Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0838
         (748 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q29R09 Cluster: LD28546p; n=7; Coelomata|Rep: LD28546p ...   125   1e-27
UniRef50_Q86M58 Cluster: Ubiquitin conjugating enzyme UBC-25; n=...    87   6e-16
UniRef50_UPI0000F2BDCC Cluster: PREDICTED: similar to ubiquitin-...    85   2e-15
UniRef50_Q7Z7E8 Cluster: Ubiquitin-conjugating enzyme E2 Q1; n=6...    85   2e-15
UniRef50_Q4S862 Cluster: Chromosome 5 SCAF14709, whole genome sh...    80   7e-14
UniRef50_Q4S863 Cluster: Chromosome 5 SCAF14709, whole genome sh...    75   2e-12
UniRef50_UPI0000E1FDC5 Cluster: PREDICTED: similar to ubiquitin-...    46   0.001
UniRef50_Q54KZ9 Cluster: Putative uncharacterized protein; n=1; ...    34   4.3  
UniRef50_Q2UPM5 Cluster: Predicted membrane proteins; n=16; Pezi...    33   7.5  
UniRef50_Q5L4T9 Cluster: Putative uncharacterized protein; n=7; ...    33   9.9  

>UniRef50_Q29R09 Cluster: LD28546p; n=7; Coelomata|Rep: LD28546p -
           Drosophila melanogaster (Fruit fly)
          Length = 397

 Score =  125 bits (302), Expect = 1e-27
 Identities = 52/65 (80%), Positives = 62/65 (95%)
 Frame = +2

Query: 59  MACLNTLKLEIKTLEQVFPKNHERFQIMSASVDELTCRFVGKNGKKYEIHANITETYPTT 238
           MACLNTLK EIKTLE++FPKNHERFQI+++SVDEL CRF+ KNGK+Y+IHANITETYP++
Sbjct: 1   MACLNTLKQEIKTLEKIFPKNHERFQILNSSVDELLCRFIDKNGKRYDIHANITETYPSS 60

Query: 239 PPVWF 253
           PPVWF
Sbjct: 61  PPVWF 65



 Score =  113 bits (273), Expect = 3e-24
 Identities = 55/71 (77%), Positives = 60/71 (84%)
 Frame = +3

Query: 534 LPLEMVEDAGRSNKDDMETEHLATLERLRQNQRPDYLSGSVSGSLQATDRLMKELRDIYR 713
           LPLEM +    S KDDME EHLATLE+LRQ+QR DYL GSVSGS+QATDRLMKELRDIYR
Sbjct: 177 LPLEMDDVRSTSKKDDMEVEHLATLEKLRQSQRQDYLKGSVSGSVQATDRLMKELRDIYR 236

Query: 714 SHSFKNNMYSI 746
           S +FK NMYSI
Sbjct: 237 SDAFKKNMYSI 247



 Score = 77.0 bits (181), Expect = 5e-13
 Identities = 35/42 (83%), Positives = 38/42 (90%)
 Frame = +1

Query: 256 ESEDPIVTNAVQILTNTQGRDNHVINQVGILLRELCKLHGVP 381
           ESE+  VTNAVQIL+NT GRDNHVINQVGILLRELC+LH VP
Sbjct: 67  ESEETSVTNAVQILSNTNGRDNHVINQVGILLRELCRLHNVP 108


>UniRef50_Q86M58 Cluster: Ubiquitin conjugating enzyme UBC-25; n=3;
           Caenorhabditis|Rep: Ubiquitin conjugating enzyme UBC-25
           - Caenorhabditis elegans
          Length = 387

 Score = 86.6 bits (205), Expect = 6e-16
 Identities = 37/67 (55%), Positives = 48/67 (71%)
 Frame = +2

Query: 59  MACLNTLKLEIKTLEQVFPKNHERFQIMSASVDELTCRFVGKNGKKYEIHANITETYPTT 238
           MACL  LK +I+ LE++FPKNH RFQI+SASVDEL+ +F+    K   + ANI E YP  
Sbjct: 1   MACLRKLKEDIQVLEKLFPKNHNRFQILSASVDELSMKFINAENKGIIVTANIQENYPRQ 60

Query: 239 PPVWFEK 259
           PP+WF +
Sbjct: 61  PPIWFSE 67



 Score = 59.3 bits (137), Expect = 1e-07
 Identities = 30/69 (43%), Positives = 43/69 (62%), Gaps = 1/69 (1%)
 Frame = +3

