Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0701
(691 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 60 1e-09
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 57 1e-08
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 54 9e-08
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 53 2e-07
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 49 3e-06
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 47 1e-05
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 46 2e-05
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 46 2e-05
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 46 2e-05
At1g76940.1 68414.m08957 RNA recognition motif (RRM)-containing ... 30 1.7
At3g57480.1 68416.m06399 zinc finger (C2H2 type, AN1-like) famil... 28 5.1
At3g55950.1 68416.m06217 protein kinase family protein contains ... 28 5.1
At3g54960.1 68416.m06094 thioredoxin family protein similar to p... 28 5.1
At4g21370.1 68417.m03088 S-locus protein kinase, putative simila... 28 6.7
At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 28 6.7
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 60.5 bits (140), Expect = 1e-09
Identities = 33/91 (36%), Positives = 44/91 (48%)
Frame = +1
Query: 289 FLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVV 468
F + A+W T +F Y GLGIT HR AH+S+K L LL +A Q +
Sbjct: 61 FTWSALWVTFLF----YTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPI 116
Query: 469 DWARDHRMHHKYSGPMRTPTTQPEDSSFPTL 561
DW HR HH+++ R P + E F L
Sbjct: 117 DWVSTHRYHHQFTDSERDPHSPKEGFWFSHL 147
Score = 35.9 bits (79), Expect = 0.025
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = +3
Query: 498 QIFRTDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 662
Q ++ DPH+ GF+FSH+ W+ + K G +V DL+ RF +K
Sbjct: 127 QFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC-GRRANVEDLKRQWFYRFLQK 180
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 56.8 bits (131), Expect = 1e-08
Identities = 29/89 (32%), Positives = 39/89 (43%)
Frame = +1
Query: 295 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 474
F W A+ +Y GLGIT HR AH+S+K L +A Q +DW
Sbjct: 57 FNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDW 116
Query: 475 ARDHRMHHKYSGPMRTPTTQPEDSSFPTL 561
HR HH+++ R P + E F L
Sbjct: 117 VSTHRYHHQFTDSDRDPHSPNEGFWFSHL 145
Score = 35.9 bits (79), Expect = 0.025
Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 2/62 (3%)
Frame = +3
Query: 498 QIFRTDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK--YYQ 671
Q +D DPH+ GF+FSH+ WL + ++ G +V DL+ +F ++ Y
Sbjct: 125 QFTDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVLYH 183
Query: 672 IL 677
IL
Sbjct: 184 IL 185
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 54.0 bits (124), Expect = 9e-08
Identities = 30/93 (32%), Positives = 39/93 (41%)
Frame = +1
Query: 295 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 474
F W F LY + L IT HR AH+S+K L L F A Q +DW
Sbjct: 51 FNYKWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDW 110
Query: 475 ARDHRMHHKYSGPMRTPTTQPEDSSFPTLAGCC 573
HR HH+++ R P + E F + C
Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWIC 143
Score = 36.3 bits (80), Expect = 0.019
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +3
Query: 498 QIFRTDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLR 632
Q +D DPH+ GF+FSH+ W+ ++ + K G +V DL+
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRN-NVMDLK 162
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 52.8 bits (121), Expect = 2e-07
Identities = 28/78 (35%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
Frame = +1
Query: 295 FKAMWRTSIFAIFLYLCSGL-GITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVD 471
F+ WR A LY+ +GL GIT HR +HK++K L L A Q +D
Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180
Query: 472 WARDHRMHHKYSGPMRTP 525
W HR HH++ R P
Sbjct: 181 WVSTHRYHHQFCDSDRDP 198
Score = 43.2 bits (97), Expect = 2e-04
Identities = 24/64 (37%), Positives = 33/64 (51%)
Frame = +3
Query: 498 QIFRTDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 677
Q +D DPH+ GF+FSH+ W + + + G +V DL P RF + Y IL
Sbjct: 190 QFCDSDRDPHSPLDGFWFSHMNW-MFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTY-IL 247
Query: 678 MPLA 689
PLA
Sbjct: 248 HPLA 251
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 48.8 bits (111), Expect = 3e-06
Identities = 24/77 (31%), Positives = 32/77 (41%)
Frame = +1
Query: 295 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 474
F W F + L + + L IT HR HKS+K L A Q +DW
Sbjct: 51 FNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 475 ARDHRMHHKYSGPMRTP 525
HR HH+++ R P
Sbjct: 111 VSTHRFHHQFTDSDRDP 127
Score = 36.3 bits (80), Expect = 0.019
Identities = 18/52 (34%), Positives = 26/52 (50%)
Frame = +3
Query: 498 QIFRTDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 653
Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 46.8 bits (106), Expect = 1e-05
Identities = 23/78 (29%), Positives = 37/78 (47%)
Frame = +1
Query: 331 FLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDWARDHRMHHKYSG 510
+L +G+ IT HR +H+S+ L L +AFQ ++W +HR HHK+
Sbjct: 57 WLVFINGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCE 116
Query: 511 PMRTPTTQPEDSSFPTLA 564
R P + + F +A
Sbjct: 117 TQRDPHSPTQGFWFSHMA 134
Score = 39.1 bits (87), Expect = 0.003
Identities = 18/51 (35%), Positives = 29/51 (56%)
Frame = +3
Query: 510 TDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 662
T DPH+ T+GF+FSH+ W + ++ G +V+DL P RF ++
Sbjct: 117 TQRDPHSPTQGFWFSHMAW-IFDTSSILENCGGEENVDDLVRQPFYRFLQR 166
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 46.4 bits (105), Expect = 2e-05
Identities = 25/86 (29%), Positives = 36/86 (41%)
Frame = +1
Query: 295 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 474
F W F + L+ + L IT HR +H+S+K L A Q +DW
Sbjct: 52 FNFKWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDW 111
Query: 475 ARDHRMHHKYSGPMRTPTTQPEDSSF 552
HR HH+++ R P + E F
Sbjct: 112 VSIHRFHHQFTDSDRDPHSPKEGLLF 137
Score = 34.3 bits (75), Expect = 0.077
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +3
Query: 498 QIFRTDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 662
Q +D DPH+ G FSHI W+ ++ + K G +V DL+ +F ++
Sbjct: 120 QFTDSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRD-NVLDLKKQWFYKFLRR 173
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 46.0 bits (104), Expect = 2e-05
Identities = 23/77 (29%), Positives = 32/77 (41%)
Frame = +1
Query: 295 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 474
F W FA + + + L IT HR H+S+K L A Q +DW
Sbjct: 51 FNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 475 ARDHRMHHKYSGPMRTP 525
HR HH+++ R P
Sbjct: 111 VSTHRFHHQFTDSDRDP 127
Score = 36.3 bits (80), Expect = 0.019
Identities = 18/52 (34%), Positives = 26/52 (50%)
Frame = +3
Query: 498 QIFRTDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 653
Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 46.0 bits (104), Expect = 2e-05
Identities = 24/77 (31%), Positives = 33/77 (42%)
Frame = +1
Query: 295 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 474
F W F I L + + L IT HR H+S+K L +A Q +DW
Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110
Query: 475 ARDHRMHHKYSGPMRTP 525
HR HH+++ R P
Sbjct: 111 VSIHRFHHQFTDSDRDP 127
Score = 38.3 bits (85), Expect = 0.005
Identities = 20/55 (36%), Positives = 28/55 (50%)
Frame = +3
Query: 498 QIFRTDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 662
Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF KK
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREKC-GRRNNVMDLKQQWFYRFLKK 172
>At1g76940.1 68414.m08957 RNA recognition motif (RRM)-containing
protein contains Pfam PF00076: RNA recognition motif.
