Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0748
(779 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 152 2e-37
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 51 1e-06
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 51 1e-06
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 36 0.030
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 36 0.030
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 30 2.0
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 29 3.5
At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase fa... 29 4.6
At5g45720.1 68418.m05621 hypothetical protein 28 6.0
At5g40740.1 68418.m04944 expressed protein 28 8.0
At5g13340.1 68418.m01535 expressed protein 28 8.0
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 28 8.0
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 28 8.0
At2g43870.1 68415.m05454 polygalacturonase, putative / pectinase... 28 8.0
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 152 bits (369), Expect = 2e-37
Identities = 64/83 (77%), Positives = 73/83 (87%)
Frame = +2
Query: 509 VIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPV 688
VIVS ILERD H ++LWNTAV+I + GN+IGKHRKNHIPRVGDFNES YYMEG+TGHPV
Sbjct: 177 VIVSPILERDIDHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPV 236
Query: 689 FATRYGKIAVNICFGRHHVLNWM 757
F T +GKIAVNIC+GRHH LNW+
Sbjct: 237 FETVFGKIAVNICYGRHHPLNWL 259
Score = 111 bits (266), Expect = 7e-25
Identities = 51/85 (60%), Positives = 65/85 (76%)
Frame = +3
Query: 255 IVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPW 434
++Q+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W MPFAFCTRE++ W
Sbjct: 94 LIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTMPFAFCTRERR-W 152
Query: 435 CEFAESAEDGPTTTFLRELAIKYAM 509
CEFAE DG +T FL+ELA KY M
Sbjct: 153 CEFAEPV-DGESTKFLQELAKKYNM 176
Score = 34.3 bits (75), Expect = 0.092
Identities = 24/72 (33%), Positives = 34/72 (47%), Gaps = 3/72 (4%)
Frame = +1
Query: 49 SLESIINNNLTGRDLEEFNRIHFGR---RNNLEIKLKESSIXXXXXXXXXXXXXXFPAKD 219
SL +++ NL +E NR+ GR R+ +I L ES+ F A
Sbjct: 22 SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81
Query: 220 EQTRPPRIVKVG 255
EQ R PR+V+VG
Sbjct: 82 EQMRNPRVVRVG 93
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 50.8 bits (116), Expect = 1e-06
Identities = 22/57 (38%), Positives = 34/57 (59%)
Frame = +2
Query: 560 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICF 730
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+
Sbjct: 131 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICW 187
Score = 31.1 bits (67), Expect = 0.86
Identities = 20/81 (24%), Positives = 40/81 (49%)
Frame = +3
Query: 252 RIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP 431
R V S ++P + + F ++ A +G NII QEL+ + FC +++
Sbjct: 32 RFVSLSSSLPLSNYQSLPSSSSFKFPYALVREAHAKGANIILIQELFE-GYYFCQAQRED 90
Query: 432 WCEFAESAEDGPTTTFLRELA 494
+ + A+ ++ PT +++LA
Sbjct: 91 FFKRAKPYKNHPTIARMQKLA 111
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 50.8 bits (116), Expect = 1e-06
Identities = 22/57 (38%), Positives = 34/57 (59%)
Frame = +2
Query: 560 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICF 730
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+
Sbjct: 104 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICW 160
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 35.9 bits (79), Expect = 0.030
Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Frame = +2
Query: 539 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 703
E+ D L+NT V G + KHRK H IP F ES G T + T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDV 234
Query: 704 GKIAVNICF 730
G+I + IC+
Sbjct: 235 GRIGIGICY 243
Score = 33.5 bits (73), Expect = 0.16
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +3
Query: 294 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 467
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 468 TTTFLRELA 494
+T L E++
Sbjct: 154 STAMLSEVS 162
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 35.9 bits (79), Expect = 0.030
Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Frame = +2
Query: 539 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 703
E+ D L+NT V G + KHRK H IP F ES G T + T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDV 234