Query: 540 LEMVEDAGRSNKD-DMETEHLATLERLRQNQRPDYLSGSVSGSLQATDRLMKELRDIYRS 716
           +EM E+   S  D  +  E L  L++  +  R  +L G V GS+ A+DRLMKE+RDI+RS
Sbjct: 170 VEMAEEDPTSQHDVGVSKEGLDMLDKASKINRQQHLDGKVQGSITASDRLMKEIRDIHRS 229

Query: 717 HSFKNNMYS 743
             FKN +Y+
Sbjct: 230 EHFKNGIYT 238


>UniRef50_UPI0000F2BDCC Cluster: PREDICTED: similar to
           ubiquitin-conjugating enzyme E2Q; n=1; Monodelphis
           domestica|Rep: PREDICTED: similar to
           ubiquitin-conjugating enzyme E2Q - Monodelphis domestica
          Length = 547

 Score = 84.6 bits (200), Expect = 2e-15
 Identities = 39/63 (61%), Positives = 53/63 (84%), Gaps = 1/63 (1%)
 Frame = +3

Query: 561 GRSNKDD-METEHLATLERLRQNQRPDYLSGSVSGSLQATDRLMKELRDIYRSHSFKNNM 737
           G+ ++DD +  E+LA LE++++NQR DYL+G+VSGS+QATDRLMKELRDIYRS SFK   
Sbjct: 339 GKKSEDDGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQSFKGGN 398

Query: 738 YSI 746
           Y++
Sbjct: 399 YAV 401



 Score = 37.1 bits (82), Expect = 0.46
 Identities = 25/79 (31%), Positives = 40/79 (50%), Gaps = 21/79 (26%)
 Frame = +2

Query: 77  LKLEIKTLEQVFPKNHERFQIMSASVDELTCRFV-------------GKN--------GK 193
           L L++++ + +   +  RF+I SA +DEL+C F+             G +        G+
Sbjct: 166 LPLDLRSGDVMRLDDSWRFRIASACLDELSCEFLLAGAGAGAAGAAPGPHLPPRGPAPGE 225

Query: 194 KYEIHANITETYPTTPPVW 250
              IH NITE+YP  PP+W
Sbjct: 226 PVRIHCNITESYPAVPPIW 244


>UniRef50_Q7Z7E8 Cluster: Ubiquitin-conjugating enzyme E2 Q1; n=66;
           Eumetazoa|Rep: Ubiquitin-conjugating enzyme E2 Q1 - Homo
           sapiens (Human)
          Length = 422

 Score = 84.6 bits (200), Expect = 2e-15
 Identities = 39/63 (61%), Positives = 53/63 (84%), Gaps = 1/63 (1%)
 Frame = +3

Query: 561 GRSNKDD-METEHLATLERLRQNQRPDYLSGSVSGSLQATDRLMKELRDIYRSHSFKNNM 737
           G+ ++DD +  E+LA LE++++NQR DYL+G+VSGS+QATDRLMKELRDIYRS SFK   
Sbjct: 214 GKKSEDDGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQSFKGGN 273

Query: 738 YSI 746
           Y++
Sbjct: 274 YAV 276



 Score = 54.4 bits (125), Expect = 3e-06
 Identities = 31/79 (39%), Positives = 42/79 (53%), Gaps = 21/79 (26%)
 Frame = +2

Query: 77  LKLEIKTLEQVFPKNHERFQIMSASVDELTCRFV-------------GKN--------GK 193
           L+ E+K LE +F + HERF+I SA +DEL+C F+             G +        G 
Sbjct: 41  LRRELKLLESIFHRGHERFRIASACLDELSCEFLLAGAGGAGAGAAPGPHLPPRGSVPGD 100

Query: 194 KYEIHANITETYPTTPPVW 250
              IH NITE+YP  PP+W
Sbjct: 101 PVRIHCNITESYPAVPPIW 119


>UniRef50_Q4S862 Cluster: Chromosome 5 SCAF14709, whole genome
           shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
           Chromosome 5 SCAF14709, whole genome shotgun sequence -
           Tetraodon nigroviridis (Green puffer)
          Length = 297

 Score = 79.8 bits (188), Expect = 7e-14
 Identities = 36/58 (62%), Positives = 49/58 (84%)
 Frame = +3

Query: 555 DAGRSNKDDMETEHLATLERLRQNQRPDYLSGSVSGSLQATDRLMKELRDIYRSHSFK 728
           D  +S  D +E E+LA LE++R+NQR D+L+G+VSGS+QA+DRLMKELR+IYRS S+K
Sbjct: 23  DGKKSEDDGIEKENLAILEKIRKNQRQDHLNGAVSGSVQASDRLMKELREIYRSQSYK 80


>UniRef50_Q4S863 Cluster: Chromosome 5 SCAF14709, whole genome
           shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
           Chromosome 5 SCAF14709, whole genome shotgun sequence -
           Tetraodon nigroviridis (Green puffer)
          Length = 145

 Score = 74.5 bits (175), Expect = 2e-12
 Identities = 32/59 (54%), Positives = 41/59 (69%)
 Frame = +2

Query: 77  LKLEIKTLEQVFPKNHERFQIMSASVDELTCRFVGKNGKKYEIHANITETYPTTPPVWF 253
           LK E+K LE +F  NHERF+I+    DEL+C+F     K   IH NITE+YP+TPP+WF
Sbjct: 19  LKAELKFLESIFDPNHERFRIIDWKPDELSCQFNVTGDKLLIIHCNITESYPSTPPIWF 77


>UniRef50_UPI0000E1FDC5 Cluster: PREDICTED: similar to
           ubiquitin-conjugating enzyme UBCi; n=1; Pan
           troglodytes|Rep: PREDICTED: similar to
           ubiquitin-conjugating enzyme UBCi - Pan troglodytes
          Length = 115

 Score = 46.0 bits (104), Expect = 0.001
 Identities = 25/65 (38%), Positives = 40/65 (61%), Gaps = 6/65 (9%)
 Frame = +2

Query: 77  LKLEIKTLEQVFPKNHERFQIMSASVDELTCRF-VGKNGKKY-----EIHANITETYPTT 238
           L L+ ++ E +F KN ++F+I+S  +DEL C+F V   G  +      +H NITE+YP +
Sbjct: 16  LLLKYQSPEHIFSKNRKQFRIISWKLDELHCQFPVLLPGSLHLLPLLTLHCNITESYPPS 75

Query: 239 PPVWF 253
             +WF
Sbjct: 76  SLMWF 80


>UniRef50_Q54KZ9 Cluster: Putative uncharacterized protein; n=1;
           Dictyostelium discoideum AX4|Rep: Putative
           uncharacterized protein - Dictyostelium discoideum AX4
          Length = 417

 Score = 33.9 bits (74), Expect = 4.3
 Identities = 14/45 (31%), Positives = 26/45 (57%)
 Frame = -2

Query: 246 TGGVVGYVSVMFACISYFFPFLPTNLQVSSSTLADII*KRSWFFG 112
           +GGVVGY+     C+   + F PT ++++ +T+ ++    S  FG
Sbjct: 242 SGGVVGYIVGFALCLFIMYSFTPTMMEIAGATVMNLSLLTSDMFG 286


>UniRef50_Q2UPM5 Cluster: Predicted membrane proteins; n=16;
           Pezizomycotina|Rep: Predicted membrane proteins -
           Aspergillus oryzae
          Length = 502

 Score = 33.1 bits (72), Expect = 7.5
 Identities = 19/67 (28%), Positives = 31/67 (46%)
 Frame = -2

Query: 279 CHNWILTFSNQTGGVVGYVSVMFACISYFFPFLPTNLQVSSSTLADII*KRSWFFGNTCS 100
           C   + TFSN+   +  ++  +F  +S  F F P N     ST  D++    +FF   C 
Sbjct: 252 CFTSMFTFSNELVNIWSHLIGLFIVLSVAFYFYPLNPNFHLSTKTDVLIAAVFFFA-ACK 310

Query: 99  NVLISNL 79
            ++ S L
Sbjct: 311 CLVCSTL 317


>UniRef50_Q5L4T9 Cluster: Putative uncharacterized protein; n=7;
           Chlamydiaceae|Rep: Putative uncharacterized protein -
           Chlamydophila abortus
          Length = 437

 Score = 32.7 bits (71), Expect = 9.9
 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
 Frame = +3

Query: 618 RQNQRPDYLSGSVSGSLQATDRLMKELRD-IYRSHSF 725
           R+ Q  DYLSG   G L   D+L K+ R+ + R+H F
Sbjct: 354 RKEQLEDYLSGKTQGPLLVFDKLEKDSREFVLRAHVF 390


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 721,878,479
Number of Sequences: 1657284
Number of extensions: 13703293
Number of successful extensions: 35382
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 34238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35368
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 61323318355
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)