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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0952
         (822 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g18170.1 68416.m02312 expressed protein contains Pfam domain,...    30   2.1  
At5g67380.1 68418.m08496 casein kinase II alpha chain 1 identica...    29   3.7  
At5g19090.2 68418.m02270 heavy-metal-associated domain-containin...    29   3.7  
At5g19090.1 68418.m02269 heavy-metal-associated domain-containin...    29   3.7  
At3g18180.1 68416.m02313 hypothetical protein contains Pfam doma...    28   6.5  

>At3g18170.1 68416.m02312 expressed protein contains Pfam domain,
           PF04577: Protein of unknown function (DUF563)
          Length = 384

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 19/78 (24%), Positives = 41/78 (52%)
 Frame = +2

Query: 479 SILILVCTFVSLSIPTSHDKSEILLYLLKIVKRFSNHTTTQPNTDVKHAASNKYCSYINI 658
           S+  ++ +    S+   HD ++I++ L    +RF        N +V+   +NK  S+IN 
Sbjct: 87  SVPAMIFSLGGYSMNNFHDFTDIVIPLYTTARRF--------NGEVQFLVTNKSPSWINK 138

Query: 659 QKNMILKLFWFTILHIEK 712
            K ++ KL  + +++I++
Sbjct: 139 FKELVRKLSNYEVIYIDE 156


>At5g67380.1 68418.m08496 casein kinase II alpha chain 1 identical
           to casein kinase II, alpha chain 1 (CK II) [Arabidopsis
           thaliana] SWISS-PROT:Q08467; contains protein kinase
           domain, Pfam:PF00069
          Length = 409

 Score = 29.1 bits (62), Expect = 3.7
 Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 3/54 (5%)
 Frame = +2

Query: 485 LILVCTFVSLSIPTSHD---KSEILLYLLKIVKRFSNHTTTQPNTDVKHAASNK 637
           L+L+C  ++L  PT+H    +S I+    + V   S  TT  P+  V+ + SN+
Sbjct: 20  LLLLCAVLALRAPTAHSPILRSSIVTPTARAVSEVSGCTTIDPDFLVEISDSNQ 73


>At5g19090.2 68418.m02270 heavy-metal-associated domain-containing
           protein contains Pfam heavy-metal-associated domain
           PF00403; glycine-rich protein GRP22, rape, PIR:S31415;
           isoform contains a non-consensus TG-acceptor splice site
           at intron 3
          Length = 465

 Score = 29.1 bits (62), Expect = 3.7
 Identities = 18/56 (32%), Positives = 27/56 (48%)
 Frame = +1

Query: 262 GGAQGVGEVPPGGINALLEVGQSLAQQMQAANPDLVEQLRRQLRPPGSGENQPPSQ 429
           GG  G G    GG    + + Q   QQMQ  NP  ++QL++  +  G  + + P Q
Sbjct: 126 GGGGGGGGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDLKLPPQ 181


>At5g19090.1 68418.m02269 heavy-metal-associated domain-containing
           protein contains Pfam heavy-metal-associated domain
           PF00403; glycine-rich protein GRP22, rape, PIR:S31415;
           isoform contains a non-consensus TG-acceptor splice site
           at intron 3
          Length = 587

 Score = 29.1 bits (62), Expect = 3.7
 Identities = 18/56 (32%), Positives = 27/56 (48%)
 Frame = +1

Query: 262 GGAQGVGEVPPGGINALLEVGQSLAQQMQAANPDLVEQLRRQLRPPGSGENQPPSQ 429
           GG  G G    GG    + + Q   QQMQ  NP  ++QL++  +  G  + + P Q
Sbjct: 126 GGGGGGGGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDLKLPPQ 181


>At3g18180.1 68416.m02313 hypothetical protein contains Pfam domain,
           PF04577: Protein of unknown function (DUF563)
          Length = 470

 Score = 28.3 bits (60), Expect = 6.5
 Identities = 18/78 (23%), Positives = 40/78 (51%)
 Frame = +2

Query: 479 SILILVCTFVSLSIPTSHDKSEILLYLLKIVKRFSNHTTTQPNTDVKHAASNKYCSYINI 658
           S+  ++ +    S+   HD ++I++ L    +RFS         +V+   +NK   +IN 
Sbjct: 172 SVPAILFSLGGFSLNNFHDFTDIVIPLYTTARRFSG--------EVQFLVTNKNLLWINK 223

Query: 659 QKNMILKLFWFTILHIEK 712
            K ++ KL  + +++I++
Sbjct: 224 FKELVRKLSNYEVIYIDE 241


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,904,433
Number of Sequences: 28952
Number of extensions: 362034
Number of successful extensions: 775
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 748
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 775
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1882599200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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