Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0938
(832 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 58 9e-09
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 51 1e-06
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 48 8e-06
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 47 2e-05
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 44 1e-04
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 43 3e-04
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 41 0.001
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 41 0.001
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 40 0.002
At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4) ... 30 2.2
At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subuni... 30 2.2
At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subuni... 30 2.2
At3g16890.1 68416.m02159 pentatricopeptide (PPR) repeat-containi... 29 2.9
At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) ... 29 3.8
At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 29 5.0
At5g39080.1 68418.m04728 transferase family protein similar to a... 28 6.6
At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative... 28 6.6
At5g49650.2 68418.m06145 xylulose kinase, putative similar to D-... 28 8.7
At5g49650.1 68418.m06146 xylulose kinase, putative similar to D-... 28 8.7
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 57.6 bits (133), Expect = 9e-09
Identities = 34/104 (32%), Positives = 45/104 (43%)
Frame = +3
Query: 261 FILHVGGVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILL 440
F +H + + F + A+W T +F Y GLGIT HR AH+S+K L LL
Sbjct: 48 FTVHSLALLAPFYFTWSALWVTFLF----YTIGGLGITVSYHRNLAHRSFKVPKWLEYLL 103
Query: 441 TIFNTIAFQDAVVDWARDHRMHQNIQRPMRTPTTQPEDSSFPTL 572
+A Q +DW HR H R P + E F L
Sbjct: 104 AYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHL 147
Score = 38.3 bits (85), Expect = 0.006
Identities = 17/55 (30%), Positives = 31/55 (56%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 673
++++++ DPH+ GF+FSH+ W+ + K G +V DL+ RF +K
Sbjct: 127 QFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC-GRRANVEDLKRQWFYRFLQK 180
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 50.8 bits (116), Expect = 1e-06
Identities = 28/89 (31%), Positives = 35/89 (39%)
Frame = +3
Query: 306 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 485
F W A+ +Y GLGIT HR AH+S+K L +A Q +DW
Sbjct: 57 FNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDW 116
Query: 486 ARDHRMHQNIQRPMRTPTTQPEDSSFPTL 572
HR H R P + E F L
Sbjct: 117 VSTHRYHHQFTDSDRDPHSPNEGFWFSHL 145
Score = 38.3 bits (85), Expect = 0.006
Identities = 19/62 (30%), Positives = 35/62 (56%), Gaps = 2/62 (3%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK--YYQ 682
+++++D DPH+ GF+FSH+ WL + ++ G +V DL+ +F ++ Y
Sbjct: 125 QFTDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVLYH 183
Query: 683 IL 688
IL
Sbjct: 184 IL 185
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 48.0 bits (109), Expect = 8e-06
Identities = 29/93 (31%), Positives = 35/93 (37%)
Frame = +3
Query: 306 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 485
F W F LY + L IT HR AH+S+K L L F A Q +DW
Sbjct: 51 FNYKWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDW 110
Query: 486 ARDHRMHQNIQRPMRTPTTQPEDSSFPTLAGCC 584
HR H R P + E F + C
Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWIC 143
Score = 38.7 bits (86), Expect = 0.005
Identities = 15/45 (33%), Positives = 29/45 (64%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLR 643
+++++D DPH+ GF+FSH+ W+ ++ + K G +V DL+
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRN-NVMDLK 162
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 46.8 bits (106), Expect = 2e-05
Identities = 27/78 (34%), Positives = 34/78 (43%), Gaps = 1/78 (1%)
Frame = +3
Query: 306 FKAMWRTSIFAIFLYLCSGL-GITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVD 482
F+ WR A LY+ +GL GIT HR +HK++K L L A Q +D
Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180
Query: 483 WARDHRMHQNIQRPMRTP 536
W HR H R P
Sbjct: 181 WVSTHRYHHQFCDSDRDP 198
Score = 44.8 bits (101), Expect = 7e-05
