Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0663
(525 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g12190.1 68416.m01520 hypothetical protein 35 0.029
At4g38070.1 68417.m05377 bHLH family protein contains Pfam profi... 32 0.27
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 31 0.63
At3g14205.1 68416.m01795 phosphoinositide phosphatase family pro... 29 1.4
At1g79020.1 68414.m09214 transcription factor-related similar to... 29 1.9
At5g41110.1 68418.m04998 expressed protein ; expression support... 29 2.5
At4g01350.1 68417.m00175 DC1 domain-containing protein contains ... 29 2.5
At2g41910.1 68415.m05185 protein kinase family protein contains ... 28 3.3
At2g31840.1 68415.m03888 expressed protein 28 4.4
At3g46050.1 68416.m04983 kelch repeat-containing F-box family pr... 27 5.8
At3g42850.1 68416.m04489 galactokinase, putative contains some s... 27 5.8
At3g22020.1 68416.m02778 receptor-like protein kinase-related co... 27 5.8
At1g52080.1 68414.m05875 actin binding protein family contains P... 27 5.8
At4g35910.1 68417.m05103 expressed protein 27 7.7
At3g28960.1 68416.m03620 amino acid transporter family protein l... 27 7.7
At1g77010.1 68414.m08968 pentatricopeptide (PPR) repeat-containi... 27 7.7
At1g67150.1 68414.m07639 hypothetical protein contains Pfam prof... 27 7.7
At1g60570.1 68414.m06819 kelch repeat-containing F-box family pr... 27 7.7
At1g19360.1 68414.m02409 expressed protein 27 7.7
At1g12030.1 68414.m01389 expressed protein contains Pfam profile... 27 7.7
>At3g12190.1 68416.m01520 hypothetical protein
Length = 269
Score = 35.1 bits (77), Expect = 0.029
Identities = 22/61 (36%), Positives = 33/61 (54%)
Frame = +1
Query: 253 KKQNIMEALTNYRDKETSLRHFEMILQEKQYEHNLLRAEWDKSLGELRDMKAAVSDDEEM 432
KK+N++ N + E L+H L+EKQ E +L+ +K LGE RD+K + D
Sbjct: 213 KKKNLVLCKRN-EEAERKLKHLNRALEEKQKEVDLI----EKRLGEWRDVKGRGNGDTSG 267
Query: 433 D 435
D
Sbjct: 268 D 268
>At4g38070.1 68417.m05377 bHLH family protein contains Pfam profile:
PF00010 helix-loop-helix DNA-binding domain; PMID:
12679534; putative bHLH131 transcription factor
Length = 1513
Score = 31.9 bits (69), Expect = 0.27
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +1
Query: 298 ETSLRHFEMILQEKQYEHNLLRAEWDK 378
E SL+ ++LQ+KQ E N+LR W+K
Sbjct: 857 EGSLKSVSLLLQQKQNEVNMLRKTWEK 883
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 30.7 bits (66), Expect = 0.63
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +1
Query: 331 QEKQYEHNLLRAEWDKSLGELRDMKAAVSDDEEMDTNSAYYVRLRTLVSK 480
+E++ + +AE K + KA +DDEEMD Y RL+ L ++
Sbjct: 52 EEEKRRKDEEKAEKAKQAPKASSQKAVAADDEEMDATQYYENRLKYLAAE 101
>At3g14205.1 68416.m01795 phosphoinositide phosphatase family
protein contains similarity to phosphoinositide
phosphatase SAC1 [Rattus norvegicus]
gi|11095248|gb|AAG29810; contains Pfam domain, PF02383:
SacI homology domain; identical to SAC domain protein 2
(SAC2) GI:31415720
Length = 808
Score = 29.5 bits (63), Expect = 1.4
Identities = 13/51 (25%), Positives = 26/51 (50%)
Frame = -1
Query: 153 LIAATSPYSLMISSVLTCSVICGVSDTGFALRSLSLEENRDSSCSFFETAS 1
++ + Y+L ++S+ C + + GF ++ S EN D CS ++ S
Sbjct: 401 ILGRLATYALNLTSIFYCQLTPDLRGEGFQNQNPSTLENDDGECSTYDPPS 451
>At1g79020.1 68414.m09214 transcription factor-related similar to
enhancer of polycomb (GI:3757890) [Drosophila
melanogaster]; similar to enhancer of polycomb
(GI:11907923) [Homo sapiens]
Length = 453
Score = 29.1 bits (62), Expect = 1.9
Identities = 23/76 (30%), Positives = 36/76 (47%), Gaps = 6/76 (7%)
Frame = +1
Query: 259 QNIMEALTNYRDKETSLRHFEMILQ--EKQYEHNLLRAEWDKSLGELR----DMKAAVSD 420
Q+I+EAL +K+ + E+ LQ + QY H E ++ + K SD
Sbjct: 258 QSILEALIKREEKKRDVMDSEVSLQRIQLQYRHETELLEDSLAMPGFQPTTTSYKFGSSD 317
Query: 421 DEEMDTNSAYYVRLRT 468
DE MD++ R+RT
Sbjct: 318 DELMDSDDHTSTRVRT 333
>At5g41110.1 68418.m04998 expressed protein ; expression supported
by MPSS
Length = 621
Score = 28.7 bits (61), Expect = 2.5
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +1
Query: 151 QIIREGHCEMRCTNTCCKKTYNDTGQ 228
+++ E H C N C++TY +TGQ
Sbjct: 516 ELVDELHSPNECGNKNCRETYRETGQ 541
>At4g01350.1 68417.m00175 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 652
Score = 28.7 bits (61), Expect = 2.5
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = -1
Query: 153 LIAATSPYSLMISSV-LTCSVICGVSDTGFALRSLSLE 43
L+ T PY+LM + LT +CG+ GFA S L+
Sbjct: 428 LVFGTRPYTLMKETTQLTDCTLCGIFSDGFAYSSRWLD 465
>At2g41910.1 68415.m05185 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 373
Score = 28.3 bits (60), Expect = 3.3
Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 3/42 (7%)
Frame = -1
Query: 519 KQPISYKPPYHL-HLRDQGSKA--YIICTVCVHFLVITDCCL 403
+ P S P H+ HL K +I C++CV F+++T C+
Sbjct: 330 RAPRSNLLPVHICHLAQPSQKICFFIYCSLCVVFIILTVVCM 371
>At2g31840.1 68415.m03888 expressed protein
Length = 350
Score = 27.9 bits (59), Expect = 4.4
Identities = 13/48 (27%), Positives = 25/48 (52%)
Frame = +1
Query: 292 DKETSLRHFEMILQEKQYEHNLLRAEWDKSLGELRDMKAAVSDDEEMD 435
D+E ++ +E + + R EW K++ E+ D + +DEE+D
Sbjct: 87 DEEDEVKEETFGGKEASLDDPVERREWRKTIREVIDKHPDIEEDEEID 134
>At3g46050.1 68416.m04983 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 370
Score = 27.5 bits (58), Expect = 5.8
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = -1
Query: 294 ISIVCQGFHYILFLVSISASRALSGVVISFFTACV 190
+S+VC+GF +L + A+R+ G SF C+
Sbjct: 41 LSLVCKGFRSLLDSRELHATRSCIGKTESFLYVCL 75
>At3g42850.1 68416.m04489 galactokinase, putative contains some
similarity to galactokinase [Pasteurella multocida]
SWISS-PROT:P57899
Length = 964
Score = 27.5 bits (58), Expect = 5.8
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 2/32 (6%)
Frame = -1
Query: 522 DKQPISYKPPYHLHLRDQGSK--AYIICTVCV 433
D +PISY YH RD K AY+ T+ V
Sbjct: 572 DGKPISYDKAYHYFSRDPSQKWAAYVAGTILV 603
>At3g22020.1 68416.m02778 receptor-like protein kinase-related
contains Pfam profile: PF01657 Domain of unknown
function; weak similarity to receptor-like protein
kinase 5 (GI:13506747) [Arabidopsis thaliana]; weak
similarity to receptor-like protein kinase homolog
RK20-1 (GI:4530126) [Phaseolus vulgaris]
Length = 223
Score = 27.5 bits (58), Expect = 5.8
Identities = 15/41 (36%), Positives = 26/41 (63%), Gaps = 1/41 (2%)
Frame = -1
Query: 192 VCATHFTVTFSNNLIAATSPYSLMISSVLTCSVICG-VSDT 73
+C T+ ++ N A + +L+ISS+++ S+I G VSDT
Sbjct: 3 ICTTNASLVKGNISREARTRKTLIISSIISLSIIFGTVSDT 43
>At1g52080.1 68414.m05875 actin binding protein family contains
Prosite PS00019: Actinin-type actin-binding domain
signature 1; similar to actin binding protein
(GI:28071265) [Arabidopsis thaliana]; similar to A-type
inclusion protein (ATI) (Swiss-Prot:P16602) [Cowpox
virus]
Length = 573
Score = 27.5 bits (58), Expect = 5.8
Identities = 21/69 (30%), Positives = 36/69 (52%)
Frame = +1
Query: 304 SLRHFEMILQEKQYEHNLLRAEWDKSLGELRDMKAAVSDDEEMDTNSAYYVRLRTLVSKM 483
SL+ LQE++ + L E DK + LRD+++ + +E DTN+ L K+
Sbjct: 247 SLKQRVARLQEEEIKAVLPDLEADKMMQRLRDLESEI--NELTDTNTRLQFENFELSEKL 304
Query: 484 EMIRWLIGN 510
E ++ +I N
Sbjct: 305 ESVQ-IIAN 312
>At4g35910.1 68417.m05103 expressed protein
Length = 458
Score = 27.1 bits (57), Expect = 7.7
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = -1
Query: 108 LTCSVICGVSDTGFALRSLSLEENRDSSCSFFETAS 1
L C CG++D + +L EEN C+ TA+
Sbjct: 291 LACRSQCGINDLVSSFVALLQEENPSRECTIVRTAA 326
>At3g28960.1 68416.m03620 amino acid transporter family protein low
similarity to vesicular inhibitory amino acid
transporter [Mus musculus] GI:2826776; contains
INTERPRO:IPR002422 amino acid/polyamine transporter,
family II
Length = 405
Score = 27.1 bits (57), Expect = 7.7
Identities = 26/91 (28%), Positives = 38/91 (41%)
Frame = -1
Query: 324 HLKMTKTGFLISIVCQGFHYILFLVSISASRALSGVVISFFTACVCATHFTVTFSNNLIA 145
HL + T F+IS V F +S AL V +S C+C + F N
Sbjct: 317 HL-LISTFFIISSVVIAETLPFFGYMMSLVGALLSVTVSILLPCLC---YLKIFGN--YK 370
Query: 144 ATSPYSLMISSVLTCSVICGVSDTGFALRSL 52
++M+ ++ SV GV T ALR +
Sbjct: 371 KIGCETIMLFGMVVMSVFVGVIGTYIALRDI 401
>At1g77010.1 68414.m08968 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 695
Score = 27.1 bits (57), Expect = 7.7
Identities = 15/43 (34%), Positives = 26/43 (60%)
Frame = +1
Query: 265 IMEALTNYRDKETSLRHFEMILQEKQYEHNLLRAEWDKSLGEL 393
++E N +K TSLR F+M+ + Y N++ + + K+ GEL
Sbjct: 99 MIEGYMNSGEKGTSLRFFDMMPERDGYSWNVVVSGFAKA-GEL 140
>At1g67150.1 68414.m07639 hypothetical protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 408
Score = 27.1 bits (57), Expect = 7.7
Identities = 15/70 (21%), Positives = 35/70 (50%)
Frame = +1
Query: 316 FEMILQEKQYEHNLLRAEWDKSLGELRDMKAAVSDDEEMDTNSAYYVRLRTLVSKMEMIR 495
++M+ +K + D + L DMK + DEE++ ++Y V ++S + +
Sbjct: 50 YKMMEDQKVLYKDAFTKRLDTDIAILEDMKTTIK-DEEVNIRASYAVSTAWILSDI-FVE 107
Query: 496 WLIGNWLFVV 525
++ + +F+V
Sbjct: 108 LILNDSIFIV 117
>At1g60570.1 68414.m06819 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 381
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = -1
Query: 294 ISIVCQGFHYILFLVSISASRALSGVVISFFTACVCATH 178
+S+VC+ FH +L I R+LSG + C+ +H
Sbjct: 45 LSLVCKRFHSLLTSGEIYRFRSLSGYTENCLYVCLRFSH 83
>At1g19360.1 68414.m02409 expressed protein
Length = 428
Score = 27.1 bits (57), Expect = 7.7
Identities = 16/53 (30%), Positives = 22/53 (41%)
Frame = -1
Query: 246 ISASRALSGVVISFFTACVCATHFTVTFSNNLIAATSPYSLMISSVLTCSVIC 88
+ SR ++I F CVCA F F N+ + + L SS S C
Sbjct: 11 LRGSRIAIAILIGIFIGCVCAVLFPYGFFNSSSSLKASEHLSKSSNQVGSSAC 63
>At1g12030.1 68414.m01389 expressed protein contains Pfam profile
PF04720: Protein of unknown function (DUF506)
Length = 295
Score = 27.1 bits (57), Expect = 7.7
Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 1/82 (1%)
Frame = +1
Query: 253 KKQNIM-EALTNYRDKETSLRHFEMILQEKQYEHNLLRAEWDKSLGELRDMKAAVSDDEE 429
++Q IM EA+ R RHF L+ K ++ L ++ W+K G+ K D +
Sbjct: 92 ERQRIMDEAVNASRVFAGEKRHFMAYLRNKGFDAGLCKSRWEK-FGKNTAGKYEYVDVKA 150
Query: 430 MDTNSAYYVRLRTLVSKMEMIR 495
D N Y+ L + E+ R
Sbjct: 151 GDKNR--YIVETNLAGEFEIAR 170
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,360,822
Number of Sequences: 28952
Number of extensions: 222523
Number of successful extensions: 708
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 690
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 708
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 967280384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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