Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0728
(777 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 142 7e-35
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 142 7e-35
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 83 3e-17
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 67 3e-12
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 44 3e-05
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 38 0.001
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu... 29 0.74
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 26 5.2
SPBC23E6.09 |ssn6||transcriptional corepressor Ssn6|Schizosaccha... 26 5.2
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 26 5.2
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 6.9
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 26 6.9
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 26 6.9
SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|c... 25 9.2
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 142 bits (343), Expect = 7e-35
Identities = 69/86 (80%), Positives = 75/86 (87%)
Frame = +1
Query: 250 LSKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 429
L K+GT+TT + AHNMKVMKFSVSPVV+VAVE KN DLPKLVEGLKRL+KSDP V C
Sbjct: 463 LVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTT 522
Query: 430 EESGEHIVAGAGELHLEICLKDLEED 507
ESGEHIVAGAGELHLEICLKDL+ED
Sbjct: 523 SESGEHIVAGAGELHLEICLKDLQED 548
Score = 136 bits (330), Expect = 3e-33
Identities = 62/86 (72%), Positives = 72/86 (83%)
Frame = +2
Query: 2 MYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 181
+YVSKMVPTSD+GRFYAFGRVFSG V +G K RI GPN+ PGKK+DL+ K IQRT+LMMG
Sbjct: 380 IYVSKMVPTSDRGRFYAFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMG 439
Query: 182 RYVEAIEDVPSGNICGLVGVDQFLAR 259
+E IED P+GNI GLVGVDQFL +
Sbjct: 440 SRIEPIEDCPAGNIIGLVGVDQFLVK 465
Score = 111 bits (267), Expect = 1e-25
Identities = 52/89 (58%), Positives = 65/89 (73%)
Frame = +3
Query: 510 ACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPR 689
A IP+K S PVVSYRE+V+E S LSKSPNKHNR+FM A+PM + L I+ G VNPR
Sbjct: 550 AGIPLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPR 609
Query: 690 DDFKTRARYLTEKYEYDVTEARKICALAP 776
DDFK RAR + +++ +DVT+ARKI P
Sbjct: 610 DDFKVRARIMADEFGWDVTDARKIWCFGP 638
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 142 bits (343), Expect = 7e-35
Identities = 69/86 (80%), Positives = 75/86 (87%)
Frame = +1
Query: 250 LSKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 429
L K+GT+TT + AHNMKVMKFSVSPVV+VAVE KN DLPKLVEGLKRL+KSDP V C
Sbjct: 463 LVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTT 522
Query: 430 EESGEHIVAGAGELHLEICLKDLEED 507
ESGEHIVAGAGELHLEICLKDL+ED
Sbjct: 523 SESGEHIVAGAGELHLEICLKDLQED 548
Score = 136 bits (330), Expect = 3e-33
Identities = 62/86 (72%), Positives = 72/86 (83%)
Frame = +2
Query: 2 MYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 181
+YVSKMVPTSD+GRFYAFGRVFSG V +G K RI GPN+ PGKK+DL+ K IQRT+LMMG
Sbjct: 380 IYVSKMVPTSDRGRFYAFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMG 439
Query: 182 RYVEAIEDVPSGNICGLVGVDQFLAR 259
+E IED P+GNI GLVGVDQFL +
Sbjct: 440 SRIEPIEDCPAGNIIGLVGVDQFLVK 465
Score = 111 bits (267), Expect = 1e-25
Identities = 52/89 (58%), Positives = 65/89 (73%)
Frame = +3
Query: 510 ACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPR 689
A IP+K S PVVSYRE+V+E S LSKSPNKHNR+FM A+PM + L I+ G VNPR
Sbjct: 550 AGIPLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPR 609
Query: 690 DDFKTRARYLTEKYEYDVTEARKICALAP 776
DDFK RAR + +++ +DVT+ARKI P
Sbjct: 610 DDFKVRARIMADEFGWDVTDARKIWCFGP 638
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 83.4 bits (197), Expect = 3e-17
Identities = 39/83 (46%), Positives = 55/83 (66%)
Frame = +1
Query: 256 KTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEE 435
+T T+ + N N+ + + P+VRVA+EP P ++ KLV GL L ++DP VQ EE
Sbjct: 540 RTATLCSSPNGPNLVGVTQQMEPIVRVALEPVRPFEMNKLVTGLDMLNQADPCVQIAVEE 599
Query: 436 SGEHIVAGAGELHLEICLKDLEE 504
+GEH++ AGE+HLE CLKDL E
Sbjct: 600 NGEHVIMCAGEIHLERCLKDLRE 622
Score = 51.6 bits (118), Expect = 1e-07
Identities = 31/85 (36%), Positives = 43/85 (50%), Gaps = 4/85 (4%)
Frame = +2
Query: 32 DKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEK-TIQRTILMMGRYVEAIEDV 208
DK F R++SG + GQ+ + GP + P E K T++ LMMG+ + +E V
Sbjct: 464 DKDILIGFARIYSGTISVGQEVYVYGPKYDPVNPEKHITKVTVESLYLMMGQELVYLETV 523
Query: 209 PSGN---ICGLVGVDQFLARLVPSP 274
P+GN I GL G A L SP
Sbjct: 524 PAGNVFAIGGLAGTVLRTATLCSSP 548
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +3
Query: 510 ACIPIKKSDPVVSYRETVAEESDQLCLSK 596
A I I+ S P+V YRET D L +K
Sbjct: 625 AKIEIQASQPLVPYRETTIATPDLLAKNK 653
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 66.9 bits (156), Expect = 3e-12
