Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0726
(625 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 138 4e-33
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 131 3e-31
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 129 1e-30
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 128 4e-30
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 110 8e-25
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 104 6e-23
At2g45830.1 68415.m05699 expressed protein 31 0.62
At1g04780.1 68414.m00474 ankyrin repeat family protein contains ... 31 0.82
At4g10530.1 68417.m01725 subtilase family protein contains simil... 29 2.5
At5g44760.1 68418.m05486 C2 domain-containing protein contains I... 27 7.7
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 138 bits (333), Expect = 4e-33
Identities = 60/81 (74%), Positives = 71/81 (87%)
Frame = +1
Query: 265 DNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVT 444
DNV E++PIVYTPTVG ACQKFG ++RRP+GLFI++ DKG + DVLKNWPE +++ IVVT
Sbjct: 119 DNVEELLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVT 178
Query: 445 DGERILGLGDLGACGMGIPVG 507
DGERILGLGDLG GMGIPVG
Sbjct: 179 DGERILGLGDLGCQGMGIPVG 199
Score = 69.3 bits (162), Expect = 2e-12
Identities = 30/50 (60%), Positives = 38/50 (76%)
Frame = +3
Query: 474 LGRMRDGHPCGQLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGL 623
LG G P G+LALY+ALGG++P CLP+TIDVGTN + +L+D YIGL
Sbjct: 189 LGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGL 238
Score = 44.8 bits (101), Expect = 5e-05
Identities = 24/61 (39%), Positives = 33/61 (54%)
Frame = +2
Query: 71 GYGFYHRRASVLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLF 250
G F + + GLLPP V Q+ Q + +I +Y+ PL KY+ L L +RNE LF
Sbjct: 54 GLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLF 113
Query: 251 Y 253
Y
Sbjct: 114 Y 114
Score = 27.5 bits (58), Expect = 7.7
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +3
Query: 33 SGLDHLKHPGLNKGMAFTIEERQS 104
SG L+ P NKG+AFT +ER +
Sbjct: 41 SGYSLLRDPRYNKGLAFTEKERDT 64
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 131 bits (317), Expect = 3e-31
Identities = 55/81 (67%), Positives = 70/81 (86%)
Frame = +1
Query: 265 DNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVT 444
DNV E++P+VYTPTVG ACQK+G +YRRP+GL+I++ +KG + +VLKNWP+ ++ IVVT
Sbjct: 126 DNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVT 185
Query: 445 DGERILGLGDLGACGMGIPVG 507
DGERILGLGDLG GMGIPVG
Sbjct: 186 DGERILGLGDLGCQGMGIPVG 206
Score = 68.5 bits (160), Expect = 3e-12
Identities = 31/50 (62%), Positives = 38/50 (76%)
Frame = +3
Query: 474 LGRMRDGHPCGQLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGL 623
LG G P G+L+LYTALGGI+P CLPITIDVGTN + +L++ YIGL
Sbjct: 196 LGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGL 245
Score = 45.6 bits (103), Expect = 3e-05
Identities = 24/61 (39%), Positives = 33/61 (54%)
Frame = +2
Query: 71 GYGFYHRRASVLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLF 250
G F + I GLLPP V +Q+ Q ++ +Y PL +Y+ LM L +RNE LF
Sbjct: 61 GLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLF 120
Query: 251 Y 253
Y
Sbjct: 121 Y 121
Score = 29.9 bits (64), Expect = 1.4
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +3
Query: 21 TSGLSGLDHLKHPGLNKGMAFTIEERQSWGSTG 119
TS SG ++ P NKG+AFT +ER + TG
Sbjct: 44 TSVASGYTLMRDPRYNKGLAFTDKERDAHYITG 76
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 129 bits (312), Expect = 1e-30