Identities = 23/64 (35%), Positives = 35/64 (54%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 688
++ ++D DPH+ GF+FSH+ W + + + G +V DL P RF + Y IL
Sbjct: 190 QFCDSDRDPHSPLDGFWFSHMNW-MFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTY-IL 247
Query: 689 MPLA 700
PLA
Sbjct: 248 HPLA 251
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 44.0 bits (99), Expect = 1e-04
Identities = 24/72 (33%), Positives = 38/72 (52%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 688
K+ ET DPH+ T+GF+FSH+ W + ++ G +V+DL P RF ++ +L
Sbjct: 113 KHCETQRDPHSPTQGFWFSHMAW-IFDTSSILENCGGEENVDDLVRQPFYRFLQRTV-LL 170
Query: 689 MPLAXFHHAYIC 724
+A Y C
Sbjct: 171 HMMAYSFLFYFC 182
Score = 41.5 bits (93), Expect = 7e-04
Identities = 25/96 (26%), Positives = 42/96 (43%)
Frame = +3
Query: 288 GGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQ 467
G L +A W +F +++ +G+ IT HR +H+S+ L L +AFQ
Sbjct: 43 GSILLQLEAFW---VFPWLVFI-NGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQ 98
Query: 468 DAVVDWARDHRMHQNIQRPMRTPTTQPEDSSFPTLA 575
++W +HR H R P + + F +A
Sbjct: 99 GDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 134
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 42.7 bits (96), Expect = 3e-04
Identities = 23/77 (29%), Positives = 28/77 (36%)
Frame = +3
Query: 306 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 485
F W F + L + + L IT HR HKS+K L A Q +DW
Sbjct: 51 FNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 486 ARDHRMHQNIQRPMRTP 536
HR H R P
Sbjct: 111 VSTHRFHHQFTDSDRDP 127
Score = 38.7 bits (86), Expect = 0.005
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 664
+++++D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 40.7 bits (91), Expect = 0.001
Identities = 27/98 (27%), Positives = 37/98 (37%), Gaps = 1/98 (1%)
Frame = +3
Query: 246 KCDLFFILHVGGVYGGYLFL-FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARL 422
+ D+ + VG V+ L F W FA + + + L IT HR H+S+K
Sbjct: 30 RVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPK 89
Query: 423 PLRILLTIFNTIAFQDAVVDWARDHRMHQNIQRPMRTP 536
L A Q +DW HR H R P
Sbjct: 90 WLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDP 127
Score = 38.7 bits (86), Expect = 0.005
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 664
+++++D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 40.7 bits (91), Expect = 0.001
Identities = 19/55 (34%), Positives = 31/55 (56%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 673
+++++D DPH+ GF+FSH+ W+ + K G +V DL+ RF KK
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREKC-GRRNNVMDLKQQWFYRFLKK 172
Score = 39.9 bits (89), Expect = 0.002
Identities = 23/77 (29%), Positives = 29/77 (37%)
Frame = +3
Query: 306 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 485
F W F I L + + L IT HR H+S+K L +A Q +DW
Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110
Query: 486 ARDHRMHQNIQRPMRTP 536
HR H R P
Sbjct: 111 VSIHRFHHQFTDSDRDP 127
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 40.3 bits (90), Expect = 0.002
Identities = 24/86 (27%), Positives = 32/86 (37%)
Frame = +3
Query: 306 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 485
F W F + L+ + L IT HR +H+S+K L A Q +DW
Sbjct: 52 FNFKWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDW 111
Query: 486 ARDHRMHQNIQRPMRTPTTQPEDSSF 563
HR H R P + E F
Sbjct: 112 VSIHRFHHQFTDSDRDPHSPKEGLLF 137
Score = 36.7 bits (81), Expect = 0.019
Identities = 16/55 (29%), Positives = 31/55 (56%)
Frame = +2
Query: 509 KYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 673
+++++D DPH+ G FSHI W+ ++ + K G +V DL+ +F ++
Sbjct: 120 QFTDSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRD-NVLDLKKQWFYKFLRR 173
>At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4)
identical to metal transporter Nramp4 [Arabidopsis
thaliana] gi|6468014|gb|AAF13279; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 512
Score = 29.9 bits (64), Expect = 2.2
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +3
Query: 279 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 386
G GY ++ MW T+I + L + LG+ G H
Sbjct: 79 GAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRH 114
>At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 643
