein singed - Drosophila melanogaster (Fruit fly) Length = 512 Score = 91.5 bits (217), Expect = 7e-18 Identities = 40/57 (70%), Positives = 48/57 (84%) Frame = +3 Query: 261 MNGHSNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQ 431 MNG +S GDI++QNQQKGWWTIGLIN +++Y+TAETFGFK+NANG SLKKKQ Sbjct: 1 MNGQGCELGHSNGDIISQNQQKGWWTIGLINGQHKYMTAETFGFKLNANGASLKKKQ 57 >UniRef50_A7RUD3 Cluster: Predicted protein; n=1; Nematostella vectensis|Rep: Predicted protein - Nematostella vectensis Length = 488 Score = 50.4 bits (115), Expect = 2e-05 Identities = 22/34 (64%), Positives = 28/34 (82%), Gaps = 1/34 (2%) Frame = +3 Query: 333 WTIGLINSRY-RYLTAETFGFKINANGTSLKKKQ 431 W IGL+N +YLTAETFGFK+N++G SL+KKQ Sbjct: 7 WNIGLMNDNSSKYLTAETFGFKLNSDGVSLRKKQ 40 >UniRef50_Q4RZ67 Cluster: Chromosome undetermined SCAF14961, whole genome shotgun sequence; n=1; Tetraodon nigroviridis|Rep: Chromosome undetermined SCAF14961, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer) Length = 325 Score = 49.6 bits (113), Expect = 3e-05 Identities = 29/53 (54%), Positives = 35/53 (66%) Frame = +3 Query: 273 SNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQ 431 +NG N D+L Q Q GLIN +YLTAETFGFKINA+ +S+KKKQ Sbjct: 3 TNGASNGTSDML-QIQ------FGLINCGNKYLTAETFGFKINASASSMKKKQ 48 >UniRef50_Q4RZ66 Cluster: Chromosome undetermined SCAF14961, whole genome shotgun sequence; n=1; Tetraodon nigroviridis|Rep: Chromosome undetermined SCAF14961, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer) Length = 353 Score = 49.6 bits (113), Expect = 3e-05 Identities = 29/53 (54%), Positives = 35/53 (66%) Frame = +3 Query: 273 SNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQ 431 +NG N D+L Q Q GLIN +YLTAETFGFKINA+ +S+KKKQ Sbjct: 3 TNGASNGTSDML-QIQ------FGLINCGNKYLTAETFGFKINASASSMKKKQ 48 >UniRef50_Q6PBS5 Cluster: Fascin homolog 1-like, actin-bundling protein; n=10; Gnathostomata|Rep: Fascin homolog 1-like, actin-bundling protein - Danio rerio (Zebrafish) (Brachydanio rerio) Length = 290 Score = 48.8 bits (111), Expect = 5e-05 Identities = 22/30 (73%), Positives = 24/30 (80%) Frame = +3 Query: 342 GLINSRYRYLTAETFGFKINANGTSLKKKQ 431 GLIN RYLTAE FGFK+NA+ SLKKKQ Sbjct: 14 GLINHENRYLTAEAFGFKVNASAPSLKKKQ 43 >UniRef50_Q16658 Cluster: Fascin; n=49; Euteleostomi|Rep: Fascin - Homo sapiens (Human) Length = 493 Score = 47.6 bits (108), Expect = 1e-04 Identities = 21/30 (70%), Positives = 25/30 (83%) Frame = +3 Query: 342 GLINSRYRYLTAETFGFKINANGTSLKKKQ 431 GLIN +YLTAE FGFK+NA+ +SLKKKQ Sbjct: 15 GLINCGNKYLTAEAFGFKVNASASSLKKKQ 44 >UniRef50_UPI0000E5925D Cluster: fascin 2 isoform 2; n=1; Homo sapiens|Rep: fascin 2 isoform 2 - Homo sapiens Length = 516 Score = 47.2 bits (107), Expect = 1e-04 Identities = 20/30 (66%), Positives = 25/30 (83%) Frame = +3 Query: 342 GLINSRYRYLTAETFGFKINANGTSLKKKQ 431 GL+N RYLTAE+FGFK+NA+ SLK+KQ Sbjct: 15 GLVNDTDRYLTAESFGFKVNASAPSLKRKQ 44 >UniRef50_Q05634 Cluster: Fascin; n=1; Strongylocentrotus purpuratus|Rep: Fascin - Strongylocentrotus purpuratus (Purple sea urchin) Length = 496 Score = 44.0 bits (99), Expect = 0.001 Identities = 19/33 (57%), Positives = 25/33 (75%) Frame = +3 Query: 333 WTIGLINSRYRYLTAETFGFKINANGTSLKKKQ 431 + GL+NS RYLTAE FG K+NA+G +LK +Q Sbjct: 8 YKFGLVNSAGRYLTAEKFGGKVNASGATLKARQ 40 >UniRef50_Q7NI81 Cluster: Glr2302 protein; n=1; Gloeobacter violaceus|Rep: Glr2302 protein - Gloeobacter violaceus Length = 585 Score = 32.3 bits (70), Expect = 4.5 Identities = 13/23 (56%), Positives = 16/23 (69%) Frame = -1 Query: 404 SVDLEAEGLGGEVAVPRVYQADG 336 SVDL A G+ G++ VPR Y DG Sbjct: 526 SVDLGANGISGDILVPRDYDGDG 548 >UniRef50_Q1IJA0 Cluster: Type IV pilus assembly PilZ; n=2; Acidobacteria bacterium Ellin345|Rep: Type IV pilus assembly PilZ - Acidobacteria bacterium (strain Ellin345) Length = 114 Score = 31.9 bits (69), Expect = 5.9 Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 9/53 (16%) Frame = +3 Query: 288 NSAGDILTQNQQKGWWTIGLINSRYRYLTAETFG---------FKINANGTSL 419 N +GD++TQN + W +G I++R YL+ TFG FKI GT + Sbjct: 13 NGSGDLITQNNGRVWGHLGDISTRGFYLS--TFGPWPVNTDVRFKIEVEGTQI 63 >UniRef50_A2EE67 Cluster: Surface antigen BspA-like; n=1; Trichomonas vaginalis G3|Rep: Surface antigen BspA-like - Trichomonas vaginalis G3 Length = 993 Score = 31.5 bits (68), Expect = 7.8 Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 1/71 (1%) Frame = -3 Query: 252 FTLELVYTAEEMQSRDREKTTKCTNYKNIII-IYISLHFSSIRLTLNCRNLS*ITVKKNC 76 F LE VY++ + + + CTN K I + IS+ +S NC NL + + NC Sbjct: 621 FNLEKVYSSIPVDAVEEYSFYNCTNLKEINLGTVISIKANSFE---NCENLEEVDI-TNC 676 Query: 75 RFILSGEFLKC 43 I F KC Sbjct: 677 ITIGVSAFTKC 687 >UniRef50_Q04893 Cluster: Uncharacterized protein YMR317W; n=1; Saccharomyces cerevisiae|Rep: Uncharacterized protein YMR317W - Saccharomyces cerevisiae (Baker's yeast) Length = 1140 Score = 31.5 bits (68), Expect = 7.8 Identities = 15/32 (46%), Positives = 23/32 (71%) Frame = +1 Query: 265 TDTAMATSTALETSSPRTSRKDGGPSA**TLG 360 T+T +S+A++TSSP+TS +GG S+ LG Sbjct: 134 TETDSISSSAIQTSSPQTSSSNGGGSSSEPLG 165 Database: uniref50 Posted date: Oct 5, 2007 11:19 AM Number of letters in database: 575,637,011 Number of sequences in database: 1,657,284 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 380,534,713 Number of Sequences: 1657284 Number of extensions: 6666282 Number of successful extensions: 16109 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 15721 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 16105 length of database: 575,637,011 effective HSP length: 93 effective length of database: 421,509,599 effective search space used: 21075479950 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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