Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0727
(783 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.12c |||conserved eukaryotic protein|Schizosaccharomyces... 46 6e-06
SPBC365.20c ||SPBC557.06c|nicotinamidase |Schizosaccharomyces po... 27 2.3
SPBC3E7.07c |||DUF757 family protein|Schizosaccharomyces pombe|c... 27 2.3
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 27 3.0
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 3.0
SPBC887.17 |||uracil permease |Schizosaccharomyces pombe|chr 2||... 27 3.0
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 27 4.0
SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|c... 26 5.3
SPCC965.08c |alr1||alanine racemase Alr1|Schizosaccharomyces pom... 26 5.3
SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual 26 5.3
SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 26 7.0
SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr ... 26 7.0
SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subuni... 26 7.0
>SPAC26F1.12c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 356
Score = 46.0 bits (104), Expect = 6e-06
Identities = 30/92 (32%), Positives = 52/92 (56%), Gaps = 4/92 (4%)
Frame = +2
Query: 518 SNLHYLAPMFSNLACSHRIRKWLC--EENPHV-PLIKLIPFCNYDVSNIRKGGAIGTIRN 688
+N +LA +F+++ R RK+ +E HV P KL+ F + S +R+ G I+N
Sbjct: 158 ANFDFLANVFADMTRFERGRKYFTTLQEYDHVIPASKLVVFTEHK-SLLRRTGVAAIIKN 216
Query: 689 ISFDTDYHEFLVSPD-LDLLTYILYP*WGNED 781
ISFD + + L+ + +++L Y+L P G E+
Sbjct: 217 ISFDIPFQKVLMDEEGINVLPYLLLPLAGPEE 248
Score = 29.5 bits (63), Expect = 0.57
Identities = 15/39 (38%), Positives = 20/39 (51%)
Frame = +3
Query: 315 KPLRYKNIIDLLISYVLNPNKTDADAACMTLSNITRLED 431
K L + L+ S V NP AD +CM L N+ + ED
Sbjct: 80 KVLMDDEFLQLIFSIVTNPLHGLADLSCMLLCNLAKEED 118
>SPBC365.20c ||SPBC557.06c|nicotinamidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 220
Score = 27.5 bits (58), Expect = 2.3
Identities = 24/86 (27%), Positives = 41/86 (47%), Gaps = 2/86 (2%)
Frame = +1
Query: 82 IEFLKPESRIDLRHISMDYLVSLSGTEDGVNKMLEHERITQAVIE--LTDDRIKEIAKHA 255
IEF+ P+ L + ++ G ++G+ +L+ + IT I TD +KE A HA
Sbjct: 113 IEFVIPKGSDRLVESYSGFYDAI-GRDNGLKAILDKKGITDVFIAGVATDICVKETALHA 171
Query: 256 S*CSLTYLPTRVALQSFSNTSHCAIK 333
TY+ + S + + + AIK
Sbjct: 172 RHWYNTYIISEAVKGSSTESHNQAIK 197
>SPBC3E7.07c |||DUF757 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 147
Score = 27.5 bits (58), Expect = 2.3
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Frame = +3
Query: 414 ITRLEDEL-EVCLNTFIPHLNDIMNA-FVNTEFKRPVRTCITWRPCL 548
+T+ +DE+ E +NTF P D A FVN + + + WRP L
Sbjct: 46 LTKYDDEIFEDLINTF-PEFKDPKTASFVNEDEMKSAKGKAAWRPFL 91
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 27.1 bits (57), Expect = 3.0
Identities = 21/84 (25%), Positives = 42/84 (50%)
Frame = +3
Query: 261 MLVNISAYTRGATELLKYKPLRYKNIIDLLISYVLNPNKTDADAACMTLSNITRLEDELE 440
+LV + ++ +G T ++ + Y++ ID S +K D + L NIT L + +
Sbjct: 273 LLVRLVSFAKGITLAKPFQHITYQHAIDKYGS-----DKPDIRFE-LPLKNITSLLPKQD 326
Query: 441 VCLNTFIPHLNDIMNAFVNTEFKR 512
++T I ND+ ++ N E ++
Sbjct: 327 PLISTEILVYNDLSHSLSNAESRK 350
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 3.0
Identities = 21/85 (24%), Positives = 42/85 (49%), Gaps = 7/85 (8%)
Frame = +3
Query: 261 MLVNISAYTRGATE-LLKYKPLRYKNIIDLLISYVLNPNKTDADAA------CMTLSNIT 419
