Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0948
(834 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD... 91 6e-19
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy... 65 6e-11
At5g63910.1 68418.m08025 expressed protein 31 1.2
At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transfera... 29 2.9
At5g27690.1 68418.m03321 heavy-metal-associated domain-containin... 29 3.8
At2g29770.1 68415.m03617 kelch repeat-containing F-box family pr... 29 3.8
At5g57190.1 68418.m07144 phosphatidylserine decarboxylase, putat... 29 5.0
At4g25970.1 68417.m03737 phosphatidylserine decarboxylase, putat... 29 5.0
At5g19970.1 68418.m02377 expressed protein ; expression support... 28 6.6
At2g38860.2 68415.m04775 proteaseI (pfpI)-like protein (YLS5) co... 28 6.6
At2g38860.1 68415.m04774 proteaseI (pfpI)-like protein (YLS5) co... 28 6.6
>At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+]
/ GPDH strong similarity to SP|P52425
Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)
{Cuphea lanceolata}; contains Pfam profile PF01210:
NAD-dependent glycerol-3-phosphate dehydrogenase
Length = 400
Score = 91.5 bits (217), Expect = 6e-19
Identities = 44/103 (42%), Positives = 66/103 (64%)
Frame = +2
Query: 248 KEVTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLG 427
+++ ++IN+T+ENVKYLPG KL NVVA PD+ A KDA++L+FV PHQF+ IC L G
Sbjct: 100 EKLNDVINKTNENVKYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDG 159
Query: 428 KIKPTAAALSLIKGFDIAEVVASILYHYYYKMPKNSLCCINGS 556
KI A+SL+KG ++ + ++ K + C + G+
Sbjct: 160 KITGDVEAISLVKGMEVKKEGPCMISSLISKQLGINCCVLMGA 202
Score = 61.3 bits (142), Expect = 8e-10
Identities = 24/43 (55%), Positives = 32/43 (74%)
Frame = +3
Query: 114 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEII 242
K+KV +VGSGNWGS AK++ NA L +F D V MWV+EE++
Sbjct: 54 KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVL 96
Score = 46.4 bits (105), Expect = 2e-05
Identities = 21/36 (58%), Positives = 28/36 (77%)
Frame = +3
Query: 507 TIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCR 614
++I++ L I C VLMGANIA+E+A EKF E T+G R
Sbjct: 186 SLISKQLGINCCVLMGANIANEIAVEKFSEATVGYR 221
Score = 31.1 bits (67), Expect = 0.94
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +1
Query: 646 IIQTDYFRVRGGGP*XRIEICGALKTIVAVGSGF 747
+ T YF V +E+CG LK +VA+ +GF
Sbjct: 233 LFSTPYFMVTPVHDVEGVELCGTLKNVVAIAAGF 266
>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein low similarity to SP|Q26756
Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
(EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 420
Score = 64.9 bits (151), Expect = 6e-11
Identities = 34/106 (32%), Positives = 51/106 (48%), Gaps = 1/106 (0%)
Frame = +2
Query: 254 VTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKI 433
V + INE H N KY P HKLP NV+A D A DAD + VP QF + + +
Sbjct: 124 VCQSINENHHNCKYFPEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYV 183
Query: 434 KPTAAALSLIKGFDIAEV-VASILYHYYYKMPKNSLCCINGSQYCI 568
P +SL KG ++ + + S + K P+ ++G + +
Sbjct: 184 DPGLPFISLSKGLELNTLRMMSQIIPIALKNPRQPFVALSGPSFAL 229
Score = 29.1 bits (62), Expect = 3.8
Identities = 15/59 (25%), Positives = 30/59 (50%), Gaps = 5/59 (8%)
Frame = +1
Query: 628 APLMRDIIQTDYFRVRGGGP*XRIEICGALKTIVAVGSGFP-----GLASLRWITTKGC 789
A ++ ++ + Y R+ +EI GALK ++A+ +G G S+ + ++GC
Sbjct: 249 ANAVQQLLASSYLRINTSSDVTGVEIAGALKNVLAIAAGIVDGMNLGNNSMAALVSQGC 307
>At5g63910.1 68418.m08025 expressed protein
Length = 500
Score = 30.7 bits (66), Expect = 1.2
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = +3
Query: 123 VCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEG 248
VCIVGSG GS++A + + S + ++ M+ EI+ G
Sbjct: 38 VCIVGSGIGGSSVAHFLRNYSVSTGLNQAKILMFERHEIVGG 79
>At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 487
Score = 29.5 bits (63), Expect = 2.9
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = +3
Query: 87 ILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEG 248
+LD +KQP V + G+ GS AK + A L + R +WV ++G
Sbjct: 258 VLDWLNKQPDESVLYISFGSGGSLSAKQLTELAWGLEMSQQRF-VWVVRPPVDG 310
>At5g27690.1 68418.m03321 heavy-metal-associated domain-containing
protein very low similarity to copper homeostasis factor
