Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0724
(770 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 89 6e-19
SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharo... 61 1e-10
SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosacchar... 40 5e-04
SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 32 0.10
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 29 0.74
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 28 1.7
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 3.9
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 26 5.2
SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex... 26 5.2
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 26 5.2
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 26 6.9
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 6.9
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 6.9
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 9.1
SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual 25 9.1
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 25 9.1
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 9.1
>SPAC23H4.09 |cdb4||curved DNA-binding protein
Cdb4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 381
Score = 89.0 bits (211), Expect = 6e-19
Identities = 42/88 (47%), Positives = 57/88 (64%), Gaps = 1/88 (1%)
Frame = +1
Query: 508 EISAEYGCKPIEGMLSHQLKQFRIDGEKSIIQNPSEAQRKEHEKATLEKYEVYAMDVLIS 687
+I+ YGCKP+ GMLSHQ ++ IDG+K +I NPS++QR E + T E+ EVY +D+L+S
Sbjct: 174 KIATSYGCKPVAGMLSHQQEREVIDGKKQVILNPSDSQRSEMDTFTFEEGEVYGVDILVS 233
Query: 688 TG-EAVGREMDTRCTIYKKTDEVYQFKL 768
T + D IYKKTD Y KL
Sbjct: 234 TSPSGKVKRSDIATRIYKKTDTTYMLKL 261
Score = 72.1 bits (169), Expect = 8e-14
Identities = 38/84 (45%), Positives = 48/84 (57%), Gaps = 2/84 (2%)
Frame = +2
Query: 266 HFSPIASEPD--YILKKGDLAKIDLGAHIDGFIAVVAHTVVVGESEVSGRAADVXXXXXX 439
H SP+ S+P+ LK GD+ KI LGAHIDGF ++VA T VV E V+G AADV
Sbjct: 91 HLSPLKSDPEANLALKSGDVVKILLGAHIDGFASLVATTTVVSEEPVTGPAADVIAAASA 150
Query: 440 XXXXXXXXXKPGTENYAVTEAIQK 511
KPG N+ VT+ + K
Sbjct: 151 ALKAAQRTIKPGNTNWQVTDIVDK 174
Score = 69.3 bits (162), Expect = 6e-13
Identities = 35/95 (36%), Positives = 55/95 (57%)
Frame = +3
Query: 6 EMADEKEVEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEET 185
E E V+ +++ V KYK+AG++ V+++V+ C P A +IC GD+L+ E
Sbjct: 5 EATSETAVDYSLSNPETVNKYKIAGEVSQNVIKKVVELCQPGAKIYDICVRGDELLNEAI 64
Query: 186 NKVFKKEKDSKKGIAFSTCVSVNTAFVTFRPLRAN 290
KV+ + KD+ KGIAF T VS N PL+++
Sbjct: 65 KKVY-RTKDAYKGIAFPTAVSPNDMAAHLSPLKSD 98
>SPBC23E6.05 |arx1||ribosomal export complex Arx1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 417
Score = 61.3 bits (142), Expect = 1e-10
Identities = 29/88 (32%), Positives = 49/88 (55%)
Frame = +3
Query: 15 DEKEVEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKV 194
D + + + +KY+ AG +V++ QV ++CVP AS REI +GD L+ E + +
Sbjct: 4 DPSDSNSRVVDASQFSKYRDAGALVSKAFHQVASRCVPGASTREISSYGDNLLHEYKSSI 63