Identities = 53/81 (65%), Positives = 71/81 (87%)
Frame = +1
Query: 265 DNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVT 444
DNV E++P+VYTPTVG ACQK+G ++R+P+GL+I++++KG + +VLKNWP+ ++ IVVT
Sbjct: 126 DNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVT 185
Query: 445 DGERILGLGDLGACGMGIPVG 507
DGERILGLGDLG GMGIPVG
Sbjct: 186 DGERILGLGDLGCQGMGIPVG 206
Score = 70.5 bits (165), Expect = 8e-13
Identities = 32/50 (64%), Positives = 38/50 (76%)
Frame = +3
Query: 474 LGRMRDGHPCGQLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGL 623
LG G P G+L+LYTALGGI+P CLPITIDVGTN + +L+D YIGL
Sbjct: 196 LGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGL 245
Score = 44.4 bits (100), Expect = 6e-05
Identities = 22/61 (36%), Positives = 33/61 (54%)
Frame = +2
Query: 71 GYGFYHRRASVLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLF 250
G F + + GLLPP + +Q+ Q ++ +Y PL +Y+ LM L +RNE LF
Sbjct: 61 GLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLF 120
Query: 251 Y 253
Y
Sbjct: 121 Y 121
Score = 29.9 bits (64), Expect = 1.4
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +3
Query: 21 TSGLSGLDHLKHPGLNKGMAFTIEERQSWGSTG 119
TS SG ++ P NKG+AFT +ER + TG
Sbjct: 44 TSVASGYTLMRDPRYNKGLAFTDKERDAHYLTG 76
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 128 bits (308), Expect = 4e-30
Identities = 52/81 (64%), Positives = 71/81 (87%)
Frame = +1
Query: 265 DNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVT 444
D+V E++P++YTPTVG ACQK+G ++ RP+GLFI++ +KG +++VL+NWPE +++ IVVT
Sbjct: 184 DHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVT 243
Query: 445 DGERILGLGDLGACGMGIPVG 507
DGERILGLGDLG GMGIPVG
Sbjct: 244 DGERILGLGDLGCQGMGIPVG 264
Score = 69.7 bits (163), Expect = 1e-12
Identities = 30/50 (60%), Positives = 38/50 (76%)
Frame = +3
Query: 474 LGRMRDGHPCGQLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGL 623
LG G P G+L+LYTALGG++P CLP+TIDVGTN + +L+D YIGL
Sbjct: 254 LGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGL 303
Score = 52.4 bits (120), Expect = 2e-07
Identities = 25/61 (40%), Positives = 36/61 (59%)
Frame = +2
Query: 71 GYGFYHRRASVLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLF 250
G F HR + GLLPP V +Q+ QV+ ++ +Y+ PL KY+ +M L + NE LF
Sbjct: 119 GLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLF 178
Query: 251 Y 253
Y
Sbjct: 179 Y 179
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 110 bits (264), Expect = 8e-25
Identities = 45/81 (55%), Positives = 59/81 (72%)
Frame = +1
Query: 265 DNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVT 444
DN+ + PI+YTPTVGL CQ + +YRRPRG++ + DKG + ++ NWP V IV+T
Sbjct: 126 DNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYNWPAPQVDMIVIT 185
Query: 445 DGERILGLGDLGACGMGIPVG 507
DG RILGLGDLG G+GIP+G
Sbjct: 186 DGSRILGLGDLGVQGIGIPIG 206
Score = 52.4 bits (120), Expect = 2e-07
Identities = 23/50 (46%), Positives = 32/50 (64%)
Frame = +3
Query: 474 LGRMRDGHPCGQLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGL 623
LG G P G+L +Y A GI P + LPI +DVGTN + +L + LY+G+
Sbjct: 196 LGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDLYLGV 245
Score = 32.3 bits (70), Expect = 0.27
Identities = 27/69 (39%), Positives = 31/69 (44%), Gaps = 10/69 (14%)
Frame = +2
Query: 77 GFYHRRASVLGIHGLLPPRVKTQEEQVELCKLSIDRYEN----------PLNKYIYLMGL 226
GF LGI GLLPPRV T +Q + S EN L K+ L L
Sbjct: 53 GFPLTERDRLGIRGLLPPRVMTCVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRL 112
