Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0916
(832 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g08335.1 68418.m00981 isoprenylcysteine carboxyl methyltransf... 32 0.54
At2g26450.1 68415.m03173 pectinesterase family protein contains ... 31 0.94
At1g61950.1 68414.m06988 calcium-dependent protein kinase, putat... 31 0.94
At4g03490.1 68417.m00476 ankyrin repeat family protein contains ... 30 2.2
At2g01950.1 68415.m00130 leucine-rich repeat transmembrane prote... 30 2.2
At1g11210.1 68414.m01284 expressed protein contains similarity t... 29 3.8
At4g04720.1 68417.m00693 calcium-dependent protein kinase, putat... 29 5.0
At2g34830.1 68415.m04276 WRKY family transcription factor 28 6.6
At5g19360.1 68418.m02307 calcium-dependent protein kinase, putat... 28 8.7
At4g21940.1 68417.m03174 calcium-dependent protein kinase, putat... 28 8.7
>At5g08335.1 68418.m00981 isoprenylcysteine carboxyl
methyltransferase family protein / ICMT family protein
similar to SP|O60725 Protein-S isoprenylcysteine
O-methyltransferase (EC 2.1.1.100) (Isoprenylcysteine
carboxylmethyltransferase) {Homo sapiens}; contains Pfam
profile PF04140: Isoprenylcysteine carboxyl
methyltransferase (ICMT) family
Length = 197
Score = 31.9 bits (69), Expect = 0.54
Identities = 25/82 (30%), Positives = 38/82 (46%), Gaps = 1/82 (1%)
Frame = +3
Query: 132 IPXGRS-PHVVPVVRAERLHGHVLGLRQIPRHNVCDSKFIWSLGGCVMVLGRLKVDIACG 308
I GRS H++ + R E G+ QI RH IWS+G VM+ +
Sbjct: 98 ITAGRSFTHLIKIRREEHHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNPIS-----A 152
Query: 309 VLFFIVVLQTFAYSILWDVQFL 374
+ F +VV + FA I ++ +L
Sbjct: 153 IAFAVVVWRFFAERIPYEEHYL 174
>At2g26450.1 68415.m03173 pectinesterase family protein contains
Pfam profiles: PF01095 pectinesterase,PF04043 plant
invertase/pectin methylesterase inhibitor
Length = 614
Score = 31.1 bits (67), Expect = 0.94
Identities = 23/77 (29%), Positives = 35/77 (45%)
Frame = -1
Query: 472 SPSDGTPANRLRPSARASANTSS*PPLNAKLRRRNCTSHSILYANVCNTTMKNSTPQAIS 293
SPS TP+ + SA S P K+ + C+S LY +C T+KN T + +
Sbjct: 82 SPSPPTPSQKAPVSAAQSVK----PGQGDKIIQTLCSS--TLYMQICEKTLKNRTDKGFA 135
Query: 292 TLSLPSTITHPPNDQIN 242
L P+T + +N
Sbjct: 136 -LDNPTTFLKSAIEAVN 151
>At1g61950.1 68414.m06988 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase GI:3283996 from [Nicotiana tabacum]; contains
protein kinase domain, Pfam:PF00069; contains EF hand
domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 551
Score = 31.1 bits (67), Expect = 0.94
Identities = 20/68 (29%), Positives = 33/68 (48%)
Frame = +2
Query: 287 QSGYRLRRAVLHSCITDIRVQYTMGRTVPSPQFGIERRLTAGVGGGPS*RAQSVRGRAVA 466
QS + +L DI+ +Y++GR + QFGI T + G + +S+ R +
Sbjct: 77 QSTLQQPEPILGRPFEDIKEKYSLGRELGRGQFGITYICTE-ISSGKNFACKSILKRKLI 135
Query: 467 RREQTEDV 490
R + EDV
Sbjct: 136 RTKDREDV 143
>At4g03490.1 68417.m00476 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 587
Score = 29.9 bits (64), Expect = 2.2
Identities = 27/89 (30%), Positives = 39/89 (43%), Gaps = 2/89 (2%)
Frame = +2
Query: 335 DIRVQYTMGRTVPSPQFGIERRLTAGVGGGPS*RAQSVRGRAVARREQTEDVLGNSPVHP 514
DI VQ+ + R S Q G A G +R V R +D+ GN+P+H
Sbjct: 244 DIGVQHMLTRFASSTQ-GQNVLHVAAKSGNARAVGYLLRKSDVKRLINEQDIEGNTPLHL 302
Query: 515 AGLHVHHALKI--*NLIPADHTRSSREHL 595
A + H + + L+ A TR+ R HL
Sbjct: 303 ASSNSHPKVWLIWMALVAAGTTRAPRVHL 331
>At2g01950.1 68415.m00130 leucine-rich repeat transmembrane protein
kinase, putative similar to brassinosteroid insensitive
protein
Length = 1143
Score = 29.9 bits (64), Expect = 2.2
Identities = 22/77 (28%), Positives = 35/77 (45%)
Frame = -1
Query: 397 PLNAKLRRRNCTSHSILYANVCNTTMKNSTPQAISTLSLPSTITHPPNDQINLLSQTLWR 218
P+ ++R R S L ++ ++ + + +LSL S T +D N+LS W
Sbjct: 5 PIRVRIRTRIQISFIFLLTHLSQSSSSDQSSLKTDSLSLLSFKTMIQDDPNNILSN--W- 61
Query: 217 GICRSPRTCPCSLSALT 167
SPR PC S +T
Sbjct: 62 ----SPRKSPCQFSGVT 74
>At1g11210.1 68414.m01284 expressed protein contains similarity to
cotton fiber expressed protein 1 [Gossypium hirsutum]
gi|3264828|gb|AAC33276
Length = 308
Score = 29.1 bits (62), Expect = 3.8
Identities = 14/39 (35%), Positives = 18/39 (46%)
Frame = -2
Query: 504 GELPSTSSVCSLRATARPRTDCALQLGPPPTPAVSLRSM 388
GE S V T RT+C L PPP P V ++ +
Sbjct: 229 GEETKQSGVLKKSETFSDRTNCYQSLPPPPPPLVKVKKV 267
>At4g04720.1 68417.m00693 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase(CDPK) [Carrot] SWISS-PROT:P28582
Length = 531
Score = 28.7 bits (61), Expect = 5.0
Identities = 17/59 (28%), Positives = 30/59 (50%)
Frame = +2
Query: 314 VLHSCITDIRVQYTMGRTVPSPQFGIERRLTAGVGGGPS*RAQSVRGRAVARREQTEDV 490
+L DIR Y++G+ + QFGI + +G G + +S+ R + ++ EDV
Sbjct: 68 ILGKPFEDIRKFYSLGKELGRGQFGI-TYMCKEIGTGNTYACKSILKRKLISKQDKEDV 125
>At2g34830.1 68415.m04276 WRKY family transcription factor
Length = 427
Score = 28.3 bits (60), Expect = 6.6
Identities = 20/69 (28%), Positives = 32/69 (46%)
Frame = -1
Query: 457 TPANRLRPSARASANTSS*PPLNAKLRRRNCTSHSILYANVCNTTMKNSTPQAISTLSLP 278
T N L S R+S+++S P + + S ++ N NS+ + +LP
Sbjct: 276 TQRNALAGSTRSSSSSSLNPSSKSSTAAATTSPSSRVFQN-------NSSKDEPNNSNLP 328
Query: 277 STITHPPND 251
S+ THPP D
Sbjct: 329 SSSTHPPFD 337
>At5g19360.1 68418.m02307 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Marchantia polymorpha] gi|5162877|dbj|BAA81748
Length = 523
Score = 27.9 bits (59), Expect = 8.7
Identities = 16/59 (27%), Positives = 28/59 (47%)
Frame = +2
Query: 314 VLHSCITDIRVQYTMGRTVPSPQFGIERRLTAGVGGGPS*RAQSVRGRAVARREQTEDV 490
VL + D++ YT+G+ + QFG+ L G +++ R + +E EDV
Sbjct: 56 VLGRPMEDVKSSYTLGKELGRGQFGV-THLCTQKATGLQFACKTIAKRKLVNKEDIEDV 113
>At4g21940.1 68417.m03174 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Nicotiana tabacum] gi|3283996|gb|AAC25423
Length = 554
Score = 27.9 bits (59), Expect = 8.7
Identities = 17/65 (26%), Positives = 30/65 (46%)
Frame = +2
Query: 296 YRLRRAVLHSCITDIRVQYTMGRTVPSPQFGIERRLTAGVGGGPS*RAQSVRGRAVARRE 475
+R +L +IR YT+G+ + QFGI G + +S+ R + R++
Sbjct: 84 FRETETILGKPFEEIRKLYTLGKELGRGQFGITYTCKEN-STGNTYACKSILKRKLTRKQ 142
Query: 476 QTEDV 490
+DV
Sbjct: 143 DIDDV 147
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,480,792
Number of Sequences: 28952
Number of extensions: 399921
Number of successful extensions: 1159
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1159
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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