Query: 195 FKKEKDSKKGIAFSTCVSVNTAFVTFRP 278
+K ++ +KGIA T + VN + P
Sbjct: 64 YKSQR-FEKGIAEPTSICVNNCAYNYAP 90
Score = 42.7 bits (96), Expect = 6e-05
Identities = 24/67 (35%), Positives = 38/67 (56%), Gaps = 1/67 (1%)
Frame = +2
Query: 188 QGFQKGERLQERHCIFNMRFCEHCICHFSPIA-SEPDYILKKGDLAKIDLGAHIDGFIAV 364
Q F+KG C+ N + + S IA ++ Y L+ GD+ KI +G H DG+ A+
Sbjct: 67 QRFEKGIAEPTSICVNNCAY-NYAPGPESVIAGNDNSYHLQVGDVTKISMGLHFDGYTAL 125
Query: 365 VAHTVVV 385
++HT+VV
Sbjct: 126 ISHTIVV 132
>SPBC14C8.03 |fma2||methionine aminopeptidase Fma2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 39.5 bits (88), Expect = 5e-04
Identities = 17/42 (40%), Positives = 27/42 (64%)
Frame = +2
Query: 254 HCICHFSPIASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTV 379
HC H++P A + ILK+ D+ K+D+G H++G I A T+
Sbjct: 175 HCAAHYTPNAGDTT-ILKEKDVMKVDIGVHVNGRIVDSAFTM 215
>SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 117
Score = 31.9 bits (69), Expect = 0.10
Identities = 21/63 (33%), Positives = 29/63 (46%), Gaps = 3/63 (4%)
Frame = -2
Query: 514 LFLNGFSDCIVLSTR---LQETKSRFTSMMSCQQNICSTSRDFTFTHNYCMSYNSYKSIN 344
L L G S I LST L + K+ + M + C S +T + N C +Y + SIN
Sbjct: 10 LGLLGLSTAIPLSTEADALLDRKTIYFGMKAYDDVNCKGSTSYTISFNKCQAYPNINSIN 69
Query: 343 VST 335
T
Sbjct: 70 ALT 72
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 29.1 bits (62), Expect = 0.74
Identities = 20/88 (22%), Positives = 39/88 (44%), Gaps = 1/88 (1%)
Frame = -1
Query: 344 CEHQDLSWLNLLFSECNPVRSQWAKSDKCSVHRNAC*K-CNAFLGVFLLFENLVSLLQYK 168
C + SW ++ E + +R++ + KC V +A + C + + + SLL++
Sbjct: 384 CSLLETSWYRIVLDEAHTIRNRDTLAAKCCVKLDAKYRWCLSGTPIQNHIDEFYSLLKFL 443
Query: 167 FIAKFTYFSC*CVWNAFCDDLLKDSVHY 84
I + CVW+ F D+ + Y
Sbjct: 444 RIKPY------CVWSLFAKDISRPLKSY 465
>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 881
Score = 27.9 bits (59), Expect = 1.7
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = -1
Query: 761 NWYTSSVFLYIVHLVSI 711
+W+TSSVFL+ LVSI
Sbjct: 432 HWFTSSVFLFTASLVSI 448
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 26.6 bits (56), Expect = 3.9
Identities = 14/48 (29%), Positives = 21/48 (43%)
Frame = -1
Query: 725 HLVSISRPTASPVDIRTSIAYTSYFSSVAFSCSLRCASDGFCIILFSP 582
H S + TASP+ +RTS ++Y + + S C S P
Sbjct: 208 HGFSNALSTASPIPVRTSSVSSTYLTQDREASSKNCLSKALAFSSIEP 255
>SPAC343.11c |msc1||multi-copy suppressor of Chk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1588
Score = 26.2 bits (55), Expect = 5.2
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +1
Query: 121 AFQTHQQEKYVNLAINLYWRRLTRFSKRRKTP 216
AF T +Q Y N NLY+ ++ S R TP
Sbjct: 474 AFPTSRQNAYYNDPWNLYFIHFSKLSPLRFTP 505
>SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex
subunit Tim13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 95
Score = 26.2 bits (55), Expect = 5.2
Identities = 12/40 (30%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
Frame = +3
Query: 15 DEKEV-EKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPD 131
D+K + K I ++L V + AG++++++ E KC+P+
Sbjct: 16 DKKSIFMKQIRQELAVAQ---AGELISKINENCFDKCIPE 52
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 26.2 bits (55), Expect = 5.2
Identities = 10/38 (26%), Positives = 19/38 (50%)
Frame = -3
Query: 609 WILYNTLLSINTELLQLV*EHTLNWLTTIFSTYF*MAS 496
W++Y+ L+ + E + V WL + T+F A+
Sbjct: 1095 WVIYHELIQTSKEYMHTVSTVNPKWLVEVAPTFFKFAN 1132
>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 25.8 bits (54), Expect = 6.9
Identities = 24/90 (26%), Positives = 42/90 (46%), Gaps = 1/90 (1%)
Frame = -2
Query: 418 ICSTSRDFTFTHNYCMSYNSYKSINVSTKIYLG*ISFFQNVIRFARN-GRKVTNAVFTET 242
I S S + HN ++ S ++N +L +FF ++FAR+ T+A ++ T
Sbjct: 290 IISDSPSSSIIHNPHPTHPSASAMNYMNPCFLEFHAFFNYPMKFARSRSAYSTSANYSNT 349
Query: 241 HVENAMPFLESFSFLKTLLVSSNTSLSPNS 152
N + + S ++ +S N S S NS
Sbjct: 350 PPRNTV----TPSTARSSTLSQNPSSSSNS 375
>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 357
Score = 25.8 bits (54), Expect = 6.9
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = -2
Query: 379 YCMSYNSYKSINVSTKIYLG*ISF 308
YC +YKS+ + T +Y+G F
Sbjct: 144 YCRKVLTYKSLGIRTAVYVGAFCF 167
>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 681
Score = 25.8 bits (54), Expect = 6.9
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -1
Query: 170 KFIAKFTYFSC*CVWNAFCDDLLKDSVHYLSC*LV 66
K + KF+ C +++A C+D L S H C +V
Sbjct: 472 KCLNKFSQEDCQFIFDAICEDPLDVSTHRHGCCVV 506
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 25.4 bits (53), Expect = 9.1
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +3
Query: 132 ASAREICEFGDKLVLEETNKVFKKEKD 212
++ +E FG+KL E T K+ EKD
Sbjct: 865 SNLQEKINFGNKLAKEHTEKISSLEKD 891
>SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 973
Score = 25.4 bits (53), Expect = 9.1
Identities = 18/57 (31%), Positives = 26/57 (45%)
Frame = -1
Query: 719 VSISRPTASPVDIRTSIAYTSYFSSVAFSCSLRCASDGFCIILFSPSIRNCFSWCES 549
V I PTA V + TS+ +SY ++VA + S G + + C S C S
Sbjct: 785 VMIVEPTAGYVTVTTSLGSSSYATTVATASG---TSTGTVLQVVPSLFPGCNSKCTS 838
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 25.4 bits (53), Expect = 9.1
Identities = 24/97 (24%), Positives = 34/97 (35%), Gaps = 2/97 (2%)
Frame = -2
Query: 364 NSYKSINVSTKIY--LG*ISFFQNVIRFARNGRKVTNAVFTETHVENAMPFLESFSFLKT 191
N + S ST++Y + I ++ R V A F V A+ +
Sbjct: 729 NLFASFQTSTRVYFVMEYILGGDLMVHIQRQQFSVKRARFYGAEVCLALKYFHENGIAYR 788
Query: 190 LLVSSNTSLSPNSHISLADASGTHFAMTCSRTRFTIC 80
L N L P+ HI +AD M T T C
Sbjct: 789 DLKLDNILLCPDGHIRIADYGLCKENMLLGNTTSTFC 825
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 25.4 bits (53), Expect = 9.1
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +2
Query: 287 EPDYILKKGDLAKIDLGAHIDGFIAVVAHTVV 382
EP+Y+L G+L+ +L ID F T V
Sbjct: 382 EPEYLLMYGNLSNEELVERIDAFYMNARRTYV 413
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,194,506
Number of Sequences: 5004
Number of extensions: 66505
Number of successful extensions: 228
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 225
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -