Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0868
(657 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 117 1e-27
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 64 2e-11
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 61 2e-10
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 53 3e-08
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 29 0.78
SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces ... 27 3.1
SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 3.1
SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 27 3.1
SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 26 4.2
SPBC21C3.08c |||ornithine aminotransferase|Schizosaccharomyces p... 25 9.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 117 bits (282), Expect = 1e-27
Identities = 55/87 (63%), Positives = 60/87 (68%)
Frame = +1
Query: 262 DNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKECENCDCLQGFQXXXXXXXXXXXXX 441
DN ++GQSGAGN WAKGHYTEGAEL DAVLDVVR+E E CD LQGFQ
Sbjct: 88 DNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGM 147
Query: 442 XXXXXXKIREEYPDRIMNTYSVAPRPK 522
KIREEYPDR+M T+SVAP PK
Sbjct: 148 GTLLLSKIREEYPDRMMATFSVAPAPK 174
Score = 97.9 bits (233), Expect = 1e-21
Identities = 43/71 (60%), Positives = 56/71 (78%)
Frame = +2
Query: 41 FWEIISEEHGIDPTGVYRGTSDLQLERISVYYNEASVATAESGGKYVPRAILLDLEPGTM 220
FW I++EHG+D G+Y GTS+ Q ER++VY+NEA+ GGKYVPRA+L+DLEPGTM
Sbjct: 20 FWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAA------GGKYVPRAVLVDLEPGTM 73
Query: 221 DAVRSGAYGQL 253
DAV+SG +G L
Sbjct: 74 DAVKSGKFGNL 84
Score = 78.6 bits (185), Expect = 7e-16
Identities = 35/48 (72%), Positives = 41/48 (85%)
Frame = +3
Query: 507 SPSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEALYDICYRTLKV 650
+P+PK SDTVVEPYNA LS+HQLVEN+DET+CIDNEAL I TLK+
Sbjct: 170 APAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 64.1 bits (149), Expect = 2e-11
Identities = 26/47 (55%), Positives = 37/47 (78%)
Frame = +3
Query: 510 PSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEALYDICYRTLKV 650
P+P+VS +VVEPYN+VL+ H ++N+D T+ +DNEA YDIC R L +
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDI 223
Score = 60.9 bits (141), Expect = 2e-10
Identities = 29/90 (32%), Positives = 45/90 (50%)
Frame = +1
Query: 262 DNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKECENCDCLQGFQXXXXXXXXXXXXX 441
+ V G+ A NN+A+GHYT G E++D+VL+ +R+ +NC LQGF
Sbjct: 94 EQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGL 153
Query: 442 XXXXXXKIREEYPDRIMNTYSVAPRPKYQT 531
++ EY + +SV P P+ T
Sbjct: 154 GALLLERLNMEYGKKSNLQFSVYPAPQVST 183
Score = 54.0 bits (124), Expect = 2e-08
Identities = 29/72 (40%), Positives = 43/72 (59%), Gaps = 2/72 (2%)
Frame = +2
Query: 44 WEIISEEHGIDPTGVYRGTSDLQLERISVYYNEA-SVATAESG-GKYVPRAILLDLEPGT 217
WE+ EHGI P G T + ++ + + Y N+ +E+G GK+VPR+I +DLEP
Sbjct: 21 WELYCLEHGIGPDGF--PTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNV 78
Query: 218 MDAVRSGAYGQL 253
+D VR+G Y L
Sbjct: 79 IDQVRTGPYKDL 90
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 60.9 bits (141), Expect = 2e-10
Identities = 30/90 (33%), Positives = 43/90 (47%)
Frame = +1
Query: 262 DNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKECENCDCLQGFQXXXXXXXXXXXXX 441
+ + G+ A NN+A+GHYT G ELVD V D +R+ +NC LQGF
Sbjct: 90 EQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGF 149
Query: 442 XXXXXXKIREEYPDRIMNTYSVAPRPKYQT 531
++ EY + +SV P P+ T
Sbjct: 150 GALLLERLAMEYTKKSKLQFSVYPAPQVST 179
Score = 58.8 bits (136), Expect = 6e-10
Identities = 24/47 (51%), Positives = 35/47 (74%)
Frame = +3
Query: 510 PSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEALYDICYRTLKV 650