from Arabidopsis thaliana [gi:3168840]; contains Pfam
heavy metal associated domain PF00403
Length = 352
Score = 29.1 bits (62), Expect = 3.8
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -2
Query: 752 PGNPEPTATMVFNAPHISMRXHGPPP 675
P N P T++++AP HGPPP
Sbjct: 204 PQNDGPPETVIYSAPQDHHIIHGPPP 229
>At2g29770.1 68415.m03617 kelch repeat-containing F-box family
protein contains Pfam PF00646: F-box domain; contains
Pfam PF01344 : Kelch motif; similar to SKP1 interacting
partner 6 (GI:10716957) [Arabidopsis thaliana]
Length = 387
Score = 29.1 bits (62), Expect = 3.8
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2
Query: 545 INGSQYCIGGC*GKILRNDHWL 610
I+G Y IGGC + RNDHW+
Sbjct: 181 IDGRIYVIGGC---VKRNDHWI 199
>At5g57190.1 68418.m07144 phosphatidylserine decarboxylase, putative
similar to SP|P53037 Phosphatidylserine decarboxylase
proenzyme 2 precursor (EC 4.1.1.65) {Saccharomyces
cerevisiae}; contains Pfam profile PF02666:
phosphatidylserine decarboxylase
Length = 615
Score = 28.7 bits (61), Expect = 5.0
Identities = 22/96 (22%), Positives = 42/96 (43%), Gaps = 1/96 (1%)
Frame = +2
Query: 332 VPDVVEAAKDADLLIFV-VPHQFVRTICSTLLGKIKPTAAALSLIKGFDIAEVVASILYH 508
+P +E KD + V P Q +T + ++KP A ++ + D+A A
Sbjct: 368 IPRFLEFFKDQINMAEVKYPLQHFKTFNEFFIRELKPGARPIACMNRNDVAVCAADCRLM 427
Query: 509 YYYKMPKNSLCCINGSQYCIGGC*GKILRNDHWLSG 616
+ + ++ I G ++ I G GK + + +L G
Sbjct: 428 AFQSVEDSTRFWIKGKKFSIRGLLGKNVNPNAFLDG 463
>At4g25970.1 68417.m03737 phosphatidylserine decarboxylase, putative
similar to SP|P53037 Phosphatidylserine decarboxylase
proenzyme 2 precursor (EC 4.1.1.65) {Saccharomyces
cerevisiae}; contains Pfam profile PF02666:
phosphatidylserine decarboxylase
Length = 635
Score = 28.7 bits (61), Expect = 5.0
Identities = 27/118 (22%), Positives = 51/118 (43%), Gaps = 3/118 (2%)
Frame = +2
Query: 272 ETHENVKYLPGHKLPSNVVA--VPDVVEAAKDADLLIFV-VPHQFVRTICSTLLGKIKPT 442
E +N+ G K+ S A +P +E KD + V P +T + ++KP
Sbjct: 365 EILQNLSEKQGKKMNSVESAQNIPSFLEFFKDQINMAEVKYPLDHFKTFNEFFVRELKPG 424
Query: 443 AAALSLIKGFDIAEVVASILYHYYYKMPKNSLCCINGSQYCIGGC*GKILRNDHWLSG 616
A ++ + D+A A + + ++ I G ++ I G G +++D +L G
Sbjct: 425 ARPIACMDQDDVAVSAADCRLMAFQSVDDSTRFWIKGRKFSIKGLLGNDVQSDAFLDG 482
>At5g19970.1 68418.m02377 expressed protein ; expression supported
by MPSS
Length = 363
Score = 28.3 bits (60), Expect = 6.6
Identities = 15/51 (29%), Positives = 23/51 (45%), Gaps = 3/51 (5%)
Frame = +3
Query: 567 SEVAEEKFCETTIGCRDVMLGSVNAGYHTDRLLQGPW---WWTMXTHRNMW 710
SEV +K C +GC + LG + T+ +G W W H++ W
Sbjct: 286 SEVISKKPCGVMMGCLERRLGVIRVALMTNH--EGLWNIIWLDYDKHKDKW 334
>At2g38860.2 68415.m04775 proteaseI (pfpI)-like protein (YLS5)
contains Pfam profile PF01965: DJ-1/PfpI family;
supporting cDNA gi|13122287|dbj|AB047808.1|; identical
to proteaseI (pfpI)-like protein [Arabidopsis thaliana]
GI:13122288, cDNA proteaseI (pfpI)-like protein
GI:13122287
Length = 398
Score = 28.3 bits (60), Expect = 6.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +3
Query: 663 LQGPWWWTMXTHRNMWSVKDHCGSGFRVSWIGLPTVDYHKRLLV 794
L G WW ++M+ + D G +S +G PT+ + +LL+
Sbjct: 152 LSGGEWWQQPGIQSMFEITDCVKDGNFMSTVGWPTLGHGIKLLL 195
>At2g38860.1 68415.m04774 proteaseI (pfpI)-like protein (YLS5)
contains Pfam profile PF01965: DJ-1/PfpI family;
supporting cDNA gi|13122287|dbj|AB047808.1|; identical
to proteaseI (pfpI)-like protein [Arabidopsis thaliana]
GI:13122288, cDNA proteaseI (pfpI)-like protein
GI:13122287
Length = 389
Score = 28.3 bits (60), Expect = 6.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +3
Query: 663 LQGPWWWTMXTHRNMWSVKDHCGSGFRVSWIGLPTVDYHKRLLV 794
L G WW ++M+ + D G +S +G PT+ + +LL+
Sbjct: 143 LSGGEWWQQPGIQSMFEITDCVKDGNFMSTVGWPTLGHGIKLLL 186
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,255,125
Number of Sequences: 28952
Number of extensions: 391601
Number of successful extensions: 1092
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1035
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1092
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1921616800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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