Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0902
(821 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q8WYK4 Cluster: Thymidylate synthase; n=12; Deuterostom... 136 5e-31
UniRef50_Q8WYK3 Cluster: Thymidylate synthase; n=4; Eutheria|Rep... 136 5e-31
UniRef50_P04818 Cluster: Thymidylate synthase; n=129; root|Rep: ... 136 5e-31
UniRef50_Q4U9G5 Cluster: Bifunctional dihydrofolate reductase/th... 136 6e-31
UniRef50_Q2TA32 Cluster: Thymidylate synthase; n=4; cellular org... 134 2e-30
UniRef50_O96650 Cluster: Thymidylate synthase; n=14; root|Rep: T... 134 2e-30
UniRef50_Q27828 Cluster: Bifunctional dihydrofolate reductase-th... 133 4e-30
UniRef50_Q0INI0 Cluster: Os12g0446900 protein; n=11; cellular or... 131 2e-29
UniRef50_Q9UTI7 Cluster: Thymidylate synthase; n=3; Eukaryota|Re... 131 2e-29
UniRef50_P12461 Cluster: Thymidylate synthase; n=14; Ascomycota|... 126 7e-28
UniRef50_P07382 Cluster: Bifunctional dihydrofolate reductase-th... 126 7e-28
UniRef50_Q4PBV6 Cluster: Thymidylate synthase; n=1; Ustilago may... 126 9e-28
UniRef50_Q8MVZ8 Cluster: Bifunctional dihydrofolate reductase-th... 125 2e-27
UniRef50_Q07422 Cluster: Bifunctional dihydrofolate reductase-th... 123 5e-27
UniRef50_Q27713 Cluster: Bifunctional dihydrofolate reductase-th... 122 8e-27
UniRef50_Q4A320 Cluster: Bifunctional dihydrofolate reductase-th... 120 4e-26
UniRef50_Q8MVZ9 Cluster: Bifunctional dihydrofolate reductase-th... 119 8e-26
UniRef50_A3QU09 Cluster: Thymidylate synthase; n=1; Oryctes rhin... 118 2e-25
UniRef50_Q8MVZ6 Cluster: Bifunctional dihydrofolate reductase-th... 117 3e-25
UniRef50_Q8MW00 Cluster: Bifunctional dihydrofolate reductase-th... 117 4e-25
UniRef50_Q8MVZ5 Cluster: Bifunctional dihydrofolate reductase-th... 116 9e-25
UniRef50_Q5CPQ4 Cluster: Dihydrofolate reductase-thymidylate syn... 107 3e-22
UniRef50_Q8G3T9 Cluster: Thymidylate synthase; n=287; root|Rep: ... 104 2e-21
UniRef50_Q81R23 Cluster: Thymidylate synthase; n=68; cellular or... 103 5e-21
UniRef50_O33380 Cluster: Thymidylate synthase; n=116; cellular o... 102 1e-20
UniRef50_Q0FG99 Cluster: Thymidylate synthase; n=3; root|Rep: Th... 98 3e-19
UniRef50_P78029 Cluster: Thymidylate synthase; n=20; Bacteria|Re... 96 8e-19
UniRef50_Q5UQG3 Cluster: Bifunctional dihydrofolate reductase-th... 92 2e-17
UniRef50_Q9Z671 Cluster: Thymidylate synthase; n=9; Alphaproteob... 91 2e-17
UniRef50_Q76YC2 Cluster: DTMP synthase; n=1; Aeromonas phage Aeh... 87 4e-16
UniRef50_P00471 Cluster: Thymidylate synthase; n=17; Viruses|Rep... 84 4e-15
UniRef50_Q7Y427 Cluster: DTMP (Thymidylate) synthase; n=1; Enter... 84 5e-15
UniRef50_Q67JQ1 Cluster: Thymidylate synthase; n=1; Symbiobacter... 81 2e-14
UniRef50_A5KSZ1 Cluster: Thymidylate synthase; n=1; candidate di... 80 6e-14
UniRef50_Q97EV3 Cluster: Thymidylate synthase; n=2; Clostridium|... 77 4e-13
UniRef50_Q8SCS7 Cluster: PHIKZ235; n=1; Pseudomonas phage phiKZ|... 75 2e-12
UniRef50_P42326 Cluster: Thymidylate synthase A; n=9; root|Rep: ... 75 2e-12
UniRef50_Q6QGJ5 Cluster: Putative thymidylate synthase; n=1; Ent... 75 3e-12
UniRef50_Q7WP13 Cluster: Thymidylate synthase; n=43; Proteobacte... 74 4e-12
UniRef50_Q6WIC0 Cluster: DTMP (Thymidylate) synthase; n=1; Vibri... 70 8e-11
UniRef50_Q2S5G7 Cluster: Thymidylate synthase; n=8; Bacteria|Rep... 69 1e-10
UniRef50_Q934U8 Cluster: Putative thymidylate synthase; n=1; Sal... 68 2e-10
UniRef50_Q6KGF1 Cluster: Putative thymidylate synthase; n=1; Ent... 67 4e-10
UniRef50_Q3F0X9 Cluster: Thymidylate synthase; n=1; Bacillus thu... 66 8e-10
UniRef50_Q3IQP2 Cluster: Thymidylate synthase; n=4; Halobacteria... 65 2e-09
UniRef50_A5Z9U8 Cluster: Thymidylate synthase; n=9; Bacteria|Rep... 60 7e-08
UniRef50_Q8RGP6 Cluster: Thymidylate synthase; n=3; Fusobacteriu... 53 8e-06
UniRef50_Q87SA0 Cluster: Thymidylate synthase; n=76; Proteobacte... 52 2e-05
UniRef50_A2SMX7 Cluster: Putative thymidylate synthase; n=1; Met... 50 9e-05
UniRef50_Q2FCV1 Cluster: Thymidylate synthase; n=1; Acinetobacte... 42 0.019
UniRef50_Q8DUI4 Cluster: Thymidylate synthase; n=45; Streptococc... 42 0.025
UniRef50_Q8THH4 Cluster: Thymidylate synthase; n=6; Methanosarci... 41 0.033
UniRef50_Q8VBA7 Cluster: Wsv068; n=2; Shrimp white spot syndrome... 39 0.13
UniRef50_A3RUA7 Cluster: Deoxyuridylate hydroxymethyltransferase... 39 0.13
UniRef50_O26868 Cluster: Thymidylate synthase; n=3; Methanotherm... 39 0.17
UniRef50_Q1YFG6 Cluster: Possible thymidylate synthase; n=1; Aur... 37 0.53
UniRef50_A0ITX4 Cluster: Thymidylate synthase; n=1; Serratia pro... 37 0.70
UniRef50_P31654 Cluster: Deoxyuridylate hydroxymethyltransferase... 37 0.70
UniRef50_Q1VC99 Cluster: Putative uncharacterized protein; n=1; ... 36 0.93
UniRef50_Q21ZJ9 Cluster: Thymidylate synthase; n=1; Rhodoferax f... 36 1.6
UniRef50_Q86B02 Cluster: Similar to Dictyostelium discoideum (Sl... 34 4.9
UniRef50_A7TG56 Cluster: Putative uncharacterized protein; n=1; ... 34 4.9
UniRef50_UPI00015972C9 Cluster: hypothetical protein RBAM_005690... 33 6.5
UniRef50_Q4C4J0 Cluster: Thymidylate synthase; n=1; Crocosphaera... 33 6.5
UniRef50_A7TUR1 Cluster: Putative thymidylate synthase; n=1; Str... 33 8.6
>UniRef50_Q8WYK4 Cluster: Thymidylate synthase; n=12;
Deuterostomia|Rep: Thymidylate synthase - Homo sapiens
(Human)
Length = 230
Score = 136 bits (330), Expect = 5e-31
Identities = 61/91 (67%), Positives = 71/91 (78%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTL 668
GL IASY+LLT+MIAH+TG + G+FIHT GD H+YLNHIEPLKIQLQREP+ FP L
Sbjct: 137 GLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKL 196
Query: 669 EFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
RK+E IDDFK EDF I+ Y PHP I+ME
Sbjct: 197 RILRKVEKIDDFKAEDFQIEGYNPHPTIKME 227
Score = 124 bits (299), Expect = 3e-27
Identities = 53/74 (71%), Positives = 61/74 (82%)
Frame = +1
Query: 286 DYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCL 465
DY+GQG+DQLQ VID+IK NP DRR+++CAWN DL MALPPCH L QFYV + +LSC
Sbjct: 69 DYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQ 128
Query: 466 LYQRSADMGLGVPF 507
LYQRS DMGLGVPF
Sbjct: 129 LYQRSGDMGLGVPF 142
>UniRef50_Q8WYK3 Cluster: Thymidylate synthase; n=4; Eutheria|Rep:
Thymidylate synthase - Homo sapiens (Human)
Length = 279
Score = 136 bits (330), Expect = 5e-31
Identities = 61/91 (67%), Positives = 71/91 (78%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTL 668
GL IASY+LLT+MIAH+TG + G+FIHT GD H+YLNHIEPLKIQLQREP+ FP L
Sbjct: 186 GLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKL 245
Query: 669 EFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
RK+E IDDFK EDF I+ Y PHP I+ME
Sbjct: 246 RILRKVEKIDDFKAEDFQIEGYNPHPTIKME 276
Score = 133 bits (322), Expect = 4e-30
Identities = 60/84 (71%), Positives = 71/84 (84%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG RYSL + PLLTTKRVF +GV+EELLW I GST++K L++KGV IWDANGSR+
Sbjct: 58 VFGMQARYSLRDE-FPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRD 116
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
FLD+LGF+ REEGDLGPVYGFQWR
Sbjct: 117 FLDSLGFSTREEGDLGPVYGFQWR 140
Score = 71.3 bits (167), Expect = 3e-11
Identities = 31/41 (75%), Positives = 33/41 (80%)
Frame = +1
Query: 385 SDLKKMALPPCHCLAQFYVADGKLSCLLYQRSADMGLGVPF 507
SDL MALPPCH L QFYV + +LSC LYQRS DMGLGVPF
Sbjct: 151 SDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPF 191
>UniRef50_P04818 Cluster: Thymidylate synthase; n=129; root|Rep:
Thymidylate synthase - Homo sapiens (Human)
Length = 313
Score = 136 bits (330), Expect = 5e-31
Identities = 61/91 (67%), Positives = 71/91 (78%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTL 668
GL IASY+LLT+MIAH+TG + G+FIHT GD H+YLNHIEPLKIQLQREP+ FP L
Sbjct: 220 GLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKL 279
Query: 669 EFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
RK+E IDDFK EDF I+ Y PHP I+ME
Sbjct: 280 RILRKVEKIDDFKAEDFQIEGYNPHPTIKME 310
Score = 133 bits (322), Expect = 4e-30
Identities = 60/84 (71%), Positives = 71/84 (84%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG RYSL + PLLTTKRVF +GV+EELLW I GST++K L++KGV IWDANGSR+
Sbjct: 58 VFGMQARYSLRDE-FPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRD 116
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
FLD+LGF+ REEGDLGPVYGFQWR
Sbjct: 117 FLDSLGFSTREEGDLGPVYGFQWR 140
Score = 133 bits (322), Expect = 4e-30
Identities = 57/83 (68%), Positives = 68/83 (81%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA+Y D ++DY+GQG+DQLQ VID+IK NP DRR+++CAWN DL MALPPCH L QFY
Sbjct: 143 GAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFY 202
Query: 439 VADGKLSCLLYQRSADMGLGVPF 507
V + +LSC LYQRS DMGLGVPF
Sbjct: 203 VVNSELSCQLYQRSGDMGLGVPF 225
>UniRef50_Q4U9G5 Cluster: Bifunctional dihydrofolate
reductase/thymidilate synthase, putative; n=4;
Piroplasmida|Rep: Bifunctional dihydrofolate
reductase/thymidilate synthase, putative - Theileria
annulata
Length = 523
Score = 136 bits (329), Expect = 6e-31
Identities = 58/83 (69%), Positives = 69/83 (83%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GAKYID DYT QGIDQL VI+ IKNNP +RR+++C+WN +DL KMALPPCH L QF+
Sbjct: 353 GAKYIDMHADYTDQGIDQLSLVIERIKNNPDNRRLIVCSWNVADLSKMALPPCHLLFQFF 412
Query: 439 VADGKLSCLLYQRSADMGLGVPF 507
VADG LSC+++QRS DMGLGVPF
Sbjct: 413 VADGTLSCMMHQRSCDMGLGVPF 435
Score = 118 bits (285), Expect = 1e-25
Identities = 53/83 (63%), Positives = 63/83 (75%)
Frame = +2
Query: 5 FGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNF 184
FG R+ L + + PLLTTK+VF RG+IEEL+W I GST+ L K V IW+ NG+R+F
