Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0809
(726 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 88 1e-18
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 28 1.2
SPBC1347.04 |tim54||TIM22 inner membrane protein import complex ... 28 1.6
SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|... 27 2.1
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 27 2.7
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 6.3
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch... 25 8.3
SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyce... 25 8.3
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 25 8.3
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 88.2 bits (209), Expect = 1e-18
Identities = 36/70 (51%), Positives = 56/70 (80%)
Frame = +3
Query: 510 SRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNPVDILTYVTWKISGLPKHRVIGSGTN 689
+R+DL++ N + K+I+ ++ KY+ D IL++A+NPVD+LTY T K++G P RVIGSGT
Sbjct: 104 TRMDLLKANISIFKEILREVTKYTKDAILLVATNPVDVLTYATLKLTGFPAERVIGSGTI 163
Query: 690 LDSARFRYLL 719
+D+ARF+YL+
Sbjct: 164 IDTARFQYLI 173
Score = 57.2 bits (132), Expect = 2e-09
Identities = 27/83 (32%), Positives = 42/83 (50%)
Frame = +1
Query: 262 KVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSST 441
K+ IVG G VG AF++L + I ++D+ K +GE MDL H + ++
Sbjct: 22 KIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNHAAPLSHETRVYLG- 80
Query: 442 DYSITAGSKICVVTAGVRQREGD 510
DY + V+TAG Q+ G+
Sbjct: 81 DYKDCKDATAVVITAGKNQKPGE 103
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 28.3 bits (60), Expect = 1.2
Identities = 21/66 (31%), Positives = 35/66 (53%), Gaps = 1/66 (1%)
Frame = +1
Query: 259 SKVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSA-FMRNAKIQS 435
S V +VG G VG+AA + + IA +D+ ADK E+ + G+ F+ ++K++
Sbjct: 195 STVAVVGCGCVGLAAMQGAVAAGASRIIA-IDINADK---EVYAKKFGATDFIDSSKVKD 250
Query: 436 STDYSI 453
Y I
Sbjct: 251 LVQYVI 256
>SPBC1347.04 |tim54||TIM22 inner membrane protein import complex
subunit Tim54|Schizosaccharomyces pombe|chr 2|||Manual
Length = 347
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/48 (25%), Positives = 21/48 (43%)
Frame = -2
Query: 653 PANLPRNIRQNIHGVTGDHQYCVRTVLYQLRDYLFKNIGVSLHEIETR 510
P LPR ++ +HG GD Y R + +F + +E++
Sbjct: 56 PLELPRKLKVYLHGPPGDGIYVAREEFEEYIRPIFNAAAIEFETVESK 103
>SPAC3A12.05c |taf2||TATA-binding protein associated factor
Taf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1174
Score = 27.5 bits (58), Expect = 2.1
Identities = 19/61 (31%), Positives = 32/61 (52%), Gaps = 6/61 (9%)
Frame = -3
Query: 661 LGSPLIFHVTYVRIST--GLLAITNIVSGLYF-ISCGII---CLRTSVFLCTRSRRDHLR 500
L SPL+ H+ R++ G L ++ ++ L + G + CL TS FL T + H+R
Sbjct: 470 LKSPLVIHILERRLTKTGGSLGMSRVIPKLLLQVMSGDMLNGCLSTSHFLKTCEKASHMR 529
Query: 499 V 497
+
Sbjct: 530 L 530
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 27.1 bits (57), Expect = 2.7
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +1
Query: 364 DKLKGEMMDLQHGSAFMRNAKIQSSTDYSITAGSKICV 477
+++K E+ +L H +R K Q+ DY+ SK+ +
Sbjct: 268 ERIKAEITELNHSLELLRVEKQQNDEDYTNIMKSKVAL 305
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 6.3
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -1
Query: 411 EC*SVLQVHHLSFQFVSHHVY 349
+C SV+ VH LSF V H++
Sbjct: 87 KCYSVIAVHRLSFTLVMFHLF 107
>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 867
Score = 25.4 bits (53), Expect = 8.3
Identities = 9/45 (20%), Positives = 23/45 (51%)
Frame = +3
Query: 540 DVLKQIIPQLIKYSPDTILVIASNPVDILTYVTWKISGLPKHRVI 674
D + ++P + + V +++P + +Y T SG+ +H ++
Sbjct: 333 DRVSNVLPSITSFDSSVTTVPSNSPATLNSYTTSVPSGMSRHPML 377
>SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 342
Score = 25.4 bits (53), Expect = 8.3
Identities = 20/72 (27%), Positives = 35/72 (48%), Gaps = 4/72 (5%)
Frame = -1
Query: 495 SNTSSNHADLRASCYRIIRTRLDLGV----PHEC*SVLQVHHLSFQFVSHHVY*SDVIRN 328
+NT++NH D C + +R RL+L + ++ S LQ + + H+ S+ + +
Sbjct: 120 TNTNNNHLDDIQCCAKNLRLRLELAMYKVQVNQTFSPLQDLPIVAKTKLHNCPNSEPVTS 179
Query: 327 ILRQHRESCGHP 292
I Q S G P
Sbjct: 180 IWNQRSLSSGKP 191
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 25.4 bits (53), Expect = 8.3
Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = +3
Query: 588 TILVIASNPVDILTYVTWKISGLPKHR-VIGSGTNLDSAR 704
TI I S P+D VTW + K+R V + N S+R
Sbjct: 644 TITNITSEPLDTPEQVTWLLDQASKNRSVAATNANEHSSR 683
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,857,466
Number of Sequences: 5004
Number of extensions: 57211
Number of successful extensions: 172
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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