(a.k.a. RRM, RBD, or RNP domain); similar to RNA-binding
protein with multiple splicing homolog (RBP-MS) (HEart,
RRM Expressed Sequence) (Hermes) (Swiss-Prot:Q9YGI5)
[Xenopus laevis]; similar to RNA-binding protein with
multiple splicing (RBP-MS) (Swiss-Prot:Q93062) [Homo
sapiens]
Length = 233
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/49 (28%), Positives = 21/49 (42%)
Frame = +1
Query: 472 WARDHRMHHKYSGPMRTPTTQPEDSSFPTLAGCC*GSIPKSKPKAIPST 618
W + + HH GPM+ P + E S G G P+ + +P T
Sbjct: 6 WNQQRQQHHPPGGPMKRPRSDFEAPSSTMTIGHGGGYYPRDEDLDVPDT 54
>At3g57480.1 68416.m06399 zinc finger (C2H2 type, AN1-like) family
protein contains Pfam domain, PF00096: Zinc finger, C2H2
type; contains Pfam domain, PF01428: AN1-like Zinc
finger
Length = 249
Score = 28.3 bits (60), Expect = 5.1
Identities = 19/48 (39%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
Frame = +1
Query: 385 AHKSYKARLP-LRILLTIFNTIAFQDAVVDWARDHRM--HHKYSGPMR 519
A K K +P R LLT NTI +D +D HR H SGP +
Sbjct: 95 AVKKKKCPVPRCRELLTFSNTIKCRDCSIDHCLKHRFGPDHSCSGPKK 142
>At3g55950.1 68416.m06217 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
cytokinin-regulated kinase 1 [Nicotiana tabacum]
gi|10998537|gb|AAG25966
Length = 814
Score = 28.3 bits (60), Expect = 5.1
Identities = 15/41 (36%), Positives = 18/41 (43%)
Frame = +2
Query: 170 PEDAPPHVPPSAEKRPWKIVWRNVILFFICMSVVFMEGTCS 292
P PP PS P K + R ++ F I SV G CS
Sbjct: 372 PPPPPPPPSPSTSSPPSKALTRGLLAFAIVGSVGAFAGICS 412
>At3g54960.1 68416.m06094 thioredoxin family protein similar to
protein disulfide isomerase GI:5902592 from [Volvox
carteri f. nagariensis], GI:2708314 from Chlamydomonas
reinhardtii; contains Pfam profile: PF00085 Thioredoxin
Length = 579
Score = 28.3 bits (60), Expect = 5.1
Identities = 10/41 (24%), Positives = 21/41 (51%)
Frame = -2
Query: 327 GEYRSPPHGFEKEQVPSINTTDMQIKNRITFLHTIFQGLFS 205
GE+ HG+E+E +P ++ D+ + + F + F+
Sbjct: 79 GEFHHGDHGYEEEPLPPVDEKDVAVLTKDNFTEFVGNNSFA 119
>At4g21370.1 68417.m03088 S-locus protein kinase, putative similar
to SRKa [Arabidopsis lyrata] gi|13620927|dbj|BAB40986;
contains Pfam domains PF00954: S-locus glycoprotein
family, PF00069: Protein kinase domain and PF01453:
Lectin (probable mannose binding)
Length = 844
Score = 27.9 bits (59), Expect = 6.7
Identities = 14/48 (29%), Positives = 24/48 (50%)
Frame = +3
Query: 129 RLRGYCTKPTPITCQKMHRPMYLPQQRRDLGRLCGEM*FCSLSACRWC 272
RL G+ +PT Q+ + ++P+ DL +CG +C +S C
Sbjct: 273 RLEGFTWEPTQ---QEWNMFWFMPKDTCDLYGICGPYAYCDMSTSPTC 317
>At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:7381227;
contains PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 468
Score = 27.9 bits (59), Expect = 6.7
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = +2
Query: 134 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWR 235
S+ ++ D +P D P P S KR W +R
Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYR 157
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,133,195
Number of Sequences: 28952
Number of extensions: 410213
Number of successful extensions: 1159
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1097
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1157
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -