Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0683
         (692 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo...    75   7e-15
SPAC23D3.04c |gpd2||glycerol-3-phosphate dehydrogenase Gpd2|Schi...    71   2e-13
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|...    29   0.84 
SPCC2H8.03 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual         27   1.9  
SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizo...    26   4.5  
SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyce...    26   5.9  

>SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase
           Gpd1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 385

 Score = 75.4 bits (177), Expect = 7e-15
 Identities = 41/70 (58%), Positives = 49/70 (70%), Gaps = 7/70 (10%)
 Frame = +3

Query: 63  QPKNKVCI--VGSGNWGSAIAKIVGRNA-ASLSNFEDRVTMWVYEEIIEGK----KLTEI 221
           +PK ++ I  VGSGNWG+AIAKI G NA A   +F  +V MWV+EE IE K    KLTE+
Sbjct: 18  RPKKRLSIGVVGSGNWGTAIAKICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEV 77

Query: 222 INETHENVKY 251
            NE HENVKY
Sbjct: 78  FNEAHENVKY 87



 Score = 72.1 bits (169), Expect = 7e-14
 Identities = 30/61 (49%), Positives = 44/61 (72%)
 Frame = +2

Query: 257 GHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDIA 436
           G + P NV+AVPDV E A+ AD+L+FVVPHQF+  +C  ++G I+P A  +S IKG  ++
Sbjct: 90  GIECPPNVIAVPDVREVARRADILVFVVPHQFIERVCDQMVGLIRPGAVGISCIKGVAVS 149

Query: 437 E 439
           +
Sbjct: 150 K 150



 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 15/21 (71%), Positives = 20/21 (95%)
 Frame = +3

Query: 501 INGANIASEVAEEKFCETTIG 563
           ++GAN+A+EVA E+FCETTIG
Sbjct: 171 LSGANVANEVAREQFCETTIG 191


>SPAC23D3.04c |gpd2||glycerol-3-phosphate dehydrogenase
           Gpd2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 373

 Score = 70.9 bits (166), Expect = 2e-13
 Identities = 39/63 (61%), Positives = 45/63 (71%), Gaps = 5/63 (7%)
 Frame = +3

Query: 78  VCIVGSGNWGSAIAKIVGRNA-ASLSNFEDRVTMWVYEEII--EGKK--LTEIINETHEN 242
           V I+GSGNWG+AIAKI G NA A    F  +V MW+YEE I  EGK+  LTE+ N THEN
Sbjct: 27  VGIIGSGNWGTAIAKICGENAKAHPDIFHPQVHMWMYEEKIQHEGKECNLTEVFNTTHEN 86

Query: 243 VKY 251
           VKY
Sbjct: 87  VKY 89



 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 28/61 (45%), Positives = 40/61 (65%)
 Frame = +2

Query: 257 GHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDIA 436
           G K PSNV A PD+ +    +D+L++V+PHQFV  IC+ L G +K  A A+S IKG  + 
Sbjct: 92  GIKCPSNVFANPDIRDVGSRSDILVWVLPHQFVVRICNQLKGCLKKDAVAISCIKGVSVT 151

Query: 437 E 439
           +
Sbjct: 152 K 152



 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 18/21 (85%), Positives = 21/21 (100%)
 Frame = +3

Query: 501 INGANIASEVAEEKFCETTIG 563
           ++GANIASEVA+EKFCETTIG
Sbjct: 173 LSGANIASEVAQEKFCETTIG 193


>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 531

 Score = 28.7 bits (61), Expect = 0.84
 Identities = 10/36 (27%), Positives = 22/36 (61%)
 Frame = +2

Query: 347 QFVRTICSTLLGKIKPTAAALSLIKGFDIAEVVASI 454
           + +R IC T+ G ++ +   + L KGF++ E + ++
Sbjct: 315 ELIRFICLTINGNLQISGQTVDLEKGFEVIEFIPAL 350


>SPCC2H8.03 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 75

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = -2

Query: 253 RYLTFS*VSLIISVNFFPSIISSYTHIVTLSSK 155
           RY TFS + L+   N FP  ISS+  ++T   K
Sbjct: 26  RYATFSILKLV-EYNCFPGFISSFHSLLTYRKK 57


>SPBC336.14c |ppk26||serine/threonine protein kinase
           Ppk26|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 589

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = -2

Query: 217 SVNFFPSIISSYTHIVTLSSKFDR 146
           +++  PSIISSYT +  L++K  R
Sbjct: 206 TISALPSIISSYTSLAPLNTKLYR 229


>SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1297

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
 Frame = -2

Query: 445 HHFGYIKSLNQRQSSCSWLYFSKQSRADSSDKLMRHHKY*KISIFCSF--NYIWNSNNIR 272
           HH+ YI  L++  +  SW Y        + D L+    Y K+  F       +W+ N I 
Sbjct: 89  HHYNYITQLSEDITQLSWGY--------NFDLLLCASSYTKLINFSEDKPRLVWSQNRIS 140

Query: 271 RQFVAS 254
             F++S
Sbjct: 141 DSFLSS 146


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,575,186
Number of Sequences: 5004
Number of extensions: 48253
Number of successful extensions: 163
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)