P+P+VS +VVEPYN+VL+ H ++ D T+ +DNE+ YDIC R L +
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDI 219
Score = 49.2 bits (112), Expect = 5e-07
Identities = 28/72 (38%), Positives = 37/72 (51%), Gaps = 2/72 (2%)
Frame = +2
Query: 44 WEIISEEHGIDPTGVYRGTSDLQLER--ISVYYNEASVATAESGGKYVPRAILLDLEPGT 217
WE+ EHGI P G + Q S +++E GKYVPR+I +DLEP
Sbjct: 21 WELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQ------GKYVPRSIYVDLEPNV 74
Query: 218 MDAVRSGAYGQL 253
+D VR+G Y L
Sbjct: 75 IDQVRTGPYRDL 86
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 53.2 bits (122), Expect = 3e-08
Identities = 23/76 (30%), Positives = 38/76 (50%)
Frame = +1
Query: 286 GAGNNWAKGHYTEGAELVDAVLDVVRKECENCDCLQGFQXXXXXXXXXXXXXXXXXXXKI 465
GAGNNWA G Y+ + + ++D++ +E + D L+GF ++
Sbjct: 99 GAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERL 157
Query: 466 REEYPDRIMNTYSVAP 513
+ YP +I+ TYSV P
Sbjct: 158 NDRYPKKIIQTYSVFP 173
Score = 48.8 bits (111), Expect = 7e-07
Identities = 24/71 (33%), Positives = 39/71 (54%)
Frame = +2
Query: 41 FWEIISEEHGIDPTGVYRGTSDLQLERISVYYNEASVATAESGGKYVPRAILLDLEPGTM 220
FW+ + EHGI P G + ++R V++ ++ +Y+PRAIL+DLEP +
Sbjct: 21 FWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDT------RYIPRAILIDLEPRVV 74
Query: 221 DAVRSGAYGQL 253
+ + S YG L
Sbjct: 75 NNILSDTYGSL 85
Score = 41.1 bits (92), Expect = 1e-04
Identities = 20/48 (41%), Positives = 27/48 (56%)
Frame = +3
Query: 513 SPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEALYDICYRTLKVPN 656
S VSD VV+PYN++L++ +L N D +DN AL I L N
Sbjct: 175 SQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQN 222
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 28.7 bits (61), Expect = 0.78
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +1
Query: 304 AKGHYTEGAELVDAVLDVVRKECENCDCLQ 393
A+GH G ELV A D +RK+ EN L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 26.6 bits (56), Expect = 3.1
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +2
Query: 17 ISYSSSYNFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNE 142
+SY+ S E+ S HG D T ++ LER+ +E
Sbjct: 63 VSYAKSRTRLELTSSSHGSDTRSFNDKTKNVHLERVEKIASE 104
>SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 409
Score = 26.6 bits (56), Expect = 3.1
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = +2
Query: 20 SYSSSYNFWEIISEEHGIDPTGVYR 94
SY+ +Y FW + E G+ VY+
Sbjct: 361 SYTKAYQFWHNLREPEGVPAEEVYK 385
>SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1021
Score = 26.6 bits (56), Expect = 3.1
Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 6/55 (10%)
Frame = +3
Query: 507 SPSPKVSDTVVE------PYNAVLSIHQLVENTDETYCIDNEALYDICYRTLKVP 653
+P+P+ DTV+ PYNA+ +Q V+ T + A I Y K+P
Sbjct: 926 TPNPQQQDTVINAVPTFAPYNAMTKFNQKVKVMPGTGKVGKAARESIAYFMKKLP 980
>SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 622
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 95 GTSDLQLERISVYYNEASVATAESGGKYVPRAILLDLE 208
G+SD+ + +S + EAS + S VP+ +L D +
Sbjct: 49 GSSDVTMTSVSGHLTEASFPSEYSSWSSVPQDVLFDAQ 86
>SPBC21C3.08c |||ornithine aminotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 438
Score = 25.0 bits (52), Expect = 9.6
Identities = 20/59 (33%), Positives = 27/59 (45%), Gaps = 6/59 (10%)
Frame = +2
Query: 80 TGVYRGTSDLQLERISVYYNEASVATAESGGKYV------PRAILLDLEPGTMDAVRSG 238
TGV R L +E +V + + A SGG Y R I+L+ EPGT + G
Sbjct: 250 TGVARTGKMLCIEHSNVKPDVVILGKAISGGVYPVSAVLSSREIMLNFEPGTHGSTYGG 308
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,150,938
Number of Sequences: 5004
Number of extensions: 37008
Number of successful extensions: 138
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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