Sbjct: 269 FGYQMRFDL-SQSFPLLTTKKVFLRGIIEELIWFIRGSTNGNELLKKNVRIWELNGARSF 327
Query: 185 LDNLGFTDREEGDLGPVYGFQWR 253
LDNLGF DREE DLGPVYGFQWR
Sbjct: 328 LDNLGFYDREEQDLGPVYGFQWR 350
Score = 105 bits (251), Expect = 2e-21
Identities = 46/96 (47%), Positives = 65/96 (67%)
Frame = +3
Query: 474 KKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPK 653
+ C GL IASYSLLT+MIAH+ G + GEF+H GD H+Y NHI+ +K Q+ R P
Sbjct: 425 RSCDMGLGVPFNIASYSLLTYMIAHICGLKPGEFVHNLGDAHIYNNHIDAIKEQISRVPY 484
Query: 654 QFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
FPTL+ ++I I+DF +D +++Y+ HP I+ME
Sbjct: 485 PFPTLKLNKEITRIEDFTIDDIKVENYRCHPLIKME 520
>UniRef50_Q2TA32 Cluster: Thymidylate synthase; n=4; cellular
organisms|Rep: Thymidylate synthase - Bos taurus
(Bovine)
Length = 354
Score = 134 bits (325), Expect = 2e-30
Identities = 57/83 (68%), Positives = 68/83 (81%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA+Y D ++Y+GQG+DQLQ VID+IK NP DRR+++CAWN DL MALPPCH L QFY
Sbjct: 146 GAEYKDMDSEYSGQGVDQLQKVIDTIKTNPNDRRIILCAWNPKDLPLMALPPCHALCQFY 205
Query: 439 VADGKLSCLLYQRSADMGLGVPF 507
V +G+LSC LYQRS DMGLGVPF
Sbjct: 206 VVNGELSCQLYQRSGDMGLGVPF 228
Score = 132 bits (319), Expect = 1e-29
Identities = 59/84 (70%), Positives = 70/84 (83%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG RY+L + PLLTTKRVF +GV+EELLW I GST++K L++KGV IWDANGSR+
Sbjct: 61 VFGMQARYNLRDE-FPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRD 119
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
FLD LGF+DR EGDLGPVYGFQWR
Sbjct: 120 FLDGLGFSDRAEGDLGPVYGFQWR 143
Score = 73.3 bits (172), Expect(2) = 1e-23
Identities = 32/53 (60%), Positives = 39/53 (73%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQRE 647
GL IASY+LLT+MIAH+T + G+F+HT GD H+YLNHIEPLK Q E
Sbjct: 223 GLGVPFNIASYALLTYMIAHITDLKPGDFVHTLGDAHIYLNHIEPLKTQALME 275
Score = 60.1 bits (139), Expect(2) = 1e-23
Identities = 25/43 (58%), Positives = 34/43 (79%)
Frame = +3
Query: 633 QLQREPKQFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
+LQREP+ FP L+ RK+E+IDDF+ EDF I+ Y P+P I+ME
Sbjct: 309 ELQREPRPFPKLKILRKVETIDDFQAEDFQIEGYNPNPTIKME 351
>UniRef50_O96650 Cluster: Thymidylate synthase; n=14; root|Rep:
Thymidylate synthase - Ascaris suum (Pig roundworm)
(Ascaris lumbricoides)
Length = 294
Score = 134 bits (325), Expect = 2e-30
Identities = 58/84 (69%), Positives = 67/84 (79%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG YSL N +PLLTTKRV+ +GV+EELLW I G TD+K L+AKGV IWDANGSR
Sbjct: 38 LFGLHSTYSLRNGVIPLLTTKRVYWKGVVEELLWFIKGDTDAKRLSAKGVKIWDANGSRE 97
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
FLD+ GF DR EGDLGP+YGFQWR
Sbjct: 98 FLDSQGFKDRPEGDLGPIYGFQWR 121
Score = 121 bits (291), Expect = 3e-26
Identities = 54/83 (65%), Positives = 63/83 (75%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA+Y DY GQG+DQL VI+ IKNNP RR+++ AWN DL +MALPPCH LAQF
Sbjct: 124 GAEYHGTDADYKGQGVDQLADVIEQIKNNPNSRRIILNAWNVKDLHQMALPPCHTLAQFA 183
Query: 439 VADGKLSCLLYQRSADMGLGVPF 507
V +G+LSC LYQRS DMGLGVPF
Sbjct: 184 VKNGELSCQLYQRSGDMGLGVPF 206
Score = 100 bits (239), Expect = 5e-20
Identities = 47/90 (52%), Positives = 57/90 (63%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTL 668
GL +ASY LLTHMIAHV + G H GD HVYLNHI+ LK QL R+P+ FPT+
Sbjct: 201 GLGVPFNLASYGLLTHMIAHVCALKTGFLHHVLGDAHVYLNHIDALKEQLSRDPRPFPTV 260
Query: 669 EFARKIESIDDFKYEDFIIKDYKPHPKIEM 758
F +I+SIDDF E I+ Y P I+M
Sbjct: 261 HFEGRIDSIDDFTAESIILNGYNPMAPIKM 290
>UniRef50_Q27828 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase (DHFR-TS) [Includes:
Dihydrofolate reductase (EC 1.5.1.3); Thymidylate
synthase (EC 2.1.1.45)]; n=15; Paramecium|Rep:
Bifunctional dihydrofolate reductase-thymidylate
synthase (DHFR-TS) [Includes: Dihydrofolate reductase
(EC 1.5.1.3); Thymidylate synthase (EC 2.1.1.45)] -
Paramecium tetraurelia
Length = 462
Score = 133 bits (322), Expect = 4e-30
Identities = 53/83 (63%), Positives = 70/83 (84%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GAKY DCQTDY+ QG+DQ++ +I +KNNP RR+++ AWN DLK+MALPPCH ++QF+
Sbjct: 293 GAKYKDCQTDYSNQGVDQVKEIIQLLKNNPDSRRIILSAWNPIDLKQMALPPCHVMSQFF 352
Query: 439 VADGKLSCLLYQRSADMGLGVPF 507
VA+GKLSC++YQRS D GLG+PF
Sbjct: 353 VANGKLSCMMYQRSCDFGLGIPF 375
Score = 111 bits (266), Expect = 3e-23
Identities = 49/84 (58%), Positives = 62/84 (73%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
IFG R++L + PLLTTK+VF RGV+EELLW + G+T+ K L KGV IW+ NG+R
Sbjct: 208 IFGQTMRFNLAQS-FPLLTTKKVFFRGVVEELLWFLRGNTNGKLLLDKGVKIWEGNGTRE 266
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
+LD +G R+E DLGPVYGFQWR
Sbjct: 267 YLDTIGLQHRQEHDLGPVYGFQWR 290
Score = 93.1 bits (221), Expect = 8e-18
Identities = 44/100 (44%), Positives = 62/100 (62%)
Frame = +3
Query: 462 LVVPKKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQ 641
++ + C GL IASY+LLT+M+A GEF+H GDTH+Y NH+E LK Q++
Sbjct: 361 MMYQRSCDFGLGIPFNIASYALLTYMLAKECNLNLGEFVHVLGDTHIYSNHVEALKKQIE 420
Query: 642 REPKQFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
R P FP L+ + +S+ D+ YEDF + Y H KIEM+
Sbjct: 421 RVPYPFPLLKI-KGNKSLFDYTYEDFELVGYNAHDKIEMK 459
>UniRef50_Q0INI0 Cluster: Os12g0446900 protein; n=11; cellular
organisms|Rep: Os12g0446900 protein - Oryza sativa
subsp. japonica (Rice)
Length = 571
Score = 131 bits (317), Expect = 2e-29
Identities = 56/81 (69%), Positives = 65/81 (80%)
Frame = +1
Query: 265 KYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVA 444
+Y D DY G+G DQL VID IKNNP DRR+++ AWN +DLKKMALPPCH AQFYV
Sbjct: 397 RYTDMHADYVGKGFDQLMDVIDKIKNNPDDRRIILSAWNPTDLKKMALPPCHMFAQFYVE 456
Query: 445 DGKLSCLLYQRSADMGLGVPF 507
+G+LSC +YQRSADMGLGVPF
Sbjct: 457 NGELSCQMYQRSADMGLGVPF 477
Score = 90.2 bits (214), Expect = 5e-17
Identities = 43/75 (57%), Positives = 52/75 (69%)
Frame = +2
Query: 5 FGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNF 184
FG R++L N PLLTTKRVF RGV+EELLW ISGST++K L KG+HIWD N SR +
Sbjct: 336 FGCQMRFNLRRN-FPLLTTKRVFWRGVLEELLWFISGSTNAKVLQEKGIHIWDGNASRQY 394
Query: 185 LDNLGFTDREEGDLG 229
LD +TD +G
Sbjct: 395 LDR--YTDMHADYVG 407
Score = 88.2 bits (209), Expect = 2e-16
Identities = 43/91 (47%), Positives = 55/91 (60%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTL 668
GL IASYSLLT MIA V G+F+H GD HVY H+E L+ Q++++PK FP L
Sbjct: 472 GLGVPFNIASYSLLTCMIAQVCDLSPGDFVHVIGDAHVYRTHVEALEEQMRKQPKPFPIL 531
Query: 669 EFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
+ + ID F DF + Y PH KIEM+
Sbjct: 532 KINPVKKDIDSFVTSDFKLVRYDPHHKIEMK 562
>UniRef50_Q9UTI7 Cluster: Thymidylate synthase; n=3; Eukaryota|Rep:
Thymidylate synthase - Schizosaccharomyces pombe
(Fission yeast)
Length = 625
Score = 131 bits (317), Expect = 2e-29
Identities = 56/78 (71%), Positives = 65/78 (83%)
Frame = +2
Query: 20 RYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLG 199
R+SL NNTLPLLTTKRVF RGV+EELLW I G T++ L+ KG+HIWD NGSR FLD+ G
Sbjct: 365 RFSLRNNTLPLLTTKRVFLRGVLEELLWFIHGDTNANHLSEKGIHIWDGNGSREFLDSRG 424
Query: 200 FTDREEGDLGPVYGFQWR 253
TDR+ GDLGP+YGFQWR
Sbjct: 425 LTDRKVGDLGPIYGFQWR 442
Score = 113 bits (272), Expect = 5e-24
Identities = 52/90 (57%), Positives = 67/90 (74%), Gaps = 7/90 (7%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA+Y+DC TDYT +G+DQL VI ++K NP DRR+++ AWN + +MALPPCH QFY
Sbjct: 445 GAQYVDCDTDYTNKGVDQLAQVISTLKLNPYDRRIILSAWNPLAIPEMALPPCHIFCQFY 504
Query: 439 VAD-----GK--LSCLLYQRSADMGLGVPF 507
V++ GK LS ++YQRSADMGLGVPF
Sbjct: 505 VSEPCKPGGKPQLSSMMYQRSADMGLGVPF 534
Score = 103 bits (248), Expect = 4e-21
Identities = 49/94 (52%), Positives = 64/94 (68%), Gaps = 3/94 (3%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTL 668
GL IASYSLLTHMIAH+ GY+A EF+H GD H+Y +H+E L+ QL+R PK FP L
Sbjct: 529 GLGVPFNIASYSLLTHMIAHMCGYEAAEFVHVMGDCHIYNDHLEALQTQLERVPKAFPKL 588
Query: 669 EF---ARKIESIDDFKYEDFIIKDYKPHPKIEME 761
F A+ I SID F +DF ++ Y P+ I+M+
Sbjct: 589 FFKRDAKDIGSIDSFSVDDFAVEGYNPYGPIKMK 622
>UniRef50_P12461 Cluster: Thymidylate synthase; n=14;
Ascomycota|Rep: Thymidylate synthase - Candida albicans
(Yeast)
Length = 315
Score = 126 bits (304), Expect = 7e-28
Identities = 53/78 (67%), Positives = 64/78 (82%)
Frame = +2
Query: 20 RYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLG 199
R+ L N+T PLLTTK+VF++G+I ELLW ++GSTD+K L+ KGV IW+ NGSR FLD LG
Sbjct: 44 RFDLSNDTFPLLTTKKVFSKGIIHELLWFVAGSTDAKILSEKGVKIWEGNGSREFLDKLG 103
Query: 200 FTDREEGDLGPVYGFQWR 253
T R EGDLGPVYGFQWR
Sbjct: 104 LTHRREGDLGPVYGFQWR 121
Score = 115 bits (277), Expect = 1e-24
Identities = 58/100 (58%), Positives = 64/100 (64%), Gaps = 17/100 (17%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA+Y DC +DYTGQG DQLQ VI +K NP DRR+++ AWN D KMALPPCH QFY
Sbjct: 124 GAEYKDCDSDYTGQGFDQLQDVIKKLKTNPYDRRIIMSAWNPPDFAKMALPPCHVFCQFY 183
Query: 439 V-----------------ADGKLSCLLYQRSADMGLGVPF 507
V A KLSCLLYQRS DMGLGVPF
Sbjct: 184 VNFPTSSPDPNNPKQAKTAKPKLSCLLYQRSCDMGLGVPF 223
Score = 112 bits (269), Expect = 1e-23
Identities = 57/104 (54%), Positives = 70/104 (67%), Gaps = 4/104 (3%)
Frame = +3
Query: 462 LVVPKKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQ 641
L+ + C GL IASY+LLT MIAHV GEFIHT GD HVYL+HI+ LK Q +
Sbjct: 209 LLYQRSCDMGLGVPFNIASYALLTKMIAHVVDMDCGEFIHTLGDAHVYLDHIDALKEQFE 268
Query: 642 REPKQFPTL----EFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
R PKQFP L E +I+SIDDFK+EDF I Y+P+P I+M+
Sbjct: 269 RIPKQFPKLVIKEERKNEIKSIDDFKFEDFEIVGYEPYPPIKMK 312
>UniRef50_P07382 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase (DHFR-TS) [Includes:
Dihydrofolate reductase (EC 1.5.1.3); Thymidylate
synthase (EC 2.1.1.45)]; n=28; Trypanosomatidae|Rep:
Bifunctional dihydrofolate reductase-thymidylate
synthase (DHFR-TS) [Includes: Dihydrofolate reductase
(EC 1.5.1.3); Thymidylate synthase (EC 2.1.1.45)] -
Leishmania major
Length = 520
Score = 126 bits (304), Expect = 7e-28
Identities = 55/84 (65%), Positives = 65/84 (77%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FGA R+SL +N LPLLTTKRVF RGV EELLW + G T ++ LA K +HIWD NGSR
Sbjct: 262 LFGAQMRFSLRDNRLPLLTTKRVFWRGVCEELLWFLRGETSAQLLADKDIHIWDGNGSRE 321
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
FLD+ G T+ +E DLGPVYGFQWR
Sbjct: 322 FLDSRGLTENKEMDLGPVYGFQWR 345
Score = 114 bits (274), Expect = 3e-24
Identities = 50/85 (58%), Positives = 65/85 (76%), Gaps = 2/85 (2%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA Y + +Y G+G+DQ++ ++++IK NP DRR+L+ AWN L+KMALPPCH LAQFY
Sbjct: 348 GADYKGFEANYDGEGVDQIKLIVETIKTNPNDRRLLVTAWNPCALQKMALPPCHLLAQFY 407
Query: 439 V--ADGKLSCLLYQRSADMGLGVPF 507
V +LSC+LYQRS DMGLGVPF
Sbjct: 408 VNTDTSELSCMLYQRSCDMGLGVPF 432
Score = 103 bits (246), Expect = 7e-21
Identities = 47/104 (45%), Positives = 66/104 (63%)
Frame = +3
Query: 450 EIILLVVPKKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLK 629
E+ ++ + C GL IASY+LLT +IA TG + GE +HT GD HVY NH++ LK
Sbjct: 414 ELSCMLYQRSCDMGLGVPFNIASYALLTILIAKATGLRPGELVHTLGDAHVYRNHVDALK 473
Query: 630 IQLQREPKQFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
QL+R P FPTL F + + ++D++ D + DY PHP I+ME
Sbjct: 474 AQLERVPHAFPTLIFKEERQYLEDYELTDMEVIDYVPHPAIKME 517
>UniRef50_Q4PBV6 Cluster: Thymidylate synthase; n=1; Ustilago
maydis|Rep: Thymidylate synthase - Ustilago maydis (Smut
fungus)
Length = 554
Score = 126 bits (303), Expect = 9e-28
Identities = 55/78 (70%), Positives = 61/78 (78%)
Frame = +2
Query: 20 RYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLG 199
R+ L N TLPLLTTKRVF RGV+EELLW + G TD+K L K VHIWD NGSR FLD+ G
Sbjct: 294 RFDLTNGTLPLLTTKRVFFRGVLEELLWFVGGKTDAKLLTEKSVHIWDGNGSRAFLDSRG 353
Query: 200 FTDREEGDLGPVYGFQWR 253
+ R EGDLGPVYGFQWR
Sbjct: 354 LSHRREGDLGPVYGFQWR 371
Score = 122 bits (294), Expect = 1e-26
Identities = 58/93 (62%), Positives = 69/93 (74%), Gaps = 10/93 (10%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA Y DC TDY GQG+DQL SVID I+NNP DRR+L+ AWN +DL+ MALPPCH AQF+
Sbjct: 374 GATYKDCDTDYAGQGVDQLASVIDKIRNNPTDRRILLSAWNPADLELMALPPCHMFAQFF 433
Query: 439 VAD--------GK--LSCLLYQRSADMGLGVPF 507
V++ GK LSC +YQRS D+GLGVPF
Sbjct: 434 VSNLDAYSKGQGKKHLSCQMYQRSCDLGLGVPF 466
Score = 102 bits (244), Expect = 1e-20
Identities = 46/96 (47%), Positives = 65/96 (67%)
Frame = +3
Query: 474 KKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPK 653
+ C GL IASY+LLT+MIA VT + E I GD HVY +H++PLK QL+R+P
Sbjct: 456 RSCDLGLGVPFNIASYALLTYMIAKVTDCEPKELILAMGDAHVYKDHVQPLKAQLERDPL 515
Query: 654 QFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
FP L+ R ++ IDDF ++DF+++ YK H KI+M+
Sbjct: 516 PFPKLKIERDVKDIDDFTFDDFVVQGYKCHGKIDMK 551
>UniRef50_Q8MVZ8 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase; n=1; Chlamydaster
sterni|Rep: Bifunctional dihydrofolate
reductase-thymidylate synthase - Chlamydaster sterni
Length = 260
Score = 125 bits (301), Expect = 2e-27
Identities = 54/84 (64%), Positives = 65/84 (77%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
IFG R+S ++TLPLLTTK+VF RGV EE+LW + G TD++ L+AK +HIWD N SR
Sbjct: 173 IFGTQMRFSPRDDTLPLLTTKKVFWRGVAEEMLWFMKGCTDARVLSAKKIHIWDDNASRK 232
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
FLD G + REEGDLGPVYGFQWR
Sbjct: 233 FLDENGLSHREEGDLGPVYGFQWR 256
>UniRef50_Q07422 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase (DHFR-TS) [Includes:
Dihydrofolate reductase (EC 1.5.1.3); Thymidylate
synthase (EC 2.1.1.45)]; n=1; Toxoplasma gondii|Rep:
Bifunctional dihydrofolate reductase-thymidylate
synthase (DHFR-TS) [Includes: Dihydrofolate reductase
(EC 1.5.1.3); Thymidylate synthase (EC 2.1.1.45)] -
Toxoplasma gondii
Length = 610
Score = 123 bits (297), Expect = 5e-27
Identities = 55/84 (65%), Positives = 66/84 (78%), Gaps = 1/84 (1%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA Y D TDYTGQG+DQL++VI ++ NP DRRML+ AWN + L +MALPPCH L QFY
Sbjct: 437 GAAYKDMHTDYTGQGVDQLKNVIQMLRTNPTDRRMLMTAWNPAALDEMALPPCHLLCQFY 496
Query: 439 VADGK-LSCLLYQRSADMGLGVPF 507
V D K LSC++YQRS D+GLGVPF
Sbjct: 497 VNDQKELSCIMYQRSCDVGLGVPF 520
Score = 105 bits (253), Expect = 1e-21
Identities = 49/83 (59%), Positives = 58/83 (69%)
Frame = +2
Query: 5 FGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNF 184
FG RYSL + PLLTTKRVF +GV+EELLW I G T++ L+ KGV IWD N +R F
Sbjct: 353 FGCTMRYSL-DQAFPLLTTKRVFWKGVLEELLWFIRGDTNANHLSEKGVKIWDKNVTREF 411
Query: 185 LDNLGFTDREEGDLGPVYGFQWR 253
LD+ RE GD+GP YGFQWR
Sbjct: 412 LDSRNLPHREVGDIGPGYGFQWR 434
Score = 103 bits (248), Expect = 4e-21
Identities = 52/106 (49%), Positives = 67/106 (63%), Gaps = 2/106 (1%)
Frame = +3
Query: 450 EIILLVVPKKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLK 629
E+ ++ + C GL IASYSLLT M+AHV + EFIH G+THVY NH+E LK
Sbjct: 502 ELSCIMYQRSCDVGLGVPFNIASYSLLTLMVAHVCNLKPKEFIHFMGNTHVYTNHVEALK 561
Query: 630 IQLQREPKQFPTLEFARK--IESIDDFKYEDFIIKDYKPHPKIEME 761
QL+REP+ FP + K I+ IDDF EDF + Y PH +I+ME
Sbjct: 562 EQLRREPRPFPIVNILNKERIKEIDDFTAEDFEVVGYVPHGRIQME 607
>UniRef50_Q27713 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase (DHFR-TS) [Includes:
Dihydrofolate reductase (EC 1.5.1.3); Thymidylate
synthase (EC 2.1.1.45)]; n=49; Plasmodium|Rep:
Bifunctional dihydrofolate reductase-thymidylate
synthase (DHFR-TS) [Includes: Dihydrofolate reductase
(EC 1.5.1.3); Thymidylate synthase (EC 2.1.1.45)] -
Plasmodium berghei (strain Anka)
Length = 587
Score = 122 bits (295), Expect = 8e-27
Identities = 50/83 (60%), Positives = 67/83 (80%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA+Y D +Y +G+DQL+++I+ IKN+P RR+++CAWN +L +MALPPCH L QFY
Sbjct: 417 GAEYTDMHDNYKDKGVDQLKNIINLIKNDPTCRRIILCAWNVKNLDQMALPPCHILCQFY 476
Query: 439 VADGKLSCLLYQRSADMGLGVPF 507
V DGKLSC++YQRS D+GLGVPF
Sbjct: 477 VFDGKLSCIMYQRSCDLGLGVPF 499
Score = 107 bits (257), Expect = 3e-22
Identities = 48/83 (57%), Positives = 58/83 (69%)
Frame = +2
Query: 5 FGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNF 184
FG M +++L N PLLTTK++F RG+IEELLW I G T+ L K V IW+ANG+R F
Sbjct: 333 FGYMMKFNL-NEYFPLLTTKKLFIRGIIEELLWFIRGETNGNTLLEKNVRIWEANGTREF 391
Query: 185 LDNLGFTDREEGDLGPVYGFQWR 253
LDN RE DLGP+YGFQWR
Sbjct: 392 LDNRKLFHREVNDLGPIYGFQWR 414
Score = 101 bits (243), Expect = 2e-20
Identities = 49/100 (49%), Positives = 63/100 (63%)
Frame = +3
Query: 462 LVVPKKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQ 641
++ + C GL IASYS+ T+MIA V QA EFIH G+ HVY NHIE LKIQL
Sbjct: 485 IMYQRSCDLGLGVPFNIASYSIFTYMIAQVCNLQAAEFIHVLGNAHVYNNHIESLKIQLN 544
Query: 642 REPKQFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
R P FPTL+ I++I+DF DF +++Y H KI M+
Sbjct: 545 RTPYPFPTLKLNPDIKNIEDFTISDFTVQNYVHHDKINMD 584
>UniRef50_Q4A320 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase; n=1; Emiliania huxleyi
virus 86|Rep: Bifunctional dihydrofolate
reductase-thymidylate synthase - Emiliania huxleyi virus
86
Length = 480
Score = 120 bits (289), Expect = 4e-26
Identities = 53/83 (63%), Positives = 62/83 (74%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA Y Y QG DQLQ+VID IKNNP RR+++ AWN + L+KMALPPCH + QFY
Sbjct: 310 GADYKTMHDSYDSQGTDQLQNVIDQIKNNPTSRRIIMSAWNPNALEKMALPPCHLMCQFY 369
Query: 439 VADGKLSCLLYQRSADMGLGVPF 507
V DG LSC +YQRSAD+GLGVPF
Sbjct: 370 VHDGMLSCQMYQRSADIGLGVPF 392
Score = 120 bits (288), Expect = 6e-26
Identities = 52/84 (61%), Positives = 65/84 (77%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG R++L + PLLTTKR + +G++EELLW I GST++ LAAK V IWD NGSR+
Sbjct: 225 LFGETMRFNLSTH-FPLLTTKRTYWKGIVEELLWFIRGSTNANELAAKNVKIWDGNGSRD 283
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
FLD++G D EEGDLGPVYGFQWR
Sbjct: 284 FLDSVGLVDNEEGDLGPVYGFQWR 307
Score = 100 bits (239), Expect = 5e-20
Identities = 47/91 (51%), Positives = 59/91 (64%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTL 668
GL IASY+LLT +IAH T + GEFIH GD HVY NHI+ LK QL+R+P FPT+
Sbjct: 387 GLGVPFNIASYALLTCLIAHFTNLKPGEFIHVIGDAHVYTNHIDALKTQLKRKPYDFPTI 446
Query: 669 EFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
++I D +D I+ DYK HPKI M+
Sbjct: 447 HINNMPKTIFDVTSDDIILNDYKCHPKIHMD 477
>UniRef50_Q8MVZ9 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase; n=1; Cercomonas
longicauda|Rep: Bifunctional dihydrofolate
reductase-thymidylate synthase - Cercomonas longicauda
Length = 314
Score = 119 bits (287), Expect = 8e-26
Identities = 50/84 (59%), Positives = 64/84 (76%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG M ++SL N +PLLT +++F +GV+EELLWM+ G T S+ L KGV+IW ANGSR
Sbjct: 174 VFGNMMKFSLENGQIPLLTIRKIFWKGVVEELLWMLRGKTHSRDLEEKGVNIWRANGSRE 233
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
LD LG DRE GDLGP+YG+QWR
Sbjct: 234 ALDRLGMVDREVGDLGPIYGWQWR 257
Score = 75.4 bits (177), Expect = 2e-12
Identities = 31/56 (55%), Positives = 41/56 (73%), Gaps = 1/56 (1%)
Frame = +1
Query: 256 SGAKYIDCQTDYTGQGIDQLQSVIDSIKNNPA-DRRMLICAWNSSDLKKMALPPCH 420
SGA Y TDY+G G+DQ++ VI ++N P +RR+L C+WN DLK+MALPPCH
Sbjct: 259 SGAXYTTAHTDYSGFGVDQIKDVISQLQNQPQKNRRILFCSWNPKDLKQMALPPCH 314
>UniRef50_A3QU09 Cluster: Thymidylate synthase; n=1; Oryctes
rhinoceros virus|Rep: Thymidylate synthase - Oryctes
rhinoceros virus
Length = 321
Score = 118 bits (283), Expect = 2e-25
Identities = 53/85 (62%), Positives = 65/85 (76%), Gaps = 2/85 (2%)
Frame = +1
Query: 259 GAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFY 438
GA Y+DC TDYT +G+DQLQ VI +IK +P RR+++ AWN DL KMALPPCH L QF+
Sbjct: 143 GASYVDCNTDYTNKGVDQLQQVIRTIKEDPTSRRIIMSAWNPVDLDKMALPPCHILVQFH 202
Query: 439 V--ADGKLSCLLYQRSADMGLGVPF 507
V +G+LS LYQRSAD+ LGVPF
Sbjct: 203 VNTVNGELSAQLYQRSADLMLGVPF 227
Score = 89.0 bits (211), Expect = 1e-16
Identities = 40/84 (47%), Positives = 52/84 (61%), Gaps = 1/84 (1%)
Frame = +2
Query: 5 FGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNF 184
FG R+ L N LPLLTTK + + ++ EL W + G T+ K L + VHIW N SR +
Sbjct: 57 FGHQLRFDLRNGVLPLLTTKPMPFKTILRELFWFLRGETNQKILERQNVHIWKGNSSREY 116
Query: 185 LDNLGFTDREEGD-LGPVYGFQWR 253
LD+ G D E + LGP+YGFQWR
Sbjct: 117 LDSRGLYDYTEYESLGPIYGFQWR 140
Score = 67.7 bits (158), Expect = 3e-10
Identities = 38/91 (41%), Positives = 55/91 (60%), Gaps = 8/91 (8%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEF----A 677
IASYS+L H+IA + G + G FIH+ GD H+Y NH+ K QL REP++ P L
Sbjct: 229 IASYSILVHLIAKICGLKPGYFIHSLGDVHIYENHVSGAKEQLTREPQRQPYLNIDIDPT 288
Query: 678 RKIESI-DDFKYEDFIIKDYK--PHP-KIEM 758
++SI + + F + +YK P+P K+EM
Sbjct: 289 LDLDSILEQLSCDQFKLVNYKYNPNPIKLEM 319
>UniRef50_Q8MVZ6 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase; n=1; Diplonema
ambulator|Rep: Bifunctional dihydrofolate
reductase-thymidylate synthase - Diplonema ambulator
Length = 255
Score = 117 bits (282), Expect = 3e-25
Identities = 51/82 (62%), Positives = 59/82 (71%)
Frame = +2
Query: 5 FGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNF 184
FGA R+SL + +PLLTTKRVF +GV EEL W I G T+ K L KGVHIWD NG+R F
Sbjct: 169 FGAQMRFSLRDGVIPLLTTKRVFWKGVAEELFWFIKGCTNGKDLKDKGVHIWDGNGTRAF 228
Query: 185 LDNLGFTDREEGDLGPVYGFQW 250
LD+ G DR E DLGP+YG QW
Sbjct: 229 LDSRGLPDRAEDDLGPIYGLQW 250
>UniRef50_Q8MW00 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase; n=1; Amastigomonas
debruynei|Rep: Bifunctional dihydrofolate
reductase-thymidylate synthase - Amastigomonas debruynei
Length = 259
Score = 117 bits (281), Expect = 4e-25
Identities = 52/83 (62%), Positives = 63/83 (75%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG + L + PLLTTKRVF RGV EELLW +SGST++ LAAKG+ IWD N SR
Sbjct: 173 LFGEKLEFDLGDG-FPLLTTKRVFWRGVAEELLWFVSGSTNANELAAKGIRIWDGNSSRE 231
Query: 182 FLDNLGFTDREEGDLGPVYGFQW 250
+LD++G T+RE GDLGPVYGFQW
Sbjct: 232 YLDSIGLTEREVGDLGPVYGFQW 254
>UniRef50_Q8MVZ5 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase; n=1; Tetrahymena
sp.|Rep: Bifunctional dihydrofolate
reductase-thymidylate synthase - Tetrahymena sp
Length = 311
Score = 116 bits (278), Expect = 9e-25
Identities = 51/83 (61%), Positives = 57/83 (68%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
IFGAM R+ L + PLLTTK+ F RG+ EEL W + G T LAAK VHIWDAN +R
Sbjct: 224 IFGAMMRFDLSSGAFPLLTTKKTFLRGIAEELFWFLKGDTSQTRLAAKDVHIWDANATRA 283
Query: 182 FLDNLGFTDREEGDLGPVYGFQW 250
FLD G DR EGDLGPVYG QW
Sbjct: 284 FLDARGLPDRPEGDLGPVYGCQW 306
>UniRef50_Q5CPQ4 Cluster: Dihydrofolate reductase-thymidylate
synthase; n=14; cellular organisms|Rep: Dihydrofolate
reductase-thymidylate synthase - Cryptosporidium parvum
Iowa II
Length = 531
Score = 107 bits (257), Expect = 3e-22
Identities = 48/82 (58%), Positives = 60/82 (73%), Gaps = 1/82 (1%)
Frame = +1
Query: 265 KYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVA 444
+Y DYTG G+DQL +I+++KNNP DRR ++ AWN S L +MALPPCH L+Q+YV
Sbjct: 362 EYKTMHDDYTGVGVDQLAKLIETLKNNPKDRRHILTAWNPSALSQMALPPCHVLSQYYVT 421
Query: 445 -DGKLSCLLYQRSADMGLGVPF 507
D LSC LYQRS D+GLG PF
Sbjct: 422 NDNCLSCNLYQRSCDLGLGSPF 443
Score = 104 bits (249), Expect = 3e-21
Identities = 48/96 (50%), Positives = 64/96 (66%)
Frame = +3
Query: 474 KKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPK 653
+ C GL S IASY++LT M+A V GY+ GE GD H+Y NH+ LK QL R P+
Sbjct: 433 RSCDLGLGSPFNIASYAILTMMLAQVCGYEPGELAIFIGDAHIYENHLTQLKEQLSRTPR 492
Query: 654 QFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
FP L+F RK+E+I+DFK+ED + Y P+P I+ME
Sbjct: 493 PFPQLKFKRKVENIEDFKWEDIELIGYYPYPTIKME 528
Score = 101 bits (241), Expect = 3e-20
Identities = 43/84 (51%), Positives = 56/84 (66%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
IFG M R+ + + PLLTTK+V R + EEL+W I G T+ L K V+IW NGS+
Sbjct: 275 IFGQMMRFDMRES-FPLLTTKKVAIRSIFEELIWFIKGDTNGNHLIEKKVYIWSGNGSKE 333
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
+L+ +G REE DLGP+YGFQWR
Sbjct: 334 YLERIGLGHREENDLGPIYGFQWR 357
>UniRef50_Q8G3T9 Cluster: Thymidylate synthase; n=287; root|Rep:
Thymidylate synthase - Bifidobacterium longum
Length = 266
Score = 104 bits (250), Expect = 2e-21
Identities = 45/68 (66%), Positives = 56/68 (82%)
Frame = +1
Query: 304 IDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSA 483
IDQ+ +V+D IK++P RRM++ AWN ++++KMALPPCH L QFYVAD KLSC LYQRS
Sbjct: 112 IDQISNVLDLIKHHPDSRRMIVSAWNPAEVEKMALPPCHALFQFYVADDKLSCQLYQRSC 171
Query: 484 DMGLGVPF 507
DM LGVPF
Sbjct: 172 DMFLGVPF 179
Score = 86.6 bits (205), Expect = 7e-16
Identities = 41/82 (50%), Positives = 56/82 (68%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASYSLLT M+A G + GEF+ T GD HVY NH++ QL R+P +PT+E RK +
Sbjct: 181 IASYSLLTMMMAQQAGLKPGEFVWTGGDCHVYDNHVDQFLEQLGRKPYPYPTIEI-RKAD 239
Query: 690 SIDDFKYEDFIIKDYKPHPKIE 755
S+ D++YEDF + Y+ HP I+
Sbjct: 240 SLFDYQYEDFKVVGYQHHPTIK 261
Score = 67.7 bits (158), Expect = 3e-10
Identities = 38/84 (45%), Positives = 47/84 (55%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG R+ L + PLLTTK VF +G+ ELLW + GS++ L VHIWD
Sbjct: 28 LFGQQMRFDL-SKYFPLLTTKTVFFKGLAYELLWFLKGSSNINWLLEHNVHIWD-----E 81
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
+ D E GDLGPVYG QWR
Sbjct: 82 WAD-------ENGDLGPVYGVQWR 98
>UniRef50_Q81R23 Cluster: Thymidylate synthase; n=68; cellular
organisms|Rep: Thymidylate synthase - Bacillus anthracis
Length = 318
Score = 103 bits (247), Expect = 5e-21
Identities = 44/71 (61%), Positives = 54/71 (76%)
Frame = +1
Query: 295 GQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQ 474
G+ +DQL+ VI+ IK P RR+++ AWN D+ MALPPCH L QFYVADGKLSC LYQ
Sbjct: 160 GETLDQLKDVIEMIKKTPDSRRLIVSAWNPEDVPSMALPPCHTLFQFYVADGKLSCQLYQ 219
Query: 475 RSADMGLGVPF 507
RS D+ LG+PF
Sbjct: 220 RSGDIFLGIPF 230
Score = 103 bits (246), Expect = 7e-21
Identities = 44/82 (53%), Positives = 57/82 (69%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASYSLLTH+IAH G + GEF+HT GD H+Y NH E ++ QL REP+ FP L ++
Sbjct: 232 IASYSLLTHLIAHECGLEVGEFVHTIGDAHIYTNHFEQVEKQLAREPRPFPKLTLNPDVK 291
Query: 690 SIDDFKYEDFIIKDYKPHPKIE 755
S+ DF+ ED I+ Y PHP I+
Sbjct: 292 SVFDFEMEDLTIEGYDPHPAIK 313
Score = 47.6 bits (108), Expect = 4e-04
Identities = 26/76 (34%), Positives = 41/76 (53%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG R+ L PLLTTKRV R V ELLW + G T+ + L +IW+ ++
Sbjct: 33 VFGYQMRFDLSKG-FPLLTTKRVPFRLVASELLWFMKGDTNIRYLLQHNNNIWNEWAFKS 91
Query: 182 FLDNLGFTDREEGDLG 229
++++ +T + D G
Sbjct: 92 WVESDEYTGPDMIDFG 107
>UniRef50_O33380 Cluster: Thymidylate synthase; n=116; cellular
organisms|Rep: Thymidylate synthase - Neisseria
gonorrhoeae
Length = 265
Score = 102 bits (244), Expect = 1e-20
Identities = 44/71 (61%), Positives = 56/71 (78%)
Frame = +1
Query: 295 GQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQ 474
G+ IDQ+ +V++ IK NP RR+++ AWN + + +MALPPCH L QFYVADG LSC LYQ
Sbjct: 107 GRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGNLSCQLYQ 166
Query: 475 RSADMGLGVPF 507
RSAD+ LGVPF
Sbjct: 167 RSADIFLGVPF 177
Score = 96.3 bits (229), Expect = 8e-19
Identities = 40/82 (48%), Positives = 58/82 (70%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASY+LLT M+A V G +AGEF+HT GD H+Y NH E +QL+REP+ P ++ +++
Sbjct: 179 IASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAALQLEREPRALPVMKINPEVK 238
Query: 690 SIDDFKYEDFIIKDYKPHPKIE 755
+ FK+EDF ++ Y PHP I+
Sbjct: 239 DLFAFKFEDFELEGYDPHPHIK 260
Score = 68.1 bits (159), Expect = 2e-10
Identities = 37/84 (44%), Positives = 46/84 (54%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG R+ L PLLTTK++ R +I ELLW + G T+ K L V IWD
Sbjct: 29 VFGYQMRFDLGKG-FPLLTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDDE---- 83
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
+ D E GDLGPVYG+QWR
Sbjct: 84 ------WAD-ENGDLGPVYGYQWR 100
>UniRef50_Q0FG99 Cluster: Thymidylate synthase; n=3; root|Rep:
Thymidylate synthase - alpha proteobacterium HTCC2255
Length = 292
Score = 97.9 bits (233), Expect = 3e-19
Identities = 43/67 (64%), Positives = 53/67 (79%)
Frame = +1
Query: 307 DQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSAD 486
DQ+ +I+ IKNNP RRM++ AWN + + +MALPPCH +AQF V +GKLSC LYQRSAD
Sbjct: 136 DQIIWLINEIKNNPDSRRMILSAWNPNQINEMALPPCHTMAQFRVMNGKLSCQLYQRSAD 195
Query: 487 MGLGVPF 507
M LGVPF
Sbjct: 196 MFLGVPF 202
Score = 64.5 bits (150), Expect = 3e-09
Identities = 31/84 (36%), Positives = 48/84 (57%), Gaps = 2/84 (2%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFA--RK 683
IASYSLLTHM+A + + G F+ GD H+Y NH + + QL+R PK P LE
Sbjct: 204 IASYSLLTHMLAQLCDLKVGSFVWVGGDCHIYKNHKKQVLEQLKRTPKSLPNLEMPSFSN 263
Query: 684 IESIDDFKYEDFIIKDYKPHPKIE 755
++ + + +++K+Y P I+
Sbjct: 264 LDELLQTRPIGYVLKNYDPMDSIK 287
Score = 56.4 bits (130), Expect = 8e-07
Identities = 36/96 (37%), Positives = 51/96 (53%), Gaps = 12/96 (12%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALA--------AKGVH- 154
+FG R++L P +TTK++ R ++ ELLW + GSTD + LA VH
Sbjct: 29 VFGYQIRFNLQEG-FPAVTTKKLAWRSIVGELLWFLEGSTDERRLAELTFNKDRTDLVHK 87
Query: 155 --IWDANGSRNFLDNLGFTDRE-EGDLGPVYGFQWR 253
IW AN + LG+ + + +LGPVYG QWR
Sbjct: 88 KTIWTANADAQGAE-LGYENNDLIKELGPVYGHQWR 122
>UniRef50_P78029 Cluster: Thymidylate synthase; n=20; Bacteria|Rep:
Thymidylate synthase - Mycoplasma pneumoniae
Length = 287
Score = 96.3 bits (229), Expect = 8e-19
Identities = 40/69 (57%), Positives = 53/69 (76%)
Frame = +1
Query: 301 GIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRS 480
G+DQL+ VI IK NP RR+++ +WN +++ +M LPPCH L QFYV DG+LSC LYQRS
Sbjct: 132 GVDQLKKVIAQIKVNPFSRRLIVSSWNPNEVDQMLLPPCHSLYQFYVQDGQLSCQLYQRS 191
Query: 481 ADMGLGVPF 507
D+ LG+PF
Sbjct: 192 GDIFLGIPF 200
Score = 84.6 bits (200), Expect = 3e-15
Identities = 37/84 (44%), Positives = 56/84 (66%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASYSLL +++A T + G F+HT GD H+Y NH+E +K+QL R+PK P + + +
Sbjct: 202 IASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQIKLQLTRQPKPLPKV-VLKSDK 260
Query: 690 SIDDFKYEDFIIKDYKPHPKIEME 761
SI D++++D + DY HP I+ E
Sbjct: 261 SIFDYQFDDIELVDYDHHPTIKGE 284
Score = 54.8 bits (126), Expect = 2e-06
Identities = 34/101 (33%), Positives = 52/101 (51%), Gaps = 17/101 (16%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDA----- 166
+FG + + + PLLTTK+V + ++ ELLW I G T+ K L V+IW+
Sbjct: 29 VFGYQMHFDISKH-FPLLTTKKVHWKSIVHELLWFIKGDTNIKYLVDNKVNIWNEWPYES 87
Query: 167 -------NG--SRNFLDNL---GFTDREEGDLGPVYGFQWR 253
NG + F++ + ++ G+LGPVYG QWR
Sbjct: 88 FKKSPHFNGESQKEFIERIRQDAKFAQQFGNLGPVYGKQWR 128
>UniRef50_Q5UQG3 Cluster: Bifunctional dihydrofolate
reductase-thymidylate synthase (DHFR-TS) [Includes:
Dihydrofolate reductase (EC 1.5.1.3); Thymidylate
synthase (EC 2.1.1.45)]; n=1; Acanthamoeba polyphaga
mimivirus|Rep: Bifunctional dihydrofolate
reductase-thymidylate synthase (DHFR-TS) [Includes:
Dihydrofolate reductase (EC 1.5.1.3); Thymidylate
synthase (EC 2.1.1.45)] - Mimivirus
Length = 563
Score = 91.9 bits (218), Expect = 2e-17
Identities = 50/100 (50%), Positives = 59/100 (59%), Gaps = 10/100 (10%)
Frame = +1
Query: 229 PCIWISMETSGAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMAL 408
P M GA+Y DC+T Y GQGIDQL I+SI+NNP DRR++I WN SDL KMAL
Sbjct: 373 PGYGFQMRYWGAEYTDCKTSYQGQGIDQLNKCIESIQNNPHDRRIMINLWNCSDLDKMAL 432
Query: 409 PPCHCLAQFYV----------ADGKLSCLLYQRSADMGLG 498
PCH F V G+L+C L QRS D+ LG
Sbjct: 433 APCHFCYMFGVDLYEVPTTSGKKGRLNCHLVQRSWDVLLG 472
Score = 85.0 bits (201), Expect = 2e-15
Identities = 40/84 (47%), Positives = 50/84 (59%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
IFG +Y L P+ T KR + + + EEL+WMI G TD L KGVH+WD N S++
Sbjct: 299 IFGYQLKYDLSKG-YPIQTIKRSYPKAIFEELMWMIRGQTDVSILQKKGVHVWDKNSSKD 357
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
FL EEGD+GP YGFQ R
Sbjct: 358 FLSKYNL-PYEEGDIGPGYGFQMR 380
Score = 67.3 bits (157), Expect = 4e-10
Identities = 32/82 (39%), Positives = 50/82 (60%), Gaps = 2/82 (2%)
Frame = +3
Query: 522 SLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQ--LQREPKQFPTLEFARKIESI 695
+LLT++IA+ G +H+ D H+Y +HI+ I LQR+ ++FP L K E I
Sbjct: 479 ALLTYLIANHCDLDPGILVHSISDAHIYQSHIDSGAISQLLQRKCRKFPNLVIRNKKEKI 538
Query: 696 DDFKYEDFIIKDYKPHPKIEME 761
DD++++D II++Y P P I E
Sbjct: 539 DDYEFDDLIIENYYPCPSISAE 560
>UniRef50_Q9Z671 Cluster: Thymidylate synthase; n=9;
Alphaproteobacteria|Rep: Thymidylate synthase -
Zymomonas mobilis
Length = 296
Score = 91.5 bits (217), Expect = 2e-17
Identities = 40/72 (55%), Positives = 58/72 (80%), Gaps = 1/72 (1%)
Frame = +1
Query: 295 GQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYV-ADGKLSCLLY 471
GQ DQ+ ++I+++K+NP+ RRML WN ++LK MALPPCH + Q++V +DG+L+ LLY
Sbjct: 137 GQVYDQIATLIETLKSNPSSRRMLFHGWNVAELKDMALPPCHMVYQYHVTSDGRLNSLLY 196
Query: 472 QRSADMGLGVPF 507
QRSAD+ LG+PF
Sbjct: 197 QRSADVFLGLPF 208
Score = 81.8 bits (193), Expect = 2e-14
Identities = 37/76 (48%), Positives = 46/76 (60%)
Frame = +3
Query: 528 LTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIESIDDFK 707
L M+A G G+ + T GD H+Y NHI+ +K QL REP+ FP LE R SI D+K
Sbjct: 216 LQAMLADQAGLALGDLVWTGGDVHIYRNHIDQMKEQLAREPRAFPRLELTRHPNSITDYK 275
Query: 708 YEDFIIKDYKPHPKIE 755
EDF I Y PHP I+
Sbjct: 276 IEDFAITGYDPHPPIK 291
Score = 65.7 bits (153), Expect = 1e-09
Identities = 39/100 (39%), Positives = 53/100 (53%), Gaps = 16/100 (16%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIW-DANGSR 178
+FGAM R+ L +P+LTTK+V R I E+LW +SG T+ + L +GV IW D +R
Sbjct: 31 LFGAMMRFDLSKGRIPILTTKKVSYRLAIREMLWFLSGDTNIRPLVEQGVSIWSDWPLAR 90
Query: 179 NFLDNLGFTDREE---------------GDLGPVYGFQWR 253
+ ++E GDLGPVYG QWR
Sbjct: 91 YQAETGALLSKKEFEAKILADTEFAKKWGDLGPVYGRQWR 130
>UniRef50_Q76YC2 Cluster: DTMP synthase; n=1; Aeromonas phage
Aeh1|Rep: DTMP synthase - Aeromonas phage Aeh1
Length = 277
Score = 87.4 bits (207), Expect = 4e-16
Identities = 39/69 (56%), Positives = 47/69 (68%)
Frame = +1
Query: 301 GIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRS 480
G+DQL I+ IK NP RR ++CAWN DL KMALPPCH L +F V G+L+ YQRS
Sbjct: 118 GVDQLAKAIERIKTNPECRRNIVCAWNPVDLDKMALPPCHVLFRFSVKRGRLNLTWYQRS 177
Query: 481 ADMGLGVPF 507
D LG+PF
Sbjct: 178 VDTFLGLPF 186
Score = 76.2 bits (179), Expect = 9e-13
Identities = 35/85 (41%), Positives = 53/85 (62%), Gaps = 3/85 (3%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASY LL ++A +TGY+AG DTH+Y NH E ++ QLQREP + PTL+F +
Sbjct: 188 IASYGLLLEIVAQITGYKAGILTGMLDDTHIYHNHFEQVQEQLQREPHKLPTLKFKNRFT 247
Query: 690 SIDD---FKYEDFIIKDYKPHPKIE 755
++++ +D ++DYK H I+
Sbjct: 248 TLEEACNLSVDDIELEDYKCHSAIK 272
Score = 48.8 bits (111), Expect = 2e-04
Identities = 34/91 (37%), Positives = 50/91 (54%), Gaps = 7/91 (7%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAK--GVH-----IW 160
+ G + R+ + + P +TTK + + V E LW ++G T+ + L A GV IW
Sbjct: 29 VLGQVFRHDMADG-FPAVTTKTLAWKAVRGECLWFMNGFTNVEVLRAMTYGVGSSSKTIW 87
Query: 161 DANGSRNFLDNLGFTDREEGDLGPVYGFQWR 253
D N + + LG+TD G+LGPVYG QWR
Sbjct: 88 DDNFNHQGVA-LGYTD---GELGPVYGKQWR 114
>UniRef50_P00471 Cluster: Thymidylate synthase; n=17; Viruses|Rep:
Thymidylate synthase - Bacteriophage T4
Length = 286
Score = 84.2 bits (199), Expect = 4e-15
Identities = 38/69 (55%), Positives = 47/69 (68%)
Frame = +1
Query: 301 GIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRS 480
G+DQ+ VID IK P DRR ++ AWN ++LK MALPPCH QF V +G L YQRS
Sbjct: 118 GVDQIIEVIDRIKKLPNDRRQIVSAWNPAELKYMALPPCHMFYQFNVRNGYLDLQWYQRS 177
Query: 481 ADMGLGVPF 507
D+ LG+PF
Sbjct: 178 VDVFLGLPF 186
Score = 60.1 bits (139), Expect = 7e-08
Identities = 32/94 (34%), Positives = 53/94 (56%), Gaps = 12/94 (12%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFA---- 677
IASY+ L H++A + G+ I + G+TH+Y+NH+E K L+REPK+ L +
Sbjct: 188 IASYATLVHIVAKMCNLIPGDLIFSGGNTHIYMNHVEQCKEILRREPKELCELVISGLPY 247
Query: 678 --RKIESIDDFKY------EDFIIKDYKPHPKIE 755
R + + + KY +DF++ +Y HP I+
Sbjct: 248 KFRYLSTKEQLKYVLKLRPKDFVLNNYVSHPPIK 281
Score = 55.6 bits (128), Expect = 1e-06
Identities = 33/91 (36%), Positives = 49/91 (53%), Gaps = 7/91 (7%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKAL-------AAKGVHIW 160
+FG+ R+ L P +TTK++ + I EL+W +SGST+ L +G +W
Sbjct: 29 LFGSKLRWDLTKG-FPAVTTKKLAWKACIAELIWFLSGSTNVNDLRLIQHDSLIQGKTVW 87
Query: 161 DANGSRNFLDNLGFTDREEGDLGPVYGFQWR 253
D N N +LG+ G+LGP+YG QWR
Sbjct: 88 DEN-YENQAKDLGY---HSGELGPIYGKQWR 114
>UniRef50_Q7Y427 Cluster: DTMP (Thymidylate) synthase; n=1;
Enterobacteria phage RB49|Rep: DTMP (Thymidylate)
synthase - Enterobacteria phage RB49
Length = 410
Score = 83.8 bits (198), Expect = 5e-15
Identities = 37/67 (55%), Positives = 46/67 (68%)
Frame = +1
Query: 307 DQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSAD 486
DQLQ+VI+ IKNNP DRR++I +WN L CH QFYV +GKLSC+ +QRS D
Sbjct: 254 DQLQNVIEQIKNNPTDRRIIIDSWNVQGTHNAVLGVCHPFVQFYVNEGKLSCMWFQRSVD 313
Query: 487 MGLGVPF 507
LG+PF
Sbjct: 314 SFLGLPF 320
Score = 68.9 bits (161), Expect = 1e-10
Identities = 35/84 (41%), Positives = 52/84 (61%), Gaps = 3/84 (3%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
+ASY+LLTH+IA + + GE I + GDTH+Y NH + + QL R P PT+E + +
Sbjct: 322 VASYALLTHIIAKMCDLEVGELIFSGGDTHIYKNHFDQVMEQLSRVPFDKPTIELP-EFK 380
Query: 690 SIDDF---KYEDFIIKDYKPHPKI 752
S+D+ +DF I +Y+ HP I
Sbjct: 381 SLDEVLTKTPKDFKIHNYQSHPAI 404
Score = 42.3 bits (95), Expect = 0.014
Identities = 22/44 (50%), Positives = 26/44 (59%)
Frame = +2
Query: 5 FGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKAL 136
FGA R L PLLTTK++ T VI ELLW + GS D + L
Sbjct: 30 FGAEMRIDLSKG-FPLLTTKKMATASVIGELLWFMEGSLDRRRL 72
>UniRef50_Q67JQ1 Cluster: Thymidylate synthase; n=1; Symbiobacterium
thermophilum|Rep: Thymidylate synthase - Symbiobacterium
thermophilum
Length = 283
Score = 81.4 bits (192), Expect = 2e-14
Identities = 37/84 (44%), Positives = 49/84 (58%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASYSLL HM+A VTG G + H D H+Y N ++ +++QL REP P L ++
Sbjct: 197 IASYSLLLHMVAQVTGLAVGTYTHYLNDVHIYRNLLDQVRLQLTREPYPLPRLWLNPEVR 256
Query: 690 SIDDFKYEDFIIKDYKPHPKIEME 761
ID F +D + DYK HP I E
Sbjct: 257 EIDQFTMDDIQLVDYKHHPPIRGE 280
Score = 73.7 bits (173), Expect = 5e-12
Identities = 33/69 (47%), Positives = 49/69 (71%), Gaps = 1/69 (1%)
Frame = +1
Query: 304 IDQLQSVI-DSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRS 480
I Q++ + D + +P RR+++ WN +DL++MALPPCH QF+V DG+L ++QRS
Sbjct: 127 IQQIREIAADPERPHPNSRRLVVSPWNVADLEEMALPPCHGPFQFHVKDGRLHLAVWQRS 186
Query: 481 ADMGLGVPF 507
AD+ LGVPF
Sbjct: 187 ADLFLGVPF 195
Score = 65.7 bits (153), Expect = 1e-09
Identities = 34/84 (40%), Positives = 46/84 (54%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG M ++ P LTTK++F V ELLW ++G TD + L +G HIWD + +RN
Sbjct: 39 VFGRMLKHDFKEG-FPALTTKKLFFNSVKAELLWFLAGRTDLEFLHERGCHIWDKDAARN 97
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
G LGP+YG QWR
Sbjct: 98 -----------GGKLGPIYGKQWR 110
>UniRef50_A5KSZ1 Cluster: Thymidylate synthase; n=1; candidate