Identities = 31/87 (35%), Positives = 48/87 (55%)
Frame = +3
Query: 516 IPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDD 695
I I+ SDPV + ET + S C S +PNK NR+ M +P+ G+ DI+ G+VN
Sbjct: 664 IEIRVSDPVARFCETAVDTSSIKCFSDTPNKKNRITMVVEPLEKGISNDIENGKVNINWP 723
Query: 696 FKTRARYLTEKYEYDVTEARKICALAP 776
K + + + Y++D+ +R I A P
Sbjct: 724 QKRISEFFQKNYDWDLLASRSIWAFGP 750
Score = 64.9 bits (151), Expect = 1e-11
Identities = 34/86 (39%), Positives = 53/86 (61%), Gaps = 3/86 (3%)
Frame = +1
Query: 250 LSKTGTITTFK---NAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ 420
+SKT TI + + + + + V +VAVEP NP++LPKL++GL++ KS P+
Sbjct: 572 ISKTATIVSDNLKDDPYIFRPIAHMSESVFKVAVEPHNPSELPKLLDGLRKTNKSYPLSI 631
Query: 421 CINEESGEHIVAGAGELHLEICLKDL 498
EESGEH + G GE++++ L DL
Sbjct: 632 TKVEESGEHTIFGTGEMYMDCLLYDL 657
Score = 59.7 bits (138), Expect = 5e-10
Identities = 29/86 (33%), Positives = 49/86 (56%)
Frame = +2
Query: 2 MYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 181
M+V+K+ T D FYAF RV+SG+V GQK +++G N++ +ED+ I +
Sbjct: 489 MHVTKLYNTVDANNFYAFARVYSGQVKKGQKVKVLGENYSLEDEEDMVVAHIAEICVPCA 548
Query: 182 RYVEAIEDVPSGNICGLVGVDQFLAR 259
RY ++ +G + L GVD +++
Sbjct: 549 RYRLHVDGAVAGMLVLLGGVDNSISK 574
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 43.6 bits (98), Expect = 3e-05
Identities = 25/82 (30%), Positives = 45/82 (54%), Gaps = 1/82 (1%)
Frame = +1
Query: 265 TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESG 441
T T ++ M M F PV+ ++++PK+ D + L R + DP + ++ ES
Sbjct: 456 TFTDGSVSYTMTSM-FVPEPVISLSLKPKSK-DTTSFSKALNRFQREDPTFRVQLDNESK 513
Query: 442 EHIVAGAGELHLEICLKDLEED 507
E I++G GELHLE+ ++ + +
Sbjct: 514 ETIISGMGELHLEVYVERMRRE 535
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/60 (30%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
Frame = +1
Query: 322 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINE-ESGEHIVAGAGELHLEICLKDL 498
PV ++EP + D P L+E L + + DP + + E+G+ ++ G G +HL++ + L
Sbjct: 443 PVCIASIEPYSLKDEPALLEALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQVSYERL 502
>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 241
Score = 29.1 bits (62), Expect = 0.74
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 6/49 (12%)
Frame = +1
Query: 355 PADLPKLVEGLKRLAKSD--PMVQCINEESGEHIVA----GAGELHLEI 483
P D+ KL EGL++L + D +VQ +NE + GE H+++
Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDL 220
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 26.2 bits (55), Expect = 5.2
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +3
Query: 651 LPEDIDEGRVNPRDDFKT 704
LPE IDEG+++ DD KT
Sbjct: 194 LPEKIDEGKLSDVDDGKT 211
>SPBC23E6.09 |ssn6||transcriptional corepressor
Ssn6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1102
Score = 26.2 bits (55), Expect = 5.2
Identities = 10/22 (45%), Positives = 18/22 (81%)
Frame = -2
Query: 653 QTIRHGLSLHEQTVVLVGRL*E 588
+++RH +SL+E+T + +GRL E
Sbjct: 324 ESVRHLISLNEETWIQIGRLAE 345
>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 871
Score = 26.2 bits (55), Expect = 5.2
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +3
Query: 588 LSKSPNKHNRLFMKAQPMPDGL 653
++KS KHN+L K +P+P L
Sbjct: 243 INKSVKKHNKLVKKHKPLPSTL 264
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.8 bits (54), Expect = 6.9
Identities = 19/68 (27%), Positives = 32/68 (47%)
Frame = +2
Query: 2 MYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 181
+YV VP D RFY + ++ + T + + +F P +D EK + L G
Sbjct: 2356 IYVWTNVPDCDISRFYCYTKMLGEWMFTLTEKTKLLESFLP---KDSLEKFSELQNLSTG 2412
Query: 182 RYVEAIED 205
+++AI D
Sbjct: 2413 LHMQAIWD 2420
>SPCC622.12c |||NADP-specific glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 451
Score = 25.8 bits (54), Expect = 6.9
Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +2
Query: 107 GPNFTP-GKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVG 238
G +F P GK ++ + Q + + RY+ DVP+G+I G+ G
Sbjct: 115 GSDFDPKGKSDNEIRRFSQAFMRQLFRYIGPQTDVPAGDI-GVTG 158
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 25.8 bits (54), Expect = 6.9
Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
Frame = +3
Query: 696 FKTRARYLTEKYEYDV--TEARKICAL 770
F++R RYLT YE+ V E+ +IC +
Sbjct: 1068 FESRRRYLTNLYEHIVLKAESHQICII 1094
>SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 716
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/24 (54%), Positives = 13/24 (54%)
Frame = -1
Query: 771 PKHKSYGLR*HHIRTFL*DTERES 700
PKHK LR H I TFL R S
Sbjct: 241 PKHKLTKLRSHRIATFLKGISRRS 264
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,353,217
Number of Sequences: 5004
Number of extensions: 70959
Number of successful extensions: 233
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 216
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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