Score = 29.9 bits (64), Expect = 2.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +1
Query: 202 VPPSAEKRPWKIVWRNVICSLFCMSVVFMEGTCSFSKPCGG 324
VP S WK + + + LFC S+ F+E + S++ P G
Sbjct: 122 VPQSQVDTSWKNL-THALSGLFCASINFLESSTSYAAPTWG 161
>At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 644
Score = 29.9 bits (64), Expect = 2.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +1
Query: 202 VPPSAEKRPWKIVWRNVICSLFCMSVVFMEGTCSFSKPCGG 324
VP S WK + + + LFC S+ F+E + S++ P G
Sbjct: 122 VPQSQVDTSWKNL-THALSGLFCASINFLESSTSYAAPTWG 161
>At3g16890.1 68416.m02159 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 659
Score = 29.5 bits (63), Expect = 2.9
Identities = 22/81 (27%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
Frame = +3
Query: 99 VHCFSENASPKETGGFVGIVRSRRQ*P-ARRCTAPCTSLSREETLEDCVEKCDLFFILHV 275
++C S N+ KETG F+ + R P + A + L + L + D F V
Sbjct: 327 LYCLSNNSMAKETGQFLRKIGERGYIPDSSTFNAAMSCLLKGHDLVETCRIFDGFVSRGV 386
Query: 276 GGVYGGYLFLFKAMWRTSIFA 338
+ GYL L +A+ F+
Sbjct: 387 KPGFNGYLVLVQALLNAQRFS 407
>At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3)
identical to metal transporter Nramp3 [Arabidopsis
thaliana] gi|6468012|gb|AAF13278; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 509
Score = 29.1 bits (62), Expect = 3.8
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +3
Query: 279 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 386
G GY L+ MW T++ + L + LG+ G H
Sbjct: 83 GAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRH 118
>At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:7381227;
contains PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 468
Score = 28.7 bits (61), Expect = 5.0
Identities = 16/58 (27%), Positives = 24/58 (41%)
Frame = +1
Query: 145 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWRNVICSLFCMSVVFMEGTCSFSKPC 318
S+ ++ D +P D P P S KR W +R +L V+ G + PC
Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPC 181
>At5g39080.1 68418.m04728 transferase family protein similar to
anthocyanin 5-aromatic acyltransferase from Gentiana
triflora GI:4185599, malonyl CoA:anthocyanin
5-O-glucoside-6'''-O-malonyltransferase from Perilla
frutescens GI:17980232, Salvia splendens GI:17980234;
contains Pfam profile PF02458 transferase family
Length = 463
Score = 28.3 bits (60), Expect = 6.6
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = +1
Query: 655 PTFSEEVLPNFNAFSLXSSCLHMYQHCGGETVWNPFN 765
P F ++PN SL SSCL Y G+ +W P +
Sbjct: 55 PFFDSVIVPNLKT-SL-SSCLSHYLPLAGKLIWEPLD 89
>At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative
(NRAMP2) similar to metal transporter Nramp3
[Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of
the natural resistance-associated macrophage protein
(NRAMP) metal transporter family, PMID:11500563
Length = 530
Score = 28.3 bits (60), Expect = 6.6
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +3
Query: 279 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 386
G GY L+ MW T++ + L + +G+ G H
Sbjct: 95 GAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRH 130
>At5g49650.2 68418.m06145 xylulose kinase, putative similar to
D-xylulokinase [Pichia stipitis] gi|8100400|gb|AAF72328
Length = 426
Score = 27.9 bits (59), Expect = 8.7
Identities = 16/46 (34%), Positives = 20/46 (43%)
Frame = +2
Query: 644 NDPILRFQKKYYQILMPLAXFHHAYICTNIVGEKQFGILSTSCGKF 781
N+ L F +IL PL H YI N GE G+ G+F
Sbjct: 373 NNGKLGFYYTENEILPPLPVGSHRYILENFSGESLEGVKEQEVGEF 418
>At5g49650.1 68418.m06146 xylulose kinase, putative similar to
D-xylulokinase [Pichia stipitis] gi|8100400|gb|AAF72328
Length = 558
Score = 27.9 bits (59), Expect = 8.7
Identities = 16/46 (34%), Positives = 20/46 (43%)
Frame = +2
Query: 644 NDPILRFQKKYYQILMPLAXFHHAYICTNIVGEKQFGILSTSCGKF 781
N+ L F +IL PL H YI N GE G+ G+F
Sbjct: 373 NNGKLGFYYTENEILPPLPVGSHRYILENFSGESLEGVKEQEVGEF 418
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,601,942
Number of Sequences: 28952
Number of extensions: 498479
Number of successful extensions: 1351
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1289
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1350
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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