Query: 704 GKIAVNICF 730
G+I + IC+
Sbjct: 235 GRIGIGICY 243
Score = 33.5 bits (73), Expect = 0.16
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +3
Query: 294 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 467
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 468 TTTFLRELA 494
+T L E++
Sbjct: 154 STAMLSEVS 162
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.9 bits (64), Expect = 2.0
Identities = 21/64 (32%), Positives = 29/64 (45%)
Frame = +2
Query: 539 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 718
EK L+ TA+ S G +GKHRK +P + + PV+ T GKI
Sbjct: 127 EKDGYTLYCTALFFSPQGQFLGKHRK-VMPT--SLERCIWGQGDGSTIPVYDTPIGKIGA 183
Query: 719 NICF 730
IC+
Sbjct: 184 AICW 187
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 29.1 bits (62), Expect = 3.5
Identities = 20/64 (31%), Positives = 29/64 (45%)
Frame = +2
Query: 539 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 718
EK L+ TA+ S G +GKHRK +P + + PV+ T GK+
Sbjct: 120 EKDGYTLYCTALFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 176
Query: 719 NICF 730
IC+
Sbjct: 177 AICW 180
>At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase
family protein low similarity to 55 kDa type II
phosphatidylinositol 4-kinase [Rattus norvegicus]
GI:13660755; contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 533
Score = 28.7 bits (61), Expect = 4.6
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -2
Query: 553 VRMLLVPF*YRGHDHIAYLMASSRRKV 473
VR+ VPF RG DH AY +AS +R V
Sbjct: 187 VRISHVPFHDRGSDHAAYKVASLQRFV 213
>At5g45720.1 68418.m05621 hypothetical protein
Length = 900
Score = 28.3 bits (60), Expect = 6.0
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +2
Query: 575 VISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATR 700
V+ D+ IG+H +NHI + N+ N PV R
Sbjct: 9 VLKDSNGDIGEHLRNHIHLTNCIHLKNHMHNNNKQSPVLTDR 50
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 27.9 bits (59), Expect = 8.0
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +2
Query: 530 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESN 655
ER+ + ++ +++ NVIG N PRVG F SN
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSN 49
>At5g13340.1 68418.m01535 expressed protein
Length = 242
Score = 27.9 bits (59), Expect = 8.0
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = +2
Query: 161 SLQLRRLTSTSPHTLSRPRTSRPDPREL*R*DSSAFHRGAHRSSSQRAKE 310
S + RR S SP+T ++ P PR+ R SS+ HR + + KE
Sbjct: 27 SRRTRRDRSRSPYTSRHKKSRSPAPRQHQRDRSSSLSPSEHRIAIEVKKE 76
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 27.9 bits (59), Expect = 8.0
Identities = 19/64 (29%), Positives = 28/64 (43%)
Frame = +2
Query: 539 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 718
EK L+ T + S G +GKHRK +P + + PV+ T GK+
Sbjct: 5 EKEGYTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 61
Query: 719 NICF 730
IC+
Sbjct: 62 AICW 65
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 27.9 bits (59), Expect = 8.0
Identities = 19/64 (29%), Positives = 28/64 (43%)
Frame = +2
Query: 539 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 718
EK L+ T + S G +GKHRK +P + + PV+ T GK+
Sbjct: 127 EKEGYTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 183
Query: 719 NICF 730
IC+
Sbjct: 184 AICW 187
>At2g43870.1 68415.m05454 polygalacturonase, putative / pectinase,
putative similar to SP|P48979 Polygalacturonase
precursor (EC 3.2.1.15) (PG) (Pectinase) {Prunus
persica}; contains PF00295: Glycosyl hydrolases family
28 (polygalacturonases)
Length = 384
Score = 27.9 bits (59), Expect = 8.0
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
Frame = -2
Query: 754 PVQDVVPSEADVHRDLAVSGRKYRMAGVTFHVVVGFVKIADSR---NVVLAMFPDHVSGV 584
PV VVP + R + G K + VTF + V AD R N +F H+ G+
Sbjct: 53 PVTIVVPKGRFLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGI 112
Query: 583 TNY 575
T Y
Sbjct: 113 TVY 115
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,538,705
Number of Sequences: 28952
Number of extensions: 379123
Number of successful extensions: 1161
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1158
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1746037600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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