Query: 227 LDRNEHLFY 253
DRNE L+Y
Sbjct: 113 HDRNETLYY 121
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 104 bits (249), Expect = 6e-23
Identities = 45/81 (55%), Positives = 58/81 (71%)
Frame = +1
Query: 265 DNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVT 444
+N+ E PIVYTPTVGL CQ + ++RRPRG++ + D+G + ++ NWP V IVVT
Sbjct: 133 NNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVT 192
Query: 445 DGERILGLGDLGACGMGIPVG 507
DG RILGLGDLG G+GI VG
Sbjct: 193 DGSRILGLGDLGVHGIGIAVG 213
Score = 53.2 bits (122), Expect = 1e-07
Identities = 22/44 (50%), Positives = 30/44 (68%)
Frame = +3
Query: 492 GHPCGQLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGL 623
G G+L LY A GI P + LP+ IDVGTN + + +DP+Y+GL
Sbjct: 209 GIAVGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGL 252
Score = 31.1 bits (67), Expect = 0.62
Identities = 23/71 (32%), Positives = 31/71 (43%), Gaps = 10/71 (14%)
Frame = +2
Query: 71 GYGFYHRRASVLGIHGLLPPRVKTQEEQVELCKLSIDRYE----------NPLNKYIYLM 220
G F + L + GLLPP V E+Q+ + R E N L K+ L
Sbjct: 58 GTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPSDPNALAKWRILN 117
Query: 221 GLLDRNEHLFY 253
L DRNE ++Y
Sbjct: 118 RLHDRNETMYY 128
Score = 28.7 bits (61), Expect = 3.3
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = +3
Query: 36 GLDHLKHPGLNKGMAFTIEER 98
GLD L P NKG AFT+ ER
Sbjct: 46 GLDILHDPWFNKGTAFTMTER 66
>At2g45830.1 68415.m05699 expressed protein
Length = 523
Score = 31.1 bits (67), Expect = 0.62
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = +1
Query: 505 GNSRSTPRSEAS-SRISVCPSLSTWVRTPSRCW 600
G+SR+ + +S SRIS CPS W+ R W
Sbjct: 101 GSSRNNDKPRSSHSRISTCPSYFRWIHEDLRPW 133
>At1g04780.1 68414.m00474 ankyrin repeat family protein contains
Pfam PF00023: Ankyrin repeat
Length = 664
Score = 30.7 bits (66), Expect = 0.82
Identities = 18/67 (26%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 424 VRAIVV-TDGERILGLGDLGACGMGIPVGNSRSTPRSEASSRISVCPSLSTWVRTPSRCW 600
+R +V T E + + D P + +++PR E S + S W++TPS+
Sbjct: 551 IRVLVTFTKFEELEAIEDEFVTPPSSPTSSVKNSPREETQSSSNPSSSWFQWIKTPSQRP 610
Query: 601 TTRCTSG 621
+T +SG
Sbjct: 611 STSSSSG 617
>At4g10530.1 68417.m01725 subtilase family protein contains
similarity to subtilase; SP1 GI:9957714 from [Oryza
sativa]
Length = 747
Score = 29.1 bits (62), Expect = 2.5
Identities = 22/78 (28%), Positives = 32/78 (41%), Gaps = 1/78 (1%)
Frame = +1
Query: 394 DVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGNSRSTPRSEASSRISVCP-SLS 570
D+L +T V TD + +G A GIPV + S A + +V P L+
Sbjct: 283 DILSLSLQTSVPLFPETDARELTSVGAFHAVAKGIPVVAAASNAGPTAQTLSNVAPWVLT 342
Query: 571 TWVRTPSRCWTTRCTSGS 624
T R + T T G+
Sbjct: 343 VAATTQDRSFPTAITLGN 360
>At5g44760.1 68418.m05486 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 478
Score = 27.5 bits (58), Expect = 7.7
Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 6/32 (18%)
Frame = -3
Query: 311 PTVGVYTIGIISATLSAT------KGRTDAHC 234
PT+GV +G+ISA+ S +G TDA+C
Sbjct: 324 PTIGVLVLGVISASGSIPMKSRDGRGTTDAYC 355
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,333,016
Number of Sequences: 28952
Number of extensions: 347337
Number of successful extensions: 920
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 883
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 919
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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