Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0898
(743 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g47490.1 68418.m05864 expressed protein 34 0.087
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 32 0.46
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 32 0.46
At3g24480.1 68416.m03070 leucine-rich repeat family protein / ex... 30 1.9
At2g27470.1 68415.m03320 CCAAT-box binding transcription factor ... 29 4.3
At5g40300.1 68418.m04888 integral membrane protein, putative MtN... 28 5.7
At5g08440.1 68418.m00994 expressed protein 28 5.7
At4g32375.1 68417.m04610 glycoside hydrolase family 28 protein /... 28 5.7
At4g13390.1 68417.m02092 proline-rich extensin-like family prote... 28 5.7
At5g61940.1 68418.m07775 ubiquitin carboxyl-terminal hydrolase-r... 28 7.5
At5g13590.1 68418.m01572 expressed protein 28 7.5
At1g47900.1 68414.m05334 expressed protein 28 7.5
At1g06190.1 68414.m00651 expressed protein 28 7.5
At2g41850.1 68415.m05172 endo-polygalacturonase, putative simila... 27 9.9
>At5g47490.1 68418.m05864 expressed protein
Length = 1361
Score = 34.3 bits (75), Expect = 0.087
Identities = 20/39 (51%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +2
Query: 431 GQTRSRAR-VQVR*PRTHGILDSTIPSPTVHYAKPPIPA 544
G+T RAR V P G + IPSP+V AKPPIPA
Sbjct: 1203 GRTGVRARYVDTYNPPGRGNSHTMIPSPSVQTAKPPIPA 1241
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 31.9 bits (69), Expect = 0.46
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +2
Query: 503 PSPTVHYAKPPIPANGIATRPPERDGPFV 589
P+PT HY PP P I + PP+ P +
Sbjct: 726 PTPTYHYISPPPPPTPIHSPPPQSHPPCI 754
Score = 29.5 bits (63), Expect = 2.5
Identities = 16/39 (41%), Positives = 20/39 (51%)
Frame = +2
Query: 503 PSPTVHYAKPPIPANGIATRPPERDGPFVFGIHSPSQFP 619
P PTVHY PP P+ + PP P V+ +SP P
Sbjct: 760 PPPTVHYNPPPPPSPAHYSPPP---SPPVYYYNSPPPPP 795
Score = 27.5 bits (58), Expect = 9.9
Identities = 18/49 (36%), Positives = 23/49 (46%), Gaps = 4/49 (8%)
Frame = +2
Query: 491 DSTIPSPTVHYAKPPIPA--NGIATRPPERDGPF--VFGIHSPSQFPIP 625
+S P P VHY+ PP P + PP +GP + GI S P P
Sbjct: 789 NSPPPPPAVHYSPPPPPVIHHSQPPPPPIYEGPLPPIPGISYASPPPPP 837
>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-1) nearly identical to
starch branching enzyme class II [Arabidopsis thaliana]
GI:619939
Length = 858
Score = 31.9 bits (69), Expect = 0.46
Identities = 25/69 (36%), Positives = 36/69 (52%), Gaps = 2/69 (2%)
Frame = +2
Query: 449 ARVQVR*PRTHGILDSTIPSPTVHYAKPP--IPANGIATRPPERDGPFVFGIHSPSQFPI 622
+RV++R GI DS IP+ + +PP IP NG+ PPE D + F + P
Sbjct: 268 SRVKIRMDTPSGIKDS-IPAWIKYSVQPPGEIPYNGVYYDPPEED-KYAF------KHPR 319
Query: 623 PEKTTMITI 649
P+K T + I
Sbjct: 320 PKKPTSLRI 328
>At3g24480.1 68416.m03070 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 494
Score = 29.9 bits (64), Expect = 1.9
Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 5/46 (10%)
Frame = +2
Query: 503 PSPTVHYAKPPIPANGIATRP---PERDGPF--VFGIHSPSQFPIP 625
P P++HY+ PP P ++ P PE +GP V G+ S P P
Sbjct: 447 PPPSIHYSSPPPPPVHHSSPPPPSPEFEGPLPPVIGVSYASPPPPP 492
>At2g27470.1 68415.m03320 CCAAT-box binding transcription factor
subunit HAP3-related contains Pfam PF00808 :
Histone-like transcription factor (CBF/NF-Y) and
archaeal histone; similar to polymerase epsilon p17
subunit (DNA polymerase epsilon subunit 3) (YB-like
protein 1) (YBL1) (NF-YB-like protein) (SP:Q9JKP7) [Mus
musculus];
Length = 275
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +1
Query: 631 DDDDYDYSEVTEENGRTVIRDDDSEDLNCCDKVND 735
D++D + E TEENG DD++ + N D+ N+
Sbjct: 184 DEEDENDDENTEENGNDEENDDENTEENGNDEENE 218
>At5g40300.1 68418.m04888 integral membrane protein, putative MtN24
gene, Medicago truncatula, EMBL:MTY15290; contains Pfam
PF04535 : Domain of unknown function (DUF588); contains
4 transmembrane domains; similar to putative ethylene
responsive element binding protein (GI:22135858)
[Arabidopsis thaliana]
Length = 270
Score = 28.3 bits (60), Expect = 5.7
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = +3
Query: 273 PHRPLPAPRNLPDYRTDYRKPSNVELEKRLQESRAREKISYFQDKVTPPE 422
PH +P P ++ T +R +N E EK++ ESR + + S+ TP E
Sbjct: 65 PHTIIPTP-SVAKTETPFRV-TNGEEEKKVSESRRQLRPSFSSSSSTPRE 112
>At5g08440.1 68418.m00994 expressed protein
Length = 726
Score = 28.3 bits (60), Expect = 5.7
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -2
Query: 490 EYSVGSWLSNLNTSSASRLSKFNSGGVTL 404
EY + W S++N S+AS LS + GV L
Sbjct: 356 EYQLAPWQSDVNHSNASPLSPYQPVGVGL 384
>At4g32375.1 68417.m04610 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:4325090;
contains Pfam profile PF00295: Polygalacturonase
(pectinase)
Length = 486
Score = 28.3 bits (60), Expect = 5.7
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +3
Query: 252 RPPRDIGPHRPLPAPRNLPDYRTDYRKPSNVEL 350
+PPR +GP P P P + P +VEL
Sbjct: 386 KPPRHVGPLMPTKPPTMFPKPLAPAKSPRHVEL 418
>At4g13390.1 68417.m02092 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 429
Score = 28.3 bits (60), Expect = 5.7
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = +2
Query: 503 PSPTVHYAKPPIPANGIATRPPERDGPF 586
PSPTV+Y PP P + PP PF
Sbjct: 357 PSPTVNYKSPPPPYVYNSPPPPPYYSPF 384
>At5g61940.1 68418.m07775 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of
unknown function (DUF627)
Length = 1094
Score = 27.9 bits (59), Expect = 7.5
Identities = 14/45 (31%), Positives = 22/45 (48%), Gaps = 6/45 (13%)
Frame = -1
Query: 602 CVFRKQKVRRVPEDEW------LSHLLESEVSHNALSGSVLCCRV 486
CV ++ R +PED+W L + E V N+L+ + C V
Sbjct: 564 CVRENERRRNLPEDQWSRYASLLCDVCEERVPENSLTTKLFVCAV 608
>At5g13590.1 68418.m01572 expressed protein
Length = 1190
Score = 27.9 bits (59), Expect = 7.5
Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 2/62 (3%)
Frame = +3
Query: 303 LPDYRTDYRKPSNVELEKRLQESRAR--EKISYFQDKVTPPELNLDRREAELVFRFDNHE 476
+PD R R P N E Q+++++ K Y + K+ ++ D F FDNH
Sbjct: 786 IPDRR---RLPDNTESNLHDQDTKSKVLSKFFYGKTKLIVSKIQYDSHLVNAEFEFDNHG 842
Query: 477 PT 482
T
Sbjct: 843 NT 844
>At1g47900.1 68414.m05334 expressed protein
Length = 1054
Score = 27.9 bits (59), Expect = 7.5
Identities = 23/91 (25%), Positives = 35/91 (38%), Gaps = 3/91 (3%)
Frame = +1
Query: 436 DEKQSSCSGSITTNPRNTRQHNTEPDSAL---CETXXXXXXXXXXXXGTRRTFCFRNTQS 606
++ SCSGS++TNP ++Q E D A E+ + C N S
Sbjct: 483 NDDSGSCSGSLSTNP--SQQIKKEKDMAALERVESVNSHVELMDDFLEMEKLACLPNLSS 540
Query: 607 ESVPHPRKDDDDYDYSEVTEENGRTVIRDDD 699
+ KD SE+ + T + D D
Sbjct: 541 SNGSIDSKDGSGDQKSEMVILDAHTDLEDSD 571
>At1g06190.1 68414.m00651 expressed protein
Length = 401
Score = 27.9 bits (59), Expect = 7.5
Identities = 11/41 (26%), Positives = 24/41 (58%)
Frame = +1
Query: 619 HPRKDDDDYDYSEVTEENGRTVIRDDDSEDLNCCDKVNDWA 741
+ ++DDD + +++++G + DD E L+ D+ +D A
Sbjct: 316 YQEEEDDDVTFDVLSQDDGILDVLSDDDESLDDADEDSDEA 356
>At2g41850.1 68415.m05172 endo-polygalacturonase, putative similar
to endo-polygalacturonase [Arabidopsis thaliana]
GI:2597824; contains PF00295: Glycosyl hydrolases family
28 (polygalacturonases)
Length = 433
Score = 27.5 bits (58), Expect = 9.9
Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +1
Query: 568 GTRRTFCFRNTQSESVPHPRKDDDDY-DYSEVTEENGRTVIRDDDSEDLN 714
GT F+N Q ++V +P D DY D S+ T E +++ D++
Sbjct: 330 GTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQVKNVVYRDIS 379
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,224,489
Number of Sequences: 28952
Number of extensions: 367006
Number of successful extensions: 1867
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1394
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1845
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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