Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0876
(489 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 146 1e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 83 3e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 70 2e-13
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 64 8e-12
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.5
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 146 bits (355), Expect = 1e-36
Identities = 72/145 (49%), Positives = 83/145 (57%)
Frame = +2
Query: 53 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 232
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 233 PRAILVDWSPAPWTLSALDLXXXXXXXXXXXXXXXXXXXXGPRDTTRXXXXXXXXXXXXX 412
PRA+LVD P +
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 413 XKEAESCDCLQGFQLTHSLGGGTGS 487
+EAE+CD LQGFQLTHSLGGGTGS
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGS 145
Score = 86.2 bits (204), Expect = 2e-18
Identities = 36/45 (80%), Positives = 40/45 (88%)
Frame = +1
Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVXGQSGAGNNWAKGHYTEGAEL 387
LEPGTMD+V+SG FG +FRPDN + GQSGAGN WAKGHYTEGAEL
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAEL 112
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 82.6 bits (195), Expect = 3e-17
Identities = 46/145 (31%), Positives = 68/145 (46%), Gaps = 1/145 (0%)
Frame = +2
Query: 56 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 235
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 236 RAILVDWSPAPWTLSALDLXXXXXXXXXXXXXXXXXXXXGP-RDTTRXXXXXXXXXXXXX 412
RAIL+D P D +
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERIFEDIMDMI 122
Query: 413 XKEAESCDCLQGFQLTHSLGGGTGS 487
+EA+ D L+GF L HS+ GGTGS
Sbjct: 123 DREADGSDSLEGFSLLHSIAGGTGS 147
Score = 39.1 bits (87), Expect = 4e-04
Identities = 15/40 (37%), Positives = 27/40 (67%), Gaps = 2/40 (5%)
Frame = +1
Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVXGQS--GAGNNWAKGH 366
LEP ++++ S +G ++ P+N + ++ GAGNNWA G+
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGY 108
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 70.1 bits (164), Expect = 2e-13
Identities = 46/147 (31%), Positives = 60/147 (40%), Gaps = 2/147 (1%)
Frame = +2
Query: 53 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 226
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 227 YVPRAILVDWSPAPWTLSALDLXXXXXXXXXXXXXXXXXXXXGPRDTTRXXXXXXXXXXX 406
YVPR+I VD P R
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 407 XXXKEAESCDCLQGFQLTHSLGGGTGS 487
+ A++C LQGF + HS GGGTGS
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGS 147
Score = 58.0 bits (134), Expect = 7e-10
Identities = 22/45 (48%), Positives = 31/45 (68%)
Frame = +1
Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVXGQSGAGNNWAKGHYTEGAEL 387
LEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G EL
Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKEL 114
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 64.5 bits (150), Expect = 8e-12
Identities = 43/151 (28%), Positives = 59/151 (39%), Gaps = 6/151 (3%)
Frame = +2
Query: 53 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 214
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 215 SGGKYVPRAILVDWSPAPWTLSALDLXXXXXXXXXXXXXXXXXXXXGPRDTTRXXXXXXX 394
GK+VPR+I VD P R
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 395 XXXXXXXKEAESCDCLQGFQLTHSLGGGTGS 487
+ A++C LQGF + HS GGGTGS
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGS 151
Score = 57.6 bits (133), Expect = 9e-10
Identities = 22/45 (48%), Positives = 31/45 (68%)
Frame = +1
Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVXGQSGAGNNWAKGHYTEGAEL 387
LEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E+
Sbjct: 74 LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.5
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 92 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 193
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,769,310
Number of Sequences: 5004
Number of extensions: 30931
Number of successful extensions: 85
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 73
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 82
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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