+LVN+ YT G+TE + K + ++ II L+I + + A+ + S ++
Sbjct: 1662 LLVNVVKYTEGSTEGTVSSKSVLFRKIIALIIGVFAELHNSMAEFVHQKTFFRLFSSILS 1721
Query: 420 RLEDELEVCLNTFIPHLNDIMNAFV 494
L+D +V + F+ + I+ F+
Sbjct: 1722 ELDDAKDVLESCFVDIYSVILECFL 1746
>SPBC887.17 |||uracil permease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 625
Score = 27.1 bits (57), Expect = 3.0
Identities = 14/49 (28%), Positives = 28/49 (57%), Gaps = 3/49 (6%)
Frame = +1
Query: 97 PESRIDLRHISMDYLVSLSGTEDGVNKMLEHERITQ---AVIELTDDRI 234
P+ R + + +++ S S +EDG N+ HE +T ++ ++ D+RI
Sbjct: 543 PDDRKAMDNATLEMATSRSYSEDGKNEKTTHEDVTMKETSLKKMDDERI 591
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = -2
Query: 149 RLTR*SMDMCLKSILDSGFRNSINSSKESSAIVCNRI 39
R TR +++L++GFRNS +++SS + R+
Sbjct: 93 RRTRNRSQTQRRTLLENGFRNSRKKAQDSSNSIAERV 129
>SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 392
Score = 26.2 bits (55), Expect = 5.3
Identities = 17/55 (30%), Positives = 23/55 (41%), Gaps = 3/55 (5%)
Frame = +1
Query: 64 DSFDELIEFLKPESRIDLRHISMDYLVSLSGTEDGV---NKMLEHERITQAVIEL 219
+ FD L E KP+SR+ D G GV N L H R A+ ++
Sbjct: 68 ERFDWLCEAYKPKSRVPAFLTVFDIAGLTKGASTGVGLGNAFLSHVRAVDAIYQV 122
>SPCC965.08c |alr1||alanine racemase Alr1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 375
Score = 26.2 bits (55), Expect = 5.3
Identities = 25/95 (26%), Positives = 43/95 (45%), Gaps = 7/95 (7%)
Frame = +3
Query: 255 LLMLVNISAYTRGATELLKYKPLRYKNIIDLL----ISYVLNPNKTDADAACMTLSNITR 422
+L +V +AY GA ++ ++ L++ + ID I L + L
Sbjct: 33 VLAIVKANAYGHGAVQVARFL-LKHCSSIDGFGVSSIEEALELRHGGIYNKIVLLEGFFT 91
Query: 423 LEDELEVCLN---TFIPHLNDIMNAFVNTEFKRPV 518
EDEL++ + + I H D +N+F+ F RPV
Sbjct: 92 EEDELKLIDDYNFSIIIHSEDQVNSFIKYPFNRPV 126
>SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual
Length = 545
Score = 26.2 bits (55), Expect = 5.3
Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +2
Query: 584 LCEENPHVPLIKLIPFCNYDVSNIRKGGAIGTI-RNISFDTDYHEFLV 724
+C EN PL KL+P C RK G + ++ FDTD+H F +
Sbjct: 181 VCTENLS-PLFKLLP-CK------RKAGIASLLDSHLFFDTDWHSFSI 220
>SPCC576.10c |rpt3||19S proteasome regulatory subunit
Rpt3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 389
Score = 25.8 bits (54), Expect = 7.0
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +2
Query: 683 RNISFDTDYHEFLVSPDLDLLTYILYP 763
R + F T + L+SP++DL T+I+ P
Sbjct: 311 RRLVFQTITAKMLLSPEVDLDTFIMRP 337
>SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 977
Score = 25.8 bits (54), Expect = 7.0
Identities = 10/32 (31%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +2
Query: 518 SNLHYLAPMF-SNLACSHRIRKWLCEENPHVP 610
SN+ +++ + +L +H I KWL + H+P
Sbjct: 371 SNVDHMSSLILGSLKLAHAIMKWLVSDVEHIP 402
>SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subunit
Rpa2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1227
Score = 25.8 bits (54), Expect = 7.0
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +3
Query: 57 GARFFRRIDRIPETRVQDRLETHIHGLSRQPIGDGRR 167
G +++R+ + + Q R IH L+RQP+ +R
Sbjct: 1067 GVVYYQRLRHMVSDKFQVRTTGPIHNLTRQPVKGRKR 1103
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,238,942
Number of Sequences: 5004
Number of extensions: 68029
Number of successful extensions: 229
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 229
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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