BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0948 (834 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_50623| Best HMM Match : NAD_Gly3P_dh_C (HMM E-Value=0) 72 7e-13 SB_49139| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 3e-06 SB_22860| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.0 SB_37159| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.6 SB_6904| Best HMM Match : NifT (HMM E-Value=8.7) 29 4.6 >SB_50623| Best HMM Match : NAD_Gly3P_dh_C (HMM E-Value=0) Length = 358 Score = 71.7 bits (168), Expect = 7e-13 Identities = 40/90 (44%), Positives = 56/90 (62%), Gaps = 5/90 (5%) Frame = +2 Query: 251 EVTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQ---FVRTICSTL 421 ++TEIIN H NVKYLP +P N+ A PDVVE KDAD+L+FVVPHQ +T + + Sbjct: 53 KLTEIINTKHMNVKYLPDFLIPENIHANPDVVECVKDADILVFVVPHQGLDHTKTGLTLV 112 Query: 422 LGKIKPT--AAALSLIKGFDIAEVVASILY 505 IK +S++ G ++A+ VA L+ Sbjct: 113 SNVIKEALDLPEVSVLMGANLAKEVARELF 142 Score = 52.4 bits (120), Expect = 4e-07 Identities = 22/49 (44%), Positives = 36/49 (73%), Gaps = 1/49 (2%) Frame = +3 Query: 105 KQPKNKVCIVGSGNWGSAIAKIVGRNAASLSN-FEDRVTMWVYEEIIEG 248 ++ + +V I+GSGNWGS IA+IVG+N ++ F + V M+ YEE+++G Sbjct: 3 EEGRKRVAIIGSGNWGSVIARIVGQNVKEHNDVFFEGVEMYTYEELVDG 51 Score = 38.3 bits (85), Expect = 0.008 Identities = 15/38 (39%), Positives = 24/38 (63%) Frame = +1 Query: 631 PLMRDIIQTDYFRVRGGGP*XRIEICGALKTIVAVGSG 744 PL++ + T YF++ +E+CGALK VA+G+G Sbjct: 216 PLLKQLFHTHYFKINVVEDADTVELCGALKNAVAIGAG 253 Score = 36.7 bits (81), Expect = 0.023 Identities = 19/34 (55%), Positives = 23/34 (67%), Gaps = 1/34 (2%) Frame = +3 Query: 510 IITRCLKIP-CAVLMGANIASEVAEEKFCETTIG 608 +I L +P +VLMGAN+A EVA E F E TIG Sbjct: 115 VIKEALDLPEVSVLMGANLAKEVARELFGEATIG 148 >SB_49139| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 772 Score = 49.6 bits (113), Expect = 3e-06 Identities = 21/44 (47%), Positives = 32/44 (72%), Gaps = 1/44 (2%) Frame = +3 Query: 120 KVCIVGSGNWGSAIAKIVGRNAASLSN-FEDRVTMWVYEEIIEG 248 KV ++GSGNWG+AIA+I+G N + F ++V M+VY+ +I G Sbjct: 229 KVTVLGSGNWGTAIARIIGDNVRKKPHLFHNKVQMYVYDSLING 272 Score = 43.2 bits (97), Expect = 3e-04 Identities = 28/76 (36%), Positives = 38/76 (50%) Frame = +2 Query: 248 KEVTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLG 427 ++++EIIN HENVK LPG K+P NV F+ ++C + Sbjct: 273 RKLSEIINTEHENVKDLPGFKIPPNV-----------------------FLDSVCQKIKS 309 Query: 428 KIKPTAAALSLIKGFD 475 IKP A+SLIKG D Sbjct: 310 SIKPDVLAISLIKGLD 325 Score = 35.1 bits (77), Expect = 0.071 Identities = 16/25 (64%), Positives = 20/25 (80%) Frame = +3 Query: 540 AVLMGANIASEVAEEKFCETTIGCR 614 +V+MGAN+A EVA+ F ETTIG R Sbjct: 348 SVMMGANLADEVAKGFFSETTIGSR 372 >SB_22860| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 682 Score = 30.3 bits (65), Expect = 2.0 Identities = 14/29 (48%), Positives = 15/29 (51%) Frame = -1 Query: 810 GPESG*PAAFCGNPP*GGQSRKPGTHCHN 724 G ES P C NP G +S PG CHN Sbjct: 252 GNESNGPGKECHNP--GNESNDPGKECHN 278 Score = 29.1 bits (62), Expect = 4.6 Identities = 15/33 (45%), Positives = 17/33 (51%) Frame = -1 Query: 822 HLPWGPESG*PAAFCGNPP*GGQSRKPGTHCHN 724 H P G +S P C NP G +S PG CHN Sbjct: 277 HNP-GNKSNDPGKECHNP--GNESNDPGKECHN 306 Score = 29.1 bits (62), Expect = 4.6 Identities = 15/33 (45%), Positives = 17/33 (51%) Frame = -1 Query: 822 HLPWGPESG*PAAFCGNPP*GGQSRKPGTHCHN 724 H P G +S P C NP G +S PG CHN Sbjct: 305 HNP-GNKSNDPGKKCHNP--GNESNDPGKECHN 334 Score = 28.7 bits (61), Expect = 6.1 Identities = 15/33 (45%), Positives = 17/33 (51%) Frame = -1 Query: 822 HLPWGPESG*PAAFCGNPP*GGQSRKPGTHCHN 724 H P G ES P C NP G +S PG CH+ Sbjct: 347 HNP-GNESNDPGKECHNP--GNESNDPGKECHH 376 >SB_37159| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 450 Score = 29.1 bits (62), Expect = 4.6 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = -1 Query: 609 NQWSFRRIFPQQPPMQYWLPLIQHREFLGIL 517 NQ F + P P +YW P+I R F +L Sbjct: 408 NQRPFTLVVPDLQPRKYWWPIINSRAFETLL 438 >SB_6904| Best HMM Match : NifT (HMM E-Value=8.7) Length = 408 Score = 29.1 bits (62), Expect = 4.6 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = -1 Query: 609 NQWSFRRIFPQQPPMQYWLPLIQHREFLGIL 517 NQ F + P P +YW P+I R F +L Sbjct: 302 NQRPFTIVIPNLQPRRYWWPIINSRAFETLL 332 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 25,936,153 Number of Sequences: 59808 Number of extensions: 548000 Number of successful extensions: 1356 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 1242 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1350 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2347493764 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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