Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0677
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 127 8e-30
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 123 1e-28
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 121 5e-28
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 117 8e-27
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 98 5e-21
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 93 2e-19
At2g45830.1 68415.m05699 expressed protein 29 2.8
At3g56920.1 68416.m06331 zinc finger (DHHC type) family protein ... 29 3.8
At1g80600.1 68414.m09457 acetylornithine aminotransferase, mitoc... 28 5.0
At5g44040.1 68418.m05389 expressed protein similar to unknown pr... 28 6.6
At1g04780.1 68414.m00474 ankyrin repeat family protein contains ... 28 6.6
At5g64430.1 68418.m08093 octicosapeptide/Phox/Bem1p (PB1) domain... 27 8.7
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 127 bits (306), Expect = 8e-30
Identities = 56/93 (60%), Positives = 73/93 (78%)
Frame = +2
Query: 230 RQIRKST*QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG 409
RQ + +Y+ L L +RNE LFY+ + DNV E++PIVYTPTVG ACQKFG ++RRP+G
Sbjct: 90 RQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQG 149
Query: 410 LFITIHDKGHVYDVLKNWPETDVRAIVVTDGER 508
LFI++ DKG + DVLKNWPE +++ IVVTDGER
Sbjct: 150 LFISLKDKGKILDVLKNWPERNIQVIVVTDGER 182
Score = 94.7 bits (225), Expect = 5e-20
Identities = 41/56 (73%), Positives = 48/56 (85%)
Frame = +1
Query: 511 LGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGLR 678
LGLGDLG GMGIPVGKLALY+ALGG++P CLP+TIDVGTN + +L+D YIGLR
Sbjct: 184 LGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLR 239
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +1
Query: 85 SGLDHLKHPGLNKGMAFTIEERQS 156
SG L+ P NKG+AFT +ER +
Sbjct: 41 SGYSLLRDPRYNKGLAFTEKERDT 64
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 123 bits (296), Expect = 1e-28
Identities = 50/85 (58%), Positives = 70/85 (82%)
Frame = +2
Query: 254 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 433
+Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G +YRRP+GL+I++ +K
Sbjct: 105 RYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEK 164
Query: 434 GHVYDVLKNWPETDVRAIVVTDGER 508
G + +VLKNWP+ ++ IVVTDGER
Sbjct: 165 GKILEVLKNWPQRGIQVIVVTDGER 189
Score = 92.7 bits (220), Expect = 2e-19
Identities = 41/56 (73%), Positives = 48/56 (85%)
Frame = +1
Query: 511 LGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGLR 678
LGLGDLG GMGIPVGKL+LYTALGGI+P CLPITIDVGTN + +L++ YIGL+
Sbjct: 191 LGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLK 246
Score = 29.9 bits (64), Expect = 1.6
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +1
Query: 73 TSGLSGLDHLKHPGLNKGMAFTIEERQSWGSTG 171
TS SG ++ P NKG+AFT +ER + TG
Sbjct: 44 TSVASGYTLMRDPRYNKGLAFTDKERDAHYITG 76
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 121 bits (291), Expect = 5e-28
Identities = 48/85 (56%), Positives = 71/85 (83%)
Frame = +2
Query: 254 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 433
+Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+GL+I++++K
Sbjct: 105 RYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEK 164
Query: 434 GHVYDVLKNWPETDVRAIVVTDGER 508
G + +VLKNWP+ ++ IVVTDGER
Sbjct: 165 GKILEVLKNWPQRGIQVIVVTDGER 189
Score = 94.7 bits (225), Expect = 5e-20
Identities = 42/56 (75%), Positives = 48/56 (85%)
Frame = +1
Query: 511 LGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGLR 678
LGLGDLG GMGIPVGKL+LYTALGGI+P CLPITIDVGTN + +L+D YIGL+
Sbjct: 191 LGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLK 246
Score = 29.9 bits (64), Expect = 1.6
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +1
Query: 73 TSGLSGLDHLKHPGLNKGMAFTIEERQSWGSTG 171
TS SG ++ P NKG+AFT +ER + TG
Sbjct: 44 TSVASGYTLMRDPRYNKGLAFTDKERDAHYLTG 76
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 117 bits (281), Expect = 8e-27
Identities = 47/93 (50%), Positives = 73/93 (78%)
Frame = +2
Query: 230 RQIRKST*QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG 409
RQ + +Y+ +M L + NE LFY+ + D+V E++P++YTPTVG ACQK+G ++ RP+G
Sbjct: 155 RQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQG 214
Query: 410 LFITIHDKGHVYDVLKNWPETDVRAIVVTDGER 508
LFI++ +KG +++VL+NWPE +++ IVVTDGER
Sbjct: 215 LFISLKEKGKIHEVLRNWPEKNIQVIVVTDGER 247
Score = 95.1 bits (226), Expect = 4e-20
Identities = 41/56 (73%), Positives = 48/56 (85%)
Frame = +1
Query: 511 LGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGLR 678
LGLGDLG GMGIPVGKL+LYTALGG++P CLP+TIDVGTN + +L+D YIGLR
Sbjct: 249 LGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLR 304
Score = 33.1 bits (72), Expect = 0.17
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = +3
Query: 123 GYGFYHRRASVLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 251
G F HR + GLLPP V +Q+ QV+ ++ +Y+ PL
Sbjct: 119 GLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPL 161
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 97.9 bits (233), Expect = 5e-21
Identities = 40/78 (51%), Positives = 54/78 (69%)
Frame = +2
Query: 275 LLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVL 454
L DRNE L+YR + DN+ + PI+YTPTVGL CQ + +YRRPRG++ + DKG + ++
Sbjct: 112 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMI 171
Query: 455 KNWPETDVRAIVVTDGER 508
NWP V IV+TDG R
Sbjct: 172 YNWPAPQVDMIVITDGSR 189
Score = 75.8 bits (178), Expect = 3e-14
Identities = 32/56 (57%), Positives = 42/56 (75%)
Frame = +1
Query: 511 LGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGLR 678
LGLGDLG G+GIP+GKL +Y A GI P + LPI +DVGTN + +L + LY+G+R
Sbjct: 191 LGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDLYLGVR 246
Score = 29.1 bits (62), Expect = 2.8
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = +3
Query: 129 GFYHRRASVLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNST 260
GF LGI GLLPPRV T +Q C I+ + + N+T
Sbjct: 53 GFPLTERDRLGIRGLLPPRVMTCVQQ---CDRFIESFRSLENNT 93
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 92.7 bits (220), Expect = 2e-19
Identities = 38/78 (48%), Positives = 54/78 (69%)
Frame = +2
Query: 275 LLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVL 454
L DRNE ++Y+ + +N+ E PIVYTPTVGL CQ + ++RRPRG++ + D+G + ++
Sbjct: 119 LHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMV 178
Query: 455 KNWPETDVRAIVVTDGER 508
NWP V IVVTDG R
Sbjct: 179 YNWPAEQVDMIVVTDGSR 196
Score = 76.2 bits (179), Expect = 2e-14
Identities = 33/56 (58%), Positives = 42/56 (75%)
Frame = +1
Query: 511 LGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGLR 678
LGLGDLG G+GI VGKL LY A GI P + LP+ IDVGTN + + +DP+Y+GL+
Sbjct: 198 LGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQ 253
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = +1
Query: 88 GLDHLKHPGLNKGMAFTIEER 150
GLD L P NKG AFT+ ER
Sbjct: 46 GLDILHDPWFNKGTAFTMTER 66
>At2g45830.1 68415.m05699 expressed protein
Length = 523
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 557 ANSRSTPRSEASSRISVCPSLSTWVRTPSRCW 652
+ + PRS + SRIS CPS W+ R W
Sbjct: 103 SRNNDKPRS-SHSRISTCPSYFRWIHEDLRPW 133
>At3g56920.1 68416.m06331 zinc finger (DHHC type) family protein
contains Pfam profile PF01529: DHHC zinc finger domain
Length = 338
Score = 28.7 bits (61), Expect = 3.8
Identities = 20/68 (29%), Positives = 32/68 (47%), Gaps = 11/68 (16%)
Frame = -2
Query: 310 ETVEQMLIAVQEPHKIDVLLSGFS-----------YLSIESLHSSTCSSCVLTRGGNNPW 164
E V L +V+ P DV+++GF+ Y + H S C++CV + PW
Sbjct: 115 EWVTSKLGSVKLPRTKDVMVNGFTVKVKFCDTCQLYRPPRAFHCSICNNCVQRFDHHCPW 174
Query: 163 IPKTDALR 140
+ + ALR
Sbjct: 175 VGQCIALR 182
>At1g80600.1 68414.m09457 acetylornithine aminotransferase,
mitochondrial, putative / acetylornithine transaminase,
putative / AOTA, putative / ACOAT, putative similar to
SP|O04866 Acetylornithine aminotransferase,
mitochondrial precursor (EC 2.6.1.11) (ACOAT)
(Acetylornithine transaminase) (AOTA) {Alnus glutinosa};
contains Pfam profile PF00202: aminotransferase, class
III
Length = 457
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/42 (33%), Positives = 23/42 (54%)
Frame = -3
Query: 675 EPDVQRVVQHRLGVRTHVDSDGQTLMRLDASERGVEREFAHR 550
+PD R V + GV HV + T+ +++ ++R V FA R
Sbjct: 112 DPDWLRAVTEQAGVLAHVSNVYYTIPQIELAKRLVASSFADR 153
>At5g44040.1 68418.m05389 expressed protein similar to unknown
protein (gb|AAD10667.1)
Length = 416
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +2
Query: 548 SLWANSRSTPRSEASSRISVCPSLSTWVRTPSRCWTTRCTS 670
SL SR + + I+V SLSTW+ T S+ T+ C+S
Sbjct: 302 SLNRKSRDETEKQRTQEIAVDASLSTWLST-SQTTTSGCSS 341
>At1g04780.1 68414.m00474 ankyrin repeat family protein contains
Pfam PF00023: Ankyrin repeat
Length = 664
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +2
Query: 566 RSTPRSEASSRISVCPSLSTWVRTPSRCWTTRCTSG 673
+++PR E S + S W++TPS+ +T +SG
Sbjct: 582 KNSPREETQSSSNPSSSWFQWIKTPSQRPSTSSSSG 617
>At5g64430.1 68418.m08093 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein contains Pfam profile PF00564:
PB1 domain
Length = 513
Score = 27.5 bits (58), Expect = 8.7
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -1
Query: 590 MPPSAV*SASLPTGMPIPHAPKSPRPRNVLR 498
+P V P+ P+ HAP P+P+ V+R
Sbjct: 336 LPEQPVYMIPSPSPSPVYHAPPPPQPQGVIR 366
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,348,533
Number of Sequences: 28952
Number of extensions: 413604
Number of successful extensions: 1269
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1268
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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