division TM7 genomosp. GTL1|Rep: Thymidylate synthase -
candidate division TM7 genomosp. GTL1
Length = 280
Score = 80.2 bits (189), Expect = 6e-14
Identities = 34/84 (40%), Positives = 55/84 (65%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IA Y++L HM+A++ + E I + G H+Y N IEP K Q++R+P +FP L A ++
Sbjct: 193 IAQYAMLLHMVAYLADLEPRELIISIGSAHLYRNQIEPAKEQVRRKPYRFPKLTIAGDVK 252
Query: 690 SIDDFKYEDFIIKDYKPHPKIEME 761
IDDFK ++F ++ Y+ HP I+ +
Sbjct: 253 DIDDFKEKNFSLEGYQSHPHIKTD 276
Score = 70.1 bits (164), Expect = 6e-11
Identities = 37/81 (45%), Positives = 49/81 (60%), Gaps = 10/81 (12%)
Frame = +1
Query: 295 GQGIDQLQSVIDSIKNNPADRRMLICAWNSSDL--KK--------MALPPCHCLAQFYVA 444
G DQLQ I+ IK NP + +++ AWN+ +L K+ M LPPCH + QF V
Sbjct: 111 GSQFDQLQWAIEQIKTNPNSKAIIVSAWNAGELGDKRWNPNDYGDMRLPPCHTMFQFDVT 170
Query: 445 DGKLSCLLYQRSADMGLGVPF 507
GKL LYQRS+D+ LG+PF
Sbjct: 171 GGKLRMQLYQRSSDVFLGLPF 191
Score = 54.0 bits (124), Expect = 4e-06
Identities = 32/84 (38%), Positives = 41/84 (48%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
IFG R+ + PLLTTK++ + ++ EL W ISGS+ L +H WD
Sbjct: 33 IFGYQIRFDMRQG-FPLLTTKKMPFKILLHELFWFISGSSSVDYLHEHKIHYWD------ 85
Query: 182 FLDNLGFTDREEGDLGPVYGFQWR 253
GF DLG VYG QWR
Sbjct: 86 -----GFLHEGSDDLGRVYGVQWR 104
>UniRef50_Q97EV3 Cluster: Thymidylate synthase; n=2;
Clostridium|Rep: Thymidylate synthase - Clostridium
acetobutylicum
Length = 263
Score = 77.4 bits (182), Expect = 4e-13
Identities = 35/66 (53%), Positives = 47/66 (71%)
Frame = +1
Query: 310 QLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSADM 489
Q+ ++ID++K +P RRMLI WN +DLK M+L PC + V +GKL+C+L QRS DM
Sbjct: 108 QIDNLIDTLKTDPQSRRMLISLWNIADLKDMSLTPCCYETLWDVQNGKLNCMLVQRSGDM 167
Query: 490 GLGVPF 507
LGVPF
Sbjct: 168 PLGVPF 173
Score = 73.3 bits (172), Expect = 7e-12
Identities = 36/86 (41%), Positives = 51/86 (59%), Gaps = 2/86 (2%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQF--PTLEFARK 683
+ Y++L H+IA VTG + G F H + H+Y N IE +KIQL+R K + P L
Sbjct: 175 MCQYAVLVHLIAQVTGLKPGLFTHVINNAHIYKNQIEGMKIQLKRRDKAYEAPKLWINPN 234
Query: 684 IESIDDFKYEDFIIKDYKPHPKIEME 761
I++ DF +D + DYK H KI+ME
Sbjct: 235 IKNFYDFTADDIKLIDYKHHDKIKME 260
Score = 33.5 bits (73), Expect = 6.5
Identities = 17/40 (42%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +2
Query: 47 PLLTTKRVFTRGVIEELLWMISG-STDSKALAAKGVHIWD 163
P+LTTK V + ++ELLW+ S D + L + V IWD
Sbjct: 47 PILTTKFVAFKTSVKELLWIYKDQSNDVRKLQNENVTIWD 86
>UniRef50_Q8SCS7 Cluster: PHIKZ235; n=1; Pseudomonas phage
phiKZ|Rep: PHIKZ235 - Pseudomonas phage phiKZ
Length = 488
Score = 74.9 bits (176), Expect = 2e-12
Identities = 39/95 (41%), Positives = 54/95 (56%), Gaps = 2/95 (2%)
Frame = +3
Query: 474 KKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPK 653
+ C L I YSLLTHM+A+ A EFI T GD H+Y NH E +QL REPK
Sbjct: 388 RSCDTFLGKPFNITFYSLLTHMLANQMNMIAEEFIWTGGDVHIYSNHQEQTDLQLTREPK 447
Query: 654 QFPTLEFARKI--ESIDDFKYEDFIIKDYKPHPKI 752
PT+ F ++ + I + +DF+I++Y+ H I
Sbjct: 448 VLPTIRFKQEAIGKDIREIGVDDFVIENYEYHSHI 482
Score = 65.3 bits (152), Expect = 2e-09
Identities = 28/54 (51%), Positives = 36/54 (66%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWD 163
+FG +RYSL NN LP++TTK++ VI EL WM+SG T L A GV IW+
Sbjct: 29 MFGQQERYSLRNNRLPVVTTKKIHLASVIHELFWMLSGDTKVDYLIANGVRIWN 82
Score = 58.8 bits (136), Expect = 2e-07
Identities = 21/48 (43%), Positives = 32/48 (66%)
Frame = +1
Query: 304 IDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVAD 447
IDQ+Q +ID ++ NP RR+++CAW + + ALPPCH QF+ +
Sbjct: 261 IDQIQDIIDQLRTNPDSRRIIVCAWAPHLIDEQALPPCHAFIQFWTRE 308
>UniRef50_P42326 Cluster: Thymidylate synthase A; n=9; root|Rep:
Thymidylate synthase A - Bacillus subtilis
Length = 279
Score = 74.9 bits (176), Expect = 2e-12
Identities = 35/84 (41%), Positives = 51/84 (60%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
+ Y++L MIA VTGY+ GE+I GD HVY HI+ LKIQ++RE + P L +++
Sbjct: 193 VFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVK 252
Query: 690 SIDDFKYEDFIIKDYKPHPKIEME 761
DF +DF + +YK K+ E
Sbjct: 253 DFYDFTIDDFKLINYKHGDKLLFE 276
Score = 66.9 bits (156), Expect = 6e-10
Identities = 31/71 (43%), Positives = 42/71 (59%)
Frame = +1
Query: 295 GQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQ 474
G+ +DQ+ ++ +KNNP+ RR + WN +L MAL PC Q+YV GKL +
Sbjct: 121 GEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRA 180
Query: 475 RSADMGLGVPF 507
RS DM LG PF
Sbjct: 181 RSNDMALGNPF 191
Score = 45.2 bits (102), Expect = 0.002
Identities = 28/72 (38%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = +2
Query: 35 NNTLPLLTTKRVFTRGVIEELLWMIS-GSTDSKALAAKGVHIWDANGSRNFLDNLGFTDR 211
N+ +P+LTTK+V + I+ELLW+ S D L GVHIWD +
Sbjct: 52 NSEVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNMMGVHIWDQ------------WKQ 99
Query: 212 EEGDLGPVYGFQ 247
E+G +G YGFQ
Sbjct: 100 EDGTIGHAYGFQ 111
>UniRef50_Q6QGJ5 Cluster: Putative thymidylate synthase; n=1;
Enterobacteria phage T5|Rep: Putative thymidylate
synthase - Bacteriophage T5
Length = 279
Score = 74.5 bits (175), Expect = 3e-12
Identities = 37/70 (52%), Positives = 46/70 (65%), Gaps = 1/70 (1%)
Frame = +1
Query: 301 GIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVA-DGKLSCLLYQR 477
G DQ+ +I+ +KNNP RR L+ AWN ++L KMALPPCH Q YV+ DG L QR
Sbjct: 118 GRDQILELIEGLKNNPHGRRHLVSAWNVAELDKMALPPCHYGFQCYVSNDGYLDLKWTQR 177
Query: 478 SADMGLGVPF 507
S D LG+PF
Sbjct: 178 SVDCFLGLPF 187
Score = 65.7 bits (153), Expect = 1e-09
Identities = 31/86 (36%), Positives = 53/86 (61%), Gaps = 4/86 (4%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASY+LLTH++A +TG + I + GDTH+Y +H+E ++ Q++R+P+ PTL ++
Sbjct: 189 IASYALLTHILAKLTGLKPRYLIFSGGDTHIYNDHMEQVEEQVKRKPRPLPTLVMPEFVD 248
Query: 690 SIDDFKYE----DFIIKDYKPHPKIE 755
D + F ++ Y PHP ++
Sbjct: 249 LYDLLENNTAAWSFHLEGYDPHPALK 274
Score = 50.8 bits (116), Expect = 4e-05
Identities = 31/88 (35%), Positives = 46/88 (52%), Gaps = 4/88 (4%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALA----AKGVHIWDAN 169
IF + ++ L P +TTKR+ + V+ ELLW +SGST+ L + W
Sbjct: 29 IFDSYAKFDLREG-FPAVTTKRLAWKSVVGELLWFLSGSTNLHDLRVFTFGRDEGQWTI- 86
Query: 170 GSRNFLDNLGFTDREEGDLGPVYGFQWR 253
+ N+ D ++G+LGPVYG QWR
Sbjct: 87 WTPNYEDQAISMGYDKGNLGPVYGKQWR 114
>UniRef50_Q7WP13 Cluster: Thymidylate synthase; n=43;
Proteobacteria|Rep: Thymidylate synthase - Bordetella
bronchiseptica (Alcaligenes bronchisepticus)
Length = 323
Score = 74.1 bits (174), Expect = 4e-12
Identities = 34/72 (47%), Positives = 48/72 (66%), Gaps = 2/72 (2%)
Frame = +1
Query: 298 QGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADG--KLSCLLY 471
+ +DQL+ +D+I +P DRR+L WN + +++MALPPCH L QF G ++S LY
Sbjct: 153 KAVDQLRQCLDTIHQSPGDRRILFHGWNWAQIEEMALPPCHLLYQFLPNAGTREISLCLY 212
Query: 472 QRSADMGLGVPF 507
RS D+GLG PF
Sbjct: 213 IRSNDVGLGTPF 224
Score = 51.2 bits (117), Expect = 3e-05
Identities = 28/85 (32%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTL 668
GL + + + L H++ +TGY+ F + GD HVY NH+ L+ QL REP P L
Sbjct: 219 GLGTPFNLTEGAALLHLVGRLTGYKPRWFSYFIGDAHVYENHLPMLREQLTREPYPAPQL 278
Query: 669 EFARKIESID-DFKYEDFIIKDYKP 740
+ +I KYE ++ +P
Sbjct: 279 VLSDRIPDFAVTGKYEPQWLEQVEP 303
Score = 49.2 bits (112), Expect = 1e-04
Identities = 29/83 (34%), Positives = 41/83 (49%), Gaps = 1/83 (1%)
Frame = +2
Query: 8 GAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFL 187
GA R+ L P +TTK++ + I E++ + + + A G +WD N + N
Sbjct: 31 GAALRFDLQQG-FPAVTTKKLAFKSAIGEMVGFLRATRSAAQFRALGCKVWDQNANEN-E 88
Query: 188 DNLGFTDRE-EGDLGPVYGFQWR 253
L RE DLGPVYG QWR
Sbjct: 89 QWLANPYREGPDDLGPVYGVQWR 111
>UniRef50_Q6WIC0 Cluster: DTMP (Thymidylate) synthase; n=1; Vibrio
phage KVP40|Rep: DTMP (Thymidylate) synthase -
Bacteriophage KVP40
Length = 300
Score = 69.7 bits (163), Expect = 8e-11
Identities = 30/84 (35%), Positives = 52/84 (61%), Gaps = 2/84 (2%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFAR--K 683
IASY+LL H++A +TG + GE I + GD H+Y +HI + QL+R P++ P ++
Sbjct: 213 IASYALLVHILAQLTGLKVGELIFSGGDVHIYEDHIVQVLKQLRRTPRELPQIQLPEFDT 272
Query: 684 IESIDDFKYEDFIIKDYKPHPKIE 755
+ + + DF++ DY P+P ++
Sbjct: 273 LSQLLTKRASDFVVYDYDPYPALK 296
Score = 52.4 bits (120), Expect = 1e-05
Identities = 32/76 (42%), Positives = 44/76 (57%), Gaps = 8/76 (10%)
Frame = +1
Query: 304 IDQLQSVIDSIKNNP---ADRRMLICAWN-----SSDLKKMALPPCHCLAQFYVADGKLS 459
I QL I ++K +P A RRM++ AW+ S+ +ALPPCH Q +V +GK+S
Sbjct: 136 IHQLLCDIAAVKEDPTQSAQRRMIVEAWDPYFHTESNSVDVALPPCHYGFQVFVRNGKIS 195
Query: 460 CLLYQRSADMGLGVPF 507
QRS D LG+PF
Sbjct: 196 LKWNQRSVDTFLGLPF 211
Score = 44.4 bits (100), Expect = 0.003
Identities = 30/76 (39%), Positives = 39/76 (51%), Gaps = 7/76 (9%)
Frame = +2
Query: 47 PLLTTKRVFTRGVIEELLWMISGS---TDSK---ALAAKGVHIWDAN-GSRNFLDNLGFT 205
PLLTTK++ T+ + ELLW +SGS +D + L+ + IW N N
Sbjct: 44 PLLTTKKMATKAIFGELLWFLSGSGLISDLRKYTELSDEDFCIWQQNLDELNERAKKAGI 103
Query: 206 DREEGDLGPVYGFQWR 253
R E DLG VYG WR
Sbjct: 104 VRNEDDLGYVYGKVWR 119
>UniRef50_Q2S5G7 Cluster: Thymidylate synthase; n=8; Bacteria|Rep:
Thymidylate synthase - Salinibacter ruber (strain DSM
13855)
Length = 355
Score = 69.3 bits (162), Expect = 1e-10
Identities = 35/81 (43%), Positives = 48/81 (59%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASY+LLT M+A G E I GD H+Y NH+E ++ QL R P PTL R+ +
Sbjct: 270 IASYALLTRMLAQQAGLTPHELIFNGGDCHIYQNHVEQVEEQLTRTPYDRPTLRL-RQRD 328
Query: 690 SIDDFKYEDFIIKDYKPHPKI 752
SI + +D I+DY+ HP +
Sbjct: 329 SIFEHAADDVTIEDYEHHPAL 349
Score = 64.9 bits (151), Expect = 2e-09
Identities = 41/100 (41%), Positives = 51/100 (51%), Gaps = 16/100 (16%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+FG R+ L PLLTTKRV+ RGV EELLW + G T+ ++L V IW +
Sbjct: 90 VFGRQLRFDLTEG-FPLLTTKRVWMRGVTEELLWFLRGETNIQSLVQADVSIWTDWPLQR 148
Query: 182 FLDNLG-------FTDR---------EEGDLGPVYGFQWR 253
+ D G F +R GDLGPVYG QWR
Sbjct: 149 YRDETGDEISQEAFEERIATDDAFAERWGDLGPVYGTQWR 188
Score = 61.7 bits (143), Expect = 2e-08
Identities = 27/72 (37%), Positives = 44/72 (61%), Gaps = 4/72 (5%)
Frame = +1
Query: 304 IDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYV----ADGKLSCLLY 471
+DQ++ ++ ++ P RR ++ AW+ + ++ ALPPCH Q +V DG+LS +
Sbjct: 197 VDQIERLVRGLRETPHSRRHVVSAWHPAQIEDAALPPCHYAFQCFVEGEPGDGRLSLMWQ 256
Query: 472 QRSADMGLGVPF 507
QRS D LG+PF
Sbjct: 257 QRSVDSFLGLPF 268
>UniRef50_Q934U8 Cluster: Putative thymidylate synthase; n=1;
Salmonella typhi|Rep: Putative thymidylate synthase -
Salmonella typhi
Length = 371
Score = 68.1 bits (159), Expect = 2e-10
Identities = 32/85 (37%), Positives = 51/85 (60%), Gaps = 2/85 (2%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREP--KQFPTLEFARK 683
IA Y +LTH +A +TG+ AG F+H D H+Y NH+ ++ ++REP + P + F +
Sbjct: 283 IAGYGILTHFLAKITGHMAGAFVHFGFDVHLYDNHMGGVEELMKREPPKESDPVVIFPHE 342
Query: 684 IESIDDFKYEDFIIKDYKPHPKIEM 758
+DDFK+E + Y P P I++
Sbjct: 343 WAELDDFKWEGVQVCGYSPLPWIKV 367
Score = 56.4 bits (130), Expect = 8e-07
Identities = 21/39 (53%), Positives = 30/39 (76%)
Frame = +1
Query: 304 IDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCH 420
IDQLQ ++ +++ NP DRR+L+ AWN +L+ M LPPCH
Sbjct: 163 IDQLQRIVHTLRTNPTDRRILLNAWNVGELEDMKLPPCH 201
Score = 51.2 bits (117), Expect = 3e-05
Identities = 30/77 (38%), Positives = 42/77 (54%)
Frame = +2
Query: 23 YSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLGF 202
Y L ++PL++ K V + ++ EL W + G+ + + L GV IWDA +
Sbjct: 55 YMLTGGSVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDA-----------W 103
Query: 203 TDREEGDLGPVYGFQWR 253
D E GDLGPVYG QWR
Sbjct: 104 AD-ENGDLGPVYGKQWR 119
>UniRef50_Q6KGF1 Cluster: Putative thymidylate synthase; n=1;
Enterobacteria phage Felix 01|Rep: Putative thymidylate
synthase - Enterobacteria phage Felix 01
Length = 299
Score = 67.3 bits (157), Expect = 4e-10
Identities = 31/76 (40%), Positives = 49/76 (64%), Gaps = 3/76 (3%)
Frame = +1
Query: 289 YTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDL--KKMALPPCHCLAQFYVA-DGKLS 459
+ G +DQL++++ +K + DR ML+ AWN++D+ MAL PCH + Q Y+ +G++
Sbjct: 129 HNGCVVDQLETLVTKMKGDITDRYMLVNAWNAADIAANSMALAPCHVMFQIYITNEGEVD 188
Query: 460 CLLYQRSADMGLGVPF 507
YQRS D LG+PF
Sbjct: 189 LQWYQRSVDTFLGLPF 204
Score = 50.4 bits (115), Expect = 5e-05
Identities = 29/87 (33%), Positives = 43/87 (49%), Gaps = 5/87 (5%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
IASY + ++ +TGY I GDT +Y NH++ + + E PT E +
Sbjct: 206 IASYGFILEVLCKMTGYTPRYLIGVFGDTQIYQNHMKQVYELMNNEEFHAPTFEIGLPLN 265
Query: 690 SIDDFKY---EDFI--IKDYKPHPKIE 755
++ D K+ DFI I +YK KIE
Sbjct: 266 TLSDLKHLTASDFIGGINNYKHAGKIE 292
Score = 39.5 bits (88), Expect = 0.099
Identities = 29/78 (37%), Positives = 37/78 (47%), Gaps = 9/78 (11%)
Frame = +2
Query: 47 PLLTTKRVFTRGVIEELLWMISGSTDSKALAAK--GVH------IWDANGSRNFLDNLGF 202
PLLT+K+VFTR VI E LW ++G L + G + IW + R N
Sbjct: 47 PLLTSKQVFTRQVIGEALWFLNGENKLSELRYRTWGENDGERWTIWSDDFKRWLSSNYSS 106
Query: 203 -TDREEGDLGPVYGFQWR 253
D E G +YG QWR
Sbjct: 107 EQDWLEDAGGRIYGVQWR 124
>UniRef50_Q3F0X9 Cluster: Thymidylate synthase; n=1; Bacillus
thuringiensis serovar israelensis ATCC 35646|Rep:
Thymidylate synthase - Bacillus thuringiensis serovar
israelensis ATCC 35646
Length = 286
Score = 66.5 bits (155), Expect = 8e-10
Identities = 28/84 (33%), Positives = 48/84 (57%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
+ Y++L HM+A G + GE +H GD H+Y H+E + ++REP PTL +
Sbjct: 200 VCEYAILLHMVARHIGQEVGELLHIIGDCHIYNKHLELAEELVKREPYAAPTLWINPEKT 259
Query: 690 SIDDFKYEDFIIKDYKPHPKIEME 761
F +DF +++Y HP++++E
Sbjct: 260 DFYSFTADDFRLENYDKHPQMKIE 283
Score = 46.8 bits (106), Expect = 7e-04
Identities = 23/60 (38%), Positives = 33/60 (55%)
Frame = +1
Query: 307 DQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSAD 486
+Q+ V+ +K +P RR +I WN D +M LPPC QF+ K + +L QRS D
Sbjct: 132 NQMDYVLGELKRDPFTRRAVIEMWNVDDTGRMNLPPCAHHLQFWSDGKKTNLILKQRSQD 191
>UniRef50_Q3IQP2 Cluster: Thymidylate synthase; n=4;
Halobacteriaceae|Rep: Thymidylate synthase -
Natronomonas pharaonis (strain DSM 2160 / ATCC 35678)
Length = 342
Score = 64.9 bits (151), Expect = 2e-09
Identities = 30/70 (42%), Positives = 43/70 (61%)
Frame = +1
Query: 298 QGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQR 477
Q DQL V+D+++ NP RR+++ AW+ ++ LPPCH F V +L+ L QR
Sbjct: 137 QVFDQLAYVLDTLEENPHSRRLVVNAWHPANAAVSTLPPCHYTFVFNVQGDQLNVHLTQR 196
Query: 478 SADMGLGVPF 507
S D+ LGVPF
Sbjct: 197 SGDIALGVPF 206
Score = 39.9 bits (89), Expect = 0.075
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVY 605
+A+YSLL H +A+ T ++ G F HT D H+Y
Sbjct: 208 LAAYSLLAHAVANRTAFEVGSFGHTIVDAHIY 239
Score = 39.9 bits (89), Expect = 0.075
Identities = 19/49 (38%), Positives = 28/49 (57%)
Frame = +3
Query: 606 LNHIEPLKIQLQREPKQFPTLEFARKIESIDDFKYEDFIIKDYKPHPKI 752
L+H+ L QL R P+ P +E A + +DD +Y+D + DY P P I
Sbjct: 290 LDHVPGLLTQLSRSPRDRPEIEVADR--PLDDLEYDDVQLHDYDPAPGI 336
Score = 33.1 bits (72), Expect = 8.6
Identities = 27/72 (37%), Positives = 34/72 (47%), Gaps = 3/72 (4%)
Frame = +2
Query: 47 PLLTTKRVFT---RGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLGFTDREE 217
PLLTTKR+ +I ELLW +SG + L + IWDA + D +E
Sbjct: 43 PLLTTKRMDGYRWNSLIHELLWYLSGEEHIRTLREE-TKIWDA-----------WAD-DE 89
Query: 218 GDLGPVYGFQWR 253
G L YG WR
Sbjct: 90 GHLDTAYGRFWR 101
>UniRef50_A5Z9U8 Cluster: Thymidylate synthase; n=9; Bacteria|Rep:
Thymidylate synthase - Eubacterium ventriosum ATCC 27560
Length = 282
Score = 60.1 bits (139), Expect = 7e-08
Identities = 27/82 (32%), Positives = 43/82 (52%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIE 689
+ Y+ L HM A V + GEFIH D H+Y H+ ++ + RE P L +I+
Sbjct: 195 VCQYAALIHMFAQVNDMEVGEFIHVIADAHIYDKHVPLIEELITREQHPAPKLWINPEIK 254
Query: 690 SIDDFKYEDFIIKDYKPHPKIE 755
+ DF +D ++DY P+I+
Sbjct: 255 NFYDFTVDDLKLEDYVTGPQIK 276
Score = 38.3 bits (85), Expect = 0.23
Identities = 24/73 (32%), Positives = 37/73 (50%), Gaps = 6/73 (8%)
Frame = +1
Query: 289 YTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGK----- 453
Y +DQ+ V+ +K+NP RR++ +N DL +M L PC F V +
Sbjct: 115 YKEGDMDQVDRVLYDLKHNPYSRRIMTNIYNHEDLHEMNLYPCAYSMTFNVTKNEETGNL 174
Query: 454 -LSCLLYQRSADM 489
L+ +L QRS D+
Sbjct: 175 VLNGILNQRSQDV 187
>UniRef50_Q8RGP6 Cluster: Thymidylate synthase; n=3; Fusobacterium
nucleatum|Rep: Thymidylate synthase - Fusobacterium
nucleatum subsp. nucleatum
Length = 275
Score = 53.2 bits (122), Expect = 8e-06
Identities = 27/64 (42%), Positives = 38/64 (59%)
Frame = +1
Query: 310 QLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSADM 489
QL VI+ +K NP RR++ W ++L +MAL PC L Q+ V KL + QRS D+
Sbjct: 123 QLHYVINELKKNPNSRRIMTEIWIPNELSEMALTPCVHLTQWSVIGNKLYLEVRQRSCDV 182
Query: 490 GLGV 501
LG+
Sbjct: 183 ALGL 186
Score = 44.4 bits (100), Expect = 0.003
Identities = 31/104 (29%), Positives = 47/104 (45%)
Frame = +3
Query: 450 EIILLVVPKKC*HGLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLK 629
++ L V + C L + YS+L ++A G + E I + H+Y H + L
Sbjct: 170 KLYLEVRQRSCDVALGLVANVFQYSVLHKLVALECGLEPAEIIWNIHNMHIYDRHYDKLI 229
Query: 630 IQLQREPKQFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEME 761
Q+ RE + P +SI DFK +D I DYK K+ E
Sbjct: 230 EQVNRETFE-PAKIKINNFKSIFDFKPDDIEIIDYKYGEKVSYE 272
>UniRef50_Q87SA0 Cluster: Thymidylate synthase; n=76;
Proteobacteria|Rep: Thymidylate synthase - Vibrio
parahaemolyticus
Length = 283
Score = 51.6 bits (118), Expect = 2e-05
Identities = 30/86 (34%), Positives = 45/86 (52%), Gaps = 2/86 (2%)
Frame = +2
Query: 2 IFGAMQRYSLHNNTLPLLTTKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRN 181
+ A Y + NN PL+TT++ F + + ELL I G +++ G WDAN + N
Sbjct: 30 VINADLTYDVANNQFPLVTTRKSFWKAAVAELLGYIRGYDNAEDFRKLGTKTWDANANLN 89
Query: 182 --FLDNLGFTDREEGDLGPVYGFQWR 253
+L+N + E D+G VYG Q R
Sbjct: 90 DAWLNNP--YRKGEDDMGRVYGVQGR 113
Score = 49.6 bits (113), Expect = 9e-05
Identities = 24/77 (31%), Positives = 46/77 (59%), Gaps = 5/77 (6%)
Frame = +3
Query: 537 MIAHVTGYQAGEFIHTTGDTHVYLNHIEPLK-IQLQREPKQFPTLEFARKIESIDDFK-- 707
++A +TG + G+ H + H+Y + +E ++ +QL+REP Q PT +I+S++D +
Sbjct: 201 IMAQITGKKPGQAFHKIVNAHIYEDQLELMRDVQLKREPLQAPTFHINPEIKSLEDLETW 260
Query: 708 --YEDFIIKDYKPHPKI 752
+DF ++ Y+ H I
Sbjct: 261 VTLDDFWVEGYEHHDPI 277
Score = 39.1 bits (87), Expect = 0.13
Identities = 22/71 (30%), Positives = 33/71 (46%)
Frame = +1
Query: 295 GQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQ 474
G IDQL+ ++D + DR ++ +N + L PC F + D L Q
Sbjct: 120 GGHIDQLRKIVDDLTRGVDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLDDTLYLNSTQ 179
Query: 475 RSADMGLGVPF 507
RS D+ LG+ F
Sbjct: 180 RSCDVPLGLNF 190
>UniRef50_A2SMX7 Cluster: Putative thymidylate synthase; n=1;
Methylibium petroleiphilum PM1|Rep: Putative thymidylate
synthase - Methylibium petroleiphilum (strain PM1)
Length = 316
Score = 49.6 bits (113), Expect = 9e-05
Identities = 24/68 (35%), Positives = 41/68 (60%), Gaps = 2/68 (2%)
Frame = +1
Query: 298 QGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHC--LAQFYVADGKLSCLLY 471
+ I+QL++ + ++ NP+DR +++ WN +++ + AL CH + Q VA G L L+
Sbjct: 150 RSINQLETSLHAVMTNPSDRGIILSGWNPAEIDQGALRSCHVSYMLQVDVATGTLHLTLW 209
Query: 472 QRSADMGL 495
QRS D L
Sbjct: 210 QRSFDAPL 217
Score = 41.9 bits (94), Expect = 0.019
Identities = 29/93 (31%), Positives = 43/93 (46%), Gaps = 9/93 (9%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQRE--PK------QFPT 665
+A ++ ++A + G +AG F H D H+Y HI +K L RE P+ PT
Sbjct: 221 VAIGAMYLEIMARLAGLKAGTFTHFISDAHIYAKHIPGVKEMLAREHFPQPTLDLGNIPT 280
Query: 666 LEFARKIESI-DDFKYEDFIIKDYKPHPKIEME 761
L A +I I + +Y+ HPKI E
Sbjct: 281 LTSAAEIPGIFATLDPNQVKLVNYQHHPKIAFE 313
>UniRef50_Q2FCV1 Cluster: Thymidylate synthase; n=1; Acinetobacter
baumannii|Rep: Thymidylate synthase - Acinetobacter
baumannii
Length = 222
Score = 41.9 bits (94), Expect = 0.019
Identities = 20/67 (29%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
Frame = +1
Query: 307 DQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALP--PCHCLAQFYVADGKLSCLLYQRS 480
+Q +I+ +K+ P RR +I ++ DL + PC CL QF++ + +L RS
Sbjct: 113 NQFNKIIEVLKDKPTSRRAVISIYSKDDLLREDSRDIPCTCLLQFFIRNERLHLTATMRS 172
Query: 481 ADMGLGV 501
D +G+
Sbjct: 173 NDAAIGL 179
>UniRef50_Q8DUI4 Cluster: Thymidylate synthase; n=45;
Streptococcaceae|Rep: Thymidylate synthase -
Streptococcus mutans
Length = 279
Score = 41.5 bits (93), Expect = 0.025
Identities = 27/85 (31%), Positives = 43/85 (50%), Gaps = 4/85 (4%)
Frame = +3
Query: 519 YSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREP---KQFPTLEFARKIE 689
Y L MIA G++ G+F + + H+Y N E + L+REP + L K
Sbjct: 193 YVALQMMIAKHFGWKVGKFFYFVNNLHIYDNQFEQAEELLRREPVVCQPHLVLNVPNK-T 251
Query: 690 SIDDFKYEDFIIKDYKP-HPKIEME 761
+ D K EDF + DY+P P+++ +
Sbjct: 252 NFFDIKPEDFELLDYEPVKPQLKFD 276
>UniRef50_Q8THH4 Cluster: Thymidylate synthase; n=6;
Methanosarcinales|Rep: Thymidylate synthase -
Methanosarcina acetivorans
Length = 217
Score = 41.1 bits (92), Expect = 0.033
Identities = 21/67 (31%), Positives = 31/67 (46%)
Frame = +1
Query: 304 IDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSA 483
+DQ++ VI+ ++ +P RR W K+ PC L F + DGK+ RS
Sbjct: 102 VDQIEYVIEKLQESPTSRRATAVTWIPPVDTKVNEVPCMILDDFKIRDGKVHLTTLFRSH 161
Query: 484 DMGLGVP 504
D G P
Sbjct: 162 DFGGAYP 168
>UniRef50_Q8VBA7 Cluster: Wsv068; n=2; Shrimp white spot syndrome
virus|Rep: Wsv068 - White spot syndrome virus (WSSV)
Length = 77
Score = 39.1 bits (87), Expect = 0.13
Identities = 24/52 (46%), Positives = 28/52 (53%)
Frame = -3
Query: 156 MCTPLAANAFESVDPEIIHSSSSITPLVNTRLVVRSGSVLL*REYLCIAPNI 1
MC AN+ SV P + H SSS TPL LVVR+G V RE L P +
Sbjct: 1 MCIFFLANSLASVLPLMNHKSSSTTPLQKIFLVVRTGIVSSRRENLIWGPKM 52
>UniRef50_A3RUA7 Cluster: Deoxyuridylate hydroxymethyltransferase;
n=2; Burkholderiaceae|Rep: Deoxyuridylate
hydroxymethyltransferase - Ralstonia solanacearum UW551
Length = 352
Score = 39.1 bits (87), Expect = 0.13
Identities = 24/74 (32%), Positives = 40/74 (54%), Gaps = 5/74 (6%)
Frame = +1
Query: 298 QGIDQLQSVIDSIKNNPADRRMLICAWNSSDLK-KMALP----PCHCLAQFYVADGKLSC 462
+G++Q+ +VI+ + N RR +I +N+ DL +A+ PC C QF + +L
Sbjct: 108 RGVNQVSNVIEKLSGNNDTRRAVIQLFNAEDLGCDVAVGRKDIPCTCTMQFSLRARQLHM 167
Query: 463 LLYQRSADMGLGVP 504
+ RS D LG+P
Sbjct: 168 FVTMRSNDAYLGLP 181
>UniRef50_O26868 Cluster: Thymidylate synthase; n=3;
Methanothermobacter|Rep: Thymidylate synthase -
Methanobacterium thermoautotrophicum
Length = 222
Score = 38.7 bits (86), Expect = 0.17
Identities = 20/63 (31%), Positives = 28/63 (44%)
Frame = +1
Query: 301 GIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRS 480
G+DQL I +KN RR + W+ + PC L F + DG+L RS
Sbjct: 108 GVDQLDEAIRRLKNCTESRRATMVTWDPPEDTASDEVPCMILVDFKIRDGRLFTTALWRS 167
Query: 481 ADM 489
D+
Sbjct: 168 HDI 170
>UniRef50_Q1YFG6 Cluster: Possible thymidylate synthase; n=1;
Aurantimonas sp. SI85-9A1|Rep: Possible thymidylate
synthase - Aurantimonas sp. SI85-9A1
Length = 338
Score = 37.1 bits (82), Expect = 0.53
Identities = 20/68 (29%), Positives = 34/68 (50%), Gaps = 1/68 (1%)
Frame = +1
Query: 304 IDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALP-PCHCLAQFYVADGKLSCLLYQRS 480
IDQ S+ ++ P RR ++ +N+ D+ PC QF++ +G+L + RS
Sbjct: 115 IDQFDSIERLLRRKPGSRRAVVQLFNAEDIATDHKEIPCTTTLQFHLREGRLHLSVTLRS 174
Query: 481 ADMGLGVP 504
D G+P
Sbjct: 175 NDAYWGLP 182
>UniRef50_A0ITX4 Cluster: Thymidylate synthase; n=1; Serratia
proteamaculans 568|Rep: Thymidylate synthase - Serratia
proteamaculans 568
Length = 325
Score = 36.7 bits (81), Expect = 0.70
Identities = 16/65 (24%), Positives = 34/65 (52%), Gaps = 1/65 (1%)
Frame = +3
Query: 510 IASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLK-IQLQREPKQFPTLEFARKI 686
+ ++++L ++A +TG + G + H H+Y H E +K +Q + P + +I
Sbjct: 189 VFAFTMLQELVARLTGCKLGHYHHFVSSLHIYEEHFEQVKALQQEGFMSTLPVMGDMPEI 248
Query: 687 ESIDD 701
+S+ D
Sbjct: 249 QSLKD 253
>UniRef50_P31654 Cluster: Deoxyuridylate hydroxymethyltransferase;
n=1; Bacillus phage SPO1|Rep: Deoxyuridylate
hydroxymethyltransferase - Bacteriophage SP01
Length = 383
Score = 36.7 bits (81), Expect = 0.70
Identities = 23/71 (32%), Positives = 37/71 (52%), Gaps = 6/71 (8%)
Frame = +1
Query: 304 IDQLQSVIDSIKNNPADRRMLICAWN------SSDLKKMALPPCHCLAQFYVADGKLSCL 465
+DQL+ V + IK +P R+ + +N ++D K PC+ L F + +GKL
Sbjct: 122 LDQLRDVYEKIKADPDTRQAVAVIYNPLFDNINNDTKDR---PCNLLLSFKLRNGKLDLS 178
Query: 466 LYQRSADMGLG 498
+Y RS D+ G
Sbjct: 179 VYNRSNDLHWG 189
>UniRef50_Q1VC99 Cluster: Putative uncharacterized protein; n=1;
Vibrio alginolyticus 12G01|Rep: Putative uncharacterized
protein - Vibrio alginolyticus 12G01
Length = 142
Score = 36.3 bits (80), Expect = 0.93
Identities = 22/78 (28%), Positives = 39/78 (50%)
Frame = +3
Query: 537 MIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIESIDDFKYED 716
MI + Y A EF+ + + Y++H E K +L++ KQF + +ES++ K D
Sbjct: 49 MIDENSSYVAVEFVGKWEEINNYVSHKEAKKRKLEKVNKQFVYSQVGETLESLEQ-KVVD 107
Query: 717 FIIKDYKPHPKIEMERQY 770
I D + +++ R Y
Sbjct: 108 HIYADQPKYFSVDVYRDY 125
>UniRef50_Q21ZJ9 Cluster: Thymidylate synthase; n=1; Rhodoferax
ferrireducens T118|Rep: Thymidylate synthase -
Rhodoferax ferrireducens (strain DSM 15236 / ATCC
BAA-621 / T118)
Length = 347
Score = 35.5 bits (78), Expect = 1.6
Identities = 24/75 (32%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
Frame = +1
Query: 301 GIDQLQSVIDSIKNNPADRRMLICAWNS------SDLKKMALP-PCHCLAQFYVADGKLS 459
G+DQ++ V + NP R+ ++ W+S SD L PC+ + V KL
Sbjct: 113 GLDQIERVYQILSANPESRQAVLQIWDSREDLPLSDGASRDLDIPCNLVGMLKVRAQKLE 172
Query: 460 CLLYQRSADMGLGVP 504
L RS D+ LG P
Sbjct: 173 WLQIMRSNDVFLGTP 187
>UniRef50_Q86B02 Cluster: Similar to Dictyostelium discoideum (Slime
mold). Nucleotide exchange factor RasGEF P; n=3;
Eukaryota|Rep: Similar to Dictyostelium discoideum (Slime
mold). Nucleotide exchange factor RasGEF P -
Dictyostelium discoideum (Slime mold)
Length = 2454
Score = 33.9 bits (74), Expect = 4.9
Identities = 19/68 (27%), Positives = 33/68 (48%)
Frame = +3
Query: 498 SSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFA 677
S +YI+S++ + + YQ I+ TG + + +H E L +QL +P Q T +
Sbjct: 2136 SFMYISSFNPSMNKNMLINEYQITHIINCTGSPNAFPDHFEYLHLQLHDQPHQDITQSLS 2195
Query: 678 RKIESIDD 701
+ I D
Sbjct: 2196 LAFDFIRD 2203
>UniRef50_A7TG56 Cluster: Putative uncharacterized protein; n=1;
Vanderwaltozyma polyspora DSM 70294|Rep: Putative
uncharacterized protein - Vanderwaltozyma polyspora DSM
70294
Length = 877
Score = 33.9 bits (74), Expect = 4.9
Identities = 23/59 (38%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
Frame = +2
Query: 26 SLHNNTLPLLTTKRVFTRG--VIEELLWMISGSTDSKALAAKGVHIWDANGSRNF-LDN 193
+LH T+P++ K T IEEL M G T+ +A+ A H WD + + NF LDN
Sbjct: 820 TLHTPTVPVIDRKLATTPKSLAIEELCGM--GFTEDEAVKALEKHNWDLDAATNFLLDN 876
>UniRef50_UPI00015972C9 Cluster: hypothetical protein RBAM_005690;
n=1; Bacillus amyloliquefaciens FZB42|Rep: hypothetical
protein RBAM_005690 - Bacillus amyloliquefaciens FZB42
Length = 336
Score = 33.5 bits (73), Expect = 6.5
Identities = 26/99 (26%), Positives = 45/99 (45%), Gaps = 8/99 (8%)
Frame = +3
Query: 489 GLRSSIYIASYSLLTHMIAHVTGYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPK--QFP 662
G+ S ++ S++ + ++A + GE+ H G H+Y + E K L+ P
Sbjct: 180 GMISDLF--SFTFMQELMARELRLEVGEYFHNAGSIHIYDSDHEWAKTVLEEAENHHDIP 237
Query: 663 TLEFARKIE-----SIDD-FKYEDFIIKDYKPHPKIEME 761
EF + E SI+ KYE+ + KD K ++E
Sbjct: 238 EFEFPKMPEGNNWPSIETVLKYEELLRKDQISMTKNDIE 276
>UniRef50_Q4C4J0 Cluster: Thymidylate synthase; n=1; Crocosphaera
watsonii WH 8501|Rep: Thymidylate synthase -
Crocosphaera watsonii
Length = 502
Score = 33.5 bits (73), Expect = 6.5
Identities = 21/80 (26%), Positives = 35/80 (43%)
Frame = +1
Query: 250 ETSGAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKKMALPPCHCLA 429
+ G KY Q + DQ++ ID + ++ R+++ W+ D + PPC
Sbjct: 269 QKEGVKYTYGQRLRSWFERDQIKQAIDKLISDKDSSRVVMSLWDVKDYENNDSPPCLNHI 328
Query: 430 QFYVADGKLSCLLYQRSADM 489
+ D +LS RS DM
Sbjct: 329 WVRIVDDELSLTATFRSNDM 348
>UniRef50_A7TUR1 Cluster: Putative thymidylate synthase; n=1;
Streptomyces lividans|Rep: Putative thymidylate synthase
- Streptomyces lividans
Length = 308
Score = 33.1 bits (72), Expect = 8.6
Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
Frame = +1
Query: 295 GQGIDQLQSVIDSIKNNPADRRMLICAWN-SSDLKKMALPPCHCLAQFYVADGKLSCLLY 471
G +DQL V++ ++ + RR LI ++ + D PC +F++ G+L
Sbjct: 100 GGKVDQLARVVEILQADSDSRRALIQLYDPAQDAAGHKDVPCTLGFRFHLRAGRLHMATM 159
Query: 472 QRSADMGLGVPF 507
R D+ +G+P+
Sbjct: 160 MRGQDVWIGMPY 171
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 819,141,555
Number of Sequences: 1657284
Number of extensions: 16531265
Number of successful extensions: 42645
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 40920
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42591
